...region of a gene (cDNA) can be done by either plasmid or virus. A plasmid carrying your cDNA can be targeted...to integrate your choice cDNA. Both lentivirus and retrovirus can package cDNAs for genomic delivery and...to choose an appropriate promoter to express your cDNA, depending on the level of expression you would ...

...For this example, we will describe how to copy a cDNA from one vector into a new vector that is better...example, we will use EcoRI and NotI to ligate our cDNA into the recipient plasmid. Remember to insert your...from plasmid DNA (rather than from genomic DNA or a cDNA library), roughly 18-21bp is usually sufficient ...Tm if you are trying to clone from genomic DNA, a cDNA library, or by RT-PCR. Isolate the PCR product Isolate...

... set of one to three mutant complementary DNAs (cDNAs) representing core nodes that, when over-expressed...curate the perfect library, obtained the appropriate cDNAs from other researchers, and cloned them into lentiviral...months from start to finish. They cloned all the cDNAs into a single consensus lentiviral expression vector...

...simpler to implement in genome-wide screens over cDNA libraries. However, using CRISPRa to target individual...highlight a key advantage of CRISPRa over traditional cDNA overexpression: isoform-specific gene expression...— at least, more straightforward than generating cDNA for each gene isoform individually. To highlight...application can be found here.  Why CRISPRa? Although cDNA overexpression approaches may still be preferable...strategies) and then consider whether traditional cDNA overexpression is suited to your experimental goals...

...large scale, broadly available collection of mutant cDNA clones corresponding to cancer variants found in... genome sequencing studies (as well as reference cDNA clones mapping to well-annotated cellular pathways...Addgene. These include 634 sequence confirmed mutant cDNA clones corresponding to cancer variants derived ...

...they want to activate, we would recommend using a cDNA overexpression vector rather than going through ...protein structure and function (Pan et al., 2018). cDNA libraries, which consist of plasmids that over-express...and deliver when compared to gRNAs. Additionaly, cDNAs cannot be used to study in cis regulation and also...

...complementary DNA (cDNA), and then use PCR and molecular cloning to insert the cDNA into the desired plasmid...

...requires RNA to be converted to cDNA using reverse transcription and the cDNA is subsequently quantified by...

..., reverse transcription of RNA such as long-read cDNA sequencing and RNA sequencing of complex heterogeneous...

... from the publication is the removal of unwanted cDNA derived from 16S rRNA in bacterial RNA-Seq. This...

...myeloma cell. This RNA was then used to generate cDNA, which  was used as a template for PCR amplification...

...expression construct, to cells/organisms. This is often cDNA, shRNA, or something similar, and is contained on...

...In this approach, viral RNA is first converted to cDNA and then quantified using qPCR primers targeting...

...adaptable to cloning different types of DNA, such as cDNA, PCR fragments, or genomic DNA. Also remember that...

...sybody candidates are then reverse transcribed into cDNA fragments that are efficiently subcloned into the...

...over- and under-expressing clones. If GFP-encoding cDNA is co-transfected at a favorable ratio to the gene...

...as shown by Dalvai et al., who used HDR to insert cDNA constructs into this locus. One important question...

... created for a variety of applications including cDNA expression, shRNA-mediated knockdown, Tet and Cre-regulated...

...of these species and can often be used to clone cDNAs from them. These enzymes might not, however, be ...

...types of DNA fragments may be cloned: PCR fragments, cDNA or Genomic DNA and is available for all kind of ...