We narrowed to 9,424 results for: try

1. pmalc4x_K398R_TEV

   Plasmid

   #47701

   Purpose

   Expresses MBP myocilin olfactomedin domain fusion protein with a TEV protease recognition sequence and the mutation K398R

   Depositor

   Raquel Lieberman

   Article

   Burns et al Biochemistry. 2011 Jul 5;50(26):5824-33.

   Insert

   Olfactomedin-like domain with K398R mutation (MYOC Human)
   

   Use

   Tags

   MBP

   Expression

   Bacterial

   Mutation

   changed Lysine 398 to Arginine

   Promoter

   Ptac

   Available since

   Sept. 9, 2013

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. pCAS

   Plasmid

   #206994

   Purpose

   Plasmid expressing both SpCas9 and SaCas9

   Depositor

   Alejandro Chavez

   Article

   Kim et al Sci Adv. 2024 May 3;10(18):eadg8771. doi

   Insert

   SpCas9 - P2A - SaCas9 (NEWENTRY, NEWENTRY )
   

   Use

   Tags

   P2A

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. RRL.sin.cPPT.SFFV/Flag-Firefly luciferase.IRES-puro.WPRE (MT05)

   Plasmid

   #139446

   Purpose

   Lentiviral vector genome to ectopically express Firefly luciferase

   Depositor

   Caroline Goujon

   Article

   Doyle et al Nat Microbiol. 2018 Dec;3(12):1369-1376.

   Insert

   FLAG-tag Firefly luciferase
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   SFFV

   Available since

   Aug. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pATZ-Myc

   Plasmid

   #177718

   Purpose

   ER protein alpha-1-antitrypsin containing E342K mutation (ATZ mutant), myc tagged

   Depositor

   Erik Böttger

   Article

   Cortes Sanchon et al Commun Biol. 2021 Dec 2;4(1):1350. doi:

   Insert

   alpha-1-antitrypsin (SERPINA1 Human)
   

   Use

   Tags

   Myc

   Expression

   Bacterial and Mammalian

   Mutation

   E342K (ATZ mutant)

   Promoter

   Available since

   Jan. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. anti-FLAG M2 heavy chain

   Plasmid

   #175359

   Purpose

   Mammalian expression vector for over-expression of anti-FLAG M2 heavy chain (C-terminal His8)

   Depositor

   Joost Snijder

   Article

   Peng et al J Proteome Res. 2021 Jul 2;20(7):3559-35

   Insert

   anti-FLAG M2 heavy chain
   

   Use

   Tags

   His8 tag and N-terminal secretion signal peptide …

   Expression

   Mammalian

   Mutation

   L51I (see paper)

   Promoter

   CMV

   Available since

   Oct. 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pTmpl-miniDonor-GFP

   Plasmid

   #238107

   Purpose

   Mini donor expressing GFP, used for PCR to generate IVT template.

   Depositor

   Feng Zhang

   Article

   Edmonds et al Nat Commun. 2025 Jul 2;16(1):6079. doi:

   Insert

   CMV-GFP-syntheticPA flanked by R2Tg UTRs and 28S sequence
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLenti-Rac1-2G

   Plasmid

   #66111

   Purpose

   Second generation Rac1 FRET biosensor for lentivirus production

   Depositor

   Olivier Pertz

   Article

   Fritz et al Dev Cell. 2015 Sep 29. pii: S1534-5807(1

   Insert

   Second generation Rac1 FRET biosensor
   

   Use

   Lentiviral

   Tags

   His-Myc

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 1, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. plentiCMV-sct-p5-b2m-HLA-A0101

   Plasmid

   #196512

   Purpose

   Express single-chain peptide-MHC (CMV p5 epitope on HLA-A0101)

   Depositor

   Howard Chang

   Article

   Yu et al Cell. 2022 Dec 22;185(26):4904-4920.e22.

   Insert

   single chain of a CMV peptide, b2m and HLA A0101
   

   Use

   Lentiviral

   Tags

   NA

   Expression

   Mammalian

   Mutation

   Y84C

   Promoter

   CMV

   Available since

   March 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. ND4.2-Left TALE-G1397-N-DddA11-mCherry

   Plasmid

   #179682

   Purpose

   Expression of base editor in mammalian cells and for cell sorting

   Depositor

   David Liu

   Article

   Mok et al Nat Biotechnol. 2022 Sep;40(9):1378-1387

   Insert

   COX8a MTS_3xFLAG_ND4.2 Left TALE_2aa_DddA G1397-N (S1330I A1341V N1342S E1370K T1380I)_1xUGI_P2A_mCherry_ATP5B 3'UTR
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   S1330I A1341V N1342S E1370K T1380I

   Promoter

   Available since

   April 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pACE-GIRK2-S

    Plasmid

    #172426

    Purpose

    Expression of full-length, human GIRK2 with C-terminal StrepTag II

    Depositor

    Arthur Laganowsky

    Article

    Qiao et al Biochemistry. 2021 Dec 21;60(50):3813-38

    Insert

    GIRK2 (KCNJ6 Human)
    

    Use

    Tags

    Streptag II

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Oct. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pENTR_L1-YFP-Turbo-L2

    Plasmid

    #127350

    Purpose

    gateway entry vector for making N-terminal YFP-TurboID fusions with nuclear and non-nuclear proteins

    Depositor

    Dominique Bergmann

    Article

    Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1

    Insert

    TurboID (BirA mutant)
    

    Use

    Gateway entry vector

    Tags

    GS linker, V5, and YFP

    Expression

    Mutation

    Q65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…

    Promoter

    no promoter

    Available since

    Sept. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. ND4-DdCBE- left side TALE

    Plasmid

    #157844

    Purpose

    expresses left-side TALE–DddAtox half ND4-DdCBE in mammalian cells

    Depositor

    David Liu

    Article

    Mok et al Nature. 2020 Jul 8. pii: 10.1038/s41586-

    Insert

    SOD2 MTS–3x-HA–ND4 left tale–G1397 DddA-C–UGI–SOD2 3'UTR
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. ND4-DdCBE-right side TALE

    Plasmid

    #157843

    Purpose

    expresses right-side TALE–DddAtox half ND4-DdCBE in mammalian cells

    Depositor

    David Liu

    Article

    Mok et al Nature. 2020 Jul 8. pii: 10.1038/s41586-

    Insert

    COX8A MTS–3xFLAG–ND4 right TALE–G1397 DddA-N–UGI–ATP5B 3'UTR
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET21-pelB-LaG94-5

    Plasmid

    #228965

    Purpose

    For bacterial expression of anti-GFP nanobody LaG94-5, with pelB leader for periplasmic secretion, and C-term free cysteine and 6xHIS tag.

    Depositor

    Michael Rout

    Article

    Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

    Insert

    LaG94-5
    

    Use

    Tags

    6xHIS

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Jan. 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. opto-Cdc42

    Plasmid

    #174510

    Purpose

    Expresses opto-Cdc42 (mCherry-GGGSx2-Cdc42-GGGSx2-BcLOV4-GGGS-3xFLAG) in a pcDNA3.1 backbone.

    Depositor

    Brian Chow

    Article

    Berlew et al ACS Synth Biol. 2022 Jan 21;11(1):515-52

    Insert

    opto-Cdc42-mCherry
    

    Use

    Tags

    3xFLAG and mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pENTR_L1-miniTurbo-YFP-NLS-STOP-L2

    Plasmid

    #127362

    Purpose

    gateway entry vector for expressing nuclear miniTurbo-YFP under a promoter of choice

    Depositor

    Dominique Bergmann

    Article

    Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1

    Insert

    miniTurbo (BirA mutant)
    

    Use

    Gateway entry vector

    Tags

    GS linker, NLS, V5, and YFP

    Expression

    Mutation

    aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …

    Promoter

    no promoter

    Available since

    Sept. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pRK172-6xHis-0N4R-P301S-tau-HiBiT

    Plasmid

    #187835

    Purpose

    Expression of 6xHis-tagged P301S tau of 0N4R isoform with a C-terminal HiBiT tag for bimolecular luminescence with LgBiT.

    Depositor

    William McEwan

    Article

    Tuck et al Cell Rep. 2022 May 3;39(5):110776. doi:

    Insert

    6XHis-0N4R-P301S-tau-HiBiT
    

    Use

    Tags

    6xHistidine and HiBiT

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET21-pelB-LaG94-15

    Plasmid

    #228971

    Purpose

    For bacterial expression of anti-GFP nanobody LaG94-15, with pelB leader for periplasmic secretion, and C-term free cysteine and 6xHIS tag.

    Depositor

    Michael Rout

    Article

    Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

    Insert

    LaG94-15
    

    Use

    Tags

    6xHIS

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Jan. 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET21-pelB-LaMIgG-14

    Plasmid

    #228994

    Purpose

    For bacterial expression of anti-mouse IgG nanobody LaMIgG-14, with pelB leader for periplasmic secretion, and C-term free cysteine and C-term 6xHIS tag.

    Depositor

    Michael Rout

    Article

    Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

    Insert

    LaMIgG-14
    

    Use

    Tags

    6xHIS

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Jan. 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pEVOL-AckRS

    Plasmid

    #137976

    Purpose

    sequence optimized N‐acetyl lysyl‐tRNA synthetase with cognate tRNA for genetic code expansion

    Depositor

    Wenshe Liu

    Article

    Wang et al J Am Chem Soc. 2019 Feb 13;141(6):2462-2

    Insert

    AckRS and pylTcua
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    araBAD

    Available since

    Feb. 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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