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memb-mCherry-pA

Plasmid

#241157

Purpose

Middle entry vector for mini-Golden subcloning platform; mCherry

Depositor

Junsu Kang

Article

Rodriguez-Parks et al Biol Open. 2026 Feb 2:bio.062472. doi: 1

Insert

mCherry

Use

Mini-golden

Mutation

TCGC linker

Available Since

Feb. 10, 2026

Availability

Academic Institutions and Nonprofits only

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memb-BFP-pA

Plasmid

#241161

Purpose

Middle entry vector for mini-Golden subcloning platform; BFP

Depositor

Junsu Kang

Article

Rodriguez-Parks et al Biol Open. 2026 Feb 2:bio.062472. doi: 1

Insert

BFP

Use

Mini-golden

Mutation

TCGC linker

Available Since

Feb. 10, 2026

Availability

Academic Institutions and Nonprofits only

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pTE5609

Plasmid

#250515

Purpose

Cell-free translocation reporter; proOmpA (1-178); C-terminal pep86 tag

Depositor

Tobias Erb

Article

Meier et al bioRxiv 2025.12.09.688994

Insert

T7-proOmpA-pep86 (ompA E. coli)

Tags

pep86

Expression

Bacterial

Promoter

T7

Available Since

Feb. 5, 2026

Availability

Academic Institutions and Nonprofits only

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pTE5602

Plasmid

#250510

Purpose

Cell-free translocation reporter; proOmpA (1-178); C-terminal pep99 tag

Depositor

Tobias Erb

Article

Meier et al bioRxiv 2025.12.09.688994

Insert

T7-proOmpA-pep99 (ompA E. coli)

Tags

pep99

Expression

Bacterial

Promoter

T7

Available Since

Feb. 5, 2026

Availability

Academic Institutions and Nonprofits only

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pTE5603

Plasmid

#250511

Purpose

Cell-free membrane insertion reporter; proteorhodopsin (UniProt ID: Q9F7P4; C-terminal transmembrane domain deleted); C-terminal pep104 tag

Depositor

Tobias Erb

Article

Meier et al bioRxiv 2025.12.09.688994

Insert

T7-PR(-1tm)-pep104

Tags

pep104

Expression

Bacterial

Promoter

T7

Available Since

Feb. 4, 2026

Availability

Academic Institutions and Nonprofits only

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pTE5629

Plasmid

#250517

Purpose

Cell-free translocation reporter; translocation of a nanobody; N-terminal YncJ signal peptide; C-terminal pep86 tag

Depositor

Tobias Erb

Article

Meier et al bioRxiv 2025.12.09.688994

Insert

T7-pYncJss-nanobody-HiBiT

Tags

pep86

Expression

Bacterial

Promoter

T7

Available Since

Feb. 4, 2026

Availability

Academic Institutions and Nonprofits only

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pHREP-KD-2-shH2B

Plasmid

#241996

Purpose

Sleeping Beauty vector for inducible expression of a chicken H2B targeting shRNA using a shortened mir30 sequence. Allows co-selection with Puromycin.

Depositor

Lienhard Schmitz

Article

Pfisterer et al PLoS One. 2026 Feb 10;21(2):e0342014. do

Insert

H2B

Expression

Mammalian

Available Since

Feb. 4, 2026

Availability

Academic Institutions and Nonprofits only

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pHREP-KD-2-shH2A

Plasmid

#241997

Purpose

Sleeping Beauty vector for inducible expression of a chicken H2A targeting shRNA using a shortened mir30 sequence. Allows co-selection with Puromycin.

Depositor

Lienhard Schmitz

Article

Pfisterer et al PLoS One. 2026 Feb 10;21(2):e0342014. do

Insert

H2A

Expression

Mammalian

Available Since

Feb. 4, 2026

Availability

Academic Institutions and Nonprofits only

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pHREP-KD-1-shH2B

Plasmid

#240417

Purpose

Sleeping Beauty vector for inducible expression of a chicken H2B targeting shRNA using a shortened mir30 sequence. Allows co-selection with Puromycin.

Depositor

Lienhard Schmitz

Article

Pfisterer et al PLoS One. 2026 Feb 10;21(2):e0342014. do

Insert

H2B

Expression

Mammalian

Promoter

TRE

Available Since

Feb. 4, 2026

Availability

Academic Institutions and Nonprofits only

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pHREP-KD-2-shCtrl

Plasmid

#240580

Purpose

Sleeping Beauty vector for inducible expression of a unspecific control shRNA using a shortened mir30 sequence. Allows co-selection with Puromycin.

Depositor

Lienhard Schmitz

Article

Pfisterer et al PLoS One. 2026 Feb 10;21(2):e0342014. do

Insert

Scramble shRNA

Expression

Mammalian

Available Since

Feb. 4, 2026

Availability

Academic Institutions and Nonprofits only

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PiggyBac_8mer_pegArray_bb_pos.1

Plasmid

#238542

Purpose

Golden gate acceptor backbone for position 1 8-mer pegArrays

Depositor

Jonathan Weissman

Article

Koblan et al Science. 2025 Jul 24:eadx3800. doi: 10.1

Type

Empty backbone

Expression

Mammalian

Available Since

Feb. 2, 2026

Availability

Academic Institutions and Nonprofits only

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PiggyBac_8mer_pegArray_bb_pos.2

Plasmid

#238543

Purpose

Golden gate acceptor backbone for position 2 8-mer pegArrays

Depositor

Jonathan Weissman

Article

Koblan et al Science. 2025 Jul 24:eadx3800. doi: 10.1

Type

Empty backbone

Expression

Mammalian

Available Since

Feb. 2, 2026

Availability

Academic Institutions and Nonprofits only

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PiggyBac_8mer_pegArray_bb_pos.3

Plasmid

#238544

Purpose

Golden gate acceptor backbone for position 3 8-mer pegArrays

Depositor

Jonathan Weissman

Article

Koblan et al Science. 2025 Jul 24:eadx3800. doi: 10.1

Type

Empty backbone

Expression

Mammalian

Available Since

Feb. 2, 2026

Availability

Academic Institutions and Nonprofits only

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pCK161.06

Plasmid

#242925

Purpose

Reporter J1-BBa_J23117-mRFP with expression of MS2 scRNA J106

Depositor

Jesse Zalatan

Article

Kiattisewee et al ACS Synth Biol. 2025 Jul 22. doi: 10.102

Insert

scRNA MS2 J106

Use

CRISPR and Synthetic Biology

Promoter

Bba_J23119

Available Since

Jan. 22, 2026

Availability

Academic Institutions and Nonprofits only

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pG20_3C_mVenus_FR

Plasmid

#240669

Purpose

Expression plasmid for 3C-mVenus-tagged proteins with FAST-Red transgenic seed selection

Depositor

Hans-Henning Kunz

Article

Schwartz et al Plant J. 2025 Nov;124(3):e70544. doi: 10

Type

Empty backbone

Tags

3C-mVenus

Expression

Plant

Available Since

Nov. 12, 2025

Availability

Academic Institutions and Nonprofits only

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pG20_FG

Plasmid

#240670

Purpose

Expression plasmid for un-tagged proteins with FAST-Green transgenic seed selection

Depositor

Hans-Henning Kunz

Article

Schwartz et al Plant J. 2025 Nov;124(3):e70544. doi: 10

Type

Empty backbone

Expression

Plant

Available Since

Nov. 12, 2025

Availability

Academic Institutions and Nonprofits only

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pG20_TEV_HA_FG

Plasmid

#240677

Purpose

Expression plasmid for TEV-HA-tagged proteins with FAST-Green transgenic seed selection

Depositor

Hans-Henning Kunz

Article

Schwartz et al Plant J. 2025 Nov;124(3):e70544. doi: 10

Type

Empty backbone

Tags

TEV-HA

Expression

Plant

Available Since

Nov. 12, 2025

Availability

Academic Institutions and Nonprofits only

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pG20_3C_mVenus_FG

Plasmid

#240679

Purpose

Expression plasmid for 3C-mVenus-tagged proteins with FAST-Green transgenic seed selection

Depositor

Hans-Henning Kunz

Article

Schwartz et al Plant J. 2025 Nov;124(3):e70544. doi: 10

Type

Empty backbone

Tags

3C-mVenus

Expression

Plant

Available Since

Nov. 12, 2025

Availability

Academic Institutions and Nonprofits only

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pG20_TEV_mCherry_Hyg

Plasmid

#240652

Purpose

Expression plasmid for TEV-mCherry-tagged proteins with Hygromycin transgenic seed selection

Depositor

Hans-Henning Kunz

Article

Schwartz et al Plant J. 2025 Nov;124(3):e70544. doi: 10

Type

Empty backbone

Tags

TEV-mCherry

Expression

Plant

Available Since

Nov. 12, 2025

Availability

Academic Institutions and Nonprofits only

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pG20_TEV_2xStrepII_Hyg

Plasmid

#240655

Purpose

Expression plasmid for TEV-2xStrepII-tagged proteins with Hygromycin transgenic seed selection

Depositor

Hans-Henning Kunz

Article

Schwartz et al Plant J. 2025 Nov;124(3):e70544. doi: 10

Type

Empty backbone

Tags

TEV-2xStrepII

Expression

Plant

Available Since

Nov. 12, 2025

Availability

Academic Institutions and Nonprofits only

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