We narrowed to 3,800 results for: golden gate

1. 3'MS2-gRNA GG backbone (MOBE1-2)

   Plasmid

   #219948

   Purpose

   Golden gate backbone for mammalian expression of Sp-gRNA with 3'-MS2

   Depositor

   Alexis Komor

   Article

   Cowan et al Nat Biotechnol. 2024 May 21. doi: 10.103

   Insert

   gRNA-MS2-3'end (S. pyogenes Cas9)
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   July 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pro::EX1-2(intΔNACs):GFP

   Plasmid

   #218570

   Purpose

   Transcriptional reporter for ARF7 promoter

   Depositor

   Anthony Bishopp

   Insert

   AT5G20730 (NPH4 Mustard Weed)
   

   Expression

   Plant

   Mutation

   Mutation genomic sequence 85nt, 86nt from TA to C…

   Available Since

   July 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pKL2-160

   Plasmid

   #219414

   Purpose

   Estradiol-inducible expression of 13-6 Zinc finger activator

   Depositor

   Ahmad Khalil

   Article

   Bragdon et al Cell. 2023 Aug 31;186(18):3810-3825.e18.

   Insert

   {pTEF1-ZEV}{pGal1(Zif268op)x6-3xFLAG-NLS-VP16-mRuby2-(42-10-WT)-PDZLigWT-tSSA1}
   

   Expression

   Yeast

   Available Since

   May 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pJET-TtrpC

   Plasmid

   #213467

   Purpose

   Vector containing the TrpC terminator for Golden Gate cloning in the Fg vector

   Depositor

   Kim Hammond-Kosack

   Article

   Darino et al Fungal Biol Biotechnol. 2024 Feb 26;11(1

   Insert

   Terminator of tryptophan synthase of Aspergillus nidulans
   

   Expression

   Bacterial

   Available Since

   May 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pJET-PgpdA

   Plasmid

   #213466

   Purpose

   Vector containing the gpdA promoter for Golden Gate cloning in the Fg vector

   Depositor

   Kim Hammond-Kosack

   Article

   Darino et al Fungal Biol Biotechnol. 2024 Feb 26;11(1

   Insert

   Promoter of the glyceraldehyde-3-phosphate dehydrogenase
   

   Expression

   Bacterial

   Available Since

   May 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pJET-PtrpC

   Plasmid

   #213465

   Purpose

   Vector containing the TrpC promoter for Golden Gate cloning in the Fg vector

   Depositor

   Kim Hammond-Kosack

   Article

   Darino et al Fungal Biol Biotechnol. 2024 Feb 26;11(1

   Insert

   Promoter of tryptophan synthase of Aspergillus nidulans
   

   Expression

   Bacterial

   Available Since

   May 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGGA-pCLV3

   Plasmid

   #210528

   Purpose

   Cloning A. thaliana CLV3 promoter as GreenGate module A

   Depositor

   Ortrun Mittelsten Scheid

   Article

   Dona et al Plant J. 2023 Jul 5. doi: 10.1111/tpj.16

   Insert

   pCLV3 (CLV3 Mustard Weed)
   

   Use

   Synthetic Biology; Golden gate compatible cloning…

   Available Since

   March 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. Modular BE4max for GG insertion

   Plasmid

   #198891

   Purpose

   Easily insert deaminase of choice into BE4max using Golden Gate cloning

   Depositor

   Reuben Harris

   Article

   Rieffer et al CRISPR J. 2023 Oct;6(5):430-446. doi: 10

   Insert

   CRISPR associated protein 9 (cas9 Streptococcus pyogenes)
   

   Tags

   NLS

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pNL2114 pARF19 GG0-GG1

   Plasmid

   #195909

   Purpose

   Level 0 pARF19 promoter with GG0 and GG1 golden gate linkers

   Depositor

   Jennifer Nemhauser

   Article

   Guiziou et al Nat Commun. 2023 Apr 3;14(1):1844. doi:

   Insert

   pARF19 (ARF19 Mustard Weed)
   

   Use

   Synthetic Biology

   Available Since

   March 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. L0-I16 tUBQ1 GG5-GG6

    Plasmid

    #195898

    Purpose

    Level 0 tUBQ1 terminator with GG5 GG6 golden gate linkers

    Depositor

    Jennifer Nemhauser

    Article

    Guiziou et al Nat Commun. 2023 Apr 3;14(1):1844. doi:

    Insert

    tUBQ1 (UBQ1 Mustard Weed)
    

    Use

    Synthetic Biology

    Available Since

    March 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. L0-I15 tUBQ1 GG4-GG6

    Plasmid

    #195897

    Purpose

    Level 0 tUBQ1 terminator with GG4 GG6 golden gate linkers

    Depositor

    Jennifer Nemhauser

    Article

    Guiziou et al Nat Commun. 2023 Apr 3;14(1):1844. doi:

    Insert

    tUBQ1 (UBQ1 Mustard Weed)
    

    Use

    Synthetic Biology

    Available Since

    March 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBBK02 pCAS-Tyr-[gRNA: BsaI GFP dropout] (SplitKanR, AmpR)

    Plasmid

    #178986

    Purpose

    Sp.Cas9 and gRNA yeast expression vector for Golden Gate pre-cloning of gRNA. Selection = SplitKanamycin resistance

    Depositor

    Vincent Martin

    Article

    Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

    Insert

    S. pyogenes Cas9
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Yeast

    Available Since

    May 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBBK10 pCAS-Tyr-[gRNA: BsaI GFP dropout] (SplitURA3,AmpR)

    Plasmid

    #178994

    Purpose

    Sp.Cas9 and gRNA yeast expression vector for Golden Gate pre-cloning of gRNA. Selection = SplitURA3

    Depositor

    Vincent Martin

    Article

    Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

    Insert

    S. pyogenes Cas9
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Yeast

    Available Since

    May 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBBK08 pCAS-Tyr-[gRNA: BsaI GFP dropout] (SplitLEU2,AmpR)

    Plasmid

    #178992

    Purpose

    Sp.Cas9 and gRNA yeast expression vector for Golden Gate pre-cloning of gRNA. Selection = SplitLEU2

    Depositor

    Vincent Martin

    Article

    Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

    Insert

    S. pyogenes Cas9
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Yeast

    Available Since

    May 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBBK06 pCAS-Tyr-[gRNA: BsaI GFP dropout] (SplitNatR,AmpR)

    Plasmid

    #178990

    Purpose

    Sp.Cas9 and gRNA yeast expression vector for Golden Gate pre-cloning of gRNA. Selection = SplitNourseothrycin resistance

    Depositor

    Vincent Martin

    Article

    Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

    Insert

    S. pyogenes Cas9
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Yeast

    Available Since

    May 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pL0-MjcDOPA5GT

    Plasmid

    #162530

    Purpose

    Mirabilis jalapa cyclo-DOPA 5-O-glucosyltransferase coding sequence in pICH41308 MoClo Golden Gate level 0 acceptor for CDS1 modules.

    Depositor

    Sam Brockington

    Article

    Timoneda et al PLoS Biol. 2021 Jul 14;19(7):e3001326. d

    Insert

    cDOPA5GT
    

    Use

    Puc19-derived

    Expression

    Bacterial

    Available Since

    July 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pL0-NbPT5bPro

    Plasmid

    #159416

    Purpose

    Nicotiana benthamiana PT5b promoter sequence (1068 bp immediately upstream of start codon) in pICH41295 MoClo Golden Gate level 0 acceptor for Pro+5U modules.

    Depositor

    Sam Brockington

    Article

    Timoneda et al PLoS Biol. 2021 Jul 14;19(7):e3001326. d

    Insert

    NbPT5b Promoter
    

    Use

    Puc19-derived

    Expression

    Bacterial

    Available Since

    July 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pL0-NbBCP1bPro

    Plasmid

    #159417

    Purpose

    Nicotiana benthamiana BCP1b promoter sequence (1231 bp immediately upstream of start codon) in pICH41295 MoClo Golden Gate level 0 acceptor for Pro+5U modules.

    Depositor

    Sam Brockington

    Article

    Timoneda et al PLoS Biol. 2021 Jul 14;19(7):e3001326. d

    Insert

    NbBCP1b Pro
    

    Use

    Puc19-derived

    Expression

    Bacterial

    Available Since

    July 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pPD173 ZF43(AAAA)i

    Plasmid

    #138833

    Purpose

    Constitutive expression of ZF43(AAAA)i under the CMV promoter.

    Depositor

    Joshua Leonard

    Article

    Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

    Insert

    ZF43(AAAA)i
    

    Use

    Synthetic Biology

    Tags

    3xFlag

    Expression

    Mammalian

    Mutation

    R6A, R15A, R43A, R71A

    Promoter

    CMV

    Available Since

    Jan. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pPD389 VPR-ZF43(AAAA)

    Plasmid

    #138826

    Purpose

    Constitutive expression of VPR-ZF43(AAAA) under the CMV promoter.

    Depositor

    Joshua Leonard

    Article

    Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

    Insert

    VPR-ZF43(AAAA)
    

    Use

    Synthetic Biology

    Tags

    3xFlag

    Expression

    Mammalian

    Mutation

    R6A, R15A, R43A, R71A

    Promoter

    CMV

    Available Since

    Jan. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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