We narrowed to 6,447 results for: nls
-
Plasmid#145030PurposepiggyBac vector for expressing a Turquoise calcium sensor (GECI) that reports with lifetime and intensity changesDepositorInsert3xnls-Tq-Ca-FLITS
Tags3xnls-Tq-Ca-FLITSExpressionMammalianPromoterCMVAvailable SinceJune 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
Construct 33 - NFKBRp_Cas9_3xNLS_p2a-puroR
Plasmid#81255PurposeLentiviral construct for building stable cell lines expressing Cas9 in the presence of TNFa via puromycin selectionDepositorInsertCas9-3xNLS
UseLentiviral and Synthetic BiologyExpressionMammalianAvailable SinceSept. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
PB-CMV-TO-EGFRvIII-IRES-nlsChe
Plasmid#116039PurposeSupporting plasmid for testing protease circuitsDepositorInsertEGFRvIII-IRES-nlsChe
ExpressionMammalianAvailable SinceOct. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pU6-RNA-pCAG-bpNLS-miCBE-SV40NLS-bGH polyA-pCMV-mCherry-AmpR
Plasmid#248061PurposeExpression vector for expression of miCBE-ωRNA v2 driven by chicken β-actin promoter and mCherry driven by CMV promoter.DepositorInsertpU6-RNA-pCAG-bpNLS-miCBE-SV40NLS-bGH polyA-pCMV-mCherry-AmpR
UseCRISPRTagsflagExpressionMammalianMutationnonePromoterCAG, U6Available SinceJan. 5, 2026AvailabilityAcademic Institutions and Nonprofits only -
NAB2NLS-GFP-PRA (107)
Plasmid#24043DepositorInsertNAB2
TagsEGFP and PrAExpressionYeastMutationOnly bp 601-750 of NAB2 (NLS)Available SinceMay 3, 2010AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-SwitchON_mRubyNLS-hChR2(H134R)-EYFP-NO_WPRE
Plasmid#118279PurposeExpresses mRuby2 in the nucleus of Cre-negative cells, and ChR(H134R)-YFP in Cre-positive cellsDepositorInsertsmRuby2
ChR2(H134R)
UseAAV and Cre/LoxTagsSV40 NLS and YFPPromoterEF1 alphaAvailable SinceDec. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7-BPNLS-CE-ABE8e-SpCas9(D10A)-BPNLS-P2A-EGFP (NK350)
Plasmid#208292PurposeCMV and T7 promoter expression plasmid for human codon optimized CE-ABE8e-SpCas9 A-to-G base editor and P2A-EGFPDepositorInsertpCMV-T7-BPNLS-CE-ABE8e-SpCas9-BPNLS-P2A-EGFP
UseCRISPR; In vitro transcription; t7 promoterTagsBPNLS and BPNLS-P2A-EGFPExpressionMammalianMutationABE8e mutations in TadA inlaid into nSpCas9(D10A)PromoterCMV and T7Available SinceDec. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHS73_10xHis_MBP_TEV_SpRY-Cas9_2xNLS
Plasmid#244835PurposeBacterial expression of SpRY Cas9 with 2xSV40 nuclear localization sequence for protein purificationDepositorInsertSpRY Cas9
UseCRISPRTags10xHis-MBP and 2xSV40 NLSExpressionBacterialMutationA61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1…PromoterT7Available SinceSept. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pT2iC6FAST1nls2ER4(#638)
Plasmid#184068Purposecyclofen-inducible YFAST nuclear relocation via mammalian cell transfection or mRNA synthesisDepositorInsertYFAST-2nls-ERT2
UseSynthetic BiologyExpressionMammalianMutationERT2: G400V, M543A, L544A, deleted 1-281;PromotersCMV+SP6Available SinceJune 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1_N-NLS_POLH_ΔGG NEDD8
Plasmid#221870PurposeExpresses GFP-tagged WT POLH fused to ΔGG NEDD8 in mammalian cellsDepositorAvailable SinceFeb. 23, 2026AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1_N-NLS_POLH_ΔGG ubiquitin
Plasmid#221869PurposeExpresses GFP-tagged WT POLH fused to ΔGG ubiquitin in mammalian cellsDepositorAvailable SinceFeb. 23, 2026AvailabilityAcademic Institutions and Nonprofits only -
pSS22-IRES-NLS-eGFP
Plasmid#241837PurposePart I of the Donor construct for UBE3A reporterDepositorInserteGFP
UseDonor plasmidAvailable SinceSept. 8, 2025AvailabilityAcademic Institutions and Nonprofits only -
CPH3375_SF_Spt6p-tSH2-1223-1451_NLS
Plasmid#227084PurposeGal1p dependent budding yeast expression of tandem-affinity tagged spt6p tSH2 domain (1123-1451) localized to nucleusDepositorInsertspt6-tsh2 (SPT6 Budding Yeast)
TagsSTREPii-FLAG and SV40 NLS 1ExpressionYeastPromoterGal1-10Available SinceNov. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pT7-AGG-NLS-EGFP
Plasmid#214602Purposeexpress EGFP ; in vitro transcribed modified RNA vectorDepositorInsertEGFP
UseUnspecifiedPromoterT7Available SinceOct. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHR-AL-mCh-Cry2WT-NLS
Plasmid#221924PurposeExpression of CBP autoregulatory loop (AL) fragment fused to optogenetic protein mCh-Cry2WTDepositorInsertCBP AL (aa 1529-1607) (CREBBP Human)
UseLentiviralTagsmCherryExpressionMammalianMutationinsertion of the autoregulatory loop (AL) (aa 152…Available SinceJuly 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
eA3Ai-Cas9n-UGI-NLS
Plasmid#207165PurposeExpress A3A with an N57G point mutation and an intron in a BE3-based base editorDepositorInsertApolipoprotein B mRNA Editing Enzyme, Catalytic Polypeptide-like 3A (APOBEC3A Human)
TagsCas9-UGI--NLSExpressionMammalianMutationN57GPromoterCMVAvailable SinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
PET-HypaSpCas9-NLS-6xHis
Plasmid#207384PurposeExpression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cellsDepositorInsertHypaSpCas9
UseCRISPRTags6xHisExpressionBacterialMutationN692A, M694A, Q695A, H698APromoterT7Available SinceOct. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
PET-HeFSpCas9-NLS-6xHis
Plasmid#207391PurposeExpression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cellsDepositorInsertHeFSpCas9
UseCRISPRTags6xHisExpressionBacterialMutationN497A, R661A, Q695A, K848A, Q926A, K1003A, R1060APromoterT7Available SinceSept. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
PET-evoSpCas9-NLS-6xHis
Plasmid#207389PurposeExpression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cellsDepositorInsertevoSpCas9
UseCRISPRTags6xHisExpressionBacterialMutationM495V, Y515N, K526E, R661QPromoterT7Available SinceSept. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
NLS-tdPCP-GFP-VP16
Plasmid#183935PurposePCP tandem dimer binding to PP7 RNA stem loops coupled to VP16 activation domain; labeled with GFPDepositorInserttdPCP-GFP-VP16 (UL48 Synthetic)
TagsNLSExpressionMammalianMutationnonePromoterCMV (TATA box removed)Available SinceJune 13, 2022AvailabilityAcademic Institutions and Nonprofits only