Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

We narrowed to 6,501 results for: nls

Clear keywords
Showing: 2961 - 2980 of 6501 results

  9. deltaVP1 + VP2C-GFPdeg

    Plasmid
    #166677
    Purpose
    Yeast integrative plasmid for expressing Murine polyomavirus deltaVP1 (GAL1 promoter) and destabilised GFP tagged with VP2C (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3).
    Depositor
    Claudia Vickers
    Publication
    Cheah et al ACS Synth Biol. 2021 Dec 17;10(12):3251-
    Inserts
    VP2C-GFPdeg
    Murine polyomavirus deltaVP1 (NLS deletion mutant)
    Use
    Synthetic Biology
    Expression
    Yeast
    Promoter
    GAL1 and GAL10
    Available since
    April 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    166677-plasmid-map-sequence-id-329832

    Enlarge

  10. pcDNA3/ TO myc-cCE-bio (cCE-bio)

    Plasmid
    #82473
    Purpose
    Expresses cytoplasmic restricted form of N-terminal myc tagged and C-terminal biotin tagged mRNA capping enzyme, inactive form
    Depositor
    Daniel Schoenberg
    Publication
    Mukherjee et al PLoS Biol. 2014 Aug 19;12(8):e1001933. d
    Insert
    Myc-NES-mCE (Wt, NLS deletion)-TEV-Bio (Rngtt Mouse, Synthetic)
    Tags
    TEV-Biotin and myc
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Sept. 16, 2016
    Availability
    Academic Institutions and Nonprofits only
    82473-plasmid-map-sequence-id-163076

    Enlarge

  12. pcDNA-LmoCas6-p300

    Plasmid
    #126486
    Purpose
    Encodes L.monocytogenes CRISPR Type I-B Cas6 with N-terminal fusion of Flag epitope and NLS and C-terminal fusion of NLS, human p300 HAT core (aa1048-1664), and Flag tag epitope driven by CMV promoter
    Depositor
    Charles Gersbach
    Publication
    Pickar-Oliver et al Nat Biotechnol. 2019 Sep 23. pii: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    LmoCas6-p300
    Use
    CRISPR
    Tags
    Flag and Flag, NLS
    Expression
    Mammalian
    Promoter
    CMV and U6
    Available since
    Sept. 30, 2019
    Availability
    Academic Institutions and Nonprofits only
    126486-plasmid-map-sequence-id-246633

    Enlarge

  13. pYEE

    Plasmid
    #159750
    Purpose
    Egg cell-specific promoter-controlled expression of 3×FLAG-NLS-zCas9-NLS. Contains gRNA scaffold for insertion of target sequence (U6-26 promoter). Selection of transgenic seeds by YFP fluoresce.
    Depositor
    Wolfgang Lukowitz
    Publication
    Angulo et al bioRxiv
    Insert
    gRNA scaffold
    Use
    CRISPR
    Expression
    Plant
    Available since
    Oct. 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    159750-plasmid-map-sequence-id-313819

    Enlarge

  14. JPF0535a

    Plasmid
    #124042
    Purpose
    Encodes pEF1a driving expression of TET3G P2A iRFP713, pEF1a expressing spdCas9 fused to tagBFP P2A tagBFP, and an sgRNA targeting pTRE expressing mAzamiGreen in a PiggyBac destination vector
    Depositor
    Hana El-Samad
    Publication
    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    PB_pEF1a-tet3G-P2A-iRFP713_pEF1a-spdCAS9::tagBFP-P2A-tagBFP-SV40PA_mu6-sgTRE_pTRE-NLS::mAzamiGreen-rgPA
    Expression
    Mammalian
    Available since
    Dec. 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    124042-plasmid-map-sequence-id-264228

    Enlarge

  15. SFTPC-T2A-Rox-EF1a-Rox-Venus-NLS repair template

    Plasmid
    #167943
    Purpose
    SFTPC reporter targeting repair template
    Depositor
    Emma Rawlins
    Publication
    Sun et al eLife. 2021 Oct 6;10. pii: 67886. doi: 1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SFTPC (SFTPC Human)
    Use
    CRISPR; Donor template
    Tags
    Venus
    Expression
    Mammalian
    Mutation
    homology arms contain point mutations to disrupt …
    Promoter
    EF1a
    Available since
    June 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    167943-plasmid-map-sequence-id-331139

    Enlarge

  16. JG676: CAG-human dLbCpf1(D832A)-NLS-3xHA-3xFLAG-DmrA(X2)

    Plasmid
    #104569
    Purpose
    Mammalian expression vector for catalytically inactive Cpf1 from Lachnospiraceae bacterium (dLbCpf1) fused to two DmrA domains
    Depositor
    Keith Joung
    Publication
    Tak et al Nat Methods. 2017 Oct 30. doi: 10.1038/n
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dLbCpf1(D832A)-DmrA(x2)
    Tags
    3x FLAG, 3x HA, and NLS
    Expression
    Mammalian
    Mutation
    D832A
    Promoter
    CAG
    Available since
    March 29, 2018
    Availability
    Academic Institutions and Nonprofits only
    104569-plasmid-map-sequence-id-201433

    Enlarge

  17. JG674: CAG-human dLbCpf1(D832A)-NLS-3xHA-3xFLAG-DmrA(X1)

    Plasmid
    #104568
    Purpose
    Mammalian expression vector for catalytically inactive Cpf1 from Lachnospiraceae bacterium (dLbCpf1) fused to one DmrA domain
    Depositor
    Keith Joung
    Publication
    Tak et al Nat Methods. 2017 Oct 30. doi: 10.1038/n
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dLbCpf1(D832A)-DmrA(x1)
    Tags
    3x FLAG, 3x HA, and NLS
    Expression
    Mammalian
    Mutation
    D832A
    Promoter
    CAG
    Available since
    March 29, 2018
    Availability
    Academic Institutions and Nonprofits only
    104568-plasmid-map-sequence-id-201431

    Enlarge

  20. PRPF4.001.522.K45A.pc4

    Plasmid
    #137648
    Purpose
    Expresses nuclear localization signal (NLS), C-terminal myc-(His)6 fusion of human gene or portion of gene in human cell culture lines. pcDNA4.1-based vector.
    Depositor
    Tara Davis
    Insert
    PRPF4 (PRPF4 Human)
    Tags
    NLS and myc-(His)6
    Expression
    Mammalian
    Mutation
    001-522-K45A-pc4
    Available since
    Jan. 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    137648-plasmid-map-sequence-id-381373

    Enlarge

Showing: 2961 - 2980 of 6501 results