We narrowed to 10,981 results for: Vars;

1. pcDNA3-MAVS-M142A(KaganF59)

   Plasmid

   #52062

   Purpose

   Expresses the human MAVS CDS containing the point mutation M142A "weak translational context"

   Depositor

   Jonathan Kagan

   Article

   Brubaker et al Cell. 2014 Feb 13;156(4):800-11. doi: 10

   Insert

   MAVS-M142A
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   changed Met 142 to Ala

   Promoter

   Available since

   March 24, 2014

   Availability

   Academic Institutions and Nonprofits only

2. pMSCV-MAVS-M1A,M142A(KaganE20)

   Plasmid

   #52011

   Purpose

   Expresses the human MAVS CDS containing the point mutations M1A and M142A

   Depositor

   Jonathan Kagan

   Article

   Brubaker et al Cell. 2014 Feb 13;156(4):800-11. doi: 10

   Insert

   MAVS-M1A,M142A
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   changed Met 1 to Ala and Met 142 to Ala

   Promoter

   Available since

   March 24, 2014

   Availability

   Academic Institutions and Nonprofits only

3. pFUS2_b3

   Plasmid

   #50697

   Purpose

   Intermediate array vector

   Depositor

   Takashi Yamamoto

   Article

   Sakuma et al Sci Rep. 2013 Nov 29;3:3379. doi: 10.103

   Insert

   LacZ + BsaI restriction sites
   

   Use

   TALEN

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 3, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pFUS2_b2

   Plasmid

   #50696

   Purpose

   Intermediate array vector

   Depositor

   Takashi Yamamoto

   Article

   Sakuma et al Sci Rep. 2013 Nov 29;3:3379. doi: 10.103

   Insert

   LacZ + BsaI restriction sites
   

   Use

   TALEN

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 3, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. p3HD

   Plasmid

   #50672

   Purpose

   Contains third TALE repeat harboring HD as RVD

   Depositor

   Takashi Yamamoto

   Article

   Sakuma et al Sci Rep. 2013 Nov 29;3:3379. doi: 10.103

   Insert

   repeat 3HD
   

   Use

   TALEN

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 10, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. γA3/N-GFP

   Plasmid

   #33113

   Depositor

   Greg Phillips

   Article

   O'Leary et al Mol Biol Cell. 2011 Sep 14.

   Insert

   Pcdh-γA3 (Pcdhga3 Mouse)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Cytoplasmic domain has been replaced with the N-c…

   Promoter

   CMV

   Available since

   Oct. 15, 2012

   Availability

   Academic Institutions and Nonprofits only

7. Δ741-752Δconst-GFP

   Plasmid

   #33120

   Depositor

   Greg Phillips

   Article

   O'Leary et al Mol Biol Cell. 2011 Sep 14.

   Insert

   Pcdh-γA3 (Pcdhga3 Mouse)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Deletion of aa 741-752 along with the entire cons…

   Promoter

   CMV

   Available since

   Oct. 15, 2012

   Availability

   Academic Institutions and Nonprofits only

8. pET-sCS4T

   Plasmid

   #28249

   Depositor

   Yawen Bai

   Article

   Zhou et al Nature. 2011 Apr 14. 472(7342):234-7.

   Insert

   single chain Cse4-Scm3-H4 with Thrombin site
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   A thrombin cutting site between Cse4 and Scm3

   Promoter

   Available since

   July 12, 2011

   Availability

   Academic Institutions and Nonprofits only

9. pET-sCS4T+N

   Plasmid

   #28255

   Depositor

   Yawen Bai

   Article

   Zhou et al Nature. 2011 Apr 14. 472(7342):234-7.

   Insert

   sCS4T+N
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   single chain CSE4-SCM3-H4 with Thrombin site, SCM…

   Promoter

   Available since

   July 12, 2011

   Availability

   Academic Institutions and Nonprofits only

10. pET-sC4T

    Plasmid

    #28251

    Depositor

    Yawen Bai

    Article

    Zhou et al Nature. 2011 Apr 14. 472(7342):234-7.

    Insert

    single chain Cse4-H4 with Thrombin site
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    A thrombin cutting site between Cse4 and H4

    Promoter

    Available since

    July 11, 2011

    Availability

    Academic Institutions and Nonprofits only

11. pV1338

    Plasmid

    #111443

    Purpose

    Solo vector pV1326 + sgScADE2

    Depositor

    Gerald Fink

    Article

    Vyas et al mSphere. 2018 Apr 25;3(2). pii: 3/2/e001

    Insert

    CaCas9/sgScADE2
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET15b-RvLEAMshort

    Plasmid

    #218122

    Purpose

    Expresses truncated RvLEAM protein under IPTG induction in bacterial host cells.

    Depositor

    Ci Ji Lim

    Article

    Abe et al bioRxiv [Preprint]. 2024 Feb 11:2024.02.

    Insert

    RvLEAMshort
    

    Use

    Tags

    6xHIS

    Expression

    Bacterial

    Mutation

    Truncated form (58-181aa)

    Promoter

    T7

    Available since

    April 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. p21-Reporter

    Plasmid

    #52432

    Purpose

    DNA damage inducible Red Fluorescent Protein for mammalian expression

    Depositor

    Joel Voldman

    Article

    Fendyur et al Anal Chem. 2014 Aug 5;86(15):7598-605. d

    Insert

    p21-p53 Tandem Response Element Promoter
    

    Use

    Tags

    TurboRFP

    Expression

    Mammalian

    Mutation

    Promoter

    13X repeated p21-p53 response element

    Available since

    Oct. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA5/FRT/TO_Lifeact-HaloTag11-T2A-Tomm20-HaloTag7

    Plasmid

    #175526

    Purpose

    Co-expression of HaloTag11 (actin) and 7 (mitochondrial surface) in mammalian cells

    Depositor

    Kai Johnsson

    Article

    Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

    Insert

    Lifeact-HaloTag11 and Tomm20-HaloTag7
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    HaloTag11 = HaloTag7-M175W

    Promoter

    Available since

    Dec. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pRSETB/mStayGold

    Plasmid

    #212017

    Purpose

    mStayGold has an n1 adaptor at its N-terminus and is useful for C-terminal tagging. This construct is used also for expression of mStayGold itself.

    Depositor

    Atsushi Miyawaki

    Article

    Ando et al Nat Methods. 2023 Nov 30. doi: 10.1038/s

    Insert

    mStayGold
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Jan. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. ABE8r

    Plasmid

    #208294

    Purpose

    Expresses ABE8r in mammalian cells

    Depositor

    Weixin Tang

    Article

    Xiao et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

    Insert

    TadA8r-SpCas9 D10A
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    W23R, H36L, R47K, P48A, R51L, I76Y, V82S, A106V, …

    Promoter

    Available since

    Jan. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pK18sB

    Plasmid

    #177838

    Purpose

    Plasmid for gene replacement using kanamycin/sucrose selection/counterselection. Derived from pK18mobsacB but reduced in size and lacking oriT required for conjugation.

    Depositor

    Christopher Johnson, Gregg Beckham

    Article

    Jayakody et al Energy Environ. Sci., 2018,11, 1625-1638

    Type

    Empty backbone

    Use

    Bacterial gene replacement

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. VRQR-ABEmax

    Plasmid

    #119811

    Purpose

    A-to-G base editor

    Depositor

    David Liu

    Article

    Huang et al Nat Biotechnol. 2019 May 20. pii: 10.103

    Insert

    ecTadA-ecTadA*(7.10)-nSpCas9 (VRQR)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pRK5 beta1

    Plasmid

    #16042

    Depositor

    Filippo Giancotti

    Article

    Giancotti et al Cell. 1990 Mar 9. 60(5):849-59.

    Insert

    beta1 (ITGB1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 2, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. UBC plasmid

    Plasmid

    #169746

    Purpose

    Unregulated "open-loop" UBC promoter plasmid. This plasmid encodes eGFP to report circuit output levels.

    Depositor

    Francois St-Pierre

    Article

    Yang et al Nat Commun. 2021 Jul 5;12(1):4132. doi:

    Insert

    EGFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    UBC

    Available since

    Oct. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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