We narrowed to 29,613 results for: des.2
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Plasmid#61256PurposeCRISPR-RMCE, second step attB plasmid, intron phase 1, exchange dsRed with 2xTY1 and V5 tags cassetteDepositorInsert2xTY1-V5
UseCRISPRTagsTY1 and V5Available SinceNov. 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
EW425 CMV-TO-ZNF35(5-8)-IKZF1(2-3)-realDeImmLink-ZIK1KRAB
Plasmid#236142PurposePlasmid encoding the ZNF35/IKZF1 zinc finger array attached by a deimmunized linker to the ZIK1 KRAB domain, under control of CMV promoter with two TetR binding sitesDepositorInsertZNF35(5-8)-IKZF1(2-3)-deImmunLink-ZIK1KRAB
ExpressionMammalianPromoterCMV promoter with two TetR binding sitesAvailable SinceApril 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
pBS-KS-attB1-2-PT-SA-SD-1-mTagBFP2(11x7)
Plasmid#172065PurposeProtein trap plasmid for inserting mTagBFP2(11x7) into a phase 1 coding intronDepositorInsertmTagBFP2(11x7)
ExpressionInsectAvailable SinceOct. 20, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pSEM415 - mosTI unc-119 Pmex-5 - gfp(nls) - tbb-2
Plasmid#182358PurposePositive control for mosTI(unc-119). unc-119 rescue and germline nuclear GFP expression after mosTI insertion.DepositorInsertGFP
ExpressionWormPromoterPmex-5Available SinceMay 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCFJ2211 - [1-2] ENTR - Fluor - mMaple3(intron, PATC, no_atg, no_stop)
Plasmid#159866PurposeThree-fragment Gateway compatible flourophore for expression in C. elegansDepositorInsert[1-2] ENTR - Fluor - mMaple3(intron, PATC, no_atg, no_stop)
ExpressionWormMutationNot applicableAvailable SinceDec. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBS-KS-attB1-2-PT-SA-SD-0-mTagBFP2(11x7)
Plasmid#172064PurposeProtein trap plasmid for inserting mTagBFP2(11x7) into a phase 0 coding intronDepositorInsertmTagBFP2(11x7)
ExpressionInsectAvailable SinceOct. 20, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pSEM414 - mosTI unc-119 Peft-3 - gfp(nls) - tbb-2
Plasmid#182357PurposePositive control for mosTI(unc-119). unc-119 rescue and ubiquitous nuclear GFP expression after mosTI insertion.DepositorInsertGFP
ExpressionWormPromoterPeft-3Available SinceMay 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTYB3-human PUM1-HD MUT3-2 (C935S-Q939E)-intein-CBD
Plasmid#73302PurposeExpresses mutant human PUM1-HDDepositorInserthuman PUM1 Pumilio homology domain (PUM1 Human)
TagsinteinExpressionBacterialMutationC935S-Q939EAvailable SinceMarch 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBXMCS-2 Pconstitutive-sgRNA(Sth3)_cpaA- Pconstitutive-sgRNA(Sth3)_blaA
Plasmid#133347Purposeexpression of two sgRNA from Streptococcus thermophilus #3 each express from its own constitutive promoter; here first one targets cpaA and second one targets blaA (from Caulobacter crescentus)DepositorInsertsgRNA_cpaA and sgRNA_blaA
UseCRISPRPromoterconstitutiveAvailable SinceJan. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBXMCS-2 Pconstitutive-sgRNA(Sth3)_blaA- Pconstitutive-sgRNA(Sth3)_cpaA
Plasmid#133348Purposeexpression of two sgRNA from Streptococcus thermophilus #3 each express from its own constitutive promoter; here first one targets blaA and second one targets cpaA (from Caulobacter crescentus)DepositorInsertsgRNA_blaA and sgRNA_cpaA
UseCRISPRPromoterconstitutiveAvailable SinceJan. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHW564 (Pmyo-2::cGAL-N(C1A)::let-858 3'UTR)
Plasmid#107739Purposemutated cGAL-N (C1A) driver under the control of C. elegans myo-2 promoterDepositorInsertNLS::cGAL(DBD)::gp41-1-N-intein(C1A)
ExpressionWormMutationchange the first cysteine of gp41-1 N-intein to a…PromoterC. elegans myo-2 promoterAvailable SinceMarch 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTYB3-human PUM1-HD MUT7-2 (S1079N-E1083Q)-intein-CBD
Plasmid#73299PurposeExpresses mutant human PUM1-HDDepositorInserthuman PUM1 Pumilio homology domain (PUM1 Human)
TagsinteinExpressionBacterialMutationS1079N-E1083QAvailable SinceFeb. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDisplay-EGFP-2A-HA-Nrxn3-alpha- 2(-)/3(+)/4(+)/6(+)
Plasmid#184334PurposeExpression of mouse Nrx3-alpha lacking insertion at the alternative splice site 4 together with EGFP protein separated by 2A peptide. Contains insertions at alternatively splice sites 1,2,3 and 6.DepositorAvailable SinceJune 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
CA-0248 EF1a_mRens-cageIL-2-c-myc_T2A_ERret-H2M-Renin_T2A-mCherry_Gen2
Plasmid#231208PurposeSingle transcript cageIL-2 (mRens) + ER-renin (H2M) (Lenti)DepositorInsertmRens-cageIL-2-c-myc_T2A_ERret-H2M-Renin_T2A-mCherry (IL2 Human)
UseLentiviralMutationL80F, R81D, L85V, I86V, I92FAvailable SinceJan. 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
Clone 16 - 2 HH06 pFUSE-IgG1-Knob-mutation knob-clone6
Plasmid#192184PurposeClone 16 (HH06) pFUSE-IgG1-Knob-mutation knob-clone6DepositorInsertClone 6 heavy chain (HH06)
ExpressionMammalianMutationNAAvailable SinceDec. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
FUW-crRNA-2-U6_TRE-dLbCpf1-NLS-hCTCF_Q418A-HA-P2A-tagBFP
Plasmid#194895PurposeDox-inducible all-in-one lentiviral construct to express dCpf1-CTCF-Q418A mutant and crRNA-2 targeting the human MECP2 locus.DepositorInsertsdLbCpf1-NLS-hCTCF_Q418A
U6-crRNA2
UseLentiviralTagsHAExpressionMammalianMutationHuman codon optimized catalytically inactive LbCp…PromoterTRE and U6Available SinceApril 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
FUW-crRNA-2-U6_TRE-dLbCpf1-NLS-hCTCF_R396A-HA-P2A-tagBFP
Plasmid#194896PurposeDox-inducible all-in-one lentiviral construct to express dCpf1-CTCF-R396A mutant and crRNA-2 targeting the human MECP2 locus.DepositorInsertsdLbCpf1-NLS-hCTCF_R396A
U6-crRNA2
UseLentiviralTagsHAExpressionMammalianMutationHuman codon optimized catalytically inactive LbCp…PromoterTRE and U6Available SinceJan. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
FUW-crRNA-2-U6_TRE-dLbCpf1-NLS-hCTCF_WT-HA-P2A-tagBFP
Plasmid#194893PurposeDox-inducible all-in-one lentiviral construct to express dCpf1-CTCF-WT and crRNA-2 targeting the human MECP2 locus.DepositorInsertsdLbCpf1-NLS-hCTCF_WT
U6-crRNA2
UseLentiviralTagsHAExpressionMammalianMutationHuman codon optimized catalytically inactive LbCp…PromoterTRE and U6Available SinceJan. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
FUW-crRNA-2-U6_TRE-dLbCpf1-NLS-hCTCF_K365A_R368A-HA-P2A-tagBFP
Plasmid#194894PurposeDox-inducible all-in-one lentiviral construct to express dCpf1-CTCF-K365A_R368A mutant and crRNA-2 targeting the human MECP2 locus.DepositorInsertsdLbCpf1-NLS-hCTCF_K365A_R368A
U6-crRNA2
UseLentiviralTagsHAExpressionMammalianMutationHuman codon optimized catalytically inactive LbCp…PromoterTRE and U6Available SinceJan. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEX-6p-2-GST-PD-1icd (AA194-288, Y223F, Y248F)
Plasmid#180815PurposeExpression of GST fused human PD-1 intracellular domain (AA194-288, Y223F, Y248F)DepositorInsertPD-1int (AA194-288, Y223F, Y248F) (PDCD1 Human)
ExpressionBacterialMutationPD-1 (Y223F, Y248F)PromoterTacAvailable SinceMarch 14, 2022AvailabilityAcademic Institutions and Nonprofits only