We narrowed to 6,950 results for: ust

1. 3XHalo_GFP_TRF1#1

   Plasmid

   #164644

   Purpose

   tag TRF1 with GFP and Halo

   Depositor

   Huaiying Zhang, Michael Lampson

   Article

   Zhang et al Mol Biol Cell. 2020 Aug 15;31(18):2048-2

   Insert

   TRF1 (TERF1 Human)
   

   Use

   Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   March 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCAG_iPE-C

   Plasmid

   #214735

   Purpose

   encodes iPE-C prime editor (with MMLV-RT(H8Y, D200N, T306K, W313F, T330P, L603W) fused to the C terminus of nCas9-N863A) driven by a CAG promoter

   Depositor

   Gil Westmeyer

   Article

   Truong et al Nat Methods. 2024 Feb 1. doi: 10.1038/s4

   Insert

   CAG_iPE-C_bGH
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCAG_iPE-N

   Plasmid

   #214734

   Purpose

   encodes iPE-N prime editor (with MMLV-RT(H8Y, D200N, T306K, W313F, T330P, L603W) fused to the N terminus of nCas9-H840A) driven by a CAG promoter

   Depositor

   Gil Westmeyer

   Article

   Truong et al Nat Methods. 2024 Feb 1. doi: 10.1038/s4

   Insert

   CAG_iPE-N_bGH
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pC4-RhE-FRB-Fis1

   Plasmid

   #68056

   Purpose

   express Fis1 tail-anchored FRB for heterodimerization with FKBP in mammalian cells

   Depositor

   Richard Youle

   Article

   Narendra et al Autophagy. 2010 Nov;6(8):1090-106.

   Insert

   Fis1 (FIS1 Human)
   

   Use

   Tags

   FRB

   Expression

   Mammalian

   Mutation

   Fis1 tail (aa 93-152)

   Promoter

   Available since

   Aug. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pW5Y_Apr

   Plasmid

   #158211

   Purpose

   BAC-based, lacUV5 regulon and Cre recombinase in loxP and lox5171 sites

   Depositor

   Yasuo Yoshikuni

   Article

   Wang et al Nat Microbiol. 2019 Dec;4(12):2498-2510.

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. A*24:02:01

   Plasmid

   #203295

   Purpose

   Overexpress HLA allele

   Depositor

   Derin Keskin, Catherine Wu

   Article

   Sarkizova et al Nat Biotechnol. 2020 Feb;38(2):199-209.

   Insert

   A*24:02:01 (HLA-A Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. Yiplac211 proGAL1-TsCC(A)-RGG-GFP-RGG-RGG

   Plasmid

   #176395

   Purpose

   Expresses GFP tagged triple RGG scaffold with N-terminal TsCC(A) coiled coil. Expression induced in media containing galactose.

   Depositor

   Matthew Good

   Article

   Garabedian et al Nat Chem Biol. 2021 Sep;17(9):998-1007.

   Insert

   GAL1 promoter, TsCC(A)-RGG-GFP-RGG-RGG
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   GAL1

   Available since

   July 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. Foxf1 HA pShuttle

   Plasmid

   #122120

   Purpose

   Expression of Foxf1 in mammalian cells

   Depositor

   Paul Herring

   Article

   Hoggatt et al J Biol Chem. 2013 Oct 4;288(40):28477-87

   Insert

   Foxf1 (Foxf1 Mouse)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. Flag-ZDHHC7

   Plasmid

   #231656

   Purpose

   Expresses human ZDHHC7 protein

   Depositor

   Chunhong Ma

   Article

   Zhang et al Sci Immunol. 2024 Nov 15;9(101):eadp7302

   Insert

   ZDHHC7 (ZDHHC7 Human)
   

   Use

   Tags

   3×Flag Tag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 27, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. Flag-ZDHHC3

    Plasmid

    #231652

    Purpose

    Expresses human ZDHHC3 protein

    Depositor

    Chunhong Ma

    Article

    Zhang et al Sci Immunol. 2024 Nov 15;9(101):eadp7302

    Insert

    DHHC3 (ZDHHC3 Human)
    

    Use

    Tags

    3×Flag Tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-TRE-DIO-GCaMP6s

    Plasmid

    #183809

    Purpose

    his plasmid is for use with neuronal cell-type selective activity tagging. The GCaMP6 expression requires both neural activity and Cre recombinase.

    Depositor

    William Wisden

    Article

    Yu et al Science. 2022 Jul;377(6601):63-72. doi:

    Insert

    AAV transgene TetO promoter-DIO/flex-GCaMP6
    

    Use

    AAV, Cre/Lox, and Mouse Targeting

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3.1 T21R-vhhGFP4-T2A-mCherry

    Plasmid

    #225587

    Purpose

    Mammalian expression vector for TRIM21 RING-vhhGFP4 anti-GFP degrader with a self-cleaving T2A-mCherry fluorescent expression reporter

    Depositor

    William McEwan

    Article

    Benn et al Science. 2024 Aug 30;385(6712):1009-1016

    Insert

    T21R-vhhGFP4-T2A-mCherry
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. Gβ-2A-cpV-Gγ2-IRES-Gαi3-mTq2

    Plasmid

    #69625

    Purpose

    A FRET sensor for Galphai3 activation, encoded on a single plasmid.

    Depositor

    Dorus Gadella

    Article

    van Unen et al PLoS One. 2016 Jan 22;11(1):e0146789. do

    Inserts

    Gbeta-2A-cpV-Ggamma2
    Galphai3-mTurquoise2
    

    Use

    Tags

    ciruclar permutated Venus (cp173V) and mTurquoise…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and CMV (IRES)

    Available since

    Jan. 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. CRY2olig-mCh-CLC

    Plasmid

    #60035

    Purpose

    Expresses a fusion of CRY2olig (PHR, E490G) with mCherry and mouse clathrin light chain

    Depositor

    Chandra Tucker

    Article

    Taslimi et al Nat Commun. 2014 Sep 18;5:4925. doi: 10.

    Insert

    CRY2-mCh-Clathrin Light Chain (CRY2 Mustard Weed, Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    M354I, E490G CRY2, A9D*

    Promoter

    CMV

    Available since

    Oct. 30, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. AllCB shRNA-1

    Plasmid

    #160065

    Purpose

    Knock Down all Collybistin isoforms

    Depositor

    Angel de Blas

    Article

    George et al J Neurochem. 2021 May;157(4):1032-1051.

    Insert

    ARHGEF9 (Arhgef9 Rat)
    

    Use

    RNAi

    Tags

    Expression

    Mutation

    Promoter

    mouse U6 promoter

    Available since

    Feb. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. psigma2-EGFP

    Plasmid

    #53610

    Purpose

    Expresses sigma2 of adaptor protein of AP2

    Depositor

    Tom Kirchhausen

    Article

    Kural et al Cell Rep. 2012 Nov 29;2(5):1111-9. doi:

    Insert

    sigma2 (Ap2s1 Rat)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. B*40:01:01

    Plasmid

    #203257

    Purpose

    Overexpress HLA allele

    Depositor

    Derin Keskin, Catherine Wu

    Article

    Sarkizova et al Nat Biotechnol. 2020 Feb;38(2):199-209.

    Insert

    B*40:01:01 (HLA-B Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCD565

    Plasmid

    #153027

    Purpose

    dxCas9(3.7), MCP-SoxS(R93A/S101A), J306 scRNA

    Depositor

    Jesse Zalatan

    Article

    Fontana et al Nat Commun. 2020 Apr 1;11(1):1618. doi:

    Insert

    dxCas9(3.7), MCP-SoxS(R93A/S101A), J306 scRNA
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    SoxS has R93A and S101A mutations

    Promoter

    Available since

    Aug. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. B*58:01:01

    Plasmid

    #203261

    Purpose

    Overexpress HLA allele

    Depositor

    Derin Keskin, Catherine Wu

    Article

    Sarkizova et al Nat Biotechnol. 2020 Feb;38(2):199-209.

    Insert

    B*58:01:01 (HLA-B Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pH-Ubi-miniSdd7-nCas9-PBE

    Plasmid

    #204856

    Purpose

    For cytosine base editing using miniSdd7 in monocotyledon plants (Agrobacterium-mediated transformation)

    Depositor

    Caixia Gao

    Article

    Huang et al Cell. 2023 Jul 20;186(15):3182-3195.e14.

    Insert

    miniSdd7-nSpCas9(D10A)-UGI
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    D10A in SpCas9

    Promoter

    ZmUbi-1, OsU3, 35S

    Available since

    Aug. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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