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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 16,140 results for: IGH@

Showing: 2941 - 2960 of 16140 results

  1. EBA175-bio

    Plasmid
    #47743
    Purpose
    Expresses enzymatically monobiotinylated full-length EBA175 ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.
    Depositor
    Gavin Wright
    Article
    Crosnier et al Mol Cell Proteomics. 2013 Sep 16.
    Insert
    Codon-optimised EBA175
    Use
    Tags
    enzymatic biotinylation sequence and rat Cd4 d3+4
    Expression
    Mammalian
    Mutation
    Exogenous signal peptide of mouse origin, changed…
    Promoter
    CMV
    Available since
    Feb. 4, 2014
    Availability
    Academic Institutions and Nonprofits only
    47743-plasmid-map-sequence-id-68751

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  3. natMX-ins7

    Plasmid
    #195044
    Purpose
    pFA6a derived selection cassette 5' flanked with tCYC1 transcription terminator, allows genome modification without disruption of insertion neighboring genes by transcription interference
    Depositor
    Gloria Brar
    Article
    Powers et al Elife. 2022 Dec 12;11:e81086. doi: 10.75
    Insert
    NatR
    Use
    Yeast genomic targeting
    Tags
    tCYC1 S. cerevisiae, pPGK1 C. glabrata, NatR, tPG…
    Expression
    Mutation
    Promoter
    Available since
    Feb. 9, 2023
    Availability
    Academic Institutions and Nonprofits only
    195044-plasmid-map-sequence-id-384248

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  5. TTMP-His-bio

    Plasmid
    #52335
    Purpose
    Expresses full-length TPA-induced transmembrane protein ectodomain in mammalian cells. N-terminal His tag, biotinylation sequence and rat Cd4d3+4 tag.
    Depositor
    Gavin Wright
    Article
    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m
    Insert
    TTMP (C3orf52 Human)
    Use
    Tags
    His tag, enzymatic biotinylation sequence, and ra…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Feb. 27, 2015
    Availability
    Academic Institutions and Nonprofits only
    52335-plasmid-map-sequence-id-81056

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  10. INSR-bio-His

    Plasmid
    #53341
    Purpose
    Expresses full-length Insulin receptor precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.
    Depositor
    Gavin Wright
    Article
    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m
    Insert
    INSR (INSR Human)
    Use
    Tags
    His tag, enzymatic biotinylation sequence, and ra…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Feb. 27, 2015
    Availability
    Academic Institutions and Nonprofits only
    53341-plasmid-map-sequence-id-83071

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  11. FCGR2a-bio-His

    Plasmid
    #51754
    Purpose
    Expresses full-length Low affinity immunoglobulin gamma Fc region receptor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.
    Depositor
    Gavin Wright
    Article
    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m
    Insert
    FCGR2a (FCGR2A Human)
    Use
    Tags
    His tag, enzymatic biotinylation sequence, and ra…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Feb. 27, 2015
    Availability
    Academic Institutions and Nonprofits only
    51754-plasmid-map-sequence-id-79338

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  14. pX330-Flag-SpCas9-HF1 (without sgRNA)

    Plasmid
    #92102
    Purpose
    Expression plasmid for human codon-optimized high-fidelity SpCas9-HF1, px330-like backbone (without U6-sgRNA coding sequence)
    Depositor
    Ervin Welker
    Article
    Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:
    Insert
    3xFlag-NLS-Streptococcus pyogenes Cas9-HF1-NLS
    Use
    CRISPR
    Tags
    3xFLAG and NLS
    Expression
    Mammalian
    Mutation
    N497A, R661A, Q695A, Q926A
    Promoter
    Cbh
    Available since
    Oct. 31, 2017
    Availability
    Academic Institutions and Nonprofits only
    92102-plasmid-map-sequence-id-197631

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  15. pPHR-mCherry-NLS-VP16

    Plasmid
    #103821
    Purpose
    Soluble BLInCR transcription activator (transactivation domain of viral VP16 protein) that is recruited to 'localizer' sites upon blue light illumination
    Depositor
    Karsten Rippe
    Article
    Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422
    Insert
    PHR-mCherry-VP16 (CRY2 Mustard Weed)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    VP16: G126T (silent, G42G)
    Promoter
    CMV
    Available since
    May 25, 2018
    Availability
    Academic Institutions and Nonprofits only
    103821-plasmid-map-sequence-id-203431

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  16. RH2b-bio

    Plasmid
    #47778
    Purpose
    Expresses enzymatically monobiotinylated partial RH2b ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.
    Depositor
    Gavin Wright
    Article
    Crosnier et al Mol Cell Proteomics. 2013 Sep 16.
    Insert
    Codon-optimised RH2b (partial)
    Use
    Tags
    enzymatic biotinylation sequence and rat Cd4 d3+4
    Expression
    Mammalian
    Mutation
    Exogenous signal peptide of mouse origin, changed…
    Promoter
    CMV
    Available since
    Feb. 4, 2014
    Availability
    Academic Institutions and Nonprofits only
    47778-plasmid-map-sequence-id-68797

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  18. pPHR-iRFP713-NLS-VP16

    Plasmid
    #103823
    Purpose
    Soluble BLInCR transcription activator (transactivation domain of viral VP16 protein) that is recruited to 'localizer' sites upon blue light illumination
    Depositor
    Karsten Rippe
    Article
    Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422
    Insert
    PHR-iRFP713-VP16 (CRY2 Mustard Weed)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    VP16: G126T (silent, G42G)
    Promoter
    CMV
    Available since
    Dec. 1, 2017
    Availability
    Academic Institutions and Nonprofits only
    103823-plasmid-map-sequence-id-199372

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  19. RBP2-like-bio

    Plasmid
    #68536
    Purpose
    Expresses full-length P. vivax RBP2-like (PVX_101590) ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence
    Depositor
    Gavin Wright, Julian Rayner
    Article
    Hostetler et al PLoS Negl Trop Dis. 2015 Dec 23;9(12):e0
    Insert
    RBP2-like
    Use
    Tags
    rat CD4 d3+4, enzymatic biotinylation sequence
    Expression
    Mammalian
    Mutation
    All NXT/S sites mutated to NXA, where X is any am…
    Promoter
    CMV
    Available since
    Sept. 17, 2015
    Availability
    Academic Institutions and Nonprofits only
    68536-plasmid-map-sequence-id-120662

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  20. APCS-bio-His

    Plasmid
    #53424
    Purpose
    Expresses full-length Serum amyloid P-component precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.
    Depositor
    Gavin Wright
    Article
    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m
    Insert
    APCS (APCS Human)
    Use
    Tags
    His tag, enzymatic biotinylation sequence, and ra…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Feb. 27, 2015
    Availability
    Academic Institutions and Nonprofits only
    53424-plasmid-map-sequence-id-83212

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Showing: 2941 - 2960 of 16140 results