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Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 8,294 results for: construct

Showing: 3001 - 3020 of 8294 results

  1. pBlue-TAL

    Plasmid
    #49401
    Purpose
    The pBlue-TAL is suitable for TALEN mutagenesis in insects. It is compatible with Golden Gate Cloning Kit. It has Esp3I cleavage sites and a T7 promoter.
    Depositor
    Michal Zurovec
    Article
    Takasu et al PLoS One. 2013 Sep 18;8(9):e73458. doi:
    Type
    Empty backbone
    Use
    TALEN
    Tags
    Expression
    Insect
    Mutation
    Promoter
    Available since
    Dec. 19, 2013
    Availability
    Academic Institutions and Nonprofits only
    49401-plasmid-map-sequence-id-73425

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  2. pGBW-m4046538

    Plasmid
    #149128
    Purpose
    Yeast Expression plasmid for SARS-CoV-2 surface glycoprotein (Spike protein)
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 S (surface glycoprotein) (S Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    Cleavable thrombin;6xHIS
    Expression
    Yeast
    Mutation
    wt
    Promoter
    pTEF1
    Available since
    May 5, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    149128-plasmid-map-sequence-id-296274

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  4. pGBW-m4137387

    Plasmid
    #149544
    Purpose
    Mammalian expression plasmid for SARS-CoV-2 surface glycoprotein (Spike protein)
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 S (surface glycoprotein) (S Severe acute respiratory syndrome coronavirus 2, Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    wt;19-aa Ctrunc
    Promoter
    CMV
    Available since
    May 14, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits

  7. pGBW-m4046494

    Plasmid
    #149137
    Purpose
    Yeast Expression plasmid for SARS-CoV-2 surface glycoprotein (Spike protein)
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 surface glycoprotein (S Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    Cleavable thrombin;6xHIS
    Expression
    Yeast
    Mutation
    Saccharomyces cerevisiae recode 1
    Promoter
    pTEF1
    Available since
    May 14, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    149137-plasmid-map-sequence-id-307325

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  10. pBabe-Kras G15A

    Plasmid
    #58898
    Purpose
    Expresses human Kras with a G15A mutation
    Depositor
    Channing Der
    Insert
    KRAS (KRAS Human)
    Use
    Retroviral
    Tags
    HA
    Expression
    Mammalian
    Mutation
    G15A mutation
    Promoter
    Available since
    Sept. 15, 2014
    Availability
    Academic Institutions and Nonprofits only

  12. NAT-TET-OFF -LEU- 4xFLAG

    Plasmid
    #182527
    Purpose
    Doxycycline-induced transcriptional regulation and Ubiquitin - LEU - 4xFLAG N-terminal tagging. Ubiquitin is cleaved off, resulting in an unstable protein with an N-terminal leucine.
    Depositor
    Rob Mitra
    Article
    Shively et al ACS Synth Biol. 2023 Jan 17. doi: 10.102
    Insert
    7 Copies of TetO Box
    Use
    Tags
    4XFLAG
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Jan. 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    182527-plasmid-map-sequence-id-358175

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  18. TDGS50

    Plasmid
    #183189
    Purpose
    TEV protease expressed under J23106 promoter; DivIVAsfGFP-TEV protease recognition site-M0050 expressed under J23116 promoter
    Depositor
    Hsiao-Chun Huang
    Article
    Hong et al ACS Synth Biol. 2021 Aug 20;10(8):1830-1
    Inserts
    DivIVA-GFP(tevR M0050)
    TEV-S219V
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    July 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    183189-plasmid-map-sequence-id-366595

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  20. pGBW-m4046464

    Plasmid
    #148970
    Purpose
    Yeast Expression plasmid for SARS-CoV-2 surface glycoprotein (Spike protein)
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 S (surface glycoprotein) (S Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    Cleavable thrombin;3xFLAG
    Expression
    Yeast
    Mutation
    wt
    Promoter
    pGAL1
    Available since
    May 14, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    148970-plasmid-map-sequence-id-297202

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Showing: 3001 - 3020 of 8294 results