We narrowed to 54,294 results for: Ran

1. pGP-CMV-jGCaMP8f F366H

   Plasmid

   #204141

   Purpose

   Fluorescent reporter for calcium imaging in mammalian cells - jGCaMP8f with F366H mutation

   Depositor

   Katalin Torok

   Article

   Tran et al Front Cell Neurosci. 2023 May 18;17:1155

   Insert

   jGCaMP8f F366H
   

   Use

   Tags

   N-terminal His tag

   Expression

   Mammalian

   Mutation

   Phenylalanine 366 changed to histidine

   Promoter

   CMV

   Available since

   Dec. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. GST-GAR

   Plasmid

   #34697

   Depositor

   Steven Clarke

   Article

   Frankel et al J Biol Chem. 2000 Oct 20;275(42):32974-8

   Insert

   Fibrillarin (FBL Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Residues 1-145 of human fibrillarin with K2E and …

   Promoter

   tac

   Available since

   July 12, 2012

   Availability

   Academic Institutions and Nonprofits only

3. Bxb1-ASAP2f

   Plasmid

   #183757

   Purpose

   Bxb1-specific donor plasmid for intergrating a transcription unit for expression of ASAP2f voltage reporter

   Depositor

   Richard Davis

   Article

   Blanch-Asensio et al Cell Rep Methods. 2022 Sep 22;2(10):1003

   Insert

   ASAP2f
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pWZL Blast H-Ras G12V T35S

   Plasmid

   #12278

   Depositor

   Bob Weinberg

   Article

   Rangarajan et al Cancer Cell. 2004 Aug . 6(2):171-83.

   Insert

   H-Ras G12V T35S (HRAS Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Contains G12V activating mutation. The T35S mutat…

   Promoter

   Available since

   July 20, 2006

   Availability

   Academic Institutions and Nonprofits only

5. pLuc-CDS-m215-2

   Plasmid

   #42102

   Depositor

   Kumiko Ui-Tei

   Article

   Mazda et al PLoS One. 2011;6(12):e28688. doi: 10.137

   Insert

   ZEB1 (ZEB1 Human)
   

   Use

   Luciferase

   Tags

   Renilla luciferase

   Expression

   Mammalian

   Mutation

   Silent mutations were introduced in the seed-comp…

   Promoter

   SV40

   Available since

   March 6, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBTK601

   Plasmid

   #110607

   Purpose

   BTK assembled plasmid - encodes Cas9 with no targeting sgRNA (used with suicide plasmid for broad-host-range genome alteration)

   Depositor

   Jeffrey Barrick

   Article

   Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

   Insert

   Cas9
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMS6: pHelper(AvCAST)_entry_ΔTnsD

   Plasmid

   #168139

   Purpose

   Inducible expression of AvCAST proteins (except TnsD). Two BsaI sites for spacer cloning.

   Depositor

   Feng Zhang

   Article

   Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.

   Inserts

   AvCAST minimal CRISPR array
   AvCAST Cascade proteins (Cas6, Cas8, Cas7 and Cas5)
   AvCAST Tns proteins (TnsA, TnsB, TnsC and TniQ)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. Bxb1_jRCaMP1b

   Plasmid

   #183758

   Purpose

   Bxb1-specific donor plasmid for intergrating a transcription unit for expression of jRCaMP1b calcium reporter

   Depositor

   Richard Davis

   Article

   Blanch-Asensio et al Cell Rep Methods. 2022 Sep 22;2(10):1003

   Insert

   jRCaMP1b
   

   Use

   Tags

   NES

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pWZL Blast H-Ras G12V E37G

   Plasmid

   #12277

   Depositor

   Bob Weinberg

   Article

   Rangarajan et al Cancer Cell. 2004 Aug . 6(2):171-83.

   Insert

   H-Ras G12V E37G (HRAS Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Contains G12V activating mutation. The E37G mutat…

   Promoter

   Available since

   Aug. 3, 2006

   Availability

   Academic Institutions and Nonprofits only

10. pWZL Blast H-Ras G12V Y40C

    Plasmid

    #12279

    Depositor

    Bob Weinberg

    Article

    Rangarajan et al Cancer Cell. 2004 Aug . 6(2):171-83.

    Insert

    H-Ras G12V Y40C (HRAS Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Contains G12V activating mutation. The Y40C mutat…

    Promoter

    Available since

    July 20, 2006

    Availability

    Academic Institutions and Nonprofits only

11. pEPQD1CB0107

    Plasmid

    #177040

    Purpose

    MoClo Level 1, position 1, transcriptional unit for transient expression of 1-deoxy-D-xylulose 5-phosphate synthase (CrDXS2) from Catharanthus roseus driven by 35S promoter; contains a chloroplast transit peptide

    Depositor

    Nicola Patron

    Article

    Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

    Insert

    1-deoxy-D-xylulose 5-phosphate synthase (CrDXS2) from Catharanthus roseus
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pVENUS-N1-FNTA

    Plasmid

    #86859

    Purpose

    To express FNTA with a Venus tag at C terminus.

    Depositor

    Yihong Ye

    Article

    Xu et al Cell Discov. 2016 Nov 8;2:16040. eCollec

    Insert

    FNTA (FNTA Human)
    

    Use

    Tags

    Venus

    Expression

    Mammalian

    Mutation

    The sequence of the insert is isoform 2, which di…

    Promoter

    CMV

    Available since

    April 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. Nanog-t2A-mCherry

    Plasmid

    #127541

    Purpose

    Expresses the transactivation domain of mouse Nanog and mCherry via t2A linker.

    Depositor

    Jennifer Mitchell

    Article

    Dhaliwal et al Genes Dev. 2019 Jun 20. pii: gad.324319.

    Insert

    Nanog (Nanog Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Codes for the last 110 a.a of Nanog

    Promoter

    CMV

    Available since

    July 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAC1-pCR8-dCas9VP48

    Plasmid

    #48214

    Purpose

    dCas9VP48 on Gateway donor vector pCR8/GW/TOPO. Note: This is not for expression. It has to be transferred to a gateway destination vector for expression

    Depositor

    Rudolf Jaenisch

    Article

    Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

    Insert

    dCas9(D10A;H840A) fusion with VP48 activation domain
    

    Use

    CRISPR; Gateway donor

    Tags

    HA Tag and VP48

    Expression

    Mutation

    D10A;H840A

    Promoter

    Available since

    Sept. 17, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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15. pUBC(k)-AT5G42080-mKOk

    Plasmid

    #224852

    Purpose

    Plasmid for expression of AT5G40280.1 coding sequence tagged with mKOk in plants

    Depositor

    Rebecca Roston

    Article

    LaBrant et al bioRxiv 2025.06.20.660776; doi: https://

    Insert

    AT5G40280.1 (ERA1 Mustard Weed)
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    July 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pOPINF AtMKK5

    Plasmid

    #228396

    Purpose

    Recombinant expression of His6-tagged Arabidopsis thaliana MKK5 in E. coli

    Depositor

    Lennart Wirthmueller

    Article

    Simranjit et al bioRxiv 2023.09.25.558804

    Insert

    MKK5 (MKK5 Mustard Weed)
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    March 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pENTR4 HopU1

    Plasmid

    #228395

    Purpose

    pENTR4 plasmid with CDS of Pseudomonas syringae pv tomato type III effector HopU1 without stop codon for C-terminal epitope tags

    Depositor

    Lennart Wirthmueller

    Article

    Simranjit et al bioRxiv 2023.09.25.558804

    Insert

    HopU1
    

    Use

    Gateway-compatible entry vector

    Tags

    Expression

    Mutation

    Alanine inserted after start Methionine due to Nc…

    Promoter

    Available since

    Feb. 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. Stargazin-EBFP2-FRB

    Plasmid

    #222417

    Purpose

    To express a plasma membrane "anchor" for rapamycin-induced heterodimerisation in mammalian cells

    Depositor

    Stephen Royle

    Article

    Downie et al PLoS Biol. 2025 Jul 10;23(7):e3003249. d

    Insert

    Cacng2 (Cacng2 Mouse)
    

    Use

    Tags

    EBFP2-HA-FRB

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET30b jGCaMP8f L27A

    Plasmid

    #204138

    Purpose

    Production of recombinant calcium sensor jGCaMP8f L27A fast decay variant in E.coli

    Depositor

    Katalin Torok

    Article

    Tran et al Front Cell Neurosci. 2023 May 18;17:1155

    Insert

    jGCaMP8f L27A
    

    Use

    Tags

    N-terminal His tag

    Expression

    Bacterial

    Mutation

    Leucine 27 changed to Alanine

    Promoter

    T7

    Available since

    Dec. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pGP-CMV-jGCaMP8f L27A

    Plasmid

    #204140

    Purpose

    Fluorescent reporter for calcium imaging in mammalian cells - jGCaMP8f with L27A mutation

    Depositor

    Katalin Torok

    Article

    Tran et al Front Cell Neurosci. 2023 May 18;17:1155

    Insert

    jGCaMP8f L27A
    

    Use

    Tags

    N-terminal His tag

    Expression

    Mammalian

    Mutation

    Leucine 27 changed to Alanine

    Promoter

    CMV

    Available since

    Dec. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge