Addgene

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pBS-F1-ACG

Plasmid

#30688

Depositor

Nathan Lawson, Scot Wolfe

Article

Zhu et al Development. 2011 Oct;138(20):4555-64.

Insert

F1 ACG

Use

Cloning vector

Tags

Expression

Mutation

Promoter

Available since

Nov. 9, 2011

Availability

Academic Institutions and Nonprofits only
pBS-F1-AGG

Plasmid

#30689

Depositor

Nathan Lawson, Scot Wolfe

Article

Zhu et al Development. 2011 Oct;138(20):4555-64.

Insert

F1 AGG

Use

Cloning vector

Tags

Expression

Mutation

Promoter

Available since

Nov. 9, 2011

Availability

Academic Institutions and Nonprofits only
pBS-F1-ATG

Plasmid

#30690

Depositor

Nathan Lawson, Scot Wolfe

Article

Zhu et al Development. 2011 Oct;138(20):4555-64.

Insert

F1 ATG

Use

Cloning vector

Tags

Expression

Mutation

Promoter

Available since

Nov. 9, 2011

Availability

Academic Institutions and Nonprofits only
pBS-F1-CGG

Plasmid

#30692

Depositor

Nathan Lawson, Scot Wolfe

Article

Zhu et al Development. 2011 Oct;138(20):4555-64.

Insert

F1 CGG

Use

Cloning vector

Tags

Expression

Mutation

Promoter

Available since

Nov. 9, 2011

Availability

Academic Institutions and Nonprofits only
pBS-F1-GAA

Plasmid

#30693

Depositor

Nathan Lawson, Scot Wolfe

Article

Zhu et al Development. 2011 Oct;138(20):4555-64.

Insert

F1 GAA

Use

Cloning vector

Tags

Expression

Mutation

Promoter

Available since

Nov. 9, 2011

Availability

Academic Institutions and Nonprofits only
iXFGFR4r

Plasmid

#31262

Depositor

Masahiro Yamagishi

Article

Yamagishi et al Int J Dev Biol. 2010 . 54(1):93-104.

Insert

Xenopus FGFR4-ICD-FRB (fgfr4-a Frog)

Use

Tags

HA epitope tag and myristoylation signal

Expression

Bacterial and Mammalian

Mutation

Promoter

Available since

Sept. 22, 2011

Availability

Academic Institutions and Nonprofits only
pLNC Int3HA

Plasmid

#21329

Depositor

Jan Kitajewski

Article

Uyttendaele et al Dev Biol. 1998 Apr 15. 196(2):204-17.

Insert

Int3 (Notch4 Mouse)

Use

Retroviral

Tags

HA

Expression

Mutation

Promoter

Available since

Sept. 11, 2009

Availability

Academic Institutions and Nonprofits only
LMB

Plasmid

#12502

Depositor

David Baker

Article

Dantas et al J Mol Biol. 2003 Sep 12. 332(2):449-60.

Insert

LMB

Use

Tags

6xHis

Expression

Bacterial

Mutation

RosettaDesign was used to design sequences. Align…

Promoter

Available since

March 13, 2007

Availability

Academic Institutions and Nonprofits only
ACY

Plasmid

#12396

Depositor

David Baker

Article

Dantas et al J Mol Biol. 2003 Sep 12. 332(2):449-60.

Insert

ACY

Use

Tags

6xHis

Expression

Bacterial

Mutation

RosettaDesign was used to design sequences. Align…

Promoter

Available since

March 13, 2007

Availability

Academic Institutions and Nonprofits only
TEN

Plasmid

#12498

Depositor

David Baker

Article

Dantas et al J Mol Biol. 2003 Sep 12. 332(2):449-60.

Insert

TEN

Use

Tags

6xHis

Expression

Bacterial

Mutation

RosettaDesign was used to design sequences. Align…

Promoter

Available since

March 13, 2007

Availability

Academic Institutions and Nonprofits only
pGEX2T E7 del PTLHE

Plasmid

#13637

Depositor

Karl Munger

Article

Wu et al J Virol. 1993 Apr . 67(4):2402-7.

Insert

HPV 16 E7 del PTLHE (E7 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

del aa 6-10

Promoter

Available since

March 2, 2007

Availability

Academic Institutions and Nonprofits only
pMT FRB-Draper INT-mCherry

Plasmid

#126666

Purpose

Inducible expression of a synthetic FRB-Draper receptor in S2 cells

Depositor

Ron Vale

Article

Williamson et al J Cell Biol. 2018 Nov 5;217(11):3977-399

Insert

FRB-Draper INT domain mCherry

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Availability

Academic Institutions and Nonprofits only

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pLenti-puro

Plasmid

#39481

Purpose

Tetracycline inducible 3rd generation lentiviral vector

Depositor

Ie-Ming Shih

Article

Guan et al Cancer Res. 2011 Nov 1;71(21):6718-27. E

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

Lentiviral

Tags

V5 and 6XHis

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 13, 2012

Availability

Academic Institutions and Nonprofits only

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pMT-Bip-GFP:V5:KDEL

Plasmid

#69917

Purpose

An endoplasmic reticulum (ER) marker used to visulaize the ER structure in Drosophilia S2 cells.

Depositor

Kenneth Irvine

Article

Okajima et al Science. 2005 Mar 11;307(5715):1599-603.

Insert

GFP

Use

Tags

Drosophila BiP signal sequence, KDEL ER targeting…

Expression

Insect

Mutation

Promoter

pMT

Available since

Nov. 3, 2015

Availability

Academic Institutions and Nonprofits only

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LacZ_pHIVEGFP

Plasmid

#191251

Purpose

Expresses LacZ in mammalian cells (used as a control)

Depositor

Chen Davidovich

Article

Zhang et al Nat Commun. 2021 Jul 28;12(1):4592. doi:

Insert

LacZ

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

EF1alpha

Available since

Nov. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3 Tat HA (P#29)

Plasmid

#14654

Depositor

Matija Peterlin

Article

Cujec et al Genes Dev. 1997 Oct 15. 11(20):2645-57.

Insert

Tat (tat HIV)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

June 30, 2008

Availability

Academic Institutions and Nonprofits only

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pMpGWB402-er-mCherry

Plasmid

#186288

Purpose

Binary vector for the expression of er-mCherry (SP-mCherry-HDEL) in plants (for Agrobacterium-mediated genetic transformation and visualization of endoplasmic reticulum)

Depositor

Yutaka Kodama

Article

Ishikawa et al Plant J. 2022 Apr 27. doi: 10.1111/tpj.1

Insert

er-mCherry

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pTP1122

Plasmid

#104159

Purpose

Bacterial expression plasmid of anti-mouse IgG1 Fc nanobody TP1107 (3x Cysteines)

Depositor

Dirk Görlich

Article

Pleiner et al J Cell Biol. 2018 Mar 5;217(3):1143-1154

Insert

Anti-mouse IgG1 Fc specific nanobody TP1107 (3xCysteines)

Use

Tags

14xHistidine tag and NEDD8 from Brachypodium dist…

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 21, 2017

Availability

Academic Institutions and Nonprofits only

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pTP955

Plasmid

#104164

Purpose

Bacterial expression plasmid of anti-rabbit IgG Fc nanobody TP897 (3x Cysteines)

Depositor

Dirk Görlich

Article

Pleiner et al J Cell Biol. 2018 Mar 5;217(3):1143-1154

Insert

Anti-rabbit IgG Fc specific nanobody TP897 (3xCysteines)

Use

Tags

14xHistidine tag and NEDD8 from Brachypodium dist…

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 21, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
anxA1-GFP

Plasmid

#107194

Purpose

Expresses porcine annexin A1-GFP fusion protein for live cell imaging

Depositor

Ursula Rescher, Volker Gerke

Article

Rescher et al J Cell Sci. 2000 Nov;113 ( Pt 22):3931-8

Insert

annexin A1

Use

Tags

Green Fluorescent Protein

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 13, 2018

Availability

Academic Institutions and Nonprofits only

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GEARBOCS-SPARCL1-N-mCherryTag

Plasmid

#218186

Purpose

To tag Hevin with mCherry at the N-terminal

Depositor

Cagla Eroglu

Article

Bindu et al bioRxiv. 2023 Jan 19:2023.01.17.524433.

Insert

sgRNA (Sparcl1 Mouse)

Use

AAV and CRISPR

Tags

Expression

Mutation

Promoter

Available since

Aug. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA4-GFP-Vinculin

Plasmid

#107153

Purpose

mammalian expression

Depositor

Tina Izard

Article

Chinthalapudi et al J Cell Biol. 2014 Dec 8;207(5):643-56. d

Insert

Vinculin (VCL Human)

Use

Tags

(His)10-GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 8, 2018

Availability

Academic Institutions and Nonprofits only

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Myc-hZO-1-HA

Plasmid

#236528

Purpose

For expression of ZO-1 (human) in mammalian cells

Depositor

Sandra Citi

Article

Spadaro et al Curr Biol. 2017 Dec 18;27(24):3783-3795.

Insert

TJP1 (TJP1 Human)

Use

Tags

HA and Myc

Expression

Mammalian

Mutation

WT

Promoter

Available since

April 16, 2025

Availability

Academic Institutions and Nonprofits only

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pLEM415-ldhL-mRFP1

Plasmid

#99842

Purpose

Expression of RFP in Lactobacillus

Depositor

Sujin Bao

Article

Bao et al PLoS One. 2013;8(3):e60007. doi: 10.1371

Insert

monomeric red fluorescent protein

Use

Tags

FLAG

Expression

Bacterial

Mutation

Promoter

ldhl

Available since

Nov. 13, 2017

Availability

Academic Institutions and Nonprofits only

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EGFP-alphasynuclein-WT

Plasmid

#40822

Depositor

David Rubinsztein

Article

Furlong et al Biochem J. 2000 Mar 15;346 Pt 3:577-81.

Insert

SNCA (SNCA Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 8, 2013

Availability

Academic Institutions and Nonprofits only

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3xAP1pGL3 (3xAP-1 in pGL3-basic)

Plasmid

#40342

Depositor

Alexander Dent

Article

Vasanwala et al J Immunol. 2002 Aug 15;169(4):1922-9.

Insert

3xAP-1

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Mutation

Contains three canonical AP-1 binding sites (TGAC…

Promoter

Available since

Sept. 28, 2012

Availability

Academic Institutions and Nonprofits only

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pCMV-8xHis-Ub

Plasmid

#107392

Purpose

Mammalian expression 8xHis-Ub

Depositor

William Kaelin

Article

Lu et al Science. 2014 Jan 17;343(6168):305-9. do

Insert

8 tandem repeats of 6xHis-Ub (See comments below)

Use

Tags

None

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 19, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
p23-DDX21 WT

Plasmid

#128803

Purpose

overexpression of DDX21 in human cells

Depositor

Ling-Ling Chen

Article

Xing et al Cell. 2017 May 4;169(4):664-678.e16. doi

Insert

DDX21 WT

Use

Tags

flag

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pEvoltac EcW-TGA-h9

Plasmid

#218765

Purpose

encodes EcWRS-h9 and EcW-tRNA-TGA

Depositor

Abhishek Chatterjee

Article

Italia et al Nat Chem Biol. 2017 Apr;13(4):446-450. d

Insert

EcWRS-h9

Use

Tags

Expression

Bacterial

Mutation

S8A, V144S, V146A

Promoter

tacI

Available since

June 21, 2024

Availability

Academic Institutions and Nonprofits only

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pUdO1k

Plasmid

#210234

Purpose

high-copy plasmid for bacterial expression

Depositor

François-Xavier Campbell-Valois

Article

Manigat et al ACS Synth Biol. 2024 Jan 18. doi: 10.102

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 16, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge