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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 5,111 results for: codon optimized

Showing: 2861 - 2880 of 5111 results

  1. pCAG-nucGFP11-3xPax7gRNA

    Plasmid
    #224570
    Purpose
    Ubiquitous expression plasmid, CAG promoter (CMV immediate early enhancer, chicken beta actin promoter), three unique Pax7 gRNAs with ribozyme self-cleavage sites, and nuclear split GFP(11) reporter.
    Depositor
    Erica Hutchins
    Article
    Kim et al Dev Biol. 2024 Nov 30:S0012-1606(24)0026
    Insert
    GFP11-H2B
    Use
    CRISPR
    Tags
    Histone H2B (nuclear localization)
    Mutation
    Codon optimized
    Available Since
    Dec. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    224570-plasmid-map-sequence-id-451492

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  2. pCAG-3xGFP11-mem-3xPax7gRNA

    Plasmid
    #224571
    Purpose
    Ubiquitous expression plasmid with CAG promoter (CMV immediate early enhancer, chicken beta actin promoter), three unique Pax7 gRNAs with ribozyme self-cleavage, three membrane split GFP(11) reporter.
    Depositor
    Erica Hutchins
    Article
    Kim et al Dev Biol. 2024 Nov 30:S0012-1606(24)0026
    Insert
    3xGFP11
    Use
    CRISPR
    Tags
    Membrane Localization Signal
    Mutation
    Codon optimized
    Available Since
    Dec. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    224571-plasmid-map-sequence-id-451493

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  3. Sc++

    Plasmid
    #198545
    Purpose
    Expresses human codon-optimized Sc++ and blasticidin resistance: EFS promoter-Sc++-NLS-FLAG-P2A-BSD
    Depositor
    Hyongbum Kim
    Article
    Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.
    Insert
    Sc++
    Use
    CRISPR and Lentiviral
    Tags
    BSD, FLAG, and NLS
    Expression
    Mammalian
    Promoter
    EFS
    Available Since
    Nov. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    198545-plasmid-map-sequence-id-388902

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  4. SpG

    Plasmid
    #198543
    Purpose
    Expresses human codon-optimized SpG and blasticidin resistance: EFS promoter-SpG-NLS-FLAG-P2A-BSD
    Depositor
    Hyongbum Kim
    Article
    Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.
    Insert
    SpG
    Use
    CRISPR and Lentiviral
    Tags
    BSD, FLAG, and NLS
    Expression
    Mammalian
    Promoter
    EFS
    Available Since
    Nov. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    198543-plasmid-map-sequence-id-388887

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  15. GB3668

    Plasmid
    #215243
    Purpose
    TU for the expression of mAID (N-tag): AcrIIA4 w/o NLS protein Codon optimized for N. benthamiana expression under the regulation of the 35S promoter and TNOS terminator
    Depositor
    Diego Orzaez
    Article
    Calvache et al Plant Biotechnol J. 2024 Jan;22(1):37-47
    Insert
    P35S:mAID:AcrIIA4:TNOS
    Use
    Synthetic Biology
    Mutation
    BsaI and BsmBI sites removed
    Available Since
    June 18, 2024
    Availability
    Academic Institutions and Nonprofits only
    215243-plasmid-map-sequence-id-436560

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  16. GB1531

    Plasmid
    #215242
    Purpose
    TU for constitutive expression of Streptomyces phage phiC31 integrase. Catalyzes site-specific recombination between phiC31 attB and attP sites. Contains SV40 NLS. N. benthamiana codon optimized.
    Depositor
    Diego Orzaez
    Article
    Calvache et al Plant Biotechnol J. 2024 Jan;22(1):37-47
    Insert
    PNos:PhiC31:TNos
    Use
    Synthetic Biology
    Mutation
    BsaI and BsmBI sites removed
    Available Since
    June 18, 2024
    Availability
    Academic Institutions and Nonprofits only
    215242-plasmid-map-sequence-id-436558

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  20. mFzd4CRDL(42-203)-6xHis_pAcGP67a

    Plasmid
    #216379
    Purpose
    Baculovirus transfer vector to secrete Fzd4 cysteine-rich domain and linker (residues 42-203, codon-optimized for insect cell expression, with a C-terminal His tag) in insect cells
    Depositor
    William Weis
    Article
    Bruguera et al bioRxiv 2024.02.03.578714
    Insert
    Frizzled-4 (Fzd4 Mouse)
    Use
    Baculovirus
    Tags
    6xHis and GP64 signal sequence
    Expression
    Insect
    Promoter
    Polyhedrin
    Available Since
    May 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    216379-plasmid-map-sequence-id-433261

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Showing: 2861 - 2880 of 5111 results