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pCS2+-wbp2nlY55F

Plasmid

#172466

Purpose

expresses protein with one aa substitution to affect potential phosphorylation in ITSM domain

Depositor

Sally Moody

Article

Marchak et al Dev Biol. 2017 Sep 1;429(1):213-224. doi

Insert

wbp2nlY55F

Use

Xenopus expression

Tags

none

Mutation

changed Tyrosine at aa55 to Phenylalanine

Promoter

SP6

Available Since

Sept. 15, 2021

Availability

Academic Institutions and Nonprofits only

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pCS2+-wbp2nl

Plasmid

#172463

Purpose

expresses wild-type protein when injected into embryos

Depositor

Sally Moody

Article

Marchak et al Dev Biol. 2017 Sep 1;429(1):213-224. doi

Insert

wbp2nl (wbp2nl.L Frog)

Use

Xenopus expression

Tags

none

Mutation

none

Promoter

SP6

Available Since

Sept. 15, 2021

Availability

Academic Institutions and Nonprofits only

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pGEX-4T-1-Xlrbpa-dsRBD(+6)

Plasmid

#171548

Purpose

Encoding part of the Xenopus Xlrbpa-2 protein from M120 to L202

Depositor

Yan Zeng

Article

Zhang et al Acta Biochim Biophys Sin (Shanghai). 202

Insert

Xlrbpa (prkra.L Frog)

Expression

Bacterial

Mutation

a six amino acid_TRLTEG_insertion in the L1 region

Available Since

Aug. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pGEX-4T-1-Xlrbpa

Plasmid

#171547

Purpose

Encoding part of the Xenopus Xlrbpa-2 protein from M120 to L202

Depositor

Yan Zeng

Article

Zhang et al Acta Biochim Biophys Sin (Shanghai). 202

Insert

Xlrbpa (prkra.L Frog)

Expression

Bacterial

Available Since

Aug. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pCS2-xFAM3b.L-HA

Plasmid

#172852

Purpose

mRNA synthesis for injection into embryos

Depositor

Edward De Robertis

Article

Ding et al Proc Natl Acad Sci U S A. 2018 Sep 25;11

Insert

xFAM3B.L (fam3b.L Frog)

Tags

HA

Expression

Mammalian

Available Since

Aug. 6, 2021

Availability

Academic Institutions and Nonprofits only

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KSP_HNL

Plasmid

#129772

Purpose

Expresses Xenopus Kinesin-5 KSP motor and neck linker domain monomer for biochemical assays

Depositor

William Hancock

Insert

KSP (kif11.L Frog)

Tags

His6

Expression

Bacterial

Mutation

Truncation at 367

Available Since

Dec. 11, 2019

Availability

Academic Institutions and Nonprofits only

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p64T-XBMP2

Plasmid

#117253

Purpose

mRNA synthesis

Depositor

Gerald Thomsen

Article

Nishimatsu et al Mech Dev. 1998 Jun;74(1-2):75-88.

Insert

BMP2

Use

Xenopus expression

Available Since

July 10, 2019

Availability

Academic Institutions and Nonprofits only

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pCS2-XTbx5

Plasmid

#117247

Purpose

mRNA synthesis in situ hyb probes

Depositor

Gerald Thomsen

Article

Horb et al Development. 1999 Apr;126(8):1739-51.

Insert

Tbx5 (tbx5-b Frog)

Use

Xenopus expression

Available Since

July 9, 2019

Availability

Academic Institutions and Nonprofits only

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pCS2-BRAT

Plasmid

#117251

Purpose

mRNA synthesis

Depositor

Gerald Thomsen

Article

Horb et al Development. 1997 May;124(9):1689-98.

Insert

BRAT (vegt-a Frog)

Use

Xenopus expression

Available Since

July 9, 2019

Availability

Academic Institutions and Nonprofits only

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pCS2(+)-XSmurf1C699A

Plasmid

#117245

Purpose

mRNA synthesis

Depositor

Gerald Thomsen

Article

Zhu et al Nature. 1999 Aug 12. 400(6745):687-93.

Insert

Smurf1-C699A mutant

Use

Xenopus expression

Mutation

mutated cysteine 699 to alanine

Available Since

July 9, 2019

Availability

Academic Institutions and Nonprofits only

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pCS2(+)-XSmurf1

Plasmid

#117244

Purpose

mRNA synthesis in situ hyb probes

Depositor

Gerald Thomsen

Article

Zhu et al Nature. 1999 Aug 12. 400(6745):687-93.

Insert

Smurf1

Use

Xenopus expression

Available Since

July 9, 2019

Availability

Academic Institutions and Nonprofits only

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pCS2-BRAT-EnR

Plasmid

#117252

Purpose

mRNA synthesis

Depositor

Gerald Thomsen

Article

Horb et al Development. 1997 May;124(9):1689-98.

Insert

BRAT-EnR (vegt-a Frog)

Use

Xenopus expression

Mutation

chimeric protein with engrailed repressor domain…

Available Since

July 9, 2019

Availability

Academic Institutions and Nonprofits only

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pSP64T-BMP4

Plasmid

#117254

Purpose

mRNA synthesis

Depositor

Gerald Thomsen

Article

Nishimatsu et al Mech Dev. 1998 Jun;74(1-2):75-88.

Insert

BMP4

Use

Xenopus expression

Available Since

July 9, 2019

Availability

Academic Institutions and Nonprofits only

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pGEX-4T-3-cytXlATL2-R232Q

Plasmid

#124064

Purpose

Control protein. The R232Q mutation abolishes dimerization activity, so this construct is inactive compared to wild type cytATL

Depositor

Tom Rapoport

Article

Wang et al J Cell Biol. 2013 Dec 9;203(5):801-14. d

Insert

cytXlATL2-R232Q

Expression

Bacterial

Promoter

tac

Available Since

May 6, 2019

Availability

Academic Institutions and Nonprofits only

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pGEX-4T-3-cytXlATL2

Plasmid

#124063

Purpose

Fragment of Xenopus laevis Atlastin (cytATL). This fragment contains the GTPase domain and the three-helix bundle, but lacks the transmembrane segments and the cytosolic tail.

Depositor

Tom Rapoport

Article

Wang et al J Cell Biol. 2013 Dec 9;203(5):801-14. d

Insert

cytXIATL2

Expression

Bacterial

Promoter

tac

Available Since

May 6, 2019

Availability

Academic Institutions and Nonprofits only

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pProEx-Htb-cytATL2(R323Q)-6Gly-sfGFP

Plasmid

#124066

Purpose

E. coli. exp. and purif. of X. laevis cytATL-R232Q fragment fused to GFP in c-terminus.

Depositor

Tom Rapoport

Article

Wang et al Elife. 2016 Sep 13;5. pii: e18605. doi:

Insert

cytATL-R232Q-sfGFP

Tags

sfGFP

Expression

Bacterial

Mutation

R232Q

Promoter

trc

Available Since

May 6, 2019

Availability

Academic Institutions and Nonprofits only

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pProEx-Htb-cytATL2-6Gly-sfGFP

Plasmid

#124065

Purpose

E. coli. exp. and purif. of X. laevis cytATL fragment fused to GFP in c-terminus.

Depositor

Tom Rapoport

Article

Wang et al Elife. 2016 Sep 13;5. pii: e18605. doi:

Insert

cytATL-sfGFP

Tags

sfGFP

Expression

Bacterial

Promoter

trc

Available Since

May 6, 2019

Availability

Academic Institutions and Nonprofits only

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GST-peripherin-CTS

Plasmid

#86070

Purpose

A bacterial expression plasmid encoding peripherin ciliary targeting signal with N-terminus GST-tag.

Depositor

Lei Lu

Article

Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

Insert

Peripherin (prph2.S Frog)

Tags

GST

Expression

Bacterial

Mutation

contains the region from 317-336 of peripherin

Promoter

tac

Available Since

May 9, 2017

Availability

Academic Institutions and Nonprofits only

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x.Amylase

Plasmid

#87132

Purpose

To make in situ hybridization probe for Xenopus amylase.

Depositor

Francesca Spagnoli

Insert

Amylase

Expression

Mammalian

Available Since

March 23, 2017

Availability

Academic Institutions and Nonprofits only
pSI HA Nup53xl

Plasmid

#86000

Purpose

mammalian expression of HA tagged Xenopus laevis Nup53

Depositor

Wolfram Antonin

Article

Vollmer et al EMBO J. 2012 Oct 17;31(20):4072-84. doi:

Insert

Xenopus laevis Nup53 (nup35.L Frog)

Tags

HA

Expression

Mammalian

Mutation

codon optimzed for bacterial expression

Available Since

March 21, 2017

Availability

Academic Institutions and Nonprofits only

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