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Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 7,127 results for: trac

Showing: 2961 - 2980 of 7127 results

  2. pB80-SSPB(micro)-mVenus-GCN4-ppKin14VIb(861-1321)_P2A_iLID-mCherry-RAB11

    Plasmid
    #174645
    Purpose
    Optogenetic coupling of RAB11 to tetramerized moss kinesin-14 to induce retrograde transport of recycling endosomes
    Depositor
    Lukas Kapitein
    Article
    Nijenhuis et al J Cell Biol. 2020 Apr 6;219(4). pii: 133
    Insert
    SSPB(micro)-mVenus-GCN4-ppKin14VIb(861-1321)-P2A-ILID-mCherry-RAB11
    Use
    Tags
    SSPB(micro)-Venus and iLID-mCherry
    Expression
    Mammalian
    Mutation
    SSPB:Arg73Gln; mVenus: Met1Del, Thr154Met; ppKin1…
    Promoter
    Chicken beta-actin
    Available since
    Nov. 9, 2021
    Availability
    Academic Institutions and Nonprofits only
    174645-plasmid-map-sequence-id-347047

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  3. PX458 eSp(1.1) V3

    Plasmid
    #226963
    Purpose
    CBh-eSpCas9(1.1)-2A-GFP, and hU6-sgRNA (Sp) with a BbsI golden gate cloning backbone for sgRNA. sgRNA uses 3TC scaffold for increased sgRNA expression.
    Depositor
    Paul Thomas
    Article
    Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 30, 2024
    Availability
    Academic Institutions and Nonprofits only
    226963-plasmid-map-sequence-id-450822

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  4. pcDNA5/FRT/TO-GAlphasS-RLuc8

    Plasmid
    #140980
    Purpose
    Encodes a G alpha subunit (GNAS2) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation
    Depositor
    Bryan Roth
    Article
    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/
    Insert
    GAlphasS-RLuc8
    Use
    Luciferase
    Tags
    Expression
    Mammalian
    Mutation
    RLuc8 and flanking SGGGGS linkers have been inser…
    Promoter
    CMV
    Available since
    June 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    140980-plasmid-map-sequence-id-300680

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  5. AAV-TRE-NS1-dTomato

    Plasmid
    #175278
    Purpose
    AAV vector mediating inducible bicistronic expression of NS1 gene of YFV-17D and dTomato
    Depositor
    Wei Xu
    Article
    Li et al Nat Methods. 2021 Nov 25. pii: 10.1038/s
    Insert
    NS1; dTomato (POLY Yellow fever virus strain 17D)
    Use
    AAV
    Tags
    dTomato (from bidirectional promoter)
    Expression
    Mutation
    Promoter
    bidirectional TRE promoter
    Available since
    Dec. 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    175278-plasmid-map-sequence-id-349702

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  8. pLenti4/TO-hEPO

    Plasmid
    #50437
    Purpose
    express human erythropoietin under the control of tetracycline-regulatable CMV promoter using lentiviral delivery system
    Depositor
    Juan Melero-Martin
    Article
    Lin et al Blood. 2011 Nov 17;118(20):5420-8. doi:
    Insert
    EPO (EPO Human)
    Use
    Lentiviral; Tetracycline-regulatable (t-rex)
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV/TO
    Available since
    Jan. 15, 2014
    Availability
    Academic Institutions and Nonprofits only

  10. GLuc-PM-Venus

    Plasmid
    #164785
    Purpose
    Gaussia luciferase (GLucM23) targeted to the extracellular surface of the cell membrane and intracellularly labeled with monomeric Venus fluorescent protein
    Depositor
    Laszlo Hunyady
    Article
    Toth et al J Biol Chem. 2021 Feb 2:100366. doi: 10.
    Insert
    Gaussia luciferase fused with the transmembrane domain of the platelet-derived growth factor receptor beta and Venus (PDGFRB Gaussia princeps, Human)
    Use
    Tags
    GLuc and mVenus
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    May 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    164785-plasmid-map-sequence-id-326677

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  12. PL-5LTR-GW-A

    Plasmid
    #138480
    Purpose
    For expression of ribozyme-flanked sgRNA after the Gateway (GW) LR cloning.
    Depositor
    Akitsu Hotta
    Article
    Gee et al Nat Commun. 2020 Mar 13;11(1):1334. doi:
    Insert
    Gateway attR1/R2 cassette with chloramphenicol resistance and ccdB genes
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 23, 2020
    Availability
    Academic Institutions and Nonprofits only
    138480-plasmid-map-sequence-id-275574

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  13. SaCas9v3-Puro

    Plasmid
    #178813
    Purpose
    SaCas9 with 2A-Puro, and a cloning backbone for sgRNA. sgRNA scaffold seqeuence has been modified for increased sgRNA expression (v3).
    Depositor
    Paul Thomas
    Article
    Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Cbh (Cas9-2A Puro) and U6 (gRNA)
    Available since
    Aug. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    178813-plasmid-map-sequence-id-354841

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Showing: 2961 - 2980 of 7127 results