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pmTagBFP2(GGS)-Fb(LAM2)

Plasmid

#249305

Purpose

pmTagBFP2(GGS)-Fb(LAM2) expression in mammalian cells.

Depositor

Vladislav Verkhusha

Article

Barykina et al Nat Methods. 2026 Apr 22. doi: 10.1038/s

Insert

pmTagBFP2(GGS)-Fb(LAM2)

Expression

Mammalian

Promoter

CMV

Available Since

April 22, 2026

Availability

Academic Institutions and Nonprofits only

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pEF-1a_RfxCas13d-HA-IRES-BFP-2A-BSD

Plasmid

#251220

Purpose

Lentiviral transfer plasmid for the generation of EF1a driven RfxCas13d expressing cells

Depositor

Niels Geijsen

Article

Bot et al Commun Biol. 2026 Jan 19;9(1):233. doi:

Insert

RfxCas13d

Use

CRISPR and Lentiviral

Tags

HA

Expression

Mammalian

Promoter

EF-1a

Available Since

March 30, 2026

Availability

Academic Institutions and Nonprofits only

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pEF1-a_LbuCas13a-HA-IRES-BFP-2A-BSD

Plasmid

#251219

Purpose

Lentiviral transfer plasmid for the generation of EF1a driven LbuCas13a expressing cells.

Depositor

Niels Geijsen

Article

Bot et al Commun Biol. 2026 Jan 19;9(1):233. doi:

Insert

LbuCas13a

Use

CRISPR and Lentiviral

Tags

HA

Expression

Mammalian

Promoter

EF-1a

Available Since

March 30, 2026

Availability

Academic Institutions and Nonprofits only

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pEF-1a_dEGFP-Y66S-IRES-PuroR-WPRE

Plasmid

#251210

Purpose

Lentiviral transfer plasmid for the generation of EF1a driven dEGFP-Y66S expressing cells. Puromycin selection marker.

Depositor

Niels Geijsen

Article

Bot et al Commun Biol. 2026 Jan 19;9(1):233. doi:

Insert

dEGFP-Y66S

Use

Lentiviral

Expression

Mammalian

Mutation

Y66S

Promoter

EF-1a

Available Since

March 30, 2026

Availability

Academic Institutions and Nonprofits only

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pSSBIO32_dCas9_RPA0650

Plasmid

#240487

Purpose

CRISPRi-mediated Knockdown of RPA0650

Depositor

Rajib Saha

Article

Kathol et al Appl Environ Microbiol. 2025 Aug 20;91(8

Insert

Cas9 (cas9 Streptococcus pyogenes)

Use

CRISPR

Expression

Bacterial

Mutation

D10A, H840A

Promoter

Plac

Available Since

Dec. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pSSBIO32_dCas9_RPA3717

Plasmid

#240491

Purpose

CRISPRi-mediated Knockdown of RPA3717

Depositor

Rajib Saha

Article

Kathol et al Appl Environ Microbiol. 2025 Aug 20;91(8

Insert

Cas9 (cas9 Streptococcus pyogenes)

Use

CRISPR

Expression

Bacterial

Mutation

D10A, H840A

Promoter

Plac

Available Since

Dec. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pSSBIO32_dCas9_RPA3701

Plasmid

#240490

Purpose

CRISPRi-mediated Knockdown of RPA3701

Depositor

Rajib Saha

Article

Kathol et al Appl Environ Microbiol. 2025 Aug 20;91(8

Insert

Cas9 (cas9 Streptococcus pyogenes)

Use

CRISPR

Expression

Bacterial

Mutation

D10A, H840A

Promoter

Plac

Available Since

Dec. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pSSBIO32_dCas9_RPA1580

Plasmid

#240488

Purpose

CRISPRi-mediated Knockdown of RPA1580

Depositor

Rajib Saha

Article

Kathol et al Appl Environ Microbiol. 2025 Aug 20;91(8

Insert

Cas9 (cas9 Streptococcus pyogenes)

Use

CRISPR

Expression

Bacterial

Mutation

D10A, H840A

Promoter

Plac

Available Since

Dec. 2, 2025

Availability

Academic Institutions and Nonprofits only

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RcP9

Plasmid

#242157

Purpose

Recombineering Plasmid. ITPG-induced expression of Recombitron operon (ccdB dropout cassette in Eco1 Retron); PSP-Tet promoter for Gene III induction upon M13 phage infection and aTc addition.

Depositor

Dominik Niopek

Article

Southern et al Nat Commun. 2026 Apr 14;17(1):3498. doi:

Type

Empty backbone

Expression

Bacterial

Available Since

Dec. 2, 2025

Availability

Academic Institutions and Nonprofits only

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PACE_SP_SD4_AraC_R5_S170_LOV

Plasmid

#242146

Purpose

Contains an AraC R5 Variant-LOV Chimera gene to assemble a selection phage missing gene III with SapI Golden Gate Assembly

Depositor

Dominik Niopek

Article

Southern et al Nat Commun. 2026 Apr 14;17(1):3498. doi:

Insert

AraC R5 S170 LOV

Expression

Bacterial

Available Since

Aug. 26, 2025

Availability

Academic Institutions and Nonprofits only

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pDest_PANCEd_AraC_S170_LOV_sd2_R2_C1

Plasmid

#242152

Purpose

Expression Plasmid containing a LacI repressed sd2 AraC R2 PANCE cassette with a LOV domain insertion at S170

Depositor

Dominik Niopek

Article

Southern et al Nat Commun. 2026 Apr 14;17(1):3498. doi:

Insert

AraC R2 S170 LOV

Expression

Bacterial

Mutation

E3K, R146L, A152T, R251L

Available Since

Aug. 26, 2025

Availability

Academic Institutions and Nonprofits only

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pDest_PANCEd_AraC_S170_LOV_sd2_WT

Plasmid

#242151

Purpose

Expression Plasmid containing a LacI repressed sd2 WT AraC cassette with a LOV domain insertion at S170

Depositor

Dominik Niopek

Article

Southern et al Nat Commun. 2026 Apr 14;17(1):3498. doi:

Insert

AraC S170 LOV

Expression

Bacterial

Available Since

Aug. 26, 2025

Availability

Academic Institutions and Nonprofits only

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PACE_SP_SD4_AraC_R2_S170_LOV

Plasmid

#242145

Purpose

Contains an AraC R2 Variant-LOV Chimera gene to assemble a selection phage missing gene III with SapI Golden Gate Assembly

Depositor

Dominik Niopek

Article

Southern et al Nat Commun. 2026 Apr 14;17(1):3498. doi:

Insert

AraC R2 S170 LOV

Expression

Bacterial

Available Since

Aug. 26, 2025

Availability

Academic Institutions and Nonprofits only

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PACE_SP_SD4_AraC_S170_LOV

Plasmid

#242144

Purpose

Contains an AraC-LOV Chimera gene to assemble a selection phage missing gene III with SapI Golden Gate Assembly

Depositor

Dominik Niopek

Article

Southern et al Nat Commun. 2026 Apr 14;17(1):3498. doi:

Insert

AraC S170 LOV

Expression

Bacterial

Available Since

Aug. 26, 2025

Availability

Academic Institutions and Nonprofits only

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pDest_PANCEd_AraC_S170_LOV_sd2_R5_C1

Plasmid

#242153

Purpose

Expression Plasmid containing a LacI repressed sd2 AraC R5 PANCE cassette with a LOV domain insertion at S170

Depositor

Dominik Niopek

Article

Southern et al Nat Commun. 2026 Apr 14;17(1):3498. doi:

Insert

AraC R5 S170 LOV

Expression

Bacterial

Mutation

A2E, L156I, R251H

Available Since

Aug. 26, 2025

Availability

Academic Institutions and Nonprofits only

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pMV261-igr3K253E

Plasmid

#225739

Purpose

To express igr3 (Rv3542c-Rv3541c-Rv3540c) with K253E mutation in Rv3542c in Mycobacteria

Depositor

Nicole Sampson

Article

Panagoda et al ACS Chem Biol. 2024 Dec 20;19(12):2580-2

Insert

Myc-igr3K253E

Tags

c-Myc

Expression

Bacterial

Mutation

igr3 K253E

Available Since

Jan. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pMV261-igr3WT

Plasmid

#225738

Purpose

To express wild type igr3 (Rv3542c-Rv3541c-Rv3540c) in Mycobacteria

Depositor

Nicole Sampson

Article

Panagoda et al ACS Chem Biol. 2024 Dec 20;19(12):2580-2

Insert

Myc-igr3WT

Tags

c-Myc

Expression

Bacterial

Available Since

Oct. 28, 2024

Availability

Academic Institutions and Nonprofits only

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pGAL4-sfGFP(1-157)

Plasmid

#220743

Purpose

GAL4-sfGFP(1-157) construct expression in mammlian cells.

Depositor

Vladislav Verkhusha

Article

Barykina et al Nat Commun. 2024 Sep 6;15(1):7788. doi:

Insert

GAL4-sfGFP(1-157)

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pGP-mTagBFP2

Plasmid

#220747

Purpose

mTagBFP2 expression in mammalian cells. Reporter plasmid.

Depositor

Vladislav Verkhusha

Article

Barykina et al Nat Commun. 2024 Sep 6;15(1):7788. doi:

Insert

mTagBFP2

Expression

Mammalian

Promoter

12xUAS with TATA-box minimal promoter

Available Since

Sept. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pNIR-Fb(718)

Plasmid

#220742

Purpose

NIR-Fb(718) expression in mammalian cells.

Depositor

Vladislav Verkhusha

Article

Barykina et al Nat Commun. 2024 Sep 6;15(1):7788. doi:

Insert

NIR-Fb(718)

Use

Affinity Reagent/ Antibody

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 9, 2024

Availability

Academic Institutions and Nonprofits only

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