We narrowed to 9,626 results for: otos

1. pET28a-SrtAdelta59

   Plasmid

   #51138

   Purpose

   expression plasmid for N-terminal his tagged S.aureus SrtA delta 59

   Depositor

   Hidde Ploegh

   Article

   Guimaraes et al Nat Protoc. 2013 Sep;8(9):1787-99. doi:

   Insert

   S.aureus SrtA delta 59
   

   Use

   Tags

   6x His

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Feb. 24, 2014

   Availability

   Academic Institutions and Nonprofits only

2. AAV-CAG-hChR2_tdTomato

   Plasmid

   #28015

   Purpose

   Expresses humanized ChR2 fused to tdTomato for optogenetic activation

   Depositor

   Karel Svoboda

   Article

   Mao et al Neuron. 2011 Oct 6;72(1):111-23.

   Insert

   humanized ChR2-tdTomato
   

   Use

   AAV; Adeno associate viral

   Tags

   tdTomato

   Expression

   Mutation

   Promoter

   CAG

   Available since

   Oct. 27, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMRX-IP-3xFLAG-NCOA4

   Plasmid

   #192807

   Purpose

   Stable expression of 3xFLAG-NCOA4 in mammalian cells

   Depositor

   Noboru Mizushima

   Article

   Ohshima et al J Cell Biol. 2022 Oct 3;221(10). pii: 21

   Insert

   NCOA4 (NCOA4 Human)
   

   Use

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMA3174

   Plasmid

   #46880

   Purpose

   Lentiviral vector for the dual control of expression through Tet-On promoter and C-terminal destabilizing domain

   Depositor

   Mikhail Alexeyev, Troy Stevens

   Article

   Ochoa et al J Biol Chem. 2012 Jul 20;287(30):25407-1

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   FFKBP12 E31G, R71G, K105E and myc

   Expression

   Mutation

   Promoter

   TRE-Tight

   Available since

   Aug. 20, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. pFN18A-HaloTag-SpyCatcher

   Plasmid

   #206041

   Purpose

   HaloTag-Spy0128-(Protein of Interest)-Spy0128-SpyCatcher-8xHisTag

   Depositor

   Sergi Garcia-Manyes

   Article

   Tapia-Rojo et al Nat Protoc. 2024 Mar 11. doi: 10.1038/s4

   Type

   Empty backbone

   Use

   Tags

   HaloTag and SpyCatcher-8His

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   March 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCDNA HA FAK Y397F

   Plasmid

   #50503

   Depositor

   Kenneth Yamada

   Article

   Tamura et al J Biol Chem. 1999 Jul 16;274(29):20693-7

   Insert

   protein tyrosine kinase, mutated
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Y397F

   Promoter

   CMV

   Available since

   Jan. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

7. pAAV_CMV::V5-EGFR

   Plasmid

   #199717

   Purpose

   Expresses V5-tagged EGFR in mammalian cells.

   Depositor

   Alice Ting

   Article

   Roh et al J Am Chem Soc. 2023 May 10;145(18):10220

   Insert

   V5-EGFR
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMXs-OLIG2

   Plasmid

   #32933

   Depositor

   Kevin Eggan

   Article

   Son et al Cell Stem Cell. 2011 Sep 2;9(3):205-18.

   Insert

   OLIG2 (OLIG2 Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   5'LTR

   Available since

   Nov. 30, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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9. px459V2.0_Conc2

   Plasmid

   #134451

   Purpose

   Empty concatemer vector in which 2 sgRNAs can be inserted; with Cas9 + Puro

   Depositor

   Daniel Stange

   Article

   Gaebler et al J Vis Exp. 2020 Feb 18;(156). doi: 10.37

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Jan. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. mOrange2-Lysosomes-20

    Plasmid

    #57968

    Purpose

    Localization: Lysosome Membrane, Excitation: 549, Emission: 565

    Depositor

    Michael Davidson

    Article

    Shaner et al Nat Methods. 2008 Jun;5(6):545-51. doi:

    Insert

    LAMP1
    

    Use

    Tags

    mOrange2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 24, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. mEGFP-8xbiRhoBAST

    Plasmid

    #196351

    Purpose

    Expression of mEGFP mRNA with 8xbiRhoBAST in 3'UTR

    Depositor

    Murat Sunbul

    Article

    Buhler et al Nat Chem Biol. 2023 Apr;19(4):478-487. d

    Insert

    mEGFP-8xbiRhoBAST
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. CellTagB

    Plasmid

    #124592

    Purpose

    Expressed barcode for sample multiplexing in single-cell analysis

    Depositor

    Samantha Morris

    Article

    Guo et al Genome Biol. 2019 May 9;20(1):90. doi: 1

    Insert

    CellTagB
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hCMV

    Available since

    April 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. Actinin-sstFRET-GR

    Plasmid

    #83416

    Purpose

    FRET-based actinin tension sensor

    Depositor

    Tetsuya Kitaguchi

    Article

    Wang et al J Biomech Sci Eng (2016). Vol.11, No.4

    Insert

    Actinin-sstFRET-GR
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 17, 2016

    Availability

    Academic Institutions and Nonprofits only

14. pCDNA HA FAK Y407F

    Plasmid

    #50504

    Depositor

    Kenneth Yamada

    Article

    Tamura et al J Biol Chem. 1999 Jul 16;274(29):20693-7

    Insert

    protein tyrosine kinase, mutated
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Y407F

    Promoter

    CMV

    Available since

    Jan. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

15. pBR322-Pdp1-HiBiT

    Plasmid

    #198310

    Purpose

    Loads HiBiT-tagged Pdp1 into PVCs; for payload loading assays; also contains Pnf

    Depositor

    Feng Zhang

    Article

    Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

    Insert

    Pdp1-HiBiT
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. negActinin-sstFRET-GR

    Plasmid

    #83417

    Purpose

    FRET-based actinin tension sensor (negative control)

    Depositor

    Tetsuya Kitaguchi

    Article

    Wang et al J Biomech Sci Eng (2016). Vol.11, No.4

    Insert

    negActinin-sstFRET
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 17, 2016

    Availability

    Academic Institutions and Nonprofits only

17. pX330GFP-hU6-gRNA-hSpCas9

    Plasmid

    #128385

    Purpose

    GFP positive gRNA/hSpCas9 construct

    Depositor

    Budd A. Tucker

    Article

    Giacalone et al Curr Protoc Stem Cell Biol. 2018 Feb 28;

    Insert

    GFP, gRNA and hSpCas9
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pBTK1024 (pDS-Def1)

    Plasmid

    #183125

    Purpose

    Expresses double-stranded RNA targeting the Def1 gene from Apis mellifera

    Depositor

    Jeffrey Barrick

    Article

    Lariviere et al Nat Protoc. 2023 Mar;18(3):902-928. doi:

    Insert

    Fragment of Def1 gene
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3-YFP-PKD2

    Plasmid

    #31821

    Depositor

    Alexandra Newton

    Article

    Kunkel et al J Biol Chem. 2009 Sep 4. 284(36):24653-6

    Insert

    YFP-Protein Kinase D2 (PRKD2 Human)
    

    Use

    Tags

    YFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 4, 2011

    Availability

    Academic Institutions and Nonprofits only

20. Human Bcl-2/pcDNA3

    Plasmid

    #19279

    Depositor

    Philip Leder

    Article

    Fantin et al Cancer Res. 2004 Jan 1. 64(1):329-36.

    Insert

    human Bcl-2 (BCL2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 15, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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