Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

We narrowed to 7,580 results for: Ski;

Showing: 5881 - 5920 of 7580 results

Results per page:

Sort results by:

Flag-Src C185A H201A H492A E524A

Plasmid

#246427

Purpose

Expresses Src pH insensitive mutant in Mammalian cells

Depositor

Katharine White

Article

Van Dyck et al Sci Signal. 2025 Nov 11;18(912):eadt3018

Insert

Src (SRC Human)

Tags

Flag

Expression

Mammalian

Mutation

changed C188 to A, H204 to A, H495 to A, and E527…

Available Since

Nov. 12, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pLV-FAM136A-WT-3xFLAG-hygro

Plasmid

#244880

Purpose

Overexpression of wild-type human FAM136A

Depositor

Alexis Jourdain

Article

Harhai et al Cell Rep. 2025 Jul 25;44(8):116069. doi:

Insert

FAM136A (FAM136A Human)

Use

Lentiviral

Tags

3xFLAG

Expression

Mammalian

Promoter

EF1a

Available Since

Oct. 15, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pLentiCRISPR V2 CLUH sg1

Plasmid

#244857

Purpose

Knockout of human CLUH

Depositor

Alexis Jourdain

Article

Harhai et al Cell Rep. 2025 Jul 25;44(8):116069. doi:

Insert

CLUH sgRNA (CLUH Human)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

Oct. 15, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pLentiCRISPR V2 ALAD_sg2

Plasmid

#244856

Purpose

Knockout of human ALAD

Depositor

Alexis Jourdain

Article

Harhai et al Cell Rep. 2025 Jul 25;44(8):116069. doi:

Insert

ALAD sgRNA (ALAD Human)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

Oct. 15, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pLentiCRISPR V2 ALAD_sg1

Plasmid

#244855

Purpose

Knockout of human ALAD

Depositor

Alexis Jourdain

Article

Harhai et al Cell Rep. 2025 Jul 25;44(8):116069. doi:

Insert

ALAD sgRNA (ALAD Human)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

Oct. 15, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pLentiCRISPR V2 CLUH sg2

Plasmid

#244858

Purpose

Knockout of human CLUH

Depositor

Alexis Jourdain

Article

Harhai et al Cell Rep. 2025 Jul 25;44(8):116069. doi:

Insert

CLUH sgRNA (CLUH Human)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

Oct. 15, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pLentiCRISPR V2 HMBS_sg2

Plasmid

#244860

Purpose

Knockout of human HMBS

Depositor

Alexis Jourdain

Article

Harhai et al Cell Rep. 2025 Jul 25;44(8):116069. doi:

Insert

HMBS sgRNA (HMBS Human)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

Oct. 15, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pLentiCRISPR V2 HMBS_sg1

Plasmid

#244859

Purpose

Knockout of human HMBS

Depositor

Alexis Jourdain

Article

Harhai et al Cell Rep. 2025 Jul 25;44(8):116069. doi:

Insert

HMBS sgRNA (HMBS Human)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

Oct. 15, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pLentiCRISPR IMMP1L sg2

Plasmid

#244850

Purpose

Knockout of human IMMP1L

Depositor

Alexis Jourdain

Article

Harhai et al Cell Rep. 2025 Jul 25;44(8):116069. doi:

Insert

IMMP1L sgRNA (IMMP1L Human)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

Oct. 15, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pLentiCRISPR IMMP1L sg1

Plasmid

#244849

Purpose

Knockout of human IMMP1L

Depositor

Alexis Jourdain

Article

Harhai et al Cell Rep. 2025 Jul 25;44(8):116069. doi:

Insert

IMMP1L sgRNA (IMMP1L Human)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

Oct. 15, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-hsynapsin-DIO-scramble shRNA - mCherry

Plasmid

#245064

Purpose

AAV transgene designes to express scramble (control) shRNA sequence cell-type selectively. Scramble hairpin expressed from the mir30 cassette together with mCherry.

Depositor

William Wisden

Article

Anuncibay Soto et al Proc Natl Acad Sci U S A. 2025 Oct 14;12

Insert

AAV-DIO-scramble-shRNA-mCherry

Use

AAV, Cre/Lox, and RNAi

Tags

mCherry

Expression

Mammalian

Promoter

human synapsin

Available Since

Oct. 15, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
GfaABC1D-flag-mAdrb1-WPRE

Plasmid

#223671

Purpose

AAV expression of flag-mAdrb1 from GfaABC1D promoter

Depositor

Jun Nagai

Article

Dewa et al Nature. 2025 Oct 15. doi: 10.1038/s41586

Insert

mAdrb1 (Adrb1 Mouse)

Use

AAV

Tags

FLAG

Promoter

GfaABC1D

Available Since

Oct. 15, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
GfaABC1D-flag-mAdrb1-mNeonGreen-WPRE

Plasmid

#223672

Purpose

AAV expression of flag-mAdrb1-mNeonGreen from GfaABC1D promoter

Depositor

Jun Nagai

Article

Dewa et al Nature. 2025 Oct 15. doi: 10.1038/s41586

Insert

mAdrb1-mNeonGreen (Adrb1 Mouse)

Use

AAV

Tags

FLAG and mNeonGreen

Promoter

GfaABC1D

Available Since

Oct. 15, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEX-4T1 GST-SHP2 H116A

Plasmid

#244492

Purpose

Bacterial Expression of SHP2 mutant

Depositor

Katharine White

Article

Van Dyck et al Sci Signal. 2025 Nov 11;18(912):eadt3018

Insert

PTPN11 (PTPN11 Human)

Tags

GST

Expression

Bacterial

Mutation

changed Histidine 116 to Alanine

Available Since

Oct. 2, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEX-4T1 SHP2 E252A

Plasmid

#244491

Purpose

Bacterial Expression of SHP2 mutant

Depositor

Katharine White

Article

Van Dyck et al Sci Signal. 2025 Nov 11;18(912):eadt3018

Insert

PTPN11 (PTPN11 Human)

Tags

GST

Expression

Bacterial

Mutation

changed Glutamate 252 to Alanine

Available Since

Oct. 2, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pDMJ2-SARS-CoV-2-Spike (B.1-4b)

Plasmid

#241044

Purpose

Expresses SARS-CoV-2 spike (B.1-4b) protein for creating pseudotyped virus

Depositor

Jeremy Luban

Article

Youssef et al Immunity. 2025 May 6:S1074-7613(25)00178

Insert

Spike (B.1-4b) (S SARS-CoV-2)

Expression

Mammalian

Mutation

K147N,S494R,K529L,D614G,R683L

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pDMJ2-SARS-CoV-2-Spike (B.1-4c)

Plasmid

#241045

Purpose

Expresses SARS-CoV-2 spike (B.1-4c) protein for creating pseudotyped virus

Depositor

Jeremy Luban

Article

Youssef et al Immunity. 2025 May 6:S1074-7613(25)00178

Insert

Spike (B.1-4c) (S SARS-CoV-2)

Expression

Mammalian

Mutation

N439R,T478K,E484V,D614G,K529L

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pDMJ2-SARS-CoV-2-Spike (B.1-4a)

Plasmid

#241042

Purpose

Expresses SARS-CoV-2 spike (B.1-4a) protein for creating pseudotyped virus

Depositor

Jeremy Luban

Article

Youssef et al Immunity. 2025 May 6:S1074-7613(25)00178

Insert

Spike (B.1-4a) (S SARS-CoV-2)

Expression

Mammalian

Mutation

K147N,S494R,F490R,D614G,R683N

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pDMJ2-SARS-CoV-2-Spike (B.1-4d)

Plasmid

#241043

Purpose

Expresses SARS-CoV-2 spike (B.1-4d) protein for creating pseudotyped virus

Depositor

Jeremy Luban

Article

Youssef et al Immunity. 2025 May 6:S1074-7613(25)00178

Insert

Spike (B.1-4d) (S SARS-CoV-2)

Expression

Mammalian

Mutation

T76I,M153S,Y473F,Q493R,D614G

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pDMJ2-SARS-CoV-2-Spike (B.1-2c)

Plasmid

#241040

Purpose

Expresses SARS-CoV-2 spike (B.1-2c) protein for creating pseudotyped virus

Depositor

Jeremy Luban

Article

Youssef et al Immunity. 2025 May 6:S1074-7613(25)00178

Insert

Spike (B.1-2c) (S SARS-CoV-2)

Expression

Mammalian

Mutation

R346T,K417V,D614G

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pDMJ2-SARS-CoV-2-Spike (B.1-2d)

Plasmid

#241041

Purpose

Expresses SARS-CoV-2 spike (B.1-2d) protein for creating pseudotyped virus

Depositor

Jeremy Luban

Article

Youssef et al Immunity. 2025 May 6:S1074-7613(25)00178

Insert

Spike (B.1-2d) (S SARS-CoV-2)

Expression

Mammalian

Mutation

K147N,K417V,D614G

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pDMJ2-SARS-CoV-2-Spike (B.1-2a)

Plasmid

#241038

Purpose

Expresses SARS-CoV-2 spike (B.1-2a) protein for creating pseudotyped virus

Depositor

Jeremy Luban

Article

Youssef et al Immunity. 2025 May 6:S1074-7613(25)00178

Insert

Spike (B.1-2a) (S SARS-CoV-2)

Expression

Mammalian

Mutation

T478K,R346T,D614G

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pDMJ2-SARS-CoV-2-Spike (B.1-2b)

Plasmid

#241039

Purpose

Expresses SARS-CoV-2 spike (B.1-2b) protein for creating pseudotyped virus

Depositor

Jeremy Luban

Article

Youssef et al Immunity. 2025 May 6:S1074-7613(25)00178

Insert

Spike (B.1-2b) (S SARS-CoV-2)

Expression

Mammalian

Mutation

R346T,S494R,D614G

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pDMJ2-SARS-CoV-2-Spike (B.1)

Plasmid

#241033

Purpose

Expresses SARS-CoV-2 spike (B.1) protein for creating pseudotyped virus

Depositor

Jeremy Luban

Article

Youssef et al Immunity. 2025 May 6:S1074-7613(25)00178

Insert

Spike (B.1) (S SARS-CoV-2)

Expression

Mammalian

Mutation

D614G

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1-Ub-Myc-LgBiT

Plasmid

#241817

Purpose

Expresses Myc-tagged and LgBiT-tagged Ubiquitin for use with the Promega HiBiT system

Depositor

Ethan Lee

Article

Kassel et al Nat Commun. 2025 Jun 5;16(1):5248. doi:

Insert

Ubiquitin

Tags

LgBiT and Myc

Expression

Mammalian

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pRP-B-catenin-V5-LgBiT

Plasmid

#241816

Purpose

Expresses V5-tagged and LgBiT-tagged B-catenin for use with the Promega HiBiT system

Depositor

Ethan Lee

Article

Kassel et al Nat Commun. 2025 Jun 5;16(1):5248. doi:

Insert

B-catenin

Tags

LgBiT and V5

Expression

Mammalian

Available Since

Aug. 6, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pFRT-TO-FLAG(N)-CDC73

Plasmid

#231751

Purpose

Expression in Flp-IN human cells

Depositor

Jesper Svejstrup

Article

Lopez Martinez et al Mol Cell. 2025 May 15;85(10):1952-1967.e

Insert

CDC73 (CDC73 Human)

Tags

Flag-3C

Expression

Mammalian

Mutation

siCDC73#6 and #8 resistant

Available Since

May 2, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pFRT-TO-FLAG(N)-CDC73deltaKIM

Plasmid

#231752

Purpose

Expression in Flp-IN human cells

Depositor

Jesper Svejstrup

Article

Lopez Martinez et al Mol Cell. 2025 May 15;85(10):1952-1967.e

Insert

CDC73 (CDC73 Human)

Tags

Flag-3C

Expression

Mammalian

Mutation

siCDC73#6 and #8 resistant; CDC73(delta290-324)

Available Since

May 2, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pFRT-TO-FLAG(N)-CDC73-YY/AA

Plasmid

#231753

Purpose

Expression in Flp-IN human cells

Depositor

Jesper Svejstrup

Article

Lopez Martinez et al Mol Cell. 2025 May 15;85(10):1952-1967.e

Insert

CDC73 (CDC73 Human)

Tags

Flag-3C

Expression

Mammalian

Mutation

siCDC73#6 and #8 resistant; CDC73(Y290A, Y293A)

Available Since

May 2, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pLIB-FLAG(N)-CDK12deltaNTD(700-1490)

Plasmid

#231734

Purpose

Protein expression in insect cells

Depositor

Jesper Svejstrup

Article

Lopez Martinez et al Mol Cell. 2025 May 15;85(10):1952-1967.e

Insert

CDK12 (CDK12 Human)

Tags

Flag-3C

Expression

Insect

Mutation

CDK12(700-1490)

Available Since

May 2, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pLIB-FLAG(N)-CDK12-KiD(700-1082)

Plasmid

#231736

Purpose

Protein expression in insect cells

Depositor

Jesper Svejstrup

Article

Lopez Martinez et al Mol Cell. 2025 May 15;85(10):1952-1967.e

Insert

CDK12 (CDK12 Human)

Tags

Flag-3C

Expression

Insect

Mutation

CDK12(700-1082)

Available Since

May 2, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pLIB-FLAG(N)-CDK12deltaRS(delta104-380)

Plasmid

#231737

Purpose

Protein expression in insect cells

Depositor

Jesper Svejstrup

Article

Lopez Martinez et al Mol Cell. 2025 May 15;85(10):1952-1967.e

Insert

CDK12 (CDK12 Human)

Tags

Flag-3C

Expression

Insect

Mutation

CDK12(delta104-380)

Available Since

May 2, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pLIB-FLAG-GST(N)-CDK12RS(104-380)

Plasmid

#231738

Purpose

Protein expression in insect cells

Depositor

Jesper Svejstrup

Article

Lopez Martinez et al Mol Cell. 2025 May 15;85(10):1952-1967.e

Insert

CDK12 (CDK12 Human)

Tags

Flag-GST-3C

Expression

Insect

Mutation

CDK12(104-380)

Available Since

May 2, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pLIB-FLAG(N)-CDC73deltaKIM(delta290-324)

Plasmid

#231739

Purpose

Protein expression in insect cells

Depositor

Jesper Svejstrup

Article

Lopez Martinez et al Mol Cell. 2025 May 15;85(10):1952-1967.e

Insert

CDC73 (CDC73 Human)

Tags

Flag-3C

Expression

Insect

Mutation

CDC73(delta290-324)

Available Since

May 2, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pLIB-FLAG(N)-CDC73-YY/AA(Y290A, Y293A)

Plasmid

#231740

Purpose

Protein expression in insect cells

Depositor

Jesper Svejstrup

Article

Lopez Martinez et al Mol Cell. 2025 May 15;85(10):1952-1967.e

Insert

CDC73 (CDC73 Human)

Tags

Flag-3C

Expression

Insect

Mutation

CDC73(Y290A, Y293A)

Available Since

May 2, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
HR700_HA2TP53_Ex5

Plasmid

#228860

Purpose

Backbone for generation of donor vectors for CRISPR/Cas9 mediated Saturation Genome Editing (SGE) of TP53 Exon 5

Depositor

Thorsten Stiewe

Article

Funk et al Nat Genet. 2025 Jan;57(1):140-153. doi:

Type

Empty backbone

Use

CRISPR and Cre/Lox

Expression

Mammalian

Available Since

Feb. 4, 2025

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
HR700_HA2TP53_Ex6

Plasmid

#228861

Purpose

Backbone for generation of donor vectors for CRISPR/Cas9 mediated Saturation Genome Editing (SGE) of TP53 Exon 6

Depositor

Thorsten Stiewe

Article

Funk et al Nat Genet. 2025 Jan;57(1):140-153. doi:

Type

Empty backbone

Use

CRISPR and Cre/Lox

Expression

Mammalian

Available Since

Feb. 4, 2025

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
HR700_HA2TP53_Ex7

Plasmid

#228862

Purpose

Backbone for generation of donor vectors for CRISPR/Cas9 mediated Saturation Genome Editing (SGE) of TP53 Exon 7

Depositor

Thorsten Stiewe

Article

Funk et al Nat Genet. 2025 Jan;57(1):140-153. doi:

Type

Empty backbone

Use

CRISPR and Cre/Lox

Expression

Mammalian

Available Since

Feb. 4, 2025

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
HR700_HA2TP53_Ex8

Plasmid

#228863

Purpose

Backbone for generation of donor vectors for CRISPR/Cas9 mediated Saturation Genome Editing (SGE) of TP53 Exon 8

Depositor

Thorsten Stiewe

Article

Funk et al Nat Genet. 2025 Jan;57(1):140-153. doi:

Type

Empty backbone

Use

CRISPR and Cre/Lox

Expression

Mammalian

Available Since

Feb. 4, 2025

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
lenti_U6_miR-Oct4e_TRE12_OCT4(co)-mNeonGreen_WPRE

Plasmid

#229544

Purpose

Lentiviral construct to express synthetic miRNA and OCT4-mNeonGreen fusion protein

Depositor

Domitilla Del Vecchio

Article

Ilia et al Sci Adv. 2023 Dec;9(48):eadg8495. doi: 1

Insert

Oct4(co)-mNeonGreen (POU5F1 Human)

Use

Lentiviral

Tags

Oct4-mNeonGreen

Expression

Mammalian

Mutation

codon-optimized OCT4

Promoter

TRE12

Available Since

Jan. 6, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge

Showing: 5881 - 5920 of 7580 results

Results per page:

Sort results by: