We narrowed to 18,759 results for: nls

Showing: 5921 - 5960 of 18759 results

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TOG_D1

Plasmid

#139112

Purpose

express first domain (1-250) of TOG

Depositor

Nicolas Fawzi

Publication

Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

Insert

TOG/CKAP5 (CKAP5 Human)

Expression

Bacterial

Mutation

1-250

Available since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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hnRNPF_PLD

Plasmid

#139113

Purpose

express prion like domain of hnRNPF

Depositor

Nicolas Fawzi

Publication

Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

Insert

hnRNPF (HNRNPF Human)

Expression

Bacterial

Mutation

365-415

Available since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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hnRNPA2_LC_NtoS

Plasmid

#139121

Purpose

express hnRNPA2 with all N changed to S

Depositor

Nicolas Fawzi

Publication

Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

Insert

hnRNPA2 (HNRNPA2B1 Human)

Expression

Bacterial

Mutation

all N to S

Available since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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hnRNPA2_LC_5E

Plasmid

#139122

Purpose

express hnRNPA2 with 5 serine changed to glutamate

Depositor

Nicolas Fawzi

Publication

Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

Insert

hnRNPA2 (HNRNPA2B1 Human)

Expression

Bacterial

Mutation

5 serine to glutamate

Available since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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hnRNPA2_LC_12E

Plasmid

#139123

Purpose

express hnRNPA2 with all serine change to glutamate

Depositor

Nicolas Fawzi

Publication

Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

Insert

hnRNPA2 (HNRNPA2B1 Human)

Expression

Bacterial

Mutation

12 S to E

Available since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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hnRNPF_PLD_StoA

Plasmid

#139125

Purpose

express hnRNPF PLD with all S changed to A

Depositor

Nicolas Fawzi

Publication

Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

Insert

hnRNPA2 (HNRNPA2B1 Human)

Expression

Bacterial

Mutation

all S to A

Available since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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kank1b_pJet1.2

Plasmid

#121979

Purpose

for generating kank1b riboprobes for WISH in zebrafish

Depositor

GuangJun Zhang

Publication

Hensley et al Sci Rep. 2016 Jun 13;6:27816. doi: 10.10

Insert

kank1b (si:dkey-226m8.9 Zebrafish)

Use

Cloning vector

Promoter

T7

Available since

Feb. 28, 2019

Availability

Academic Institutions and Nonprofits only

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kank1a_pJet1.2

Plasmid

#121978

Purpose

for generating kank1a riboprobes for WISH in zebrafish

Depositor

GuangJun Zhang

Publication

Hensley et al Sci Rep. 2016 Jun 13;6:27816. doi: 10.10

Insert

kank1a (si:dkey-146l2.1 Zebrafish)

Use

Cloning vector

Promoter

T7

Available since

Feb. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pET29b-clbH-C-A2-PCP S1524A

Plasmid

#114158

Purpose

Expresses ClbH-C-A2-PCP S1524A

Depositor

Emily Balskus

Publication

Zha et al Nat Chem Biol. 2017 Oct;13(10):1063-1065

Insert

clbH

Tags

S-Tag, Thrombin site

Expression

Bacterial

Mutation

Only contains the C-A2-PCP domains of ClbH with S…

Available since

Sept. 5, 2018

Availability

Academic Institutions and Nonprofits only

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pET28a-clbH-C-A2-PCP

Plasmid

#114157

Purpose

Expresses ClbH-C-A2-PCP

Depositor

Emily Balskus

Publication

Zha et al Nat Chem Biol. 2017 Oct;13(10):1063-1065

Insert

clbH

Tags

6xHis-thrombin-T7 tag

Expression

Bacterial

Mutation

Only contains the C-A2-PCP domains of ClbH

Available since

Aug. 31, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3-myc NES-mCE

Plasmid

#82463

Purpose

Expression of myc epitope and murine mRNA Capping Enzyme with Terminal nuclear export signal in mammalian cells

Depositor

Daniel Schoenberg

Publication

Otsuka et al Mol Cell Biol. 2009 Apr;29(8):2155-67. d

Insert

NES mRNA capping enzyme (Rngtt Mouse, HIV)

Tags

myc

Expression

Mammalian

Promoter

CMV

Available since

Sept. 9, 2016

Availability

Academic Institutions and Nonprofits only

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SepOTSι

Plasmid

#68290

Purpose

Phosphoserine orthogonal translation system

Depositor

Jesse Rinehart

Publication

Pirman et al Nat Commun. 2015 Sep 9;6:8130. doi: 10.1

Insert

SepRS9/EFSep21, 2xtRNA-Sep-A37

Use

Synthetic Biology

Expression

Bacterial

Available since

Sept. 11, 2015

Availability

Academic Institutions and Nonprofits only

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SepOTSη

Plasmid

#68288

Purpose

Phosphoserine orthogonal translation system

Depositor

Jesse Rinehart

Publication

Pirman et al Nat Commun. 2015 Sep 9;6:8130. doi: 10.1

Insert

SepRS9/EF-Sep21, 2xtRNA-Sep

Use

Synthetic Biology

Expression

Bacterial

Available since

Sept. 11, 2015

Availability

Academic Institutions and Nonprofits only

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SepOTSζ

Plasmid

#68287

Purpose

Phosphoserine orthogonal translation system

Depositor

Jesse Rinehart

Publication

Pirman et al Nat Commun. 2015 Sep 9;6:8130. doi: 10.1

Insert

SepRS/EF-Sep, 2xtRNA-Sep

Use

Synthetic Biology

Expression

Bacterial

Available since

Sept. 11, 2015

Availability

Academic Institutions and Nonprofits only

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SepOTSδ

Plasmid

#68285

Purpose

Phosphoserine orthogonal translation system

Depositor

Jesse Rinehart

Publication

Pirman et al Nat Commun. 2015 Sep 9;6:8130. doi: 10.1

Insert

SepRS/EF-Sep, 1xtRNA-Sep-A37

Use

Synthetic Biology

Expression

Bacterial

Available since

Sept. 10, 2015

Availability

Academic Institutions and Nonprofits only

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Zed_vector_tol2_-2.1prox1a_1:EGFP_XCA:DsRed2

Plasmid

#218205

Purpose

Zebrafish -2.1prox1a enhancer reporter in the lymphatic valve from 3 dpf. XCA:DsRed2 as a control in the skeletal and cardiac muscle. Shows the high background typical of the ZED vector

Depositor

Katarzyna Koltowska

Publication

Panara et al Development. 2024 May 1;151(9):dev202525

Insert

-2.1prox1a

Use

Zebrafish expression

Available since

Aug. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pInt-ERCreER

Plasmid

#190040

Purpose

This is an integration donor plasmid to insert a DNA fragment for doxycycline inducible ERT2-Cre-ERT2 at the AAVS1 locus. It works with an AAVS1 targeting CRISPR/Cas9 construct (Addgene #72833).

Depositor

Kayoko Tanaka

Publication

Hindul et al Biol Open. 2022 Feb 15;11(2). pii: 27408

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

ERT2-Cre-ERT2 flanked by AAVS1

Use

CRISPR and TALEN

Expression

Mammalian

Available since

Jan. 16, 2024

Availability

Academic Institutions and Nonprofits only

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TOPO-HIF1A

Plasmid

#226452

Purpose

For subcloning of human HIF1A promoter or for assays using M13 phage

Depositor

Glen Borchert

Publication

DeMeis et al Nucleic Acids Res. 2025 Jan 11;53(2):gka

Insert

HIF1A (HIF1A Human)

Use

Cloning vector

Mutation

No

Available since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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TDP-43_NTD1-80_Y4R

Plasmid

#104478

Purpose

bacterial expression of TDP-43 NTD Y4R

Depositor

Nicolas Fawzi

Publication

Wang et al EMBO J. 2018 Mar 1;37(5). pii: embj.2017

Insert

TDP-43 (TARDBP Human)

Tags

His

Expression

Bacterial

Mutation

Y4R

Available since

March 20, 2018

Availability

Academic Institutions and Nonprofits only

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FUS LC-RGG1 wild type

Plasmid

#226596

Purpose

Wild type sequence of FUS LC-RGG1 domains

Depositor

Nicolas Fawzi

Publication

Wake et al Nat Chem Biol. 2025 Jul;21(7):1076-1088.

Insert

FUS LC-RGG1 WT (FUS Human)

Tags

6His

Expression

Bacterial

Mutation

wild type LC-RGG1

Promoter

T7

Available since

July 1, 2025

Availability

Academic Institutions and Nonprofits only

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FUS LC 4S->A#1

Plasmid

#226603

Purpose

4 serines to alanine at residue positions 30, 44, 48, 53

Depositor

Nicolas Fawzi

Publication

Wake et al Nat Chem Biol. 2025 Jul;21(7):1076-1088.

Insert

FUS LC S->A#1 (FUS Human)

Tags

6His

Expression

Bacterial

Mutation

4 serines to alanine in sequence position subset …

Promoter

T7

Available since

July 1, 2025

Availability

Academic Institutions and Nonprofits only

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FUS LC 4S->A#2

Plasmid

#226604

Purpose

4 serines to alanine at residue positions 70, 84, 89, 95

Depositor

Nicolas Fawzi

Publication

Wake et al Nat Chem Biol. 2025 Jul;21(7):1076-1088.

Insert

FUS LC 4S->A#2 (FUS Human)

Tags

6His

Expression

Bacterial

Mutation

4 serines to alanine in sequence position subset …

Promoter

T7

Available since

July 1, 2025

Availability

Academic Institutions and Nonprofits only

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FUS LC 4S->A#3

Plasmid

#226605

Purpose

4 serines to alanine at residue positions 108, 117, 119, 127

Depositor

Nicolas Fawzi

Publication

Wake et al Nat Chem Biol. 2025 Jul;21(7):1076-1088.

Insert

FUS LC 4S->A#3 (FUS Human)

Tags

6His

Expression

Bacterial

Mutation

4 serines to alanine in sequence position subset …

Promoter

T7

Available since

July 1, 2025

Availability

Academic Institutions and Nonprofits only

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pKrev-CGI

Plasmid

#225568

Purpose

packaging plasmid for lentivector production

Depositor

Ferdinando Pucci

Publication

Ziglari et al Cancer Res. 2025 Mar 3;85(5):859-874. do

Type

Empty backbone

Expression

Mammalian

Available since

March 12, 2025

Availability

Academic Institutions and Nonprofits only

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p48.unipolyA.dNGFR.mCMV.PGK.eGFP.Gem1.WPRE

Plasmid

#226767

Purpose

vector with NGFR and GFP

Depositor

Ferdinando Pucci

Publication

Ziglari et al Cancer Res. 2025 Mar 3;85(5):859-874. do

Type

Empty backbone

Expression

Mammalian

Available since

March 12, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
p49.unipolyA.Cdt1.mSca.mCMV.PGK.eGFP.Gem1.WPRE

Plasmid

#226768

Purpose

vector with mScarlet fluorescent protein and GFP

Depositor

Ferdinando Pucci

Publication

Ziglari et al Cancer Res. 2025 Mar 3;85(5):859-874. do

Type

Empty backbone

Expression

Mammalian

Available since

March 12, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pDIV495

Plasmid

#177702

Purpose

Plasmid expressing optimized Cas9 and NAT marker and sgRNA targeting ADE2 locus in D. hansenii

Depositor

Uffe Mortensen

Publication

Strucko et al Synth Biol (Oxf). 2021 Oct 12;6(1):ysab0

Inserts

Cas9
Nat
sgRNA Targeting ADE2 locus in D.hansenii: AGCTAaGCAGATTAAtGCAT

Use

CRISPR

Tags

SV40

Expression

Bacterial and Yeast

Promoter

RNR2p (Debaryomyces hansenii ), SRC1p (Debaryomyc…

Available since

March 11, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pHA841 - hrp-1p::hrp-1HsLCWT

Plasmid

#139198

Purpose

Expresses hrp-1p::hrp-1HsLCWT::hrp-1 3'UTR

Depositor

Anne Hart

Publication

Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

Insert

hrp-1 (hrp-1 Nematode)

Expression

Worm

Mutation

last exon replaced with correspoding human sequen…

Available since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pHA842 - hrp-1p::hrp-1HsLCD290V

Plasmid

#139199

Purpose

Expresses hrp-1p::hrp-1HsLCD290V::hrp-1 3'UTR

Depositor

Anne Hart

Publication

Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

Insert

hrp-1 (hrp-1 Nematode)

Expression

Worm

Mutation

D290V, last exon replaced with correspoding human…

Available since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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FUS_LC_withR

Plasmid

#139128

Purpose

express FUS LC with the same number and spacing of arginines as hnRNPA2 LC

Depositor

Nicolas Fawzi

Publication

Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

Insert

FUS (FUS Human)

Expression

Bacterial

Mutation

add arginines

Available since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pJ4M_HRP-1_FL

Plasmid

#139117

Purpose

express HRP-1 with a C-terminal maltose binding protein tag

Depositor

Nicolas Fawzi

Publication

Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

Insert

hrp-1 (hrp-1 Nematode)

Expression

Bacterial

Mutation

None

Available since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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hnRNPA2_LC_nocharge_wR

Plasmid

#139119

Purpose

express hnRNPA2 LC with no charged residues with R added back

Depositor

Nicolas Fawzi

Publication

Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

Insert

hnRNPA2 (HNRNPA2B1 Human)

Expression

Bacterial

Mutation

remove all K, most D

Available since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
hnRNPA2_LC_RtoK

Plasmid

#139120

Purpose

express hnRNPA2 LC where all R are K

Depositor

Nicolas Fawzi

Publication

Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

Insert

hnRNPA2 (HNRNPA2B1 Human)

Expression

Bacterial

Mutation

all R to K

Available since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
hnRNPF_PLD_YtoS

Plasmid

#139124

Purpose

express hnRNPF PLD with all Y changed to S

Depositor

Nicolas Fawzi

Publication

Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

Insert

hnRNPA2 (HNRNPA2B1 Human)

Expression

Bacterial

Mutation

all Y to S

Available since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
FUS_LC_charge

Plasmid

#139126

Purpose

express FUS LC with an hnRNPA2 LC-like charge patterning

Depositor

Nicolas Fawzi

Publication

Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

Insert

FUS (FUS Human)

Expression

Bacterial

Mutation

Add R, K, D to make FUS LC have similar charge pa…

Available since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pHEN-CoV2-nanobody-F1

Plasmid

#194592

Purpose

Bacterial expression of F1 nanobody targeting the receptor binding domain (RBD) of SARS-CoV-2 spike

Depositor

Gerald McInerney

Publication

Hanke et al Sci Adv. 2022 Mar 25;8(12):eabm0220. doi

Insert

F1 (S )

Expression

Bacterial

Available since

Feb. 1, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-hSyn-DIO-FLAG-KORD-P2A-3xHA-hM3D(Gq)

Plasmid

#220611

Purpose

Neuron-specific, Cre-dependent co-expression of FLAG-tagged inhibitory KORD DREADD receptor, and 3x HA-tagged excitatory hM3D(Gq) DREADD receptor.

Depositor

Sam Golden

Publication

Szelenyi et al Proc Natl Acad Sci U S A. 2024 Aug 6;121

Insert

FLAG-KORD-P2A-3x HA-hM3D(Gq)

Use

AAV and Cre/Lox

Tags

FLAG; ArgiNLS

Expression

Mammalian

Promoter

hSyn

Available since

Aug. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pYTRW22K_7Ti1

Plasmid

#177293

Purpose

genomic integration of genes (inserted in I-SceI-site) via rrn-interposon with TcR and LacZ

Depositor

Karl-Erich Jaeger

Publication

Weihmann et al FEMS Microbes. 2022 Dec 19;4:xtac030. do

Insert

lacZ

Use

Synthetic Biology; Yeast expression, rrn genomic …

Expression

Bacterial

Available since

Aug. 6, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pYTRW09K_0T5

Plasmid

#177281

Purpose

genomic integration of genes (inserted in I-SceI-site) via transposon Tn5 with TcR

Depositor

Karl-Erich Jaeger

Publication

Weihmann et al FEMS Microbes. 2022 Dec 19;4:xtac030. do

Insert

Ptet-tetA(C)

Use

Synthetic Biology; Yeast expression, tn5 genomic …

Expression

Bacterial

Available since

Aug. 6, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pNMTMA_MBP-Cav1-His6

Plasmid

#223700

Purpose

To produce standard caveospheres (without the Z-domain), Parton Lab clone LDB

Depositor

Rob Parton

Publication

Luong et al ACS Nano. 2025 Dec 23;19(50):42079-42096

Insert

MBP-Cav1-His6

Expression

Bacterial

Available since

Oct. 3, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge

Showing: 5921 - 5960 of 18759 results

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We narrowed to 18,759 results for: nls