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pHRSV_CHARMS-20xTA

Plasmid

#233935

Purpose

Mammalian expression of Chimeric Higher-Order Assemblies for Receptor Mediated Signaling (CHARMS) variant: CHARMS-10xTAcontains 20 TA repeats in place of the death domain

Depositor

Marcus Taylor

Article

Lichtenstein et al bioRxiv 2024.05.08.593169

Insert

CHARMS-20xTA

Use

Lentiviral

Tags

mEGFP

Expression

Mammalian

Promoter

SV40

Available Since

April 28, 2025

Availability

Academic Institutions and Nonprofits only

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pNMTMA_MBP-Z-Cav1-His6

Plasmid

#223701

Purpose

To produce z-spheres (caveospheres containing the anti-IgG Z-domain of protein A), Parton Lab clone LCU

Depositor

Rob Parton

Article

T. D. et al bioRxiv

Insert

MBP-Z-Cav1-His6

Expression

Bacterial

Available Since

Oct. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pNoT7

Plasmid

#218168

Purpose

pET31b plasmid with short insert lacking T7 and ribosome binding sites as control

Depositor

Emily Weinert

Article

Chauhan et al ACS Cent Sci. 2022 Nov 23;8(11):1518-152

Insert

Short sequence, mutated RBS and T7 sites

Expression

Bacterial

Promoter

NA - mutated T7

Available Since

June 3, 2024

Availability

Academic Institutions and Nonprofits only

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pMJA008

Plasmid

#128512

Purpose

Expresses Xylanase C

Depositor

Marcos Alcocer

Article

Furniss et al Biochim Biophys Acta. 2002 Jul 29;1598(1

Insert

Xylanase C

Expression

Bacterial

Promoter

Histone 4B

Available Since

July 26, 2019

Availability

Academic Institutions and Nonprofits only

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pAW228

Plasmid

#113242

Purpose

E. coli expression vector for untagged E. coli Cas1 + Cas2 with CRISPR array, leader tAggtag mutant

Depositor

Jennifer Doudna

Article

Wright et al Science. 2017 Sep 15;357(6356):1113-1118

Inserts

Cas1
Cas2
Leader-repeat

Expression

Bacterial

Mutation

TTGGTAG->tAggtag

Available Since

April 16, 2019

Availability

Academic Institutions and Nonprofits only

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pAW227

Plasmid

#113241

Purpose

E. coli expression vector for untagged E. coli Cas1 + Cas2 with CRISPR array, leader Atggtag mutant

Depositor

Jennifer Doudna

Article

Wright et al Science. 2017 Sep 15;357(6356):1113-1118

Inserts

Cas1
Cas2
Leader-repeat

Expression

Bacterial

Mutation

TTGGTAG->Atggtag

Available Since

April 16, 2019

Availability

Academic Institutions and Nonprofits only

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pAW207

Plasmid

#113247

Purpose

E. coli expression vector for IHFa K20A + IHFb

Depositor

Jennifer Doudna

Article

Wright et al Science. 2017 Sep 15;357(6356):1113-1118

Inserts

IHFa K20A (ihfA E. coli)
IHFb

Expression

Bacterial

Mutation

K20A

Promoter

T7

Available Since

Sept. 5, 2018

Availability

Academic Institutions and Nonprofits only

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pAW205

Plasmid

#113245

Purpose

E. coli expression vector for IHFa D14A + IHFb

Depositor

Jennifer Doudna

Article

Wright et al Science. 2017 Sep 15;357(6356):1113-1118

Inserts

IHFa D14A (ihfA E. coli)
IHFb

Expression

Bacterial

Mutation

D14A

Available Since

Sept. 5, 2018

Availability

Academic Institutions and Nonprofits only

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pAW217

Plasmid

#113239

Purpose

E. coli expression vector for untagged E. coli Cas1 + Cas2 with CRISPR array, leader ggtaC mutant

Depositor

Jennifer Doudna

Article

Wright et al Science. 2017 Sep 15;357(6356):1113-1118

Inserts

Cas1
Cas2
Leader-repeat

Expression

Bacterial

Mutation

GGTAG->ggtaC

Available Since

Aug. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pAW215

Plasmid

#113237

Purpose

E. coli expression vector for untagged E. coli Cas1 + Cas2 with CRISPR array, leader ggAag mutant

Depositor

Jennifer Doudna

Article

Wright et al Science. 2017 Sep 15;357(6356):1113-1118

Inserts

Cas1
Cas2
Leader-repeat

Expression

Bacterial

Mutation

GGTAG->ggAag

Available Since

Aug. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pAW214

Plasmid

#113236

Purpose

E. coli expression vector for untagged E. coli Cas1 + Cas2 with CRISPR array, leader gCtag mutant

Depositor

Jennifer Doudna

Article

Wright et al Science. 2017 Sep 15;357(6356):1113-1118

Inserts

Cas1
Cas2
Leader-repeat

Expression

Bacterial

Mutation

GGTAG->gCtag

Available Since

Aug. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pAW213

Plasmid

#113235

Purpose

E. coli expression vector for untagged E. coli Cas1 + Cas2 with CRISPR array, leader Cgtag mutant

Depositor

Jennifer Doudna

Article

Wright et al Science. 2017 Sep 15;357(6356):1113-1118

Inserts

Cas1
Cas2
Leader-repeat

Expression

Bacterial

Mutation

GGTAG->Cgtag

Available Since

Aug. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pAW211

Plasmid

#113233

Purpose

E. coli expression vector for untagged E. coli Cas1 + Cas2 with CRISPR array, leader GGTAG->AACGA

Depositor

Jennifer Doudna

Article

Wright et al Science. 2017 Sep 15;357(6356):1113-1118

Inserts

Cas1
Cas2
Leader-repeat

Expression

Bacterial

Mutation

GGTAG->AACGA

Available Since

Aug. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pAW210

Plasmid

#113189

Purpose

E. coli expression vector for untagged E. coli Cas1 H208A + Cas2 + CRISPR array

Depositor

Jennifer Doudna

Article

Wright et al Science. 2017 Sep 15;357(6356):1113-1118

Inserts

Cas1
Cas2
Leader-repeat

Expression

Bacterial

Mutation

H208A

Available Since

Aug. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pAW216

Plasmid

#113238

Purpose

E. coli expression vector for untagged E. coli Cas1 + Cas2 with CRISPR array, leader ggtTg mutant

Depositor

Jennifer Doudna

Article

Wright et al Science. 2017 Sep 15;357(6356):1113-1118

Inserts

Cas1
Cas2
Leader-repeat

Expression

Bacterial

Mutation

GGTAG->ggtTg

Available Since

Aug. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pAW203

Plasmid

#113243

Purpose

E. coli expression vector for IHFa E10A + IHFb

Depositor

Jennifer Doudna

Article

Wright et al Science. 2017 Sep 15;357(6356):1113-1118

Inserts

IHFa E10A (ihfA E. coli)
IHFb

Expression

Bacterial

Mutation

E10A

Available Since

Aug. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3-TfR-OVA

Plasmid

#64600

Purpose

Expression of chicken ovalbumin fused to the transmembrane domain of transferrin receptor as a model antigen for study of MHC II presentation

Depositor

Sandra Diebold, Martin Zenke

Article

Diebold et al Gene Ther. 2001 Mar;8(6):487-93.

Insert

Ovalbumin

Tags

Transmembrane domain of Transferrin Receptor (TfR…

Expression

Mammalian

Promoter

CMV

Available Since

July 21, 2015

Availability

Academic Institutions and Nonprofits only

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pBSV2G_PflaB-msfGFPBb

Plasmid

#118231

Purpose

Gentamicin-resistant E.coli/B. burgdorferi shuttle vector; Encodes flagellin promoter driven msfGFP

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2018 Oct 12. pii

Insert

flagellin promoter driven msfGFP

Expression

Bacterial

Available Since

Nov. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3-10His-mStayGold (E138D)

Plasmid

#211363

Purpose

Mammalian expression of the bright and photostable monomeric StayGold fluorescent protein (E138D). Contains a N-terminal 10xHis tag.

Depositor

Mohan Balasubramanian

Article

Ivorra-Molla et al Nat Biotechnol. 2023 Dec 11. doi: 10.103

Insert

mStayGold

Tags

10xHis-tag

Expression

Mammalian

Mutation

E138D

Promoter

CMV

Available Since

Dec. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pBS130

Plasmid

#26290

Depositor

Tom Clandinin

Article

Gohl et al Nat Methods. 2011 Mar;8(3):231-7. doi: 1

Insert

phiC31 integrase (int Streptomyces phage phiC31)

Use

Drosophila transgenesis

Expression

Bacterial and Insect

Available Since

Feb. 2, 2011

Availability

Academic Institutions and Nonprofits only

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