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pTrcHis_VectorA_short_speGpromoter_PA1472

Plasmid

#220475

Purpose

heterologous expression of PA1472 under endogenous speG E. coli promoter

Depositor

Emily Balskus

Article

Mayers et al Cell. 2024 Jun 11:S0092-8674(24)00579-8.

Insert

PA1472

Expression

Bacterial

Mutation

none

Available Since

June 26, 2024

Availability

Academic Institutions and Nonprofits only

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pTrcHis_VectorA_short_speGpromoter_PA1377

Plasmid

#220476

Purpose

heterologous expression of PA1377 under endogenous speG E. coli promoter

Depositor

Emily Balskus

Article

Mayers et al Cell. 2024 Jun 11:S0092-8674(24)00579-8.

Insert

PA1377

Expression

Bacterial

Mutation

none

Available Since

June 26, 2024

Availability

Academic Institutions and Nonprofits only

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TRE-tdTomato

Plasmid

#210512

Purpose

tdTomato reporter vector for LAUNCHER system

Depositor

Dongmin Lee

Article

Cui et al Nat Chem Biol. 2024 Mar;20(3):353-364. d

Insert

tdTomato

Expression

Mammalian

Promoter

TetO

Available Since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pGEX SYT9 C2A

Plasmid

#195707

Purpose

Bacterial expression plasmid encoding GST-tagged SYT9 C2A domain

Depositor

Edwin Chapman

Article

Seibert et al J Neurosci. 2023 Mar 1;43(9):1475-1491.

Insert

Syt9 (Syt5 Mouse)

Tags

GST

Expression

Bacterial

Mutation

encodes amino acids 124-211 of mouse SYT9

Available Since

Oct. 16, 2023

Availability

Academic Institutions and Nonprofits only

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pGEX SYT9 C2B

Plasmid

#195708

Purpose

Bacterial expression plasmid encoding GST-tagged SYT9 C2B domain

Depositor

Edwin Chapman

Article

Seibert et al J Neurosci. 2023 Mar 1;43(9):1475-1491.

Insert

Syt9 (Syt5 Mouse)

Tags

GST

Expression

Bacterial

Mutation

encodes amino acids 253-344 of mouse SYT9

Available Since

Oct. 16, 2023

Availability

Academic Institutions and Nonprofits only

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pGEX SYT9 C2AB

Plasmid

#195709

Purpose

Bacterial expression plasmid encoding GST-tagged SYT9 C2AB domain

Depositor

Edwin Chapman

Article

Seibert et al J Neurosci. 2023 Mar 1;43(9):1475-1491.

Insert

Syt9 (Syt5 Mouse)

Tags

GST

Expression

Bacterial

Mutation

encodes amino acids 104-386 of mouse SYT9

Available Since

Oct. 16, 2023

Availability

Academic Institutions and Nonprofits only

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pGEX SYT1 C2A

Plasmid

#195710

Purpose

Bacterial expression plasmid encoding GST-tagged SYT1 C2A domain

Depositor

Edwin Chapman

Article

Seibert et al J Neurosci. 2023 Mar 1;43(9):1475-1491.

Insert

Syt1 (Syt1 Rat)

Tags

GST

Expression

Bacterial

Mutation

encodes amino acids 96-264 of mouse SYT1

Available Since

Oct. 16, 2023

Availability

Academic Institutions and Nonprofits only

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pGEX SYT1 C2B

Plasmid

#195711

Purpose

Bacterial expression plasmid encoding GST-tagged SYT1 C2B domain

Depositor

Edwin Chapman

Article

Seibert et al J Neurosci. 2023 Mar 1;43(9):1475-1491.

Insert

Syt1 (Syt1 Rat)

Tags

GST

Expression

Bacterial

Mutation

encodes amino acids 248-421 of mouse SYT1

Available Since

Oct. 16, 2023

Availability

Academic Institutions and Nonprofits only

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pGEX SYT1 C2AB

Plasmid

#195712

Purpose

Bacterial expression plasmid encoding GST-tagged SYT1 C2AB domain

Depositor

Edwin Chapman

Article

Seibert et al J Neurosci. 2023 Mar 1;43(9):1475-1491.

Insert

Syt1 (Syt1 Rat)

Tags

GST

Expression

Bacterial

Mutation

encodes amino acids 96-421 of mouse SYT1

Available Since

Oct. 16, 2023

Availability

Academic Institutions and Nonprofits only

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pET (SUMO)-Syt9

Plasmid

#195713

Purpose

Bacterial expression plasmid encoding SUMO-tagged full-length Syt9

Depositor

Edwin Chapman

Article

Seibert et al J Neurosci. 2023 Mar 1;43(9):1475-1491.

Insert

Syt9 (Syt5 Rat)

Tags

GST-SUMO

Expression

Bacterial

Available Since

Oct. 16, 2023

Availability

Academic Institutions and Nonprofits only

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H3-pAc5.1 OST4 mCherry Oskar 139-606

Plasmid

#206494

Purpose

Oskar 139-606 expression for Schneider cell imaging

Depositor

Anne Ephrussi

Article

Salgania et al Nat Commun. 2024 Apr 3;15(1):2875. doi:

Insert

Oskar 139-606

Tags

OST4 mcherry

Expression

Insect

Available Since

Oct. 3, 2023

Availability

Academic Institutions and Nonprofits only

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F19-pAc5.1 EGFP Vasa 463-661

Plasmid

#206486

Purpose

Vasa 463-661 expression for Schneider cell imaging

Depositor

Anne Ephrussi

Article

Salgania et al Nat Commun. 2024 Apr 3;15(1):2875. doi:

Insert

Vasa 463-661

Tags

EGFP

Expression

Insect

Available Since

Sept. 12, 2023

Availability

Academic Institutions and Nonprofits only

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F18-pAc5.1 EGFP Vasa 200-462

Plasmid

#206485

Purpose

Vasa 200-642 expression for Schneider cell imaging

Depositor

Anne Ephrussi

Article

Salgania et al Nat Commun. 2024 Apr 3;15(1):2875. doi:

Insert

Vasa 200-462

Tags

EGFP

Expression

Insect

Available Since

Sept. 12, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmNot4_F

Plasmid

#146275

Purpose

Insect Expression of DmNot4

Depositor

Elisa Izaurralde

Article

Zekri et al Mol Cell Biol. 2009 Dec;29(23):6220-31.

Insert

DmNot4 (CG31716 Fly)

Expression

Insect

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1A-DmNot4-EGFP_F

Plasmid

#146273

Purpose

Insect Expression of DmNot4

Depositor

Elisa Izaurralde

Article

Zekri et al Mol Cell Biol. 2009 Dec;29(23):6220-31.

Insert

DmNot4 (CG31716 Fly)

Expression

Insect

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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eSpCas9-EF

Plasmid

#177267

Purpose

CRISPR vector

Depositor

Hiroshi Ochiai

Article

Ohishi et al Nat Commun. 2022 Dec 20;13(1):7672. doi:

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Available Since

Dec. 22, 2022

Availability

Academic Institutions and Nonprofits only

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CROP_C9_Puro_MTOR

Plasmid

#183311

Purpose

All-in-One CRISPRko system with a guide RNA that targets MTOR gene

Depositor

Prashant Mali

Article

Hu et al Biomaterials. 2022 Jan;280:121276. doi:

Insert

MTOR

Use

Lentiviral

Available Since

July 19, 2022

Availability

Academic Institutions and Nonprofits only

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CROP_C9_Puro_BRAF

Plasmid

#183273

Purpose

All-in-One CRISPRko system with a guide RNA that targets BRAF gene

Depositor

Prashant Mali

Article

Hu et al Biomaterials. 2022 Jan;280:121276. doi:

Insert

BRAF

Use

Lentiviral

Available Since

July 19, 2022

Availability

Academic Institutions and Nonprofits only

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pLV-PGK-SNAP-MD-OCT4

Plasmid

#115691

Purpose

Constitutive expression of an OCT4 (Pou5f1) protein tagged with SNAP-tag and a mitotic degron (MD)

Depositor

David Suter

Article

Friman et al eLife. 2019 Dec 3;8. pii: 50087. doi: 10

Insert

SNAP-MD-OCT4

Use

Lentiviral

Available Since

July 6, 2022

Availability

Academic Institutions and Nonprofits only

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CROP_C9_Puro_CYP19A1

Plasmid

#183280

Purpose

All-in-One CRISPRko system with a guide RNA that targets CYP19A1 gene

Depositor

Prashant Mali

Article

Hu et al Biomaterials. 2022 Jan;280:121276. doi:

Insert

CYP19A1

Use

Lentiviral

Available Since

June 16, 2022

Availability

Academic Institutions and Nonprofits only

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