We narrowed to 9,352 results for: UTY
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Plasmid#70185PurposeConstiutive mammalian expression of the bioluminescent GpNLuc LumiFluor reporter for single-cell analysis and sensitive optical imaging of living subjects..DepositorTypeEmpty backboneUseRetroviralTagsExpressionMammalianMutationPromoterPGKAvailable sinceDec. 2, 2015AvailabilityAcademic Institutions and Nonprofits only
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pKD239-1g-2
Plasmid#90959PurposeExpresses S. baltica TtrR and mCherry constitutively and sfGFP under the PttrB185-269 promoterDepositorInsertsTetrathionate response regulator
Superfolder GFP
mCherry
UseTagsExpressionBacterialMutationPromoterBba_J23100, Bba_J23109, and PttrB185-269Available sinceMay 16, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCD5-D/bovine/Nebraska/9-5/2012-HEFwtED-GCN4-sfGFP-ST
Plasmid#175019PurposeExpresses influenza D virus trimeric hemagglutinin esterase fusion protein fused to a sfGFPDepositorInsertD/bovine/Nebraska/9-5/2012
UseTagsGCN4-TEV-sfGFP-TwinStrepExpressionMammalianMutationPromoterCMVAvailable sinceJan. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
FUW-H2B-mTurquoise
Plasmid#193093Purposeconstitutive expression of H2B-mTurquoise in mammalian cellsDepositorInsertH2B-mTurquoise
UseLentiviralTagsExpressionMammalianMutationPromoterUbcAvailable sinceAug. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRetroX-Tight-MCS_PGK-OgNLuc
Plasmid#70186PurposeConstiutive mammalian expression of the bioluminescent OgNLuc LumiFluor reporter for single-cell analysis and sensitive optical imaging of living subjects.DepositorTypeEmpty backboneUseRetroviralTagsExpressionMammalianMutationPromoterPGKAvailable sinceDec. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pX330-Cetn1/1
Plasmid#50718PurposepX330 containing sgRNA against mouse Cent1. Positive control for DSB mediated EGFP reconstitution.DepositorInsertsCetn1 sgRNA1
humanized S. pyogenes Cas9
UseCRISPRTags3xFLAGExpressionMammalianMutationPromoterCBh and hU6Available sinceFeb. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-ALK7 T194D
Plasmid#80886Purposemammalian expression of ALK7 T194DDepositorInsertALK7 (Acvr1c Rat)
UseTagsHAExpressionMammalianMutationT194D (constitutively active)PromoterCMVAvailable sinceAug. 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHR_PGK_SNIPR_(GGS)6_SNIPR
Plasmid#188344PurposeLentiviral vector - constitutive expression of an anti-CD19 SNIPR with a (GGS)6, a Notch1-TMD, a Notch1-JMD, and a Gal4VP64 transcriptional factorDepositorInsertPGK_antiCD19_(GGS)6_Notch1-TMD_Notch1-JMD_Gal4VP64
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMarch 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHR_PGK_SNIPR_FnIII-Notch
Plasmid#188342PurposeLentiviral vector - constitutive expression of an anti-CD19 SNIPR with a FnIII-ECD, a Notch1-TMD, a Notch1-JMD, and a Gal4VP64 transcriptional factorDepositorInsertPGK_antiCD19_FnIII-ECD_Notch1-TMD_Notch1-JMD_Gal4VP64
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMarch 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
LbCas12a-2C-NLS in pCSDest
Plasmid#126638PurposeExpresses LbCas12a-2C-NLS in mammalian cellDepositorInsertLbCas12a-2C-NLS
UseCRISPRTags3xHuman influenza hemagglutinin (HA)-TagExpressionMammalianMutationPromoterCMV IE94 promoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMRE147
Plasmid#118499Purposebroad-host range plasmid to constitutively express fluorescent protein genes in bacteriaDepositorInsertmClover3
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-hfYFP
Plasmid#191201PurposeConstitutive CAG-driven expression of hfYFP in animals using AAVDepositorInsertHyperfolder YFP
UseAAVTagsExpressionMammalianMutationmGreenLantern-F46L/C48S/V68L/A69M/C70V/K101E/T105ā¦PromoterCAGAvailable sinceDec. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMRE143
Plasmid#118495Purposebroad-host range plasmid to constitutively express fluorescent protein genes in bacteriaDepositorInsertsYFP2
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
PlepA
Plasmid#55175PurposeStrong constitutive promoterDepositorInsertlepA promoter
UseSynthetic Biology; Bacillus biobrick boxTagsExpressionMutationPromoterAvailable sinceJuly 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLX302 OAS2-V5 puro
Plasmid#158642PurposeLentiviral expression vector for constitutive expression of V5-tagged OAS2.DepositorInsertOAS2 (OAS2 Human)
UseLentiviralTagsV5ExpressionMutationPromoterAvailable sinceOct. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMRE165
Plasmid#118513Purposebroad-host range plasmid to constitutively express fluorescent protein genes in bacteriaDepositorInsertmScarlet-I
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CMV-H2B-3xFLAG-2A-G(N2c)
Plasmid#194355PurposeAAV constitutively expressing nuclear FLAG and Rabies Glycoprotein (G) of CVS-N2c strainDepositorInsertsH2B-3xFLAG
Rabies CVS-N2c glycoprotein (G)
UseAAVTagsFLAGExpressionMutationPromoterAvailable sinceAug. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCRISPRi
Plasmid#199808Purposevector for constitutive CRISPRi-mediated gene knockdown in S. aureusDepositorInsertsdCas9
sgRNA with GFP cassette
UseCRISPRTagsExpressionMutationPromoterP23 and PRAB17 (sgRNA) and SarAP1 (GFP)Available sinceJuly 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKD184
Plasmid#90953PurposeExpresses ThsR under the PltetO-1 promoter, sfGFP under PphsA, and mCherry constitutivelyDepositorInsertsThiosulfate response regulator
Superfolder GFP
mCherry
UseTagsExpressionBacterialMutationPromoterBba_J23114, PltetO-1, and PphsAAvailable sinceMay 16, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCG-TPMV Hd32-IL-13
Plasmid#163869PurposeExpresses TPMV H glycoprotein bearing IL-13 in mammalian cellsDepositorInsertTPMV H glycoprotein/IL-13
UseTagsHuman IL-13ExpressionMammalianMutationAmino acids 2 to 33 at N terminus deletedPromoterAvailable sinceMay 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
Burden Monitor phi80 version
Plasmid#66074PurposeThe burden monitor was cloned inside plasmid pAH153 [Haldimann2001]; the genome integration of the system allows the measurement of the burden imposed by synthetic constructs to Escherichia coli cellDepositorInsertGFP
UseTagsExpressionMutationPromoterAvailable sinceJuly 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pet-15b-microID
Plasmid#172881Purposeexpresses microID in E. coliDepositorInsertmicroID
UseTagsmycExpressionBacterialMutationfragment ([aa2-171]) of aquifex aeolicus BirA witā¦PromoterT7Available sinceAug. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAS1yl
Plasmid#73226PurposeConstitutive expression of Cas9 and sgRNA in Yarrowia lipolytica cellsDepositorInsertsCas9
sgRNA
UseCRISPR; In y. lipolyticaTagsExpressionBacterialMutationPromoterunknownAvailable sinceJune 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMRE161
Plasmid#118509Purposebroad-host range plasmid to constitutively express fluorescent protein genes in bacteriaDepositorInsertmTurquoise2
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
Bxb1-GA_CAG-ITPKA-3x-mScarlet-I
Plasmid#229782PurposeBxb1-GA donor plasmid for integrating a transcription unit for constitutive expression of a mScarlet-I F-actin reporter (3 copies)DepositorInsert3x mScarlet-I
UseSynthetic BiologyTagsITPKAExpressionMammalianMutationPromoterCAGAvailable sinceFeb. 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHES830
Plasmid#87944PurposeConstitutively expresses progesterone-inducible synthetic transcription factor in yeast. It activates transcription of promoters containing Zif268 binding sites.DepositorInsertZif268 DBD - hPR LBD - MSN2 AD (PGR Synthetic, Budding Yeast, Human)
UseTagsExpressionYeastMutationPromoterADH1 (crippled)Available sinceJan. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMRE164
Plasmid#118512Purposebroad-host range plasmid to constitutively express fluorescent protein genes in bacteriaDepositorInsertmOrange2
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLenti-FNLSHiFiNG-P2A-Puro
Plasmid#136903PurposeLentivirus for constitutive expression of FNLSHiFiNG in mammalian cells (codon optimized)DepositorInsertFNLSHiFiNG
UseLentiviralTagsFLAG-NLS and NLSExpressionMutationR691A,L1111R,D1135V,G1218R,E1219F,A1322R,R1335V,Tā¦PromoterEF1sAvailable sinceFeb. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pKD233.7-3
Plasmid#90955PurposeExpresses S. baltica TtrR under the PltetO-1 promoter, sfGFP under PttrB185-269, and mCherry constitutivelyDepositorInsertsTetrathionate response regulator
Superfolder GFP
mCherry
UseTagsExpressionBacterialMutationPromoterBba_J23114, PltetO-1, and PttrB185-269Available sinceMay 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pHR-LexAOx4-Pmin-Fluc-PGK-Rluc-P2A-mCherry
Plasmid#192634PurposeLentiviral vector for inducible Fluc expression with 4x LexA operator (LexAO) and constitutive Rluc and mCherry.DepositorInsertfirefly luciferase
UseLentiviralTagsExpressionMammalianMutationPromoterMinimal promoterAvailable sinceNov. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV7.1_3xFLAG-WWC3
Plasmid#172996Purposeconstitutive expression of FLAG-tagged WWC3DepositorInsert3xFLAG-WWC3 (WWC3 Human)
UseTags3xFLAGExpressionMammalianMutationPromoterAvailable sinceOct. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFD115
Plasmid#124768PurposepFD115 carries cas9 controlled by an aTc-inducible Ptet promoter, a sgRNA controlled constitutively from PpflB S. aureus promoter to clone a guide between two BsaI sites and oriT on pLZ12 vectorDepositorInsertsPtet
cas9
term PpflB sgRNA (BsaI)
UseCRISPRTagsExpressionMutationPromoterPtetAvailable sinceAug. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHLuc Control
Plasmid#141091PurposepH-stable bioluminescent reporter that serves as the control counterpart of pHLuc; utilizes eGFP instead of SEPDepositorInsertAntares-T2A-puromycin-IRESv24-eGFP-Nanoluc
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
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pACT2-μ3A
Plasmid#198174PurposeExpression of GAL4 transcriptional activation domain (AD)-AP-3 μ3A fusion protein in yeast (yeast two-hybrid assays)DepositorInsertAP-3 μ3A
UseTagsGAL4 transcriptional activation domain (AD) fragmā¦ExpressionYeastMutationsilent substitution in codon 225PromoterADH1Available sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
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pAXEF-Rap1-E63(GTP)
Plasmid#32698DepositorInsertRAP1 (RAP1A Human)
UseTagsExpressionMammalianMutationE63: Consitutively GTP-boundPromoterEF promoterAvailable sinceDec. 5, 2011AvailabilityAcademic Institutions and Nonprofits only -
pUB-spHRed
Plasmid#84734PurposeExpresses pHRed in plant cells-biosensor for pHRed targeted to chloroplasts stomaDepositorInsertpHRed
UseTagsRbcS targeting sequenceExpressionPlantMutationPromoterubiqutin- 10 gene promoter (pUBQ10) of ArabidopsisAvailable sinceJune 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBGSA FPR1-EGFP
Plasmid#62604PurposeConstitutive expression of FPR1-EGFP in mammalian cellsDepositorInsertFPR1 (FPR1 Human)
UseTagsEGFPExpressionMammalianMutationPromoterSRalphaAvailable sinceJune 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDV02_linear_entry_TagBFP-2A-MCS-2A-mNeonGreen
Plasmid#200401PurposeEntry vector for open-loop ADAR-based RNA sensors. Expresses TagBFP constitutively. Expresses mNeonGreen upon target detectionDepositorInsertOpen-loop DART VADAR sensor entry cassette
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceJune 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMRE141
Plasmid#118493Purposebroad-host range plasmid to constitutively express fluorescent protein genes in bacteriaDepositorInsertmTurquoise2
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSW002-PpsbA-DsRed-Express2
Plasmid#111257PurposeBroad host-range bacterial expression vector with constitutive PsbA promoter; for tagging bacteria with DsRed-Express2DepositorInsertDsRed-Express2
UseTagsExpressionBacterialMutationPromoterPpsbAAvailable sinceAug. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
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pGMCtN-TSC10M1-sspB
Plasmid#49521PurposeNourseothricin resistant; expresses sspB under the control of a promoter containing PtetO and constitutively expressses single-chain TetR10 (tsc10); integrates into mycobacteriophage att-Tweety siteDepositorInsertTSC10M1-sspB
UseTagsExpressionMutationPromoterAvailable sinceJan. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pFLAG_attP
Plasmid#110095PurposeVector for low-level constitutive expression in mycobacteria from the Tet promoter. Lacks the Tet repressor. Contains attP for chromosomal integration. Lacks the integrase and thus remains stably integrated in the absence of antibiotic selection. Contains a C-terminal 3xFLAG to allow detection of expression levels by Western blotting.DepositorTypeEmpty backboneUseTags3xFLAGExpressionBacterialMutationPromoterAvailable sinceJune 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBEAST-J23101-sfGFP
Plasmid#116917PurposeStrong constitutive expression of super-folder GFP for cell-free expressionDepositorInsertsfGFP
UseSynthetic Biology; Cell-free protein synthesisTagsExpressionMutationPromoterJ23101Available sinceApril 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSW002-Pc-DsRed-Express2
Plasmid#111251PurposeBroad host-range bacterial expression vector with constitutive Pc promoter; for tagging bacteria with DsRed-Express2DepositorInsertDsRed-Express2
UseTagsExpressionBacterialMutationPromoterPcAvailable sinceAug. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHelper-CBE-sgRNA
Plasmid#221137PurposeHelper plasmid for sgRNA cloning for CRISPR-Cas9 cytosine base editing. sgRNA-scaffold with Cas9 handle expressed from constitutive bacterial promoter J23119(SpeI). 2 BbsI sites for sgRNA cloning.DepositorInsertsgRNA-scaffold with Cas9 handle
UseCRISPRTagsExpressionBacterialMutationPromoterJ23119(SpeI)Available sinceNov. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAVS1_EF1a-MitoTrap-mCherry-IRES-Puro
Plasmid#197067PurposeDonor plasmid for human AAVS1 locus knock-in, constitutive expression of MitoTrap-mCherryDepositorInsertMitoTrap-mCherry
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceSept. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
FUW-cFos
Plasmid#193089Purposeconstitutive expression of mouse cFos in mammalian cellsDepositorInsertcFos
UseLentiviralTagsExpressionMammalianMutationPromoterUbcAvailable sinceAug. 7, 2023AvailabilityAcademic Institutions and Nonprofits only