We narrowed to 9,396 results for: pho;

1. CPH3508 (YCp LEU2 Rpb1 Y1473A)

   Plasmid

   #91811

   Purpose

   Yeast expression of S. cerevisiae Rpb1 with a Y1473A mutation

   Depositor

   Chris Hill

   Article

   Sdano et al Elife. 2017 Aug 16;6. pii: 28723. doi: 1

   Insert

   RPO21 (RPO21 Budding Yeast)
   

   Expression

   Yeast

   Mutation

   changed tyrosine 1473 to alanine

   Promoter

   Endogenous Rpb1 promoter

   Available Since

   March 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. CPH3509 (YCp LEU2 Rpb1 T1471A)

   Plasmid

   #91812

   Purpose

   Yeast expression of S. cerevisiae Rpb1 with a T1471A mutation

   Depositor

   Chris Hill

   Article

   Sdano et al Elife. 2017 Aug 16;6. pii: 28723. doi: 1

   Insert

   RPO21 (RPO21 Budding Yeast)
   

   Expression

   Yeast

   Mutation

   changed threonine 1471 to alanine

   Promoter

   Endogenous Rpb1 promoter

   Available Since

   March 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. CPH3511 (YCp LEU2 Rpb1 P1472A)

   Plasmid

   #91813

   Purpose

   Yeast expression of S. cerevisiae Rpb1 with a P1472A mutation

   Depositor

   Chris Hill

   Article

   Sdano et al Elife. 2017 Aug 16;6. pii: 28723. doi: 1

   Insert

   RPO21 (RPO21 Budding Yeast)
   

   Expression

   Yeast

   Mutation

   changed proline 1472 to alanine

   Promoter

   Endogenous Rpb1 promoter

   Available Since

   March 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. CPH3567 (pET151_Spt6 1247-1451 R1282H)

   Plasmid

   #91816

   Purpose

   Bacterial expression of residues 1247-1451 from S. cerevisiae Spt6 with an R1282H mutation

   Depositor

   Chris Hill

   Article

   Sdano et al Elife. 2017 Aug 16;6. pii: 28723. doi: 1

   Insert

   SPT6 (SPT6 Budding Yeast)
   

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   changed arginine 1282 to histidine; deleted resid…

   Promoter

   T7

   Available Since

   March 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. CPH3495 (pET151_Spt6 1247-1451 K1435A)

   Plasmid

   #91817

   Purpose

   Bacterial expression of residues 1247-1451 from S. cerevisiae Spt6 with a K1435A mutation

   Depositor

   Chris Hill

   Article

   Sdano et al Elife. 2017 Aug 16;6. pii: 28723. doi: 1

   Insert

   SPT6 (SPT6 Budding Yeast)
   

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   changed lysine 1435 to alanine; deleted residues …

   Promoter

   T7

   Available Since

   March 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. KIF13B

   Plasmid

   #163907

   Purpose

   Full length mouse KIF13B for purification from insect cells

   Depositor

   Martin Loose

   Article

   Duellberg et al Proc Natl Acad Sci U S A. 2021 Jan 5;118

   Insert

   kif13b (Kif13b Mouse)
   

   Tags

   Cleavable 6 x HIS tag

   Expression

   Insect

   Promoter

   polyhedrin

   Available Since

   March 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. KIF13B 1-584

   Plasmid

   #163908

   Purpose

   Motile fragment of mouse KIF13B (aa 1-584) for purification from insect cells.

   Depositor

   Martin Loose

   Article

   Duellberg et al Proc Natl Acad Sci U S A. 2021 Jan 5;118

   Insert

   kif13b (Kif13b Mouse)
   

   Tags

   Cleavable 6 x HIS tag

   Expression

   Insect

   Promoter

   polyhedrin

   Available Since

   March 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHA853 - mec-4p::Fynempty

   Plasmid

   #139210

   Purpose

   Control vector for Fyn kinase expression in mec-4 neurons

   Depositor

   Anne Hart

   Article

   Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

   Insert

   Fyn (FYN Human)
   

   Expression

   Worm

   Mutation

   deletion and frame shift to prevent Fyn expression

   Available Since

   Dec. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pHA850 - osm-10p::FynY531F

   Plasmid

   #139207

   Purpose

   Express activated Fyn kinase in osm-10 neurons

   Depositor

   Anne Hart

   Article

   Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

   Insert

   Fyn (FYN Human)
   

   Expression

   Worm

   Mutation

   Y531F

   Available Since

   Dec. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pHA851 - osm-10p::Fynempty

    Plasmid

    #139208

    Purpose

    Control vector for Fyn kinase expression in osm-10 neurons

    Depositor

    Anne Hart

    Article

    Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

    Insert

    Fyn (FYN Human)
    

    Expression

    Worm

    Mutation

    deletion and frame shift to prevent Fyn expression

    Available Since

    Dec. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. FUS_LC_withR

    Plasmid

    #139128

    Purpose

    express FUS LC with the same number and spacing of arginines as hnRNPA2 LC

    Depositor

    Nicolas Fawzi

    Article

    Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

    Insert

    FUS (FUS Human)
    

    Expression

    Bacterial

    Mutation

    add arginines

    Available Since

    Dec. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. hnRNPA2_LC_nocharge_wR

    Plasmid

    #139119

    Purpose

    express hnRNPA2 LC with no charged residues with R added back

    Depositor

    Nicolas Fawzi

    Article

    Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

    Insert

    hnRNPA2 (HNRNPA2B1 Human)
    

    Expression

    Bacterial

    Mutation

    remove all K, most D

    Available Since

    Dec. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. FUS_LC_charge

    Plasmid

    #139126

    Purpose

    express FUS LC with an hnRNPA2 LC-like charge patterning

    Depositor

    Nicolas Fawzi

    Article

    Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

    Insert

    FUS (FUS Human)
    

    Expression

    Bacterial

    Mutation

    Add R, K, D to make FUS LC have similar charge pa…

    Available Since

    Dec. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLenti-CMV-TIRAP-cGASΔN-HA

    Plasmid

    #130921

    Purpose

    Expresses human cGASΔN (aa160-522)-HA with an N terminal fusion of the TIRAP N terminus (aa1-85); Puromycin selection marker

    Depositor

    Jonathan Kagan

    Article

    Barnett et al Cell. 2019 Mar 7;176(6):1432-1446.e11. d

    Insert

    TIRAP-ΔNcGAS
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    cGAS N terminus (aa1-159) replaced with the N ter…

    Promoter

    CMV

    Available Since

    Aug. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET151-GST-PP-RPN13 (19-132) R104A

    Plasmid

    #125826

    Purpose

    Expresses GST-RPN13 (19-132) R104A in E.coli

    Depositor

    Chris Hill

    Article

    Hemmis et al J Biol Chem. 2019 May 7. pii: AC119.0088

    Insert

    RPN13 (ADRM1 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    changed arginine 104 to alanine

    Promoter

    T7

    Available Since

    May 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET151-GST-PP-RPN13 (19-132) R104D

    Plasmid

    #125827

    Purpose

    Expresses GST-RPN13 (19-132) R104D in E.coli

    Depositor

    Chris Hill

    Article

    Hemmis et al J Biol Chem. 2019 May 7. pii: AC119.0088

    Insert

    RPN13 (ADRM1 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    changed arginine 104 to aspartate

    Promoter

    T7

    Available Since

    May 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET151-GST-PP-RPN13 (19-132) R104E

    Plasmid

    #125828

    Purpose

    Expresses GST-RPN13 (19-132) R104E in E.coli

    Depositor

    Chris Hill

    Article

    Hemmis et al J Biol Chem. 2019 May 7. pii: AC119.0088

    Insert

    RPN13 (ADRM1 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    changed arginine 104 to glutamate

    Promoter

    T7

    Available Since

    May 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. Gal4-HSF4b K294R

    Plasmid

    #118359

    Purpose

    This plasmid expresses amino-acids 200-493 of hHSF4b K294R mutant fused to the GAL-4 DNA binding domain. This construct cannot undergo sumoylation on K294.

    Depositor

    Lea Sistonen

    Article

    Hietakangas et al Proc Natl Acad Sci U S A. 2006 Jan 3;103

    Insert

    hHSF4b (HSF4 Human)
    

    Expression

    Mammalian

    Mutation

    K294R

    Available Since

    Nov. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. Flag-KSR R615H

    Plasmid

    #118332

    Purpose

    Expression of mouse KSR

    Depositor

    Philip Stork

    Article

    Takahashi et al J Biol Chem. 2017 Jan 27;292(4):1449-146

    Insert

    KSR R615H (Ksr1 Mouse)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    R615H

    Available Since

    Nov. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. hHSF1 S307A

    Plasmid

    #118364

    Purpose

    This plasmid expresses human HSF1 S307A mutant.

    Depositor

    Lea Sistonen

    Article

    Hietakangas et al Mol Cell Biol. 2003 Apr . 23(8):2953-68.

    Insert

    hHSF1 (HSF1 Human)
    

    Tags

    myc and 6xHIS

    Expression

    Mammalian

    Mutation

    S307A

    Available Since

    Nov. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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