Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

We narrowed to 7,655 results for: Pol

Clear keywords
Showing: 2901 - 2920 of 7655 results

  2. pDB036d

    Plasmid
    #99216
    Purpose
    Accessory plasmid for PACE of MjTyrRS variants; negative selection
    Depositor
    David Liu
    Publication
    Bryson et al Nat Chem Biol. 2017 Oct 16. doi: 10.1038
    Insert
    Ppsp [SD8] gIII; PproK tyrT(Opt,CUA); PproA [SD4] T7RNAP(S12*,S203*)
    Expression
    Bacterial
    Mutation
    amber codons at positions 12 and 203 of T7 RNA po…
    Promoter
    Ppsp, PproK, and PproA
    Available since
    Nov. 20, 2017
    Availability
    Academic Institutions and Nonprofits only
    99216-plasmid-map-sequence-id-198818

    Enlarge

  4. NR1D2

    Plasmid
    #25186
    Purpose
    Baculovirus expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    NR1D2_07 (NR1D2 Human)
    Use
    Baculovirus expression
    Tags
    His
    Promoter
    polyhedrin
    Available since
    Aug. 24, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  6. p404GALS

    Plasmid
    #17435
    Depositor
    Nicolas Buchler, Fred Cross
    Insert
    GALS promoter + polylinker + CYC1 terminator (GAL1 Budding Yeast)
    Expression
    Bacterial and Yeast
    Mutation
    GALS promoter is between SacI and XbaI restrictio…
    Available since
    March 12, 2008
    Availability
    Academic Institutions and Nonprofits only
    17435-plasmid-map-sequence-id-8609

    Enlarge

  8. pYTSG

    Plasmid
    #159419
    Purpose
    Expression of proteins with a C-terminal thermostable GFP (TGP) tag in Saccharomyces cerevisiae
    Depositor
    Dianfan Li
    Publication
    Cai et al Commun Biol. 2020 Dec 10;3(1):753. doi:
    Insert
    thermostable GFP (TGP)
    Tags
    polyhistidine (poly-His)
    Expression
    Yeast
    Available since
    Jan. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    159419-plasmid-map-sequence-id-321534

    Enlarge

  14. pDule-IBBN (G2)

    Plasmid
    #85500
    Purpose
    Plasmid for incorporating the non-canonical amino acid IBBN with the Mj IBBN (G2) synthetase and cognate amber suppressing tRNA in Ecoli.
    Depositor
    Ryan Mehl
    Publication
    Peeler et al J Am Chem Soc. 2010 Oct 6;132(39):13575-
    Insert
    IBBN (G2) synthetase and cognate amber suppressing tRNA derived from M. jannaschii Tyrosine synthetase/tRNA system
    Expression
    Bacterial
    Mutation
    Y32G L65E F108W Q109M D158S R162K
    Promoter
    lpp (constitutive)
    Available since
    March 2, 2017
    Availability
    Academic Institutions and Nonprofits only
    85500-plasmid-map-sequence-id-169863

    Enlarge

  15. pGEM-cytoBirA-2A-mCherry-SV40pA-FKF

    Plasmid
    #79887
    Purpose
    Donor cassette containing HA-tagged biotin ligase (BirA), a Thosea asigna virus peptide (2A), mCherry protein and SV40 polyA tail, followed by FRT site-flanked Kanamycin selection gene
    Depositor
    Tatjana Sauka-Spengler
    Publication
    Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi
    Insert
    HA-tagged BirA, 2A, mCherry protein and SV40 polyA, followed by FRT site-flanked Kanamycin selection gene
    Expression
    Bacterial
    Available since
    Oct. 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    79887-plasmid-map-sequence-id-402553

    Enlarge

  16. pEMS1062

    Plasmid
    #29011
    Purpose
    The plasmid contains the Pleaides (Ple) MiniPromoter Ple155 derived from the human PCP2 gene and designed for expression in ON Bipolar cells of the retina.
    Depositor
    Elizabeth Simpson
    Publication
    Korecki et al Gene Ther. 2021 Feb 2. pii: 10.1038/s414
    Insert
    Ple155-Cre/EGFP-NLS
    Use
    High copy number homologous recombination plasmid…
    Expression
    Mammalian
    Promoter
    MiniPromoter Ple155 from the human PCP2 gene
    Available since
    Feb. 22, 2012
    Availability
    Academic Institutions and Nonprofits only
    29011-plasmid-map-sequence-id-13417

    Enlarge

  19. pGEX-2T-RNAPII-CTD wt #14-HA

    Plasmid
    #160688
    Purpose
    Expression of GST-RNA Polymerase II C-terminal domain fusion protein. Contains 14 of the consensus heptapeptide repeats shown to be phosphorylated by Cdc2 kinase.
    Depositor
    Jeffry Corden
    Publication
    Patturajan et al J Biol Chem. 1998 Feb 20;273(8):4689-94.
    Insert
    RNA Polymerase II CTD
    Expression
    Bacterial
    Mutation
    C terminal domain only
    Available since
    Nov. 19, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    160688-plasmid-map-sequence-id-317773

    Enlarge

  20. pHB-2

    Plasmid
    #140955
    Purpose
    For PHB production in E. coli (T7-pAC mutant). Please note that this is listed as #140954 in the publication.
    Depositor
    Kang Zhou
    Publication
    Liu et al Metab Eng. 2020 Sep;61:206-214. doi: 10.
    Insert
    phaCAB
    Expression
    Bacterial
    Mutation
    R238S mutation in PhaC
    Available since
    April 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    140955-plasmid-map-sequence-id-334168

    Enlarge

Showing: 2901 - 2920 of 7655 results