We narrowed to 31,919 results for: Eng

1. pCMV-MbPylRS(DiZPK)

   Plasmid

   #91706

   Purpose

   express Methanosarcina barkeri pyrrolysyl-tRNA synthetase/tRNA pair in mammalian cells to incorporate the photocrosslinkers DiZPK, DiZSeK, or DiZHSeC into amber codon site on a protein of interest

   Depositor

   P. Chen

   Article

   Lin et al Angew Chem Int Ed Engl. 2013 Dec 23;52(5

   Inserts

   pylS
   pylT
   

   Expression

   Mammalian

   Mutation

   T810G, C813T, C820G, T821C, T937A, A1046T, G1257A

   Available Since

   July 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-pCALM1-EYFP-WPRE-bGHpA

   Plasmid

   #213971

   Purpose

   AAV expression of EYFP from pCALM1 promoter

   Depositor

   Zhonghua Lu

   Article

   Wang et al Cell Rep. 2023 Nov 28;42(11):113348. doi

   Insert

   pCALM1
   

   Use

   AAV

   Available Since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCAG-G-Flamp1-mut

   Plasmid

   #188568

   Purpose

   control probe of G-Flamp1, insensitive to cAMP

   Depositor

   Jun Chu

   Article

   Wang et al Nat Commun. 2022 Sep 12;13(1):5363. doi:

   Insert

   G-Flamp1-mut
   

   Tags

   His tag

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Nov. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. ABE8e-N108Q

   Plasmid

   #194157

   Purpose

   Mammalian expression of ABE8e-N108Q

   Depositor

   Dali Li

   Article

   Chen et al Nat Chem Biol. 2022 Oct 13. pii: 10.1038

   Insert

   TadA-8e (N108Q)-nSpCas9
   

   Use

   CRISPR

   Tags

   6*His

   Expression

   Mammalian

   Available Since

   Jan. 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. Td-CGBE

   Plasmid

   #196599

   Purpose

   Mammalian expression of Td-CGBE

   Depositor

   Dali Li

   Article

   Chen et al Nat Biotechnol. 2022 Nov 10. doi: 10.103

   Insert

   bpNLS-TadA-8e (N46L)-32aa linker-nSpCas9-bpNLS
   

   Tags

   6*His

   Expression

   Mammalian

   Mutation

   TadA-8e (N46L); S. pyogenes Cas9 (D10A)

   Available Since

   March 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. AAV2-hSyn-G-Flamp1-mut

   Plasmid

   #188570

   Purpose

   control probe of G-Flamp1, insensitive to cAMP

   Depositor

   Jun Chu

   Article

   Wang et al Nat Commun. 2022 Sep 12;13(1):5363. doi:

   Insert

   G-Flamp1-mut
   

   Use

   AAV

   Tags

   His tag

   Promoter

   hSyn

   Available Since

   Oct. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. PA-Dre

   Plasmid

   #169706

   Purpose

   Photoactivatable Dre recombinase for AAV production and expression in mammalian cells

   Depositor

   Dali Li

   Article

   Li et al Proc Natl Acad Sci U S A. 2020 Dec 29;11

   Insert

   Dre246-nMag-T2A-pMag-Dre247
   

   Use

   AAV

   Expression

   Mammalian

   Mutation

   none

   Promoter

   CAG

   Available Since

   May 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. CALID

   Plasmid

   #169708

   Purpose

   Cre-activated light-inducible Dre recombinase

   Depositor

   Dali Li

   Article

   Li et al Proc Natl Acad Sci U S A. 2020 Dec 29;11

   Insert

   a double-floxed inverted Dre246-nMag-T2A-pMag-Dre247
   

   Expression

   Mammalian

   Mutation

   none

   Promoter

   CAG

   Available Since

   May 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. gNS2B47NS3 S135A

   Plasmid

   #155317

   Purpose

   DENV4 47 peptides from NS2B cofactor linked to NS3 full length by GGGGSGGGG linker S135A mutant

   Depositor

   Dahai Luo

   Article

   Phoo et al Antiviral Res. 2020 Oct;182:104900. doi:

   Insert

   DENV4 NS3 full length protein linked with 47 peptides from NS2B cofactor
   

   Tags

   His tag

   Expression

   Bacterial

   Mutation

   S135A

   Promoter

   M13

   Available Since

   Sept. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pRGEB32-CrU6-2-35S

    Plasmid

    #226706

    Purpose

    For CRISPR/Cas-mediated gene editing in model fern species, Ceratopteris richardii; expresses guide RNAs from CrU6-2 promoter and Cas9 from CaMV 35S promoter.

    Depositor

    Fenglin Deng

    Article

    Jiang et al Plant Physiol. 2024 Sep 12:kiae473. doi:

    Insert

    CrU6-2
    

    Use

    CRISPR

    Available Since

    Nov. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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