377 results

1. pCS2+-wbp2nlF127P

   Plasmid

   #174507

   Purpose

   expresses protein with one aa substitution to disrupt the alpha-helix near the PH-G domain

   Depositor

   Sally Moody

   Article

   Marchak et al Dev Biol. 2017 Sep 1;429(1):213-224. doi

   Insert

   wbp2nlF127P
   

   Use

   Xenopus expression

   Tags

   none

   Mutation

   changed Phenylalanine at aa127 to Proline

   Promoter

   SP6

   Available Since

   Sept. 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCS2+-wbp2nlT45A

   Plasmid

   #172464

   Purpose

   expresses protein with one aa substitution to affect potential phosphorylation

   Depositor

   Sally Moody

   Article

   Marchak et al Dev Biol. 2017 Sep 1;429(1):213-224. doi

   Insert

   wbp2nlT45A
   

   Use

   Xenopus expression

   Tags

   none

   Mutation

   changed Threonine at aa45 to Alanine

   Promoter

   SP6

   Available Since

   Sept. 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCS2+-wbp2nlY55F

   Plasmid

   #172466

   Purpose

   expresses protein with one aa substitution to affect potential phosphorylation in ITSM domain

   Depositor

   Sally Moody

   Article

   Marchak et al Dev Biol. 2017 Sep 1;429(1):213-224. doi

   Insert

   wbp2nlY55F
   

   Use

   Xenopus expression

   Tags

   none

   Mutation

   changed Tyrosine at aa55 to Phenylalanine

   Promoter

   SP6

   Available Since

   Sept. 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCS2+-wbp2nl

   Plasmid

   #172463

   Purpose

   expresses wild-type protein when injected into embryos

   Depositor

   Sally Moody

   Article

   Marchak et al Dev Biol. 2017 Sep 1;429(1):213-224. doi

   Insert

   wbp2nl (wbp2nl.L Frog)
   

   Use

   Xenopus expression

   Tags

   none

   Mutation

   none

   Promoter

   SP6

   Available Since

   Sept. 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pGEX-4T-1-Xlrbpa-dsRBD(+6)

   Plasmid

   #171548

   Purpose

   Encoding part of the Xenopus Xlrbpa-2 protein from M120 to L202

   Depositor

   Yan Zeng

   Article

   Zhang et al Acta Biochim Biophys Sin (Shanghai). 202

   Insert

   Xlrbpa (prkra.L Frog)
   

   Expression

   Bacterial

   Mutation

   a six amino acid_TRLTEG_insertion in the L1 region

   Available Since

   Aug. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGEX-4T-1-Xlrbpa

   Plasmid

   #171547

   Purpose

   Encoding part of the Xenopus Xlrbpa-2 protein from M120 to L202

   Depositor

   Yan Zeng

   Article

   Zhang et al Acta Biochim Biophys Sin (Shanghai). 202

   Insert

   Xlrbpa (prkra.L Frog)
   

   Expression

   Bacterial

   Available Since

   Aug. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCS2-xFAM3b.L-HA

   Plasmid

   #172852

   Purpose

   mRNA synthesis for injection into embryos

   Depositor

   Edward De Robertis

   Article

   Ding et al Proc Natl Acad Sci U S A. 2018 Sep 25;11

   Insert

   xFAM3B.L (fam3b.L Frog)
   

   Tags

   HA

   Expression

   Mammalian

   Available Since

   Aug. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. KSP_HNL

   Plasmid

   #129772

   Purpose

   Expresses Xenopus Kinesin-5 KSP motor and neck linker domain monomer for biochemical assays

   Depositor

   William Hancock

   Insert

   KSP (kif11.L Frog)
   

   Tags

   His6

   Expression

   Bacterial

   Mutation

   Truncation at 367

   Available Since

   Dec. 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. p64T-XBMP2

   Plasmid

   #117253

   Purpose

   mRNA synthesis

   Depositor

   Gerald Thomsen

   Article

   Nishimatsu et al Mech Dev. 1998 Jun;74(1-2):75-88.

   Insert

   BMP2
   

   Use

   Xenopus expression

   Available Since

   July 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCS2-XTbx5

    Plasmid

    #117247

    Purpose

    mRNA synthesis in situ hyb probes

    Depositor

    Gerald Thomsen

    Article

    Horb et al Development. 1999 Apr;126(8):1739-51.

    Insert

    Tbx5 (tbx5-b Frog)
    

    Use

    Xenopus expression

    Available Since

    July 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCS2-BRAT

    Plasmid

    #117251

    Purpose

    mRNA synthesis

    Depositor

    Gerald Thomsen

    Article

    Horb et al Development. 1997 May;124(9):1689-98.

    Insert

    BRAT (vegt-a Frog)
    

    Use

    Xenopus expression

    Available Since

    July 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCS2(+)-XSmurf1C699A

    Plasmid

    #117245

    Purpose

    mRNA synthesis

    Depositor

    Gerald Thomsen

    Article

    Zhu et al Nature. 1999 Aug 12. 400(6745):687-93.

    Insert

    Smurf1-C699A mutant
    

    Use

    Xenopus expression

    Mutation

    mutated cysteine 699 to alanine

    Available Since

    July 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCS2(+)-XSmurf1

    Plasmid

    #117244

    Purpose

    mRNA synthesis in situ hyb probes

    Depositor

    Gerald Thomsen

    Article

    Zhu et al Nature. 1999 Aug 12. 400(6745):687-93.

    Insert

    Smurf1
    

    Use

    Xenopus expression

    Available Since

    July 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCS2-BRAT-EnR

    Plasmid

    #117252

    Purpose

    mRNA synthesis

    Depositor

    Gerald Thomsen

    Article

    Horb et al Development. 1997 May;124(9):1689-98.

    Insert

    BRAT-EnR (vegt-a Frog)
    

    Use

    Xenopus expression

    Mutation

    chimeric protein with engrailed repressor domain…

    Available Since

    July 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSP64T-BMP4

    Plasmid

    #117254

    Purpose

    mRNA synthesis

    Depositor

    Gerald Thomsen

    Article

    Nishimatsu et al Mech Dev. 1998 Jun;74(1-2):75-88.

    Insert

    BMP4
    

    Use

    Xenopus expression

    Available Since

    July 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGEX-4T-3-cytXlATL2-R232Q

    Plasmid

    #124064

    Purpose

    Control protein. The R232Q mutation abolishes dimerization activity, so this construct is inactive compared to wild type cytATL

    Depositor

    Tom Rapoport

    Article

    Wang et al J Cell Biol. 2013 Dec 9;203(5):801-14. d

    Insert

    cytXlATL2-R232Q
    

    Expression

    Bacterial

    Promoter

    tac

    Available Since

    May 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pGEX-4T-3-cytXlATL2

    Plasmid

    #124063

    Purpose

    Fragment of Xenopus laevis Atlastin (cytATL). This fragment contains the GTPase domain and the three-helix bundle, but lacks the transmembrane segments and the cytosolic tail.

    Depositor

    Tom Rapoport

    Article

    Wang et al J Cell Biol. 2013 Dec 9;203(5):801-14. d

    Insert

    cytXIATL2
    

    Expression

    Bacterial

    Promoter

    tac

    Available Since

    May 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pProEx-Htb-cytATL2(R323Q)-6Gly-sfGFP

    Plasmid

    #124066

    Purpose

    E. coli. exp. and purif. of X. laevis cytATL-R232Q fragment fused to GFP in c-terminus.

    Depositor

    Tom Rapoport

    Article

    Wang et al Elife. 2016 Sep 13;5. pii: e18605. doi:

    Insert

    cytATL-R232Q-sfGFP
    

    Tags

    sfGFP

    Expression

    Bacterial

    Mutation

    R232Q

    Promoter

    trc

    Available Since

    May 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pProEx-Htb-cytATL2-6Gly-sfGFP

    Plasmid

    #124065

    Purpose

    E. coli. exp. and purif. of X. laevis cytATL fragment fused to GFP in c-terminus.

    Depositor

    Tom Rapoport

    Article

    Wang et al Elife. 2016 Sep 13;5. pii: e18605. doi:

    Insert

    cytATL-sfGFP
    

    Tags

    sfGFP

    Expression

    Bacterial

    Promoter

    trc

    Available Since

    May 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. GST-peripherin-CTS

    Plasmid

    #86070

    Purpose

    A bacterial expression plasmid encoding peripherin ciliary targeting signal with N-terminus GST-tag.

    Depositor

    Lei Lu

    Article

    Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

    Insert

    Peripherin (prph2.S Frog)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    contains the region from 317-336 of peripherin

    Promoter

    tac

    Available Since

    May 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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