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pUC57-mini v2-HF24_F19

Plasmid

#195733

Purpose

This plasmid carries a fragment from a 24-fragment lacI/lacZ cassette split that was designed as a Golden Gate assembly with high fidelity.

Depositor

Eric Cantor

Article

Potapov et al ACS Synth Biol. 2018 Nov 16;7(11):2665-2

Insert

Fragment#19 of 24 fragment splits in lacI/lacZ cassette

Use

Synthetic Biology

Available Since

Feb. 23, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pUC57-mini v2-HF24_F2

Plasmid

#195716

Purpose

This plasmid carries a fragment from a 24-fragment lacI/lacZ cassette split that was designed as a Golden Gate assembly with high fidelity.

Depositor

Eric Cantor

Article

Potapov et al ACS Synth Biol. 2018 Nov 16;7(11):2665-2

Insert

Fragment#2 of 24 fragment splits in lacI/lacZ cassette

Use

Synthetic Biology

Available Since

Feb. 23, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pUC57-mini v2-HF24_F11

Plasmid

#195725

Purpose

This plasmid carries a fragment from a 24-fragment lacI/lacZ cassette split that was designed as a Golden Gate assembly with high fidelity.

Depositor

Eric Cantor

Article

Potapov et al ACS Synth Biol. 2018 Nov 16;7(11):2665-2

Insert

Fragment#11 of 24 fragment splits in lacI/lacZ cassette

Use

Synthetic Biology

Available Since

Feb. 23, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pUC57-mini v2-HF24_F16

Plasmid

#195730

Purpose

This plasmid carries a fragment from a 24-fragment lacI/lacZ cassette split that was designed as a Golden Gate assembly with high fidelity.

Depositor

Eric Cantor

Article

Potapov et al ACS Synth Biol. 2018 Nov 16;7(11):2665-2

Insert

Fragment#16 of 24 fragment splits in lacI/lacZ cassette

Use

Synthetic Biology

Available Since

Feb. 23, 2023

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pUC57-mini v2-HF24_F18

Plasmid

#195732

Purpose

This plasmid carries a fragment from a 24-fragment lacI/lacZ cassette split that was designed as a Golden Gate assembly with high fidelity.

Depositor

Eric Cantor

Article

Potapov et al ACS Synth Biol. 2018 Nov 16;7(11):2665-2

Insert

Fragment#18 of 24 fragment splits in lacI/lacZ cassette

Use

Synthetic Biology

Available Since

Feb. 23, 2023

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pUC57-mini v2-HF24_F1

Plasmid

#195715

Purpose

This plasmid carries a fragment from a 24-fragment lacI/lacZ cassette split that was designed as a Golden Gate assembly with high fidelity.

Depositor

Eric Cantor

Article

Potapov et al ACS Synth Biol. 2018 Nov 16;7(11):2665-2

Insert

Fragment#1 of a 24 fragment split in lacI/lacZ cassette

Use

Synthetic Biology

Available Since

Feb. 23, 2023

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pUC57-mini v2-HF24_F9

Plasmid

#195723

Purpose

This plasmid carries a fragment from a 24-fragment lacI/lacZ cassette split that was designed as a Golden Gate assembly with high fidelity.

Depositor

Eric Cantor

Article

Potapov et al ACS Synth Biol. 2018 Nov 16;7(11):2665-2

Insert

Fragment#9 of 24 fragment splits in lacI/lacZ cassette

Use

Synthetic Biology

Available Since

Feb. 23, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pUC57-mini v2-HF24_F23

Plasmid

#195737

Purpose

This plasmid carries a fragment from a 24-fragment lacI/lacZ cassette split that was designed as a Golden Gate assembly with high fidelity.

Depositor

Eric Cantor

Article

Potapov et al ACS Synth Biol. 2018 Nov 16;7(11):2665-2

Insert

Fragment#23 of 24 fragment splits in lacI/lacZ cassette

Use

Synthetic Biology

Available Since

Feb. 23, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pUC57-mini v2-HF24_F21

Plasmid

#195735

Purpose

This plasmid carries a fragment from a 24-fragment lacI/lacZ cassette split that was designed as a Golden Gate assembly with high fidelity.

Depositor

Eric Cantor

Article

Potapov et al ACS Synth Biol. 2018 Nov 16;7(11):2665-2

Insert

Fragment#21 of 24 fragment splits in lacI/lacZ cassette

Use

Synthetic Biology

Available Since

Feb. 23, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pX330-MGAT1-KO

Plasmid

#80009

Purpose

gRNA to knock out expression of MGAT1 gene. The product of this gene is essential for synthesis of complex and hybrid N-Glycans.

Depositor

Sriram Neelamegham

Article

Stolfa et al Sci Rep. 2016 Jul 26;6:30392. doi: 10.10

Insert

MGAT1 (MGAT1 Human)

Use

CRISPR

Available Since

July 25, 2016

Availability

Academic Institutions and Nonprofits only

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pX330-COSMC-KO

Plasmid

#80008

Purpose

gRNA to knock out expression of COSMC (C1GalT1C1) gene. The product of this gene is a chaperone aiding in synthesis of Core1 structures on O-linked glycans.

Depositor

Sriram Neelamegham

Article

Stolfa et al Sci Rep. 2016 Jul 26;6:30392. doi: 10.10

Insert

COSMC (C1GALT1C1 Human)

Use

CRISPR

Available Since

July 25, 2016

Availability

Academic Institutions and Nonprofits only

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pLenti-dCas9-T2A-GFP

Plasmid

#193781

Purpose

Tricistronic expression of dCas9-EGFP-BSD

Depositor

James Eshleman

Article

Teh et al NAR Cancer. 2024 Jun 19;6(2):zcae028. do

Insert

dead Cas9

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Promoter

In frame with existing Cas9 promoter

Available Since

May 28, 2024

Availability

Academic Institutions and Nonprofits only

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CIP2Aprom108bp-pGL4.10Luc

Plasmid

#60875

Purpose

Luc reporter driven by 108 bp of the CIP2A promoter

Depositor

Jukka Westermarck

Article

Khanna et al PLoS One. 2011 Mar 22;6(3):e17979. doi:

Insert

NM_020890108 bp promoter fragment of Cancerous Inhibitor of PP2A.2 (CIP2A Human)

Use

Luciferase; Promoterless

Available Since

April 5, 2016

Availability

Academic Institutions and Nonprofits only
pLentiCas9-T2A-mApple

Plasmid

#193780

Purpose

Tricistronic expression of Cas9-mApple-BSD in mammalian cells

Depositor

James Eshleman

Article

Teh et al NAR Cancer. 2024 Jun 19;6(2):zcae028. do

Insert

mApple

Use

Lentiviral

Expression

Mammalian

Promoter

In frame with Cas9

Available Since

May 28, 2024

Availability

Academic Institutions and Nonprofits only

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CIP2Aprom1802bp-pGL4.10Luc

Plasmid

#60868

Purpose

Luc reporter driven by 1802 bp of the CIP2A promoter

Depositor

Jukka Westermarck

Article

Khanna et al PLoS One. 2011 Mar 22;6(3):e17979. doi:

Insert

1802 bp promoter fragment of Cancerous Inhibitor of PP2A (CIP2A Human)

Use

Luciferase; Promoterless

Available Since

April 5, 2016

Availability

Academic Institutions and Nonprofits only

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CIP2Aprom285bp-pGL4.10Luc

Plasmid

#60873

Purpose

Luc reporter driven by 285 bp of the CIP2A promoter

Depositor

Jukka Westermarck

Article

Khanna et al PLoS One. 2011 Mar 22;6(3):e17979. doi:

Insert

285 bp promoter fragment of Cancerous Inhibitor of PP2A2 (CIP2A Human)

Use

Luciferase; Promoterless

Available Since

April 5, 2016

Availability

Academic Institutions and Nonprofits only
pINDUCER20-hGfi1

Plasmid

#121200

Purpose

inducible expression of hGfi1 in colorectal cancer cells

Depositor

Noah Shroyer

Article

Chen et al Mol Cancer Res. 2019 Mar;17(3):697-708.

Insert

Growth factor independent 1 transcriptional (GFI1 Human)

Use

Lentiviral

Expression

Mammalian

Mutation

P2L

Available Since

July 25, 2025

Availability

Academic Institutions and Nonprofits only

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pLJC2-DHFR-3xFLAG

Plasmid

#217428

Purpose

Lentiviral overexpression of human DHFR

Depositor

Jason Cantor

Article

Flickinger et al Sci Adv. 2024 Oct 4;10(40):eadq3591. doi

Insert

DHFR (DHFR Human)

Use

Lentiviral

Tags

3xFLAG

Expression

Mammalian

Mutation

Insert sequence is a codon-optimized gBLOCK (IDT)

Promoter

CMV

Available Since

Jan. 31, 2025

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPR-v1-sgABCC4_1

Plasmid

#217433

Purpose

Lentiviral vector expressing Cas9 and an sgRNA targeting human ABCC4

Depositor

Jason Cantor

Article

Flickinger et al Sci Adv. 2024 Oct 4;10(40):eadq3591. doi

Insert

sgRNA 1 targeting ABCC4 (ABCC4 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Jan. 31, 2025

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPR-v1-sgABCC4_3

Plasmid

#217434

Purpose

Lentiviral vector expressing Cas9 and an sgRNA targeting human ABCC4

Depositor

Jason Cantor

Article

Flickinger et al Sci Adv. 2024 Oct 4;10(40):eadq3591. doi

Insert

sgRNA 3 targeting ABCC4 (ABCC4 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Jan. 31, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge