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pET19b_4CL:P7S + STS:P8S

Plasmid

#139794

Purpose

expression of biosynthetic enzymes 4CL and STS in fusion with CC

Depositor

Roman Jerala

Article

Fink et al AMB Express. 2020 May 24;10(1):97. doi:

Inserts

4CL:P7S
STS:P8S

Expression

Bacterial

Available Since

June 1, 2020

Availability

Academic Institutions and Nonprofits only

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pNOC-stacked-GeNL-Ca

Plasmid

#101003

Purpose

Construct for fusion expression of GeNL calcium reporter and neomycin/G418 resistance gene by a bidirectional promoter

Depositor

Eva Farre

Article

Poliner et al Plant J. 2019 Jul;99(1):112-127. doi: 10

Type

Empty backbone

Use

Algae, nannochloropsis expression

Available Since

May 31, 2019

Availability

Academic Institutions and Nonprofits only

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pJK504

Plasmid

#71584

Purpose

Produces Acetobacter aceti 1023 succinyl-CoA:acetate CoA-transferase (AarC) with C-terminal His6 tag and Cys357>Tyr mutant (AarCH6-C357Y)

Depositor

T. J. Kappock

Article

Mullins et al Acetic Acid Bacteria. 2(s1):e3 (2013)

Insert

succinyl-CoA:acetate CoA-transferase

Tags

His6

Expression

Bacterial

Mutation

changes cysteine-357 to tyrosine

Promoter

T7

Available Since

Feb. 26, 2016

Availability

Academic Institutions and Nonprofits only

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pJK274

Plasmid

#71564

Purpose

Produces Crepis alpina delta12 fatty acid desaturase (FAD2-3) in yeast

Depositor

T. J. Kappock

Article

Nam et al J Exp Bot. 2007;58(6):1421-32. Epub 2007

Insert

delta12 fatty acid desaturase (form 3)

Expression

Bacterial and Yeast

Promoter

GAL1 promoter

Available Since

Jan. 5, 2016

Availability

Academic Institutions and Nonprofits only

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pGGF004

Plasmid

#48845

Purpose

Provides a D-alanine resistance cassette as GreenGate module.

Depositor

Jan Lohmann

Article

Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

Insert

p35S::D-AlanineR::t35S

Use

Golden gate compatible cloning vector

Mutation

internal BsaI recognition site in 35S promoter re…

Available Since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pGGA002

Plasmid

#48813

Purpose

Provides the AP3 promoter as GreenGate module.

Depositor

Jan Lohmann

Article

Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

Insert

AP3 promoter

Use

Golden gate compatible cloning vector

Mutation

internal BsaI recognition site removed by substit…

Available Since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

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UAS-zw4 (egghead) ORF

Plasmid

#38764

Depositor

Norbert Perrimon

Article

Goode et al Development. 1996 Dec;122(12):3863-79.

Insert

zw4 (egh Fly)

Expression

Insect

Promoter

Hsp70

Available Since

Aug. 29, 2012

Availability

Academic Institutions and Nonprofits only
pCaShs-egghead (zw4)

Plasmid

#37400

Depositor

Norbert Perrimon

Article

Goode et al Development. 1996 Dec;122(12):3863-79.

Insert

egghead (egh Fly)

Expression

Insect

Promoter

Hsp70

Available Since

July 16, 2012

Availability

Academic Institutions and Nonprofits only
pSKB2-TRexR90D

Plasmid

#31350

Depositor

Clara Kielkopf

Article

McLaughlin et al Mol Cell. 2010 May 28. 38(4):563-75.

Insert

Thermus aquaticus Redox Sensing Repressor (R90D)

Tags

His

Expression

Bacterial

Mutation

changed Arginine 90 to Aspartic Acid

Available Since

Aug. 18, 2011

Availability

Academic Institutions and Nonprofits only
pTA7001-DEST

Plasmid

#71745

Purpose

GATEWAY compatible derivative of plant binary vector pTA7001 from Nam Chua's laboratory at The Rockefeller University, Dexamethasone inducible

Depositor

Roger Innes

Article

Gu et al Plant Physiol. 2011 Apr;155(4):1827-38.

Type

Empty backbone

Expression

Plant

Promoter

GAL4

Available Since

Feb. 11, 2016

Availability

Academic Institutions and Nonprofits only

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