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pET28b+R3(R112C/T180C)C

Plasmid

#108194

Purpose

Expresses PdR-PCNA3(R112C/T180C)-P450cam fusion protein in E. coli

Depositor

Teruyuki Nagamune

Article

Haga et al Biotechnol J. 2018 Dec;13(12):e1800088.

Insert

PdR-PCNA3(R112C/T180C)-P450cam fusion protein

Use

Tags

His6 tag

Expression

Bacterial

Mutation

R112C/T180C(PCNA3)

Promoter

T7 promoter

Available since

April 24, 2018

Availability

Academic Institutions and Nonprofits only

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pET28b+3(R112C/T180C)C

Plasmid

#108193

Purpose

Expresses PCNA3(R112C/T180C)-P450cam fusion protein in E. coli

Depositor

Teruyuki Nagamune

Article

Haga et al Biotechnol J. 2018 Dec;13(12):e1800088.

Insert

PCNA3(R112C/T180C)-P450cam fusion protein

Use

Tags

His6 tag

Expression

Bacterial

Mutation

R112C/T180C(PCNA3)

Promoter

T7 promoter

Available since

April 24, 2018

Availability

Academic Institutions and Nonprofits only

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pET15b+2(L171C)X

Plasmid

#108192

Purpose

Expresses PdR-PCNA2(L171C)-PdX fusion protein in E. coli

Depositor

Teruyuki Nagamune

Article

Haga et al Biotechnol J. 2018 Dec;13(12):e1800088.

Insert

PCNA2(L171C)-PdX fusion protein

Use

Tags

His6 tag

Expression

Bacterial

Mutation

L171C (PCNA2); C73S/C85S (PdX)

Promoter

T7 promoter

Available since

April 18, 2018

Availability

Academic Institutions and Nonprofits only

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pET15b+R2(L171C)X

Plasmid

#108191

Purpose

Expresses PdR-PCNA2(L171C)-PdX fusion protein in E. coli

Depositor

Teruyuki Nagamune

Article

Haga et al Biotechnol J. 2018 Dec;13(12):e1800088.

Insert

PdR-PCNA2(L171C)-PdX fusion protein

Use

Tags

His6 tag

Expression

Bacterial

Mutation

L171C (PCNA2); C73S/C85S (PdX)

Promoter

T7 promoter

Available since

April 18, 2018

Availability

Academic Institutions and Nonprofits only

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pET15b+1(G108C)X

Plasmid

#108189

Purpose

Expresses PCNA1(G108C)-PdX fusion protein in E. coli

Depositor

Teruyuki Nagamune

Article

Haga et al Biotechnol J. 2018 Dec;13(12):e1800088.

Insert

PCNA1(G108C)-PdX fusion protein

Use

Tags

His6 tag

Expression

Bacterial

Mutation

G108C (PCNA1); C73S/C85S (PdX)

Promoter

T7 promoter

Available since

April 17, 2018

Availability

Academic Institutions and Nonprofits only

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CD-MPR-GFP-RUSH

Plasmid

#202797

Purpose

RUSH cargo: Str-KDEL_IRES_SBP-EGFP-CD-MPR

Depositor

Juan Bonifacino

Article

Chen et al J Cell Biol. 2017 Dec 4;216(12):4141-415

Insert

M6PR (M6PR Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Changed 40 K (AAA) with R (AGA)

Promoter

CMV

Available since

Aug. 18, 2023

Availability

Academic Institutions and Nonprofits only

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CD-MPR-mCherry-RUSH

Plasmid

#202798

Purpose

RUSH cargo: Str-KDEL_IRES_SBP-mCherry-CD-MPR

Depositor

Juan Bonifacino

Article

Chen et al J Cell Biol. 2017 Dec 4;216(12):4141-415

Insert

M6PR (M6PR Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Changed 40 K (AAA) with R (AGA)

Promoter

CMV

Available since

Aug. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pCS2-mkgDUX4mal*leu*

Plasmid

#21173

Depositor

Stephen Tapscott

Article

Snider et al Hum Mol Genet. 2009 Apr 9. ():.

Insert

DUX4 (DUX4 Human)

Use

Tags

Expression

Bacterial and Mammalian

Mutation

First point mutation at nucleotide 5114 (numberin…

Promoter

Available since

Aug. 28, 2009

Availability

Academic Institutions and Nonprofits only
pcDNA-zeo: alpha6mGFP

Plasmid

#35611

Purpose

mammalian expression of mouse Alpha6 (Chrna6) with internal mEGFP tag

Depositor

Henry Lester

Article

Xiao et al Biochem Pharmacol. 2011 Oct 15;82(8):852

Insert

nAChr alpha6mEGFP (Chrna6 Mouse)

Use

Tags

mEGFP inserted after A405 with AGA linker on eith…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 25, 2017

Availability

Academic Institutions and Nonprofits only

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FgH1tUTG

Plasmid

#70183

Purpose

Inducible expression of guide RNA with fluorescent GFP reporter

Depositor

Marco Herold

Article

Aubrey et al Cell Rep. 2015 Mar 3;10(8):1422-32. doi:

Insert

H1-Tet-sgrna cassette

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only

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Circular 200,100 with interspersed loops (SMAD4)

Plasmid

#180191

Purpose

AAV vector carrying a guide RNA targeting the human SMAD4 mRNA

Depositor

Prashant Mali

Article

Katrekar et al Nat Biotechnol. 2022 Feb 10. pii: 10.103

Insert

Circular 200,100 guide RNA with interspersed loops

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Human U6

Available since

April 4, 2022

Availability

Academic Institutions and Nonprofits only

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pET15b+C3(R112C/T180C)X

Plasmid

#108196

Purpose

Expresses P450cam-PCNA3(R112C/T180C)-PdX fusion protein in E. coli

Depositor

Teruyuki Nagamune

Article

Haga et al Biotechnol J. 2018 Dec;13(12):e1800088.

Insert

P450cam-PCNA3(R112C/T180C)-PdX fusion protein

Use

Tags

His6 tag

Expression

Bacterial

Mutation

R112C/T180C(PCNA3); C73S/C85S (PdX)

Promoter

T7 promoter

Available since

April 17, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA Myc DBC1

Plasmid

#35096

Depositor

Osamu Hiraike

Article

Hiraike et al Br J Cancer. 2010 Mar 16;102(6):1061-7.

Insert

deleted in breast cancer 1 (CCAR2 Human)

Use

Tags

Myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 24, 2012

Availability

Academic Institutions and Nonprofits only

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Circular 200,100 with interspersed loops (FANCC)

Plasmid

#180192

Purpose

AAV vector carrying a guide RNA targeting the human FANCC mRNA

Depositor

Prashant Mali

Article

Katrekar et al Nat Biotechnol. 2022 Feb 10. pii: 10.103

Insert

Circular 200,100 guide RNA with interspersed loops

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Human U6

Available since

May 17, 2022

Availability

Academic Institutions and Nonprofits only

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pSLQ7534 pHR (hU6-crSURF1-EFS-PuroR-WPRE)

Plasmid

#214878

Purpose

Lentiviral vector encoding RfxCas13d targeting SURF1 guide array

Depositor

Stanley Qi

Article

Tieu et al Cell. 2024 Feb 29;187(5):1278-1295.e20.

Insert

hU6-crSURF1-EFS-PuroR-WPRE

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

April 4, 2024

Availability

Academic Institutions and Nonprofits only

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pLKO-shSmad2

Plasmid

#37051

Depositor

Sam Thiagalingam

Article

Papageorgis et al Cancer Res. 2010 Feb 1;70(3):968-78. Epu

Insert

Smad2 (SMAD2 Human)

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Jan. 10, 2013

Availability

Academic Institutions and Nonprofits only
pcDNA3 FLAG CEP68

Plasmid

#236463

Purpose

transient overexpression of Cep68 in mammalian cells

Depositor

Michele Pagano

Article

Pagan et al Nat Cell Biol. 2015 Jan;17(1):31-43. doi

Insert

Cep68 (CEP68 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 20, 2025

Availability

Academic Institutions and Nonprofits only

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pLKO.1-shDsup

Plasmid

#90024

Purpose

To establish Dsup knock down cells

Depositor

Takekazu Kunieda

Article

Hashimoto et al Nat Commun. 2016 Sep 20;7:12808. doi: 10

Insert

Dsup

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 4, 2017

Availability

Academic Institutions and Nonprofits only

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pLCHKO_PDPR_exon_deletion_3

Plasmid

#155067

Purpose

Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA for deletion of PDPR exon using SpCas9 and LbCas12a nucleases (CHyMErA system)

Depositor

Benjamin Blencowe, Jason Moffat

Article

Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

Insert

(hg)RNA for deletion of PDPR exon using SpCas9 and LbCas12a nucleases

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Aug. 12, 2020

Availability

Academic Institutions and Nonprofits only

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Circular 200,100 (RAB7A)

Plasmid

#170118

Purpose

AAV vector carrying a guide RNA targeting the human RAB7A mRNA

Depositor

Prashant Mali

Article

Katrekar et al Nat Biotechnol. 2022 Feb 10. pii: 10.103

Insert

Circular 200,100 guide RNA (RAB7A Human)

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Human U6

Available since

Feb. 11, 2022

Availability

Academic Institutions and Nonprofits only

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