We narrowed to 8,120 results for: sars

1. CoV2-N-S235F

   Plasmid

   #177956

   Purpose

   Transient mammalian expression of codon optimized SARS-CoV-2 Nucleocapsid (S235F). Used for generating RNA packaging virus-like particles.

   Depositor

   Jennifer Doudna

   Article

   Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

   Insert

   SARS-CoV-2 Nucleocapsid (S235F) (N )
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   S235F

   Promoter

   Available since

   Nov. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. NSP3C-FR-2Strep

   Plasmid

   #210312

   Purpose

   Expresses the C terminal of SARS-CoV-2 NSP3 in mammalian cells

   Depositor

   Michele Pagano

   Article

   Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

   Insert

   C terminal of SARS-CoV-2 NSP3 (ORF1ab )
   

   Use

   Tags

   2Strep

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. NSP3C-EGFP

   Plasmid

   #210318

   Purpose

   Expresses the C terminal of SARS-CoV-2 NSP3 fused with EGFP tag in mammalian cells

   Depositor

   Michele Pagano

   Article

   Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

   Insert

   C terminal of SARS-CoV-2 NSP3 (ORF1ab )
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCEP4-myc-S (Wuhan variant)

   Plasmid

   #169846

   Purpose

   Mammalian expression plasmid for S of SARS-CoV-2 (Wuhan variant) with N-terminal c-myc tag

   Depositor

   Erik Procko

   Article

   Zhang et al Nat Chem Biol. 2022 Mar;18(3):342-351. d

   Insert

   Protein S
   

   Use

   Tags

   HA leader and c-myc tag

   Expression

   Mammalian

   Mutation

   Codon-optimized for human cell expression.

   Promoter

   CMV

   Available since

   Jan. 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.3_CoV2_D18

   Plasmid

   #170442

   Purpose

   Express SARS-CoV-2 WT spike protein with an 18aa deletion on the C-terminal tail. Used for SARS2 pseudotyped virus production.

   Depositor

   David Nemazee

   Article

   Rogers et al Science. 2020 Aug 21;369(6506):956-963.

   Insert

   SARS-CoV-2 spike D18 (S SARS-CoV-2, USA-WA1/2020 strain)
   

   Use

   Tags

   NA

   Expression

   Mammalian

   Mutation

   18aa deletion in c-terminal tail

   Promoter

   CMV

   Available since

   July 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCDNA3.1-spikeRBD::∆ACE2 SURF

   Plasmid

   #206957

   Purpose

   Fluorescent reporter of the interaction between SARS-CoV-2 spike and human ACE2

   Depositor

   Xiaokun Shu

   Article

   Yang et al Nat Microbiol. 2023 Jan;8(1):121-134. do

   Inserts

   sars GU280_gp02, spike glycoprotein (S SARS-CoV-2)
   ACE2 (ACE2 Human)
   

   Use

   Tags

   cSURF, mCherry, and nSURF

   Expression

   Mammalian

   Mutation

   17-615 and RBD (319-541)

   Promoter

   CMV

   Available since

   Sept. 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pExp-His-zBasic-RBD

   Plasmid

   #194999

   Purpose

   Production of SARS-CoV2 receptor binding domain in E. coli

   Depositor

   Marko Hyvönen

   Article

   Gaynor et al Nat Commun. 2023 Jun 16;14(1):3583. doi:

   Insert

   RBD (S SARS-CoV-2)
   

   Use

   Tags

   His8-Zbasic

   Expression

   Bacterial

   Mutation

   Promoter

   T7lac

   Available since

   Jan. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCAGGS-S-B.1.1.529-FLAG

   Plasmid

   #180774

   Purpose

   B.1.1.529 variant S-protein with c-terminal FLAG tag

   Depositor

   Daniel Conway

   Insert

   S-protein B.1.1.529
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 23, 2022

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

9. pCDNA4 CoV-2 nsp1 3xFLAG

   Plasmid

   #176057

   Purpose

   Expressing WT CoV2 nsp1 in mammalian cells. This contains a 3XFLAG tag fusion to nsp1's C-terminus.

   Depositor

   Britt Glaunsinger

   Article

   Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

   Insert

   CoV-2 nsp1 (ORF1ab SARS-CoV-2)
   

   Use

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. CoV2-M-IRES-E

    Plasmid

    #177938

    Purpose

    Transient mammalian expression of codon optimized SARS-CoV-2 Membrane and Envelope proteins (original Wuhan Hu1 sequence). Used for generating RNA packaging virus-like particles.

    Depositor

    Jennifer Doudna

    Article

    Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

    Insert

    SARS-CoV-2 Membrane and Envelope proteins (original Wuhan Hu1 sequence) (E, M )
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. Luc-T20

    Plasmid

    #177941

    Purpose

    Transient mammalian expression of Luc2 (firefly luciferase) along with SARS-CoV-2 sequence 20080-22222 (T20) within the 3'UTR. Resulting transcript is packaged into SARS-CoV-2 virus-like particles.

    Depositor

    Jennifer Doudna

    Article

    Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

    Insert

    Luc2 (ORF1ab )
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. Nsp3 -EGFP

    Plasmid

    #165108

    Purpose

    mammalian expression and localization

    Depositor

    Bruno Antonny

    Article

    Miserey-Lenkei et al Biol Cell. 2021 Jul;113(7):311-328. doi:

    Insert

    Nsp3 (ORF1ab SARS-COV-2)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. Luc-PS9

    Plasmid

    #177942

    Purpose

    Transient mammalian expression of Luc2 (firefly luciferase) along with SARS-CoV-2 sequence 20080-21171 (PS9) within the 3'UTR. Resulting transcript is packaged into SARS-CoV-2 virus-like particles.

    Depositor

    Jennifer Doudna

    Article

    Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

    Insert

    Luc2 (ORF1ab )
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. CoV2-N-WT-Hu1

    Plasmid

    #177937

    Purpose

    Transient mammalian expression of codon optimized SARS-CoV-2 Nucleocapsid (original Wuhan Hu1 sequence). Used for generating RNA packaging virus-like particles.

    Depositor

    Jennifer Doudna

    Article

    Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

    Insert

    SARS-CoV-2 Nucleocapsid (original Wuhan Hu1 sequence) (N )
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCDFDuet-nsp7-nsp8

    Plasmid

    #159092

    Purpose

    Coexpression construct of Nsp7 with an N-terminal His-tag and Nsp8

    Depositor

    Seth Darst

    Article

    Chen et al Cell. 2020 Sep 17;182(6):1560-1573.e13.

    Inserts

    NSP7
    NSP8
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. CoV2-N-B.1.1.7

    Plasmid

    #177958

    Purpose

    Transient mammalian expression of codon optimized SARS-CoV-2 Nucleocapsid (D3L, R203K, G204R, S235F). Used for generating RNA packaging virus-like particles.

    Depositor

    Jennifer Doudna

    Article

    Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

    Insert

    SARS-CoV-2 Nucleocapsid (D3L, R203K, G204R, S235F) (N )
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    D3L,R203K,G204R,S235F

    Promoter

    Available since

    Nov. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET30a-Spike-E

    Plasmid

    #165096

    Purpose

    protein expression in E. coli

    Depositor

    Rick Tarleton

    Article

    Shen et al medRxiv [Preprint]. 2021 Jan 27:2021.01.

    Insert

    Severe acute respiratory syndrome coronavirus 2 spike protein (S SARS-CoV2)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 11, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

18. CoV2-N-P.1

    Plasmid

    #177959

    Purpose

    Transient mammalian expression of codon optimized SARS-CoV-2 Nucleocapsid (P80R, R203K, G204R). Used for generating RNA packaging virus-like particles.

    Depositor

    Jennifer Doudna

    Article

    Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

    Insert

    SARS-CoV-2 Nucleocapsid (P80R, R203K, G204R) (N )
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    P80R,R203K,G204R

    Promoter

    Available since

    Nov. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. SpyTag-RBD

    Plasmid

    #159999

    Purpose

    Expresses SARS-CoV-2 RBD (340-538) (receptor-binding domain from Spike) with N-terminal SpyTag fusion in mammalian cells

    Depositor

    Mark Howarth

    Article

    Rahikainen et al Angew Chem Int Ed Engl. 2020 Sep 4. doi:

    Insert

    SpyTag-SARS-CoV-2 RBD (340-538) (S SARS-CoV-2)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET-His6-SUMO-MproC145A

    Plasmid

    #172653

    Purpose

    Bacterial expression of SARS-CoV-2 Mpro C145A inactive mutant fused to SUMO

    Depositor

    Stephen Harrison

    Article

    MacDonald et al ACS Infect Dis. 2021 Sep 10;7(9):2591-25

    Insert

    SARS-CoV-2 Mpro (ORF1ab Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    6xHis Tag and SUMO

    Expression

    Bacterial

    Mutation

    Catalytic Cysteine 145 mutated to Alanine

    Promoter

    T7

    Available since

    Aug. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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