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We narrowed to 14,223 results for: Cas9

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Showing: 3001 - 3020 of 14223 results

  3. xCas9

    Plasmid
    #138565
    Purpose
    Expresses human codon-optimized expanded PAM SpCas9 variant and blasticidin resistance: EFS promoter-xCas9-NLS-FLAG-P2A-BSD
    Depositor
    Hyongbum Kim
    Publication
    Kim et al Nat Biotechnol. 2020 Nov;38(11):1328-133
    Insert
    xCas9
    Use
    CRISPR and Lentiviral
    Tags
    BSD, FLAG, and NLS
    Expression
    Mammalian
    Mutation
    A262T, R324L, S409I, E480K, E543D, M694I, E1219V
    Promoter
    EFS
    Available since
    June 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    138565-plasmid-map-sequence-id-274797

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  5. pUC19-F4GDP9

    Plasmid
    #103077
    Purpose
    Cas9 coding gene template with optimized sequence for human codon usage
    Depositor
    Duhee Bang
    Publication
    Cho et al Nucleic Acids Res. 2018 May 18;46(9):e55
    Insert
    F4GDP9(Cas9 coding gene from Alicycliphilus denitrificans (strain DSM 14773 / CIP 107495 / K601))
    Use
    CRISPR; Cloning vector
    Mutation
    human codon-optimized
    Available since
    April 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    103077-plasmid-map-sequence-id-201717

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  12. pCAG-spCas9-SB

    Plasmid
    #169919
    Purpose
    Expression of SpCas9-SB100X transposase fusion protein to enhace site-specific DNA integration.
    Depositor
    Zhili Rong
    Publication
    Ma et al Nucleic Acids Res. 2020 Oct 9;48(18):105
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SpCas9-SB100X
    Use
    CRISPR
    Promoter
    CAG
    Available since
    June 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    169919-plasmid-map-sequence-id-336025

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  14. pBluescriptSKII+ U6-sgRNA(F+E) ACTB

    Plasmid
    #74705
    Purpose
    Encodes an sgRNA for spCas9 driven by hU6 promoter with a modified scaffold (Chen et al. Cell 2013) and a spacer targeting the 3'UTR of ACTB mRNA
    Depositor
    Eugene Yeo
    Publication
    Nelles et al Cell. 2016 Mar 16. pii: S0092-8674(16)30
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    U6 promoter driving sgRNA targeting the 3'UTR of ACTB mRNA
    Use
    CRISPR
    Expression
    Mammalian
    Available since
    April 27, 2016
    Availability
    Academic Institutions and Nonprofits only
    74705-plasmid-map-sequence-id-151422

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  19. BA31_pU.CAG.SaCas9-D10A.rBGpA

    Plasmid
    #199251
    Purpose
    Expression construct encoding the S. aureus Cas9.D10A nickase (SaCas9.D10A)
    Depositor
    Manuel Goncalves
    Publication
    Wang et al Nucleic Acids Res. 2021 Jan 25;49(2):117
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SaCas9.D10A nickase
    Use
    CRISPR
    Tags
    3XHA epitope, Nucleoplasmin NLS, and SV40 NLS
    Expression
    Mammalian
    Mutation
    D10A
    Promoter
    CAG promoter
    Available since
    April 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    199251-plasmid-map-sequence-id-391385

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Showing: 3001 - 3020 of 14223 results