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We narrowed to 41,926 results for: tro

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Showing: 5641 - 5680 of 41926 results

  6. pEGB3 alpha1 35s:PIF6:tNos (GB0170)

    Plasmid
    #160606
    Purpose
    TU for the constitutive expression of a chimeric construct comprising a DNA binding protein fused to A. thaliana PIF6 (amino acids 1-100) and a nuclear localization sequence. Part of a red light-controlled synthetic genetic switch
    Depositor
    Diego Orzaez
    Article
    Calvache et al Plant Biotechnol J. 2022 Feb;20(2):399-4
    Insert
    PIF6
    Use
    Synthetic Biology
    Expression
    Plant
    Mutation
    BsaI and BsmBI sites removed
    Promoter
    P35S
    Available Since
    March 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    160606-plasmid-map-sequence-id-322045

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  19. PB-PE SpG_ATH51

    Plasmid
    #242072
    Purpose
    Expresses SpG_ATH51 prime editor and pegRNA in mammalian cells
    Depositor
    Tobias Cantz
    Article
    Eggenschwiler et al Nucleic Acids Res. 2025 Aug 11;53(15):gk
    Insert
    SpG_ATH51_H840A-PE2-MMLV-RT(dBB)-P2A-PAC_dTK(dBB)
    Use
    CRISPR and Synthetic Biology; Piggybac transposon
    Tags
    bpSV40 NLS
    Expression
    Mammalian
    Mutation
    ectopic insertion of 7 amino acids of PID turn-he…
    Available Since
    Oct. 29, 2025
    Availability
    Academic Institutions and Nonprofits only
    242072-plasmid-map-sequence-id-491030

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  24. pCS2+8-CYP2A6(C14A, C439A)-flag-tev-halo-his

    Plasmid
    #227162
    Purpose
    For T-REX experiments of CYP2A6 C14A and C439A double mutant. Less sensing ability to reactive electrophilic species (RES).
    Depositor
    Yimon Aye
    Article
    Liu et al Cell. 2024 Dec 26;187(26):7450-7469.e29.
    Insert
    CYP2A6 (CYP2A6 Human)
    Tags
    Flag, HaloTag, and His6
    Expression
    Mammalian
    Mutation
    changed cysteine 14 and cysteine 439 to alanine
    Promoter
    CMV and SP6
    Available Since
    Aug. 21, 2025
    Availability
    Academic Institutions and Nonprofits only
    227162-plasmid-map-sequence-id-459059

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  25. pCS2+8-cyp-33e1(C295A, C439A)-flag-tev-halo-his

    Plasmid
    #227158
    Purpose
    For T-REX experiments of cyp-33e1 C295A and C439A double mutant. Almost no sensing ability to reactive electrophilic species (RES).
    Depositor
    Yimon Aye
    Article
    Liu et al Cell. 2024 Dec 26;187(26):7450-7469.e29.
    Insert
    cyp-33e1 (cyp-33E1 Nematode)
    Tags
    Flag, HaloTag, and His6
    Expression
    Mammalian
    Mutation
    changed cysteine 295 and cysteine 439 to alanine
    Promoter
    CMV and SP6
    Available Since
    Aug. 19, 2025
    Availability
    Academic Institutions and Nonprofits only
    227158-plasmid-map-sequence-id-458998

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  27. pCS2+8-CYP2A6(C82A, C439A)-flag-tev-halo-his

    Plasmid
    #227163
    Purpose
    For T-REX experiments of CYP2A6 C82A and C439A double mutant. Almost no sensing ability to reactive electrophilic species (RES).
    Depositor
    Yimon Aye
    Article
    Liu et al Cell. 2024 Dec 26;187(26):7450-7469.e29.
    Insert
    CYP2A6 (CYP2A6 Human)
    Tags
    Flag, HaloTag, and His6
    Expression
    Mammalian
    Mutation
    changed cysteine 82 and cysteine 439 to alanine
    Promoter
    CMV and SP6
    Available Since
    Aug. 19, 2025
    Availability
    Academic Institutions and Nonprofits only
    227163-plasmid-map-sequence-id-459000

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  29. pGEMHE-MmCENPT-ASD2HFD-GFP

    Plasmid
    #237430
    Purpose
    Expresses Mus musculus CENP-T with ancestral HFD (mouse:rat); tagged with GFP at C-term; made for in vitro transcription (T7 promoter)
    Depositor
    Michael Lampson
    Article
    Dudka et al Curr Biol. 2025 Mar 10;35(5):1012-1022.e
    Insert
    Mus musculus CENP-T with ancestral HFD (Cenpt Mouse)
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    HFD (residues 413-503) swapped from mouse:rat anc…
    Promoter
    T7
    Available Since
    June 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    237430-plasmid-map-sequence-id-474256

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  30. pGEMHE-MmCENPT-RpHFD-GFP

    Plasmid
    #237428
    Purpose
    Expresses Mus musculus CENP-T with Rhabdomys pumilio HFD; tagged with GFP at C-term; made for in vitro transcription (T7 promoter)
    Depositor
    Michael Lampson
    Article
    Dudka et al Curr Biol. 2025 Mar 10;35(5):1012-1022.e
    Insert
    Mus musculus CENP-T with Rhabdomys pumilio HFD (Cenpt Mouse)
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    HFD (residues 413-503) swapped from Rhabdomys pum…
    Promoter
    T7
    Available Since
    June 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    237428-plasmid-map-sequence-id-474254

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  31. pGEMHE-MmCENPT-McHFD-GFP

    Plasmid
    #237437
    Purpose
    Expresses Mus musculus CENP-T with Mus caroli HFD; tagged with GFP at C-term; made for in vitro transcription (T7 promoter)
    Depositor
    Michael Lampson
    Article
    Dudka et al Curr Biol. 2025 Mar 10;35(5):1012-1022.e
    Insert
    Mus musculus CENP-T with Mus caroli HFD (Cenpt Mouse)
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    HFD (residues 413-503) swapped from Mus caroli
    Promoter
    T7
    Available Since
    June 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    237437-plasmid-map-sequence-id-474253

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  32. pGEMHE-MmCENPT-RnHFD-GFP

    Plasmid
    #237429
    Purpose
    Expresses Mus musculus CENP-T with Rattus norvegicus HFD; tagged with GFP at C-term; made for in vitro transcription (T7 promoter)
    Depositor
    Michael Lampson
    Article
    Dudka et al Curr Biol. 2025 Mar 10;35(5):1012-1022.e
    Insert
    Mus musculus CENP-T with Rattus norvegicus HFD (Cenpt Mouse)
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    HFD (residues 413-503) swapped from Rattus norveg…
    Promoter
    T7
    Available Since
    June 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    237429-plasmid-map-sequence-id-474225

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  33. pGEMHE-MmCENPT-MsHFD-GFP

    Plasmid
    #237427
    Purpose
    Expresses Mus musculus CENP-T with Mus spretus HFD; tagged with GFP at C-term; made for in vitro transcription (T7 promoter)
    Depositor
    Michael Lampson
    Article
    Dudka et al Curr Biol. 2025 Mar 10;35(5):1012-1022.e
    Insert
    Mus musculus CENP-T with Mus spretus HFD (Cenpt Mouse)
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    HFD (residues 413-503) swapped from Mus spretus
    Promoter
    T7
    Available Since
    June 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    237427-plasmid-map-sequence-id-474221

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  34. pdnCas3-CDA1

    Plasmid
    #229535
    Purpose
    pMV_hyg encoding dnCas3(H74A+D75A)-CDA1 fusion construct and crRNA expression cassette. Respective crRNA spacer can be introduced via BsmBI restriction cloning
    Depositor
    Kevin Verstrepen
    Article
    Zimmermann et al Nat Commun. 2023 Jun 9;14(1):3389. doi:
    Inserts
    dnCas3
    Cytidine deaminase
    Uracil glycosylase inhibitor
    Tags
    XTEN
    Expression
    Bacterial and Yeast
    Mutation
    codon optimized for S.cerevisiae and codon optimi…
    Available Since
    Jan. 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    229535-plasmid-map-sequence-id-459013

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  37. AAV-CMV-TetRC-2A-Tomato-bGHpA;U6_BsaI-sgRNA

    Plasmid
    #177365
    Purpose
    AAV expression of Chimeric guide for SaCas9 and reverse tetracycline-controlled transactivator for doxycycline controlled expression
    Depositor
    Naoki Yamamoto
    Article
    Yamamoto et al Research Square [Preprint] 2021. doi: 10
    Inserts
    tdTomato
    Chimeric guide for SaCas9
    Use
    AAV
    Tags
    P2A and a tetracycline-controlled transactivator …
    Expression
    Mammalian
    Promoter
    CMV and U6 promoter
    Available Since
    Sept. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    177365-plasmid-map-sequence-id-353664

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Showing: 5641 - 5680 of 41926 results