...For this example, we will describe how to copy a cDNA from one vector into a new vector that is better...example, we will use EcoRI and NotI to ligate our cDNA into the recipient plasmid. Remember to insert your...from plasmid DNA (rather than from genomic DNA or a cDNA library), roughly 18-21bp is usually sufficient ...Tm if you are trying to clone from genomic DNA, a cDNA library, or by RT-PCR. Isolate your PCR product ...

...purposes of this tutorial we will discuss how to move a cDNA from one plasmid to another. However, the same technique... problem is that the only version of full-length cDNA you can find for YGOI is in a bacterial expression...