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Plasmid#177435PurposeTo express mCherry fused to PTDSS1, a protein that localized to mitochondria associated ER membranes (MAMs). Lentiviral vector used to make cell lines expressing this MAM landmark.DepositorInsertPhosphatidylserine synthase-1, PTDSS1, LMHD, PSS1, PSSA (PTDSS1 Human)
UseLentiviralTagsmCherryExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pIVT-myc-T58A-S62D
Plasmid#138540PurposeFor making myc-T58A-S62D RNADepositorInsertMyelocytomatosis oncogene T58A S62D (Myc Mouse)
UseIn vitro transcriptionTagsExpressionMutationThreonine 58 changed to alanine and serine 62 cha…PromoterT7Available sinceJune 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28-his6-HA3-CLIP
Plasmid#188942PurposeT7 expression vector for making his6- and HA3-tagged CLIP proteinDepositorInserthis6-HA3-CLIP
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
HOXA2 (murine) HIS-tag pET
Plasmid#8557DepositorInsertHOXA2 (Hoxa2 Mouse)
UseTagsHis and T7ExpressionBacterialMutationcontains 5 additional amino acids from 5' un…PromoterAvailable sinceMay 30, 2006AvailabilityAcademic Institutions and Nonprofits only -
pUS
Plasmid#153416PurposeBackbone for cloning downstream of UCOE-SFFVDepositorTypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJuly 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
HOXA1 (murine) HIS-tag pET
Plasmid#8556DepositorInsertHOXA1 (Hoxa1 Mouse)
UseTagsHis and T7ExpressionBacterialMutationclone contains 130 AA including the homeodomain, …PromoterAvailable sinceJuly 5, 2006AvailabilityAcademic Institutions and Nonprofits only -
pAptamerCassette-EGFP
Plasmid#140288PurposeBackbone for making protein-responsive mRNA switchesDepositorInsertEmpty backbone
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceOct. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
3xTRE DREaMR 3p3DsR
Plasmid#182206Purpose3xTRE Tet-On cassette with 3xp3 DsRed maker for generating knockins with doxycycline-inducible rescueDepositorInsertrTetR-3XP3-DsRed
UseTagsExpressionInsectMutationPromoterAvailable sinceApril 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMbd3EosHaloTAF_Hyg
Plasmid#140958PurposeTargeting construct to make Mbd3-Eos-Halo-Tev-Avi-3xFlag fusionDepositorInsertmethyl-CpG binding protein 3 (Mbd3 Synthetic, Mouse)
UseMouse TargetingTags3xFLAG, Avi, Eos, Halo, and TEVExpressionMutationPromoterAvailable sinceOct. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTa-HH7-2
Plasmid#124997PurposeTo make transgenic mice for HH7-2 TCRaDepositorInsertHH7-2 TCRa Tg
UseMouse TargetingTagsExpressionMutationPromoterAvailable sinceAug. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTa-HH5-1
Plasmid#124995PurposeTo make transgenic mice for HH5-1 TCRaDepositorInsertHH5-1 TCRa Tg
UseMouse TargetingTagsExpressionMutationPromoterAvailable sinceAug. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET28-ceRab2(1-214)
Plasmid#59550Purposemakes 6x Fusion of Full length ceRab2DepositorInsert1-214
UseTagsExpressionMutationPromoterAvailable sinceOct. 27, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET28-ceRab5(1-208)
Plasmid#59551Purposemakes 6x Fusion of Full length ceRab5DepositorInsert1-208
UseTags6x HisExpressionMutationPromoterAvailable sinceOct. 27, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET28-ceRab10(1-201)
Plasmid#59552Purposemakes 6x Fusion of Full length ceRab10DepositorInsert1-208
UseTags6x HisExpressionMutationPromoterAvailable sinceOct. 27, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET28-ceRab11(1-211)
Plasmid#59553Purposemakes 6x Fusion of Full length ceRab11DepositorInsert1-211
UseTags6x HisExpressionMutationPromoterAvailable sinceOct. 27, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET28-ceRab21(1-207)
Plasmid#59554Purposemakes 6x Fusion of Full length ceRab21DepositorInsert1-207
UseTags6x HisExpressionMutationPromoterAvailable sinceOct. 27, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET28-ceRab21(1-209)
Plasmid#59555Purposemakes 6x Fusion of Full length ceRab35DepositorInsert1-209
UseTags6x HisExpressionMutationPromoterAvailable sinceOct. 27, 2014AvailabilityAcademic Institutions and Nonprofits only -
bactin2:loxP-STOP-loxP-DsRed-express
Plasmid#68797PurposeFor making transgenic fishDepositorInsertsbeta-actin promoter
loxp-stop-codon-loxp-dsred
UseTagsExpressionMutationPromoterAvailable sinceDec. 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
H2B-mGarnet2
Plasmid#104312Purposecodes for a fusion of fluorescent protein mGarnet2 and human histon 2B (H2B), makes the H2B visible in live cellsDepositorInsertH2B-mGArnet2
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceOct. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMSSM110: pCMV-HA-NLS-SpuFz1-v2
Plasmid#205277PurposeHuman expression vector for human codon optimized N-terminal NLS-tagged SpuFz1-v2DepositorInsertSpuFz1
UseTagsHA, NLS(Makkerh)ExpressionMammalianMutationC310R/D487K/T513KPromoterAvailable sinceAug. 8, 2023AvailabilityAcademic Institutions and Nonprofits only