Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

We narrowed to 6,447 results for: nls

Clear keywords
Showing: 3001 - 3020 of 6447 results

  3. pCC_06 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-dCas9NG-NLS-VPR-2A-Puro-WPRE

    Plasmid
    #139091
    Purpose
    Expresses human codon-optimized inactive SpCas9-NG fused to a transcriptional activator VPR in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a barcode downstream of sgRNA cassette.
    Depositor
    Neville Sanjana
    Article
    Legut et al Cell Rep. 2020 Mar 3;30(9):2859-2868.e5.
    Insert
    dSpCas9-NG-VPR
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Mutation
    D10A, H840A, L1111R, D1135V,G1218R, E1219F, A1322…
    Available Since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    139091-plasmid-map-sequence-id-273888

    Enlarge

  5. pUBC-SV40NLS-Pfv-Sapphire-PURO

    Plasmid
    #203654
    Purpose
    Mammalian lentiviral vector for the expression of the Sapphire-tagged nuclear localizing 40nm nucGEMs under the UBC promoter, with a puromycin selection marker.
    Depositor
    Liam Holt
    Article
    Shu T et et al bioRxiv 2022
    Insert
    PfV
    Use
    Lentiviral
    Tags
    Sapphire
    Available Since
    July 26, 2023
    Availability
    Academic Institutions and Nonprofits only
    203654-plasmid-map-sequence-id-402486

    Enlarge

  6. TV_LGR5-ires-mNeon-nls-p2a-dtr-wpre_pgk-puro

    Plasmid
    #251173
    Purpose
    Targeting vector for C-terminal knockin at LGR5 locus to enable reporting of expression by mNeonGreen and depletion of positive cells with DTR
    Depositor
    Hugo Snippert
    Insert
    ires-mNeon-nls-p2a-dtr-wpre_pgk-puro flanked by homology arms for C-terminal targeting of human LGR5 (LGR5 Human)
    Use
    CRISPR
    Tags
    ires-mNeon-nls-p2a-dtr
    Available Since
    Feb. 20, 2026
    Availability
    Academic Institutions and Nonprofits only
    251173-plasmid-map-sequence-id-500690

    Enlarge

  10. pTEE

    Plasmid
    #159748
    Purpose
    Egg cell-specific promoter-controlled expression of 3×FLAG-NLS-zCas9-NLS. Contains gRNA scaffold for insertion of target sequence (U6-26 promoter). Selection of transgenic seeds by mTomato fluoresce.
    Depositor
    Wolfgang Lukowitz
    Article
    Angulo et al bioRxiv
    Insert
    gRNA scaffold
    Use
    CRISPR
    Expression
    Plant
    Available Since
    Oct. 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    159748-plasmid-map-sequence-id-313816

    Enlarge

  11. pHygRB12

    Plasmid
    #244899
    Purpose
    hygR selection nls-Scarlet marked rapid RMCE integration vector with an attB site for RMHE
    Depositor
    Michael Nonet
    Article
    Nonet et al bioRxiv 2024.03.01.583017
    Type
    Empty backbone
    Use
    Recombination mediated cassette exchange; recombi…
    Expression
    Worm
    Available Since
    Sept. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    244899-plasmid-map-sequence-id-485995

    Enlarge

  12. pHygGB16

    Plasmid
    #244896
    Purpose
    hygR selection nls-GFP marked rapid RMCE integration vector with an attB site for RMHE
    Depositor
    Michael Nonet
    Article
    Nonet et al bioRxiv 2024.03.01.583017
    Type
    Empty backbone
    Use
    Recombination mediated cassette exchange; recombi…
    Expression
    Worm
    Available Since
    Sept. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    244896-plasmid-map-sequence-id-485985

    Enlarge

  13. pHygGP16

    Plasmid
    #244903
    Purpose
    hygR selection nls-GFP marked rapid RMCE integration vector with an attP site for RMHE
    Depositor
    Michael Nonet
    Article
    Nonet et al bioRxiv 2024.03.01.583017
    Type
    Empty backbone
    Use
    Recombination mediated cassette exchange; recombi…
    Expression
    Worm
    Available Since
    Sept. 8, 2025
    Availability
    Academic Institutions and Nonprofits only
    244903-plasmid-map-sequence-id-485493

    Enlarge

  14. pHygRP12

    Plasmid
    #244905
    Purpose
    hygR selection nls-Scarlet marked rapid RMCE integration vector with an attP site for RMHE
    Depositor
    Michael Nonet
    Article
    Nonet et al bioRxiv 2024.03.01.583017
    Type
    Empty backbone
    Use
    Recombination mediated cassette exchange; recombi…
    Expression
    Worm
    Available Since
    Sept. 8, 2025
    Availability
    Academic Institutions and Nonprofits only
    244905-plasmid-map-sequence-id-485485

    Enlarge

  20. pCS2+ oDam_f_GFPoNLS

    Plasmid
    #85819
    Purpose
    iDamID plasmid. To express transiently the optimized E. coli Dam adenine methyltransferase fused to the nuclear-localized mmGFP via flexylinker.
    Depositor
    Joachim Wittbrodt
    Article
    Gutierrez-Triana et al Development. 2016 Oct 5. pii: dev.139261
    Insert
    oDam_f_GFPoNLS
    Tags
    oNLS (optimized nuclear localization signal) C te…
    Expression
    Bacterial, Insect, Mammalia…
    Mutation
    L122A. Codon optimization compatible to work in M…
    Available Since
    Jan. 25, 2017
    Availability
    Academic Institutions and Nonprofits only
    85819-plasmid-map-sequence-id-170478

    Enlarge

Showing: 3001 - 3020 of 6447 results