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PGK mScarlet-Synaptophysin

Plasmid

#206145

Purpose

PGK mScarlet-Synaptophysin

Depositor

Erika Holzbaur

Article

Dou et al J Cell Biol. 2024 Jun 3;223(6):e20230709

Insert

synaptophysin (Syp Rat)

Tags

mScarlet

Expression

Mammalian

Promoter

PGK

Available Since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3-mCherry-Rheb

Plasmid

#192436

Purpose

Encodes mCherry-tagged wild-type Rheb.

Depositor

Jin Zhang

Article

Zhong et al Cell Chem Biol. 2022 Jun 16;29(6):1037-1

Insert

mCherry-Rheb (RHEB Human)

Tags

6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pFLxR3

Plasmid

#167518

Purpose

Broad-Host range expression vector, RSF1010 origin, LuxR activator, PLuxB promoter, mRFP, Tetracycline Resistance Marker.

Depositor

Christopher Reisch

Article

Schuster et al Nucleic Acids Res. 2021 Jun 14. pii: 629

Insert

LuxR-mRFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

PLuxB

Available Since

Oct. 19, 2021

Availability

Academic Institutions and Nonprofits only

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mCherry-PIK3R1

Plasmid

#174192

Purpose

Expresses PIK3R1 in mammalian cells

Depositor

Cheng-han Yu

Article

Zhou et al J Cell Sci. 2020 Jun 8;133(11). pii: jcs

Insert

PIK3R1 (PIK3R1 Human)

Tags

mCherry

Expression

Mammalian

Available Since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pFAR5

Plasmid

#149478

Purpose

Broad-Host range expression vector, RSF1010 origin, AraC activator/repressor, PBAD promoter, mRFP, Gentamicin Resistance Marker.

Depositor

Christopher Reisch

Article

Schuster et al Nucleic Acids Res. 2021 Jun 14. pii: 629

Insert

AraC-mRFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

PBAD

Available Since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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pWLxR5

Plasmid

#149490

Purpose

Broad-Host range expression vector, incW (pSa) origin, LuxR activator, PLuxB promoter, mRFP, Gentamicin Resistance Marker.

Depositor

Christopher Reisch

Article

Schuster et al Nucleic Acids Res. 2021 Jun 14. pii: 629

Insert

LuxR-mRFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

PLuxB

Available Since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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pEGFP-Alus-Apobec3G

Plasmid

#92348

Purpose

Plasmid for expression of GFP gene with a Apobec3G gene intron derived Alu element containing 3'UTR.

Depositor

Gordon Carmichael, Ling-Ling Chen

Article

Chen et al EMBO J. 2008 Jun 18;27(12):1694-705. doi

Insert

Apobec3G gene intron derived Alu element containing 3'UTR (APOBEC3G Human)

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pBadcrtEBI_13

Plasmid

#63811

Purpose

expresses crtEBI genes for neurosporene production

Depositor

Howard Salis

Article

Farasat et al Mol Syst Biol. 2014 Jun 21;10:731. doi:

Insert

crtEBI operon

Expression

Bacterial

Mutation

Codon optimized for E.coli

Promoter

PBAD

Available Since

May 15, 2015

Availability

Academic Institutions and Nonprofits only

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pFA6a-HTB-TRP1

Plasmid

#26877

Depositor

Peter Kaiser

Article

Tagwerker et al Yeast. 2006 Jun . 23(8):623-32.

Insert

HTB tag

Use

Pcr tagging yeast

Available Since

Dec. 15, 2010

Availability

Academic Institutions and Nonprofits only

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pCMV-PEmax-SpG

Plasmid

#211549

Purpose

Prime editing in mammalian cells

Depositor

Yongsub Kim

Article

Jang et al Commun Biol. 2023 Jun 30;6(1):681. doi:

Insert

PEmax-SpG

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV gfaABC1D NES-jRCaMP1a

Plasmid

#171120

Purpose

Astrocyte AAV-mediated expression of jRCaMP1a, a red fluorescent calcium sensor protein.

Depositor

Thomas Oertner

Article

Lohr et al Front Cell Neurosci. 2021 Jun 11;15:6901

Insert

jRCaMP1a

Use

AAV

Tags

His Tag, T7 Tag, XpressTag

Expression

Mammalian

Promoter

gfaABC1D

Available Since

July 26, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-EPG

Plasmid

#117978

Purpose

Express EPG in mamalian cells

Depositor

Assaf Gilad

Article

Krishnan et al Sci Rep. 2018 Jun 8;8(1):8764. doi: 10.1

Insert

Electromagnetic perceptive gene

Tags

HIS and v5

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pTriEx-mCherry::LANS1

Plasmid

#60784

Purpose

Control of Protein Activity and Cell Fate Specification via Light-Mediated Nuclear Translocation

Depositor

Brian Kuhlman

Article

Yumerefendi et al PLoS One. 2015 Jun 17;10(6):e0128443. do

Insert

LANS1

Tags

mCherry

Expression

Bacterial, Insect, and Mamm…

Promoter

CMV

Available Since

Aug. 6, 2015

Availability

Academic Institutions and Nonprofits only

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Myc-E-Syt3

Plasmid

#66835

Purpose

Mammalian Expression, Bacterial Expression

Depositor

Pietro De Camilli

Article

Giordano et al Cell. 2013 Jun 20;153(7):1494-509. doi:

Insert

E-Syt3 (ESYT3 Human)

Tags

Myc

Expression

Mammalian

Available Since

July 21, 2015

Availability

Academic Institutions and Nonprofits only
pBabe_puro_DEST_Flag_SOX2

Plasmid

#45270

Depositor

William Hahn

Article

Hagerstrand et al Cancer Discov. 2013 Jun 13. [Epub ahead

Insert

MCOPS3 (SOX2 Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Available Since

May 22, 2013

Availability

Academic Institutions and Nonprofits only
APEX2-V5-G3BP1_pTRE-G418

Plasmid

#202013

Purpose

expresses APEX2-G3BP1 in the mammalian cytosol under dox inducible promoter, lentiviral vector

Depositor

Alice Ting

Article

Qin et al Cell. 2023 Jun 23:S0092-8674(23)00596-2.

Insert

APEX2-V5-G3BP1

Use

Lentiviral

Expression

Mammalian

Promoter

pTRE-Tight

Available Since

June 29, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1D/mEGFP + CrPV IGR IRES-nLuc-3XFLAG-CL1PEST

Plasmid

#127332

Purpose

Express 3xFLAG tagged highly destabilized nanoLuciferase (nLuc) protein from CrPV IRES

Depositor

Jeremy Wilusz

Article

Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

Insert

nanoLuciferase

Tags

3xFLAG and CL1/PEST

Expression

Mammalian

Promoter

CMV

Available Since

June 26, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA-TdT

Plasmid

#126450

Purpose

Expresses human TdT in mammalian cells. (pTBL206)

Depositor

Chang Liu

Article

Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

Insert

TdT (DNTT Human)

Expression

Mammalian

Promoter

CMV

Available Since

June 4, 2019

Availability

Academic Institutions and Nonprofits only

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pMaCTag-07

Plasmid

#124786

Purpose

Template for C-terminal PCR tagging of mammalian genes (without selection) with mNeonGreen

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

mNeonGreen

Use

Pcr template

Tags

mNeonGreen

Promoter

None

Available Since

May 7, 2019

Availability

Academic Institutions and Nonprofits only

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pLenti HRE mutant-Luc pGK Hygro

Plasmid

#118707

Purpose

reporter plasmid, HIF-responsive promoter (3xHRE) mutant- fused to firefly luciferase

Depositor

William Kaelin

Article

Briggs et al Cell. 2016 Jun 30;166(1):126-39. doi: 10

Insert

HREmutant-Luc

Use

Lentiviral

Available Since

Nov. 26, 2018

Availability

Academic Institutions and Nonprofits only

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