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pGEX4T-1 hSlp4-a del.SHD

Plasmid

#40067

Depositor

Fernando Martin-Belmonte

Article

Galvez-Santisteban et al Nat Cell Biol. 2012 Jul 22. doi: 10.1038

Insert

hSlp4-a V21A (SYTL4 Human)

Tags

GST

Expression

Bacterial

Mutation

deleted SHD domain (1-143)

Promoter

Tac

Available Since

Oct. 16, 2012

Availability

Academic Institutions and Nonprofits only
pX330-Utf1 gRNA

Plasmid

#128842

Purpose

gRNA for targeting mouse Utf1 locus using CRISPR-cas technique

Depositor

Yossi Buganim

Article

Benchetrit et al Cell Stem Cell. 2019 Jun 6;24(6):983-994

Insert

Utf1 gRNA (Utf1 Mouse)

Use

CRISPR

Available Since

Aug. 27, 2019

Availability

Academic Institutions and Nonprofits only

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pGEX4T-1 hSlp4-a V21A

Plasmid

#40064

Depositor

Fernando Martin-Belmonte

Article

Galvez-Santisteban et al Nat Cell Biol. 2012 Jul 22. doi: 10.1038

Insert

hSlp4-a V21A (SYTL4 Human)

Tags

GST

Expression

Bacterial

Mutation

Valine 21 to Alanine

Promoter

Tac

Available Since

Oct. 16, 2012

Availability

Academic Institutions and Nonprofits only
pMXs-hs-3xHA-C12AC15A-LIN-41

Plasmid

#52725

Purpose

retroviral expression of human LIN-41 / TRIM71 containing cysteine to alanine point mutations at cysteine 12 and 15 and 3 N-terminal HA tags

Depositor

Shinya Yamanaka

Article

Worringer et al Cell Stem Cell. 2014 Jan 2;14(1):40-52.

Insert

LIN-41 (TRIM71 Human)

Use

Retroviral

Tags

3xHA

Expression

Mammalian

Mutation

changed cysteines 12 and 15 to alanines

Available Since

June 11, 2014

Availability

Academic Institutions and Nonprofits only

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pBabe-Puro-FLAG-Neuroserpin oloop

Plasmid

#58262

Purpose

Retroviral vector for expressing mutated Neuroserpin in mammalian cells

Depositor

Joan Massague

Article

Valiente et al Cell. 2014 Feb 27;156(5):1002-16. doi: 1

Insert

Neuroserpin (SERPINI1 Human)

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Mutation

Mutant of neuroserpin lacking five residues from …

Available Since

Aug. 21, 2014

Availability

Academic Institutions and Nonprofits only
pMXs-hs-3xHA-deltaBBox-LIN-41

Plasmid

#52719

Purpose

retroviral expression of human LIN-41 / TRIM71 containing deletion of the BBox domain and 3 N-terminal HA tags

Depositor

Shinya Yamanaka

Article

Worringer et al Cell Stem Cell. 2014 Jan 2;14(1):40-52.

Insert

LIN-41 (TRIM71 Human)

Use

Retroviral

Tags

3xHA

Expression

Mammalian

Mutation

deleted amino acids 194-320

Available Since

June 11, 2014

Availability

Academic Institutions and Nonprofits only

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pMXs-hs-3xHA-deltaFilamin-LIN-41

Plasmid

#52721

Purpose

retroviral expression of human LIN-41 / TRIM71 containing deletion of the Filamin domain and 3 N-terminal HA tags

Depositor

Shinya Yamanaka

Article

Worringer et al Cell Stem Cell. 2014 Jan 2;14(1):40-52.

Insert

LIN-41 (TRIM71 Human)

Use

Retroviral

Tags

3xHA

Expression

Mammalian

Mutation

deleted amino acids 483–583

Available Since

June 11, 2014

Availability

Academic Institutions and Nonprofits only

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Mouse c-myc (exons II and III)

Plasmid

#8448

Depositor

Frederic Mushinski

Article

Weissinger et al Mol Cell Biol 1993 Apr;13(4):2578-85.

Insert

Mouse c-myc (exons II and III) (Myc Mouse)

Expression

Bacterial

Mutation

Exons II and III only

Available Since

April 28, 2006

Availability

Academic Institutions and Nonprofits only
XLone-UCE intron 3' deletion

Plasmid

#242182

Purpose

Express doxycycline inducible UCE intron 3' deletion of CRNDE in mammalian cells

Depositor

Joshua Mendell

Article

Lee et al Mol Cell. 2025 Apr 17;85(8):1543-1560.e1

Insert

CRNDE UCE intron with 3' deletion (CRNDE Human)

Expression

Mammalian

Promoter

TRE3GS

Available Since

Aug. 22, 2025

Availability

Academic Institutions and Nonprofits only

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XLone-Unspliced CRNDE

Plasmid

#242177

Purpose

Express doxycycline inducible Unspliced CRNDE in mammalian cells

Depositor

Joshua Mendell

Article

Lee et al Mol Cell. 2025 Apr 17;85(8):1543-1560.e1

Insert

Unspliced CRNDE (CRNDE Human)

Expression

Mammalian

Mutation

PAM mutation (CCT to GTT)

Promoter

TRE3GS

Available Since

Aug. 22, 2025

Availability

Academic Institutions and Nonprofits only

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XLone-UCE intron

Plasmid

#242180

Purpose

Express doxycycline inducible UCE-containing intron of CRNDE in mammalian cells

Depositor

Joshua Mendell

Article

Lee et al Mol Cell. 2025 Apr 17;85(8):1543-1560.e1

Insert

CRNDE UCE intron (CRNDE Human)

Expression

Mammalian

Promoter

TRE3GS

Available Since

Aug. 20, 2025

Availability

Academic Institutions and Nonprofits only

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XLone-UCE mutant

Plasmid

#242185

Purpose

Express doxycycline inducible UCE 32S binding mutation of CRNDE in mammalian cells

Depositor

Joshua Mendell

Article

Lee et al Mol Cell. 2025 Apr 17;85(8):1543-1560.e1

Insert

CRNDE UCE mutant (CRNDE Human)

Expression

Mammalian

Mutation

ATTTTCATGGGC to TAAAAGTACCCG

Promoter

TRE3GS

Available Since

Aug. 20, 2025

Availability

Academic Institutions and Nonprofits only

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XLone-UCE

Plasmid

#242184

Purpose

Express doxycycline inducible UCE intron of CRNDE in mammalian cells

Depositor

Joshua Mendell

Article

Lee et al Mol Cell. 2025 Apr 17;85(8):1543-1560.e1

Insert

CRNDE UCE (CRNDE Human)

Expression

Mammalian

Promoter

TRE3GS

Available Since

Aug. 20, 2025

Availability

Academic Institutions and Nonprofits only

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XLone-UCE intron 5' deletion

Plasmid

#242181

Purpose

Express doxycycline inducible UCE intron 5' deletion of CRNDE in mammalian cells

Depositor

Joshua Mendell

Article

Lee et al Mol Cell. 2025 Apr 17;85(8):1543-1560.e1

Insert

CRNDE UCE intron with 5' deletion (CRNDE Human)

Expression

Mammalian

Promoter

TRE3GS

Available Since

Aug. 20, 2025

Availability

Academic Institutions and Nonprofits only

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VAMP4 KD1 mScarlet

Plasmid

#174407

Purpose

lentiviral vector for VAMP4 knock-down in rat cells, KD1 shRNA

Depositor

David Perrais

Article

Bakr et al Cell Rep. 2021 Sep 7;36(10):109678. doi:

Insert

vesicle associated membrane protein 4 (Vamp4 Rat)

Use

Lentiviral

Tags

mScarlet

Expression

Mammalian

Promoter

H1-Ubiquitin

Available Since

Aug. 6, 2025

Availability

Academic Institutions and Nonprofits only

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VAMP4 KD2 mScarlet

Plasmid

#174408

Purpose

lentiviral vector for VAMP4 knock-down in rat cells, KD2 shRNA

Depositor

David Perrais

Article

Bakr et al Cell Rep. 2021 Sep 7;36(10):109678. doi:

Insert

vesicle associated membrane protein 4 (Vamp4 Rat)

Use

Lentiviral

Tags

mScarlet

Expression

Mammalian

Promoter

H1-Ubiquitin

Available Since

Aug. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-FLAG-Rgs14

Plasmid

#236396

Purpose

Expresses FLAG-Rgs14 in mammalian cells

Depositor

John Hepler

Article

Shu et al Cell Signal. 2007 Jan;19(1):163-76. doi:

Insert

Rattus norvegicus regulator of G-protein signaling 14 (Rgs14 Rat)

Tags

FLAG

Expression

Mammalian

Available Since

May 1, 2025

Availability

Academic Institutions and Nonprofits only

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pCS2+ 3xFLAG-FEM1B C186S

Plasmid

#230995

Purpose

Transient overexpression of mouse FEM1B in mammalian cells. Mutation abolishes substrate binding.

Depositor

Michael Rape

Article

Manford et al Cell. 2020 Oct 1;183(1):46-61.e21. doi:

Insert

FEM1B (Fem1b Mouse)

Tags

3xFLAG

Expression

Mammalian

Mutation

mutated Cys186 to Ser

Available Since

Jan. 23, 2025

Availability

Academic Institutions and Nonprofits only

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pCS2+ 3xFLAG-FEM1B L597A

Plasmid

#230994

Purpose

Transient overexpression of catalytic null mouse FEM1B in mammalian cells. Mutation abolishes interaction with CUL2.

Depositor

Michael Rape

Article

Manford et al Cell. 2020 Oct 1;183(1):46-61.e21. doi:

Insert

FEM1B (Fem1b Mouse)

Tags

3xFLAG

Expression

Mammalian

Mutation

mutated Leu597 to Ala

Available Since

Jan. 23, 2025

Availability

Academic Institutions and Nonprofits only

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pCAG-RhoA*

Plasmid

#198716

Purpose

Expresses gRNA resistant RhoA under the CAG promoter in mammalian cells

Depositor

Takeshi Imai

Article

Fujimoto et al Dev Cell. 2023 Jul 24;58(14):1221-1236.e

Insert

RhoA (Rhoa Mouse)

Expression

Mammalian

Mutation

It has mutations that is resistant to the RhoA gR…

Promoter

CAG

Available Since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

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