We narrowed to 9,260 results for: pho

1. pT7-7 asyn E46K

   Plasmid

   #105728

   Purpose

   Bacterial expression of mutant human alpha synuclein

   Depositor

   Hilal Lashuel

   Article

   Mbefo et al J Biol Chem. 2015 Apr 10;290(15):9412-27

   Insert

   alpha-synuclein E46K (SNCA Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   E46K

   Promoter

   T7

   Available since

   April 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1/Myc-His beta4

   Plasmid

   #16039

   Depositor

   Filippo Giancotti

   Article

   Dans et al J Biol Chem. 2001 Jan 12. 276(2):1494-50

   Insert

   beta4 (ITGB4 Human)
   

   Use

   Tags

   His and Myc

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 2, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET28a-SET

   Plasmid

   #234708

   Purpose

   expresses His-tagged human SET in E coli

   Depositor

   Lienhard Schmitz

   Article

   Seibert et al J Cell Biol. 2019 Apr 1;218(4):1164-1181

   Insert

   SET (SET Human)
   

   Use

   Tags

   (His)6

   Expression

   Bacterial

   Mutation

   Promoter

   lacI

   Available since

   April 23, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCMV-hARdelta120-472

   Plasmid

   #89088

   Purpose

   mammalian expression of human androgen receptor with deletion of 120-472 amino acid residues

   Depositor

   Elizabeth Wilson

   Article

   Bai et al Mol Cell Biol. 2008 Mar;28(6):1947-63. d

   Insert

   hAR-120-472 N-term deletion (AR Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   hAR-120-472

   Promoter

   CMV

   Available since

   April 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA6 asyn A30P

   Plasmid

   #105729

   Purpose

   Mammalian expression of mutant human alpha synuclein

   Depositor

   Hilal Lashuel

   Article

   Mbefo et al J Biol Chem. 2015 Apr 10;290(15):9412-27

   Insert

   alpha-synuclein A30P (SNCA Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   A30P

   Promoter

   CMV

   Available since

   March 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pFRT-FLAG-HA-(N)-CDK12

   Plasmid

   #231750

   Purpose

   Expression in Flp-IN human cells

   Depositor

   Jesper Svejstrup

   Article

   Lopez Martinez et al Mol Cell. 2025 May 15;85(10):1952-1967.e

   Insert

   CDK12 (CDK12 Human)
   

   Use

   Tags

   Flag-HA-3C

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3.1-Flag-mRIPK1-S321E

   Plasmid

   #115340

   Purpose

   Mammalian expression of Flag-mRIPK1-S321E

   Depositor

   Junying Yuan

   Article

   Geng et al Nat Commun. 2017 Aug 25;8(1):359. doi: 1

   Insert

   RIPK1 (Ripk1 Mouse)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   S321E

   Promoter

   Available since

   Sept. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. GFP-PH-TAPP1

   Plasmid

   #161985

   Purpose

   Expresses GFP-tagged PH-Tapp1 in mammalian cells (PI(3,4)P2 biosensor)

   Depositor

   Dario Alessi, David Bryant

   Article

   Posor et al Nature. 2013 Jul 11;499(7457):233-7. doi

   Insert

   PH-TAPP1 (PLEKHA1 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCAGImC_V5-CSNK1A1

   Plasmid

   #92014

   Purpose

   Expresses V5-CSNK1A1, includes IRES-mCherry

   Depositor

   Joshua Mendell

   Article

   Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

   Insert

   CSNK1A1 (CSNK1A1 Human)
   

   Use

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pLJM1-FH-AGO2-WT

    Plasmid

    #91978

    Purpose

    Expresses FLAG-HA-AGO2 (WT)

    Depositor

    Joshua Mendell

    Article

    Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

    Insert

    AGO2 (AGO2 Human)
    

    Use

    Lentiviral

    Tags

    FLAG-HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. mEGFP-PI35P2

    Plasmid

    #92419

    Purpose

    Fluorescent reporter for phosphatidylinositol (3,5)-bisphosphate (PI(3,5)P2). Mouse MCOLN1 residues 1–68 fused to eGFP.

    Depositor

    Geert van den Bogaart

    Article

    Baranov et al Small GTPases. 2017 May 10:0. doi: 10.10

    Insert

    MCOLN1 (Mcoln1 Mouse)
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Synthetic gene, codon optimized for human.

    Promoter

    CMV

    Available since

    July 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. dCas9-ddMSK1

    Plasmid

    #165603

    Purpose

    Expresses Sp dCas9 fused to truncated inactive human MSK1 (42-802, D195A, D565A)

    Depositor

    Isaac Hilton

    Article

    Li et al Nat Commun. 2021 Feb 9;12(1):896. doi: 1

    Insert

    S. Pyogenes dCas9 with c-terminal truncated inactive human Mitogen- and stress-activated protein kinase-1 (42-802, D195A, D565A) (RPS6KA5 Human, S. Pyogenes, Synthetic)
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG Tag

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    March 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. GFP-DroshaS300/302A

    Plasmid

    #62530

    Purpose

    Serines 300 and 302 of Drosha are mutated to alanines

    Depositor

    Bharat Ramratnam

    Article

    Tang et al Nucleic Acids Res. 2010 Oct;38(19):6610-

    Insert

    human Drosha with serines 300 and 302 changed alanines (DROSHA Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    changed serines 300 and 302 to alanines

    Promoter

    Available since

    March 20, 2015

    Availability

    Academic Institutions and Nonprofits only

14. pclbw-opa1(isoform 1)-myc

    Plasmid

    #62845

    Purpose

    Mammalian Expression of myc-tagged Opa1 isoform1

    Depositor

    David Chan

    Article

    Mishra et al Cell Metab. 2014 Apr 1;19(4):630-41. doi

    Insert

    mmOPA1isoform1-myc (Opa1 Mouse)
    

    Use

    Retroviral

    Tags

    9xMyc

    Expression

    Mammalian

    Mutation

    Promoter

    cmv-bactin

    Available since

    Jan. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

15. pGEX Src-SH2

    Plasmid

    #46510

    Depositor

    Bruce Mayer

    Article

    Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

    Insert

    Src (SRC Human)
    

    Use

    Tags

    GST and PreScission

    Expression

    Bacterial

    Mutation

    contains amino acids 142-252

    Promoter

    Tac

    Available since

    July 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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16. hHSF1 S303/7A

    Plasmid

    #118365

    Purpose

    This plasmid expresses human HSF1 S303A and S307A mutant, which cannot undergo sumoylation on K298 due to disrupted PDSM motif.

    Depositor

    Lea Sistonen

    Article

    Hietakangas et al Mol Cell Biol. 2003 Apr . 23(8):2953-68.

    Insert

    hHSF1 (HSF1 Human)
    

    Use

    Tags

    myc and 6xHIS

    Expression

    Mammalian

    Mutation

    S303A and S307A

    Promoter

    Available since

    Nov. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. SYKA-c020

    Plasmid

    #175489

    Purpose

    Protein expression in bacterial cells. Tandem SH2 domains, M6-N269. Can be used for crystallography.

    Depositor

    Opher Gileadi

    Article

    Bashore et al bioRxiv. 2023 Jul 29:2023.07.28.551026.

    Insert

    SYK (SYK Human)
    

    Use

    Tags

    His6-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pRecLV105

    Plasmid

    #107238

    Purpose

    A lentiviral vector for expression in mammalian cells

    Depositor

    Alex Kentsis

    Article

    Brown et al Cancer Discov. 2018 Apr;8(4):478-497. do

    Type

    Empty backbone

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNA 3.2/V5-DEST MTFR1L-V5

    Plasmid

    #69822

    Purpose

    Expresses MTFR1L-V5 in mammalian cells

    Depositor

    Anne Brunet

    Article

    Schaffer et al Cell Metab. 2015 Nov 3;22(5):907-21. doi

    Insert

    Mitochondrial fission regulator 1-like (MTFR1L Human)
    

    Use

    Retroviral

    Tags

    v5

    Expression

    Mammalian

    Mutation

    Promoter

    cmv

    Available since

    April 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pGEX FynA-SH2

    Plasmid

    #46429

    Depositor

    Bruce Mayer

    Article

    Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

    Insert

    FynA (FYN Human)
    

    Use

    Tags

    AviTag, GST, and PreScission

    Expression

    Bacterial

    Mutation

    contains amino acids 142-251

    Promoter

    Tac

    Available since

    July 12, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge