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SARS-CoV-2 Mpro protease (C145S mutant - catalytically inactive); aliases: 3CLpro, 3C-like

Plasmid

#204769

Purpose

Bacterial expression of Sars Cov 2 MPRO C145S mutant

Depositor

ASAP Discovery, Frank von Delft

Insert

Sars Cov 2 MPRO (ORF1ab Sars Cov 2)

Expression

Bacterial

Mutation

C145S mutant

Available Since

Nov. 30, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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JPUB_006820

Plasmid

#87921

Purpose

E. coli expression of K. stuttgartiensis protein

Depositor

Harry Beller

Article

Javidpour et al PLoS One. 2016 Mar 14;11(3):e0151087. do

Insert

Kuste2803

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

April 4, 2017

Availability

Academic Institutions and Nonprofits only

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JPUB_006826

Plasmid

#87923

Purpose

E. coli expression of K. stuttgartiensis protein

Depositor

Harry Beller

Article

Javidpour et al PLoS One. 2016 Mar 14;11(3):e0151087. do

Insert

Kuste3608

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

April 4, 2017

Availability

Academic Institutions and Nonprofits only

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pQuantEGFP-hygro-kana

Plasmid

#45588

Depositor

Andrzej Dziembowski

Article

Orlowska et al Nucleic Acids Res. 2013 Sep;41(17):e167.

Type

Empty backbone

Use

Cre/Lox; Dt40 cell line targeting

Tags

Quant-EGFP tag

Available Since

July 8, 2013

Availability

Academic Institutions and Nonprofits only

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pCis mCherry-SS Himar1 A7

Plasmid

#84687

Purpose

Can be used for the transformation of rickettsia, anaplasma and ehrlichia. Uses the Himar1 transposon system, with the A7 allele and both transposase and transposon contained in one plasmid.

Depositor

Ulrike Munderloh

Article

Munderloh et al Intracellular Pathogens II: Rickettsiale

Type

Empty backbone

Expression

Bacterial

Available Since

Nov. 7, 2016

Availability

Academic Institutions and Nonprofits only

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pCis GFPuv-SS Himar1 A7

Plasmid

#84686

Purpose

Can be used for the transformation of rickettsia, anaplasma and ehrlichia. Uses the Himar1 transposon system, with the A7 allele and both transposase and transposon contained in one plasmid.

Depositor

Ulrike Munderloh

Article

Munderloh et al Intracellular Pathogens II: Rickettsiale

Type

Empty backbone

Expression

Bacterial

Available Since

Nov. 1, 2016

Availability

Academic Institutions and Nonprofits only

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Ecoli cpGFP

Plasmid

#215713

Purpose

Expresses cpGFP in Ecoli

Depositor

Katharine Diehl

Article

Smith et al Cell Chem Biol. 2025 Jan 21:S2451-9456(2

Insert

cpGFP

Tags

6xHis

Expression

Bacterial

Mutation

circularly permuted GFP

Promoter

T7

Available Since

April 23, 2024

Availability

Academic Institutions and Nonprofits only

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pCC25

Plasmid

#177319

Purpose

Expression plasmid for production of recombinant T. terrestris CutA NTase fragment in E. coli

Depositor

Rafal Tomecki

Article

Tomecki et al RNA Biol. 2021 Nov 12;18(sup2):623-639.

Insert

TtCutA 240-603

Tags

6xHis-SUMOTag

Expression

Bacterial

Promoter

T7

Available Since

Jan. 28, 2022

Availability

Academic Institutions and Nonprofits only

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KRASB

Plasmid

#25153

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Cheryl Arrowsmith

Insert

KRASb:M1-K169 (KRAS Human)

Tags

His

Expression

Bacterial

Mutation

Q61H

Available Since

Aug. 6, 2010

Availability

Industry, Academic Institutions, and Nonprofits
pET:GACT:GFP

Plasmid

#60734

Purpose

A modified pET28 driving expression of GFP, biobrick part Bba_E0040. No LacO site downstream of PT7. T7 promoter has base pairs 'GACT' between -11 and -8.

Depositor

Matthew Bennett

Article

Shis et al Proc Natl Acad Sci U S A. 2013 Mar 26;11

Insert

GFP

Use

Synthetic Biology

Expression

Bacterial

Available Since

Nov. 5, 2014

Availability

Academic Institutions and Nonprofits only

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