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pETM-60-NcQDE2 _506-938-R895A_M

Plasmid

#146967

Purpose

Bacterial Expression of NcQde-2_506-938-R895A

Depositor

Elisa Izaurralde

Article

Boland et al Proc Natl Acad Sci U S A. 2011 Jun 28;10

Insert

NcQde-2_506-938-R895A

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pETM-60-NcQDE2 _506-938-Y595L_M

Plasmid

#146968

Purpose

Bacterial Expression of NcQde-2_506-938-Y595L

Depositor

Elisa Izaurralde

Article

Boland et al Proc Natl Acad Sci U S A. 2011 Jun 28;10

Insert

NcQde-2_506-938-Y595L

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmAGO1-del835-886-GGSG_M

Plasmid

#146948

Purpose

Insect Expression of DmAGO1-del835-886-GGSG

Depositor

Elisa Izaurralde

Article

Boland et al Proc Natl Acad Sci U S A. 2011 Jun 28;10

Insert

DmAGO1-del835-886-GGSG (AGO1 Fly)

Expression

Insect

Mutation

variation with 2 non silent mutations V315A and H…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmAGO1-del835-886-GSG_M

Plasmid

#146949

Purpose

Insect Expression of DmAGO1-del835-886-GSG

Depositor

Elisa Izaurralde

Article

Boland et al Proc Natl Acad Sci U S A. 2011 Jun 28;10

Insert

DmAGO1-del835-886-GSG (AGO1 Fly)

Expression

Insect

Mutation

variation with 2 non silent mutations V315A and H…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmAGO1-D661E-siRNAres_J

Plasmid

#146713

Purpose

Insect Expression of DmAGO1-D661E-siRNAres

Depositor

Elisa Izaurralde

Article

Boland et al Proc Natl Acad Sci U S A. 2011 Jun 28;10

Insert

DmAGO1-D661E-siRNAres (AGO1 Fly)

Expression

Insect

Mutation

variation with 2 non silent mutations compared to…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmAGO1-D661K-siRNAres_J

Plasmid

#146714

Purpose

Insect Expression of DmAGO1-D661K-siRNAres

Depositor

Elisa Izaurralde

Article

Boland et al Proc Natl Acad Sci U S A. 2011 Jun 28;10

Insert

DmAGO1-D661K-siRNAres (AGO1 Fly)

Expression

Insect

Mutation

variation with 2 non silent mutations compared to…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmAGO1-K657E-siRNAres_J

Plasmid

#146715

Purpose

Insect Expression of DmAGO1-K657E-siRNAres

Depositor

Elisa Izaurralde

Article

Boland et al Proc Natl Acad Sci U S A. 2011 Jun 28;10

Insert

DmAGO1-K657E-siRNAres (AGO1 Fly)

Expression

Insect

Mutation

variation with 2 non silent mutations compared to…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmAGO1-Y653L-siRNAres_J

Plasmid

#146716

Purpose

Insect Expression of DmAGO1-Y653L-siRNAres

Depositor

Elisa Izaurralde

Article

Boland et al Proc Natl Acad Sci U S A. 2011 Jun 28;10

Insert

DmAGO1-Y653L-siRNAres (AGO1 Fly)

Expression

Insect

Mutation

variation with 2 non silent mutations compared to…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pETM-60-NcQDE2_485-938_H

Plasmid

#146457

Purpose

Bacterial Expression of NcQDE2_485-938

Depositor

Elisa Izaurralde

Article

Boland et al EMBO Rep. 2010 Jul;11(7):522-7. doi: 10.

Insert

NcQDE2_485-938

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pETM-60-NcQDE2_506-938_H

Plasmid

#146458

Purpose

Bacterial Expression of NcQDE2_506-938

Depositor

Elisa Izaurralde

Article

Boland et al EMBO Rep. 2010 Jul;11(7):522-7. doi: 10.

Insert

NcQDE2_506-938

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pETM-60-NcQDE2_517-938_H

Plasmid

#146459

Purpose

Bacterial Expression of NcQDE2_517-938

Depositor

Elisa Izaurralde

Article

Boland et al EMBO Rep. 2010 Jul;11(7):522-7. doi: 10.

Insert

NcQDE2_517-938

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmAGO1-K690A_M

Plasmid

#146957

Purpose

Insect Expression of DmAGO1-K690A

Depositor

Elisa Izaurralde

Article

Boland et al Proc Natl Acad Sci U S A. 2011 Jun 28;10

Insert

DmAGO1-K690A (AGO1 Fly)

Expression

Insect

Mutation

variation with 2 non silent mutations V315A and H…

Available Since

Feb. 24, 2022

Availability

Academic Institutions and Nonprofits only

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psiCheck2 6xmut UTR

Plasmid

#40764

Purpose

Renilla luciferase reporter containing siRNA target sites

Depositor

Phillip Zamore

Article

Broderick et al RNA. 2011 Oct;17(10):1858-69. Epub 2011

Insert

6 copies (3 site expanded) of the CXCR4 small RNA target site

Use

Luciferase

Tags

luciferase

Promoter

TK/SV40

Available Since

Sept. 27, 2013

Availability

Academic Institutions and Nonprofits only
pSCALPS-Flag-Miwi-EGFP

Plasmid

#212575

Purpose

Lentiviral expression of Flag-tagged mouse Miwi in mammalian cells

Depositor

Phillip Zamore

Article

Arif et al Nature. 2022 Aug;608(7923):618-625. doi:

Insert

Miwi (Piwil1 Mouse)

Use

Lentiviral

Tags

5x Flag and SNAP tag

Expression

Mammalian

Promoter

SFFV

Available Since

May 16, 2024

Availability

Academic Institutions and Nonprofits only

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pIRESneo-FLAG/HA-FL

Plasmid

#215839

Purpose

Expression of FLAG/HA tagged firefly luciferase (FL)

Depositor

Kumiko Ui-Tei

Article

Nishi et al Nucleic Acids Res. 2015 Nov 16;43(20):98

Insert

FLAG/HA tagged firefly luciferase (FL)

Expression

Mammalian

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pSCALPS-Flag-Mili-EGFP

Plasmid

#212576

Purpose

Lentiviral expression of Flag-tagged mouse Mili in mammalian cells

Depositor

Phillip Zamore

Article

Arif et al Nature. 2022 Aug;608(7923):618-625. doi:

Insert

Mili (Piwil2 Mouse)

Use

Lentiviral

Tags

5x Flag and SNAP tag

Expression

Mammalian

Promoter

SFFV

Available Since

March 20, 2024

Availability

Academic Institutions and Nonprofits only

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MSCV-Puro-FH-AGO2-T830E

Plasmid

#91996

Purpose

Expresses FLAG-HA-AGO2 (T830E)

Depositor

Joshua Mendell

Article

Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

Insert

AGO2 (AGO2 Human)

Use

Retroviral

Tags

FLAG-HA

Expression

Mammalian

Mutation

T830E

Available Since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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MSCV-Puro-FH-AGO2-S834E

Plasmid

#91998

Purpose

Expresses FLAG-HA-AGO2 (S834E)

Depositor

Joshua Mendell

Article

Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

Insert

AGO2 (AGO2 Human)

Use

Retroviral

Tags

FLAG-HA

Expression

Mammalian

Mutation

S834E

Available Since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pLJM1-FH-AGO2-S828A

Plasmid

#91982

Purpose

Expresses FLAG-HA-AGO2 (S828A)

Depositor

Joshua Mendell

Article

Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

Insert

AGO2 (AGO2 Human)

Use

Lentiviral

Tags

FLAG-HA

Expression

Mammalian

Mutation

S828A

Available Since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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MSCV-Puro-FH-AGO2-S824A

Plasmid

#91987

Purpose

Expresses FLAG-HA-AGO2 (S824A)

Depositor

Joshua Mendell

Article

Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

Insert

AGO2 (AGO2 Human)

Use

Retroviral

Tags

FLAG-HA

Expression

Mammalian

Mutation

S824A

Available Since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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