Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

We narrowed to 4,045 results for: golden gate

Showing: 3001 - 3020 of 4045 results

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pSEVAb897

Plasmid

#217505

Purpose

pSEVAb (SEVA 3.1), Type IIS compatible plasmid with sfGFP under the BBa_J23119 promoter, pSEVAb89 backbone, pBR322/ROP ori

Depositor

Nico Claassens

Article

van den Brink et al ACS Synth Biol. 2025 Jun 5. doi: 10.1021

Insert

superfolder green fluorescent protein (sfGFP)

Use

Synthetic Biology

Mutation

S30R, Y39N, F64L, S65T, Q80R, F99S, N105T, Y145F,…

Available Since

July 17, 2025

Availability

Academic Institutions and Nonprofits only

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pCAD183_gCAD_pET

Plasmid

#239119

Purpose

Gorilla ACOD1 in E.coli expression vector

Depositor

Konrad Buessow

Article

Chen et al Sci Rep. 2023 Jun 26;13(1):10360. doi: 1

Insert

ACOD1

Tags

StrepTag-II and TEV site

Expression

Bacterial

Promoter

T7

Available Since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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pKG3668_pGEEC578_pShip-EFS-rtTA-P2A-iRFP720-bGH

Plasmid

#239741

Purpose

Plasmid expressing a doxycycline-inducible transcriptional activator

Depositor

Kate Galloway

Article

Peterman et al Nucleic Acids Research, Volume 53, Issue

Insert

rtTA-P2A-iRFP720

Use

Synthetic Biology

Available Since

July 10, 2025

Availability

Academic Institutions and Nonprofits only

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pKG2499_pGEEC531_pShip-CMV-mRuby2-WPRE

Plasmid

#239727

Purpose

Plasmid expressing mRuby2 with the CMV promoter and WPRE 3' UTR

Depositor

Kate Galloway

Article

Peterman et al Nucleic Acids Research, Volume 53, Issue

Insert

mRuby2

Use

Synthetic Biology

Available Since

July 10, 2025

Availability

Academic Institutions and Nonprofits only

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pKG2906_pGEEC546_pShip-EFS-tagBFP-bGH

Plasmid

#239734

Purpose

Plasmid expressing tagBFP with the EFS promoter

Depositor

Kate Galloway

Article

Peterman et al Nucleic Acids Research, Volume 53, Issue

Insert

tagBFP

Use

Synthetic Biology

Available Since

July 10, 2025

Availability

Academic Institutions and Nonprofits only

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pKG2907_pGEEC547_pShip-hPGK-tagBFP-bGH

Plasmid

#239735

Purpose

Plasmid expressing tagBFP with the hPGK promoter

Depositor

Kate Galloway

Article

Peterman et al Nucleic Acids Research, Volume 53, Issue

Insert

tagBFP

Use

Synthetic Biology

Available Since

July 10, 2025

Availability

Academic Institutions and Nonprofits only

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pKG2897_pGEEC548_pShip-UbC-tagBFP-bGH

Plasmid

#239736

Purpose

Plasmid expressing tagBFP with the UbC promoter

Depositor

Kate Galloway

Article

Peterman et al Nucleic Acids Research, Volume 53, Issue

Insert

tagBFP

Use

Synthetic Biology

Available Since

July 10, 2025

Availability

Academic Institutions and Nonprofits only

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pKG2899_pGEEC550_pShip-CMV-tagBFP-bGH

Plasmid

#239737

Purpose

Plasmid expressing tagBFP with the CMV promoter

Depositor

Kate Galloway

Article

Peterman et al Nucleic Acids Research, Volume 53, Issue

Insert

tagBFP

Use

Synthetic Biology

Available Since

July 10, 2025

Availability

Academic Institutions and Nonprofits only

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pKG2900_pGEEC551_pShip-CAG-tagBFP-bGH

Plasmid

#239738

Purpose

Plasmid expressing tagBFP with the CAG promoter

Depositor

Kate Galloway

Article

Peterman et al Nucleic Acids Research, Volume 53, Issue

Insert

tagBFP

Use

Synthetic Biology

Available Since

July 10, 2025

Availability

Academic Institutions and Nonprofits only

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pKG2023_pGEEC509_pShip-UbC-mRuby2-WPRE

Plasmid

#239715

Purpose

Plasmid expressing mRuby2 with the UbC promoter and WPRE 3' UTR

Depositor

Kate Galloway

Article

Peterman et al Nucleic Acids Research, Volume 53, Issue

Insert

mRuby2

Use

Synthetic Biology

Available Since

July 10, 2025

Availability

Academic Institutions and Nonprofits only

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pKG2116_pGEEC510_pShip-EF1a-mRuby2-bGH

Plasmid

#239716

Purpose

Plasmid expressing mRuby2 with the EF1a promoter and bGH 3' UTR

Depositor

Kate Galloway

Article

Peterman et al Nucleic Acids Research, Volume 53, Issue

Insert

mRuby2

Use

Synthetic Biology

Available Since

July 10, 2025

Availability

Academic Institutions and Nonprofits only

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pKG2117_pGEEC511_pShip-EF1a-mRuby2-SV40

Plasmid

#239717

Purpose

Plasmid expressing mRuby2 with the EF1a promoter and SV40 3' UTR

Depositor

Kate Galloway

Article

Peterman et al Nucleic Acids Research, Volume 53, Issue

Insert

mRuby2

Use

Synthetic Biology

Available Since

July 10, 2025

Availability

Academic Institutions and Nonprofits only

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pKG2118_pGEEC512_pShip-EF1a-mRuby2-WPRE

Plasmid

#239718

Purpose

Plasmid expressing mRuby2 with the EF1a promoter and WPRE 3' UTR

Depositor

Kate Galloway

Article

Peterman et al Nucleic Acids Research, Volume 53, Issue

Insert

mRuby2

Use

Synthetic Biology

Available Since

July 10, 2025

Availability

Academic Institutions and Nonprofits only

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pKG2075_pGEEC516_pShip-CAG-mRuby2-bGH

Plasmid

#239719

Purpose

Plasmid expressing mRuby2 with the CAG promoter and bGH 3' UTR

Depositor

Kate Galloway

Article

Peterman et al Nucleic Acids Research, Volume 53, Issue

Insert

mRuby2

Use

Synthetic Biology

Available Since

July 10, 2025

Availability

Academic Institutions and Nonprofits only

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pKG2076_pGEEC517_pShip-CAG-mRuby2-SV40

Plasmid

#239720

Purpose

Plasmid expressing mRuby2 with the CAG promoter and SV40 3' UTR

Depositor

Kate Galloway

Article

Peterman et al Nucleic Acids Research, Volume 53, Issue

Insert

mRuby2

Use

Synthetic Biology

Available Since

July 10, 2025

Availability

Academic Institutions and Nonprofits only

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pKG2077_pGEEC518_pShip-CAG-mRuby2-WPRE

Plasmid

#239721

Purpose

Plasmid expressing mRuby2 with the CAG promoter and WPRE 3' UTR

Depositor

Kate Galloway

Article

Peterman et al Nucleic Acids Research, Volume 53, Issue

Insert

mRuby2

Use

Synthetic Biology

Available Since

July 10, 2025

Availability

Academic Institutions and Nonprofits only

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pKG2309_pGEEC524_pShip-EFS-ZF10-NS3-p65-bGH

Plasmid

#239722

Purpose

Plasmid expressing a grazoprevir-inducible transcriptional activator

Depositor

Kate Galloway

Article

Peterman et al Nucleic Acids Research, Volume 53, Issue

Insert

ZF10-NS3-p65

Use

Synthetic Biology

Available Since

July 10, 2025

Availability

Academic Institutions and Nonprofits only

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pKG2215_pGEEC525_pShip-ZF43x6-C-mRuby2-bGH

Plasmid

#239723

Purpose

Plasmid expressing mRuby2 from a COMET promoter, Induced with pKG3666

Depositor

Kate Galloway

Article

Peterman et al Nucleic Acids Research, Volume 53, Issue

Insert

mRuby2

Use

Synthetic Biology

Available Since

July 10, 2025

Availability

Academic Institutions and Nonprofits only

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pKG2410_pGEEC526_pShip-TRE3G-mRuby2-bGH

Plasmid

#239724

Purpose

Plasmid expressing mRuby2 from the TRE3G promoter, Induced with pKG3668

Depositor

Kate Galloway

Article

Peterman et al Nucleic Acids Research, Volume 53, Issue

Insert

mRuby2

Use

Synthetic Biology

Available Since

July 10, 2025

Availability

Academic Institutions and Nonprofits only

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pKG2497_pGEEC529_pShip-CMV-mRuby2-bGH

Plasmid

#239725

Purpose

Plasmid expressing mRuby2 with the CMV promoter and bGH 3' UTR

Depositor

Kate Galloway

Article

Peterman et al Nucleic Acids Research, Volume 53, Issue

Insert

mRuby2

Use

Synthetic Biology

Available Since

July 10, 2025

Availability

Academic Institutions and Nonprofits only

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