We narrowed to 3,108 results for: N-EGFP

1. pACYC-T7-SpCas9(BspMI/SapI/BsaI_cassettes)-T7-gRNA1 (BPK1807)

   Plasmid

   #223065

   Purpose

   Dual pT7 entry plasmid for SpCas9(6AA) library; bacterial expression plasmid with type IIS RE cassettes around D1135/S1136, G1218/E1219, R1335/T1337 (precursor to MMW94). Expresses a gRNA.

   Depositor

   Benjamin Kleinstiver

   Article

   Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

   Insert

   entry vector for human codon opt. bacterial expr. plasmid for SpCas9(6AA_NNS) library, with gRNA targeting EGFP site 1
   

   Use

   CRISPR

   Tags

   NLS(SV40)-3xFLAG

   Expression

   Bacterial

   Mutation

   Three regions of SpCas9 encode type IIS restricti…

   Promoter

   Dual T7

   Available Since

   April 23, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. MADR pDonor smFP-Myc (dark) WPRE

   Plasmid

   #130987

   Purpose

   MADR donor plasmid for FlpO+Cre- mediated insertion of a SM_FP-Myc, a cytoplasmic fluorescent protein which includes multiple Myc epitope tags

   Depositor

   Joshua Breunig

   Article

   Kim et al Cell. 2019 Sep 19;179(1):251-267.e24. do

   Insert

   smFP-Myc WPRE
   

   Use

   Mosaic analysis for dual recombinase-mediated cas…

   Tags

   Myc - 10 total Myc tags

   Expression

   Mutation

   "dark" variant with GGG fluorophore ver…

   Promoter

   none (4x polyA to mitigate episomal expression)

   Available Since

   July 8, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. retro-gfpIkkb-puro vector

   Plasmid

   #58251

   Purpose

   Retroviral expression vector encoding bicistronic EGFP-IKKbeta and Puromycin cassette

   Depositor

   Georgios Stathopoulos

   Article

   Giannou et al J Clin Invest. 2015 Jun;125(6):2317-34.

   Insert

   Ikkβ (Ikbkb Mouse)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   March 30, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. nls-NgAgo-GK

   Plasmid

   #78253

   Purpose

   Mammalian expression of NgAgo (Argonaute from N. gregoryi).

   Depositor

   Chunyu Han

   Article

   Gao et al Nat Biotechnol. 2016 May 2. doi: 10.1038

   Insert

   NgAgo
   

   Use

   Tags

   a nuclear localization signal (NLS)

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   June 2, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAN008

   Plasmid

   #220049

   Purpose

   Reporter plasmid that encodes for the GFP fused to an N-terminal flag-HA epitope (positive control).

   Depositor

   Blake Wiedenheft

   Article

   Nemudraia et al Science. 2024 Apr 25:eadk5518. doi: 10.1

   Insert

   flag-HA-GFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   May 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. TV-hPITX3-Reverse

   Plasmid

   #22208

   Depositor

   Rudolf Jaenisch

   Article

   Hockemeyer et al Nat Biotechnol. 2009 Sep;27(9):851-7. do

   Insert

   PITX3-eGFP-loxP-PGK-Puro-loxP (PITX3, PGK1 Human)
   

   Use

   Zfn donor

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Oct. 5, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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7. pNLS-LEXY (pDN138)

   Plasmid

   #72657

   Purpose

   golden gate entry vector for mCherry-LEXY tagging of proteins (N-terminal cMyc(P1A) NLS)

   Depositor

   Barbara Di Ventura, Roland Eils

   Article

   Niopek et al Nat Commun. 2016 Feb 8;7:10624. doi: 10.

   Type

   Empty backbone

   Use

   Golden gate entry vector

   Tags

   NLS and mCherry-LEXY

   Expression

   Mammalian

   Mutation

   Promoter

   CMV-F

   Available Since

   Feb. 9, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pmGFP-Sec16L

   Plasmid

   #15776

   Depositor

   Benjamin Glick

   Article

   Bhattacharyya et al Mol Biol Cell. 2007 Mar;18(3):839-49. do

   Insert

   Sec16L (SEC16A Human)
   

   Use

   Tags

   mGFP

   Expression

   Mammalian

   Mutation

   N/A

   Promoter

   Available Since

   Nov. 21, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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9. SuperNova-N1

   Plasmid

   #131853

   Purpose

   Expresses SuperNova with C-terminal Myc-tag and N-terminal multiple cloning site

   Depositor

   Geert van den Bogaart

   Article

   Paardekooper et al Int J Mol Sci. 2019 Aug 25;20(17). pii:

   Insert

   SuperNova
   

   Use

   Tags

   Myc tag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Oct. 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAN007

    Plasmid

    #220048

    Purpose

    Reporter plasmid that encodes for the GFP fused to an N-terminal flag-HA epitope. A stop codon is inserted between the epitope tag and the gfp sequence to terminate GFP translation.

    Depositor

    Blake Wiedenheft

    Article

    Nemudraia et al Science. 2024 Apr 25:eadk5518. doi: 10.1

    Insert

    flag-HA-stop-GFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. 2COX8-BmTyr

    Plasmid

    #222943

    Purpose

    mitochondrion-localized BmTyr

    Depositor

    Itaru Hamachi

    Article

    Zhu et al J Am Chem Soc. 2024 Mar 20;146(11):7515-

    Insert

    2COX8-BmTyr-Flag
    

    Use

    Tags

    Flag tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. IgK-BmTyr-KDEL

    Plasmid

    #222942

    Purpose

    ER-localized BmTyr

    Depositor

    Itaru Hamachi

    Article

    Zhu et al J Am Chem Soc. 2024 Mar 20;146(11):7515-

    Insert

    IgK-BmTyr-Flag-KDEL
    

    Use

    Tags

    Flag tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. BmTyr-Flag

    Plasmid

    #220779

    Purpose

    non-targeted BmTyr throughout the cytoplasm

    Depositor

    Itaru Hamachi

    Article

    Zhu et al J Am Chem Soc. 2024 Mar 20;146(11):7515-

    Insert

    Bacillus megaterium Tyrosinase
    

    Use

    Tags

    Flag tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Sept. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. mCherry-TrioN

    Plasmid

    #129621

    Purpose

    RhoGEF

    Depositor

    Dorus Gadella

    Article

    Reinhard et al Small GTPases. 2019 Aug 30:1-15. doi: 10

    Insert

    N-terminal part of TRIO
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Jan. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. dimerTomato-wGBD

    Plasmid

    #129635

    Purpose

    Visualization of active Cdc42

    Depositor

    Dorus Gadella

    Article

    Mahlandt et al J Cell Sci. 2023 May 15;136(10):jcs26080

    Insert

    Cdc42 binding domain of N-WASP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMVdel

    Available Since

    April 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. FKBP-AP180-GFP

    Plasmid

    #186577

    Purpose

    Expression of the fusion protein FKBP-AP180-GFP. Uses rat AP180 residues 328-896.

    Depositor

    Stephen Royle

    Article

    Kuey et al Elife. 2022 Jul 19;11. pii: 78929. doi:

    Insert

    AP180 (Snap91 Rat)
    

    Use

    Tags

    FKBP N-terminal GFP C-terminal

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. Flag-nls-NgAgo-GK

    Plasmid

    #86435

    Purpose

    Expresses a Flag tag on the N-terminal

    Depositor

    Gaétan Burgio

    Article

    Khin et al PLoS One. 2017 Jun 13;12(6):e0178768. do

    Insert

    NgAgo
    

    Use

    Tags

    Flag tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    May 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pmStayGold_I3

    Plasmid

    #239336

    Purpose

    Expresses the I3-01 nanocage subunit N-terminally tagged with mStayGold in mammalian cells. Assembles into nanocages tagged with 60 FPs.

    Depositor

    Torsten Wittmann

    Article

    Viola et al J Cell Sci. 2025 May 15;138(10):jcs26385

    Insert

    I3-01
    

    Use

    Tags

    mStayGold

    Expression

    Mammalian

    Mutation

    K129A

    Promoter

    CMV

    Available Since

    June 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pmScarlet3_I3

    Plasmid

    #239342

    Purpose

    Expresses the I3-01 nanocage subunit N-terminally tagged with mScarlet3 in mammalian cells. Assembles into nanocages tagged with 60 FPs.

    Depositor

    Torsten Wittmann

    Article

    Viola et al J Cell Sci. 2025 May 15;138(10):jcs26385

    Insert

    I3-01
    

    Use

    Tags

    mScarlet3

    Expression

    Mammalian

    Mutation

    K129A

    Promoter

    CMV

    Available Since

    June 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. CytERM-dTomato

    Plasmid

    #98834

    Purpose

    In vivo visualization of the ER (can be used for colocalization studies and the OSER assay)

    Depositor

    Dorus Gadella

    Article

    Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

    Insert

    CytERM
    

    Use

    Tags

    dTomato

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Aug. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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