We narrowed to 9,520 results for: try

1. pENTR_Trf2

   Plasmid

   #125159

   Purpose

   Gateway entry clone

   Depositor

   Alexander Stark, Julius Brennecke

   Article

   Haberle et al Nature. 2019 Jun;570(7759):122-126. doi:

   Insert

   Trf2 (Trf2 Fly)
   

   Use

   Gateway entry vector

   Available Since

   May 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pJB03

   Plasmid

   #111961

   Purpose

   Expresses MBP-GFP-DHFR; construct generated for pull-down and localization experiments

   Depositor

   Matthew Good

   Article

   Caldwell et al Biochemistry. 2018 May 8;57(18):2590-259

   Inserts

   mEGFP
   eDHFR
   

   Tags

   DHFR

   Expression

   Bacterial

   Mutation

   eDHFR;residues 2-159 and enhanced GFP with monome…

   Promoter

   Tac

   Available Since

   Aug. 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBad-CA TAG186

   Plasmid

   #105837

   Purpose

   Expresses thermostable variant of human carbonic anhydrase II with TAG stop codon at site 186 and C-terminal 6x his tag

   Depositor

   Ryan Mehl

   Article

   Mehl et al 2020

   Insert

   human carbonic anhydrase II thermostable variant (CA2 Human)
   

   Tags

   6x his tag

   Expression

   Bacterial

   Mutation

   TAG stop codon at site 186

   Promoter

   pBad/arabinose

   Available Since

   March 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBad-CAts TAG97

   Plasmid

   #105668

   Purpose

   expresses thermostable variant of human carbonic anhydrase II with TAG stop codon at site 97 and C-terminal 6x his tag

   Depositor

   Ryan Mehl

   Article

   Mehl et al 2020

   Insert

   human carbonic anhydrase II thermostable variant (CA2 Human)
   

   Tags

   6x his tag

   Expression

   Bacterial

   Mutation

   TAG stop codon at site 97

   Promoter

   pBad/arabinose

   Available Since

   March 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3-HA-REDD1 S137D (#628)

   Plasmid

   #99944

   Purpose

   mammalian expression

   Depositor

   James Brugarolas

   Article

   Vega-Rubin-de-Celis et al Biochemistry. 2010 Mar 23;49(11):2491-50

   Insert

   REDD1 (DDIT4 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   S137D

   Available Since

   Nov. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. RNAPII_CTD27-52_N2A

   Plasmid

   #98677

   Purpose

   Expresses 6xHis-tagged C-terminal domain of RNA polymerase II with N2A

   Depositor

   Nicolas Fawzi

   Article

   Janke et al Biochemistry. 2017 Oct 11. doi: 10.1021/

   Insert

   RNA polymerase II C-terminal domain (POLR2A Human)
   

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   N1775A, N1782A

   Available Since

   Nov. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. RNAPII_CTD27-52_del1961-1970

   Plasmid

   #98680

   Purpose

   Expresses 6xHis-tagged C-terminal domain of RNA polymerase II with del1961-1970

   Depositor

   Nicolas Fawzi

   Article

   Janke et al Biochemistry. 2017 Oct 11. doi: 10.1021/

   Insert

   RNA polymerase II C-terminal domain (POLR2A Human)
   

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   del1961-1970

   Available Since

   Nov. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. RNAPII_CTD27-52_N2A_S1966C

   Plasmid

   #98682

   Purpose

   Expresses 6xHis-tagged C-terminal domain of RNA polymerase II with N2A and S1966C

   Depositor

   Nicolas Fawzi

   Article

   Janke et al Biochemistry. 2017 Oct 11. doi: 10.1021/

   Insert

   RNA polymerase II C-terminal domain (POLR2A Human)
   

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   N1775A, N1782A, S1966C

   Available Since

   Nov. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. RNAPII_CTD27-52_K5S_S1966C

   Plasmid

   #98683

   Purpose

   Expresses 6xHis-tagged C-terminal domain of RNA polymerase II with K5S and S1966C

   Depositor

   Nicolas Fawzi

   Article

   Janke et al Biochemistry. 2017 Oct 11. doi: 10.1021/

   Insert

   RNA polymerase II C-terminal domain (POLR2A Human)
   

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   K1838S, K1859S, K1866S, K1873S, K1887S, S1966C

   Available Since

   Nov. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3-HA-REDD1 S103W (#623)

    Plasmid

    #99941

    Purpose

    mammalian expression

    Depositor

    James Brugarolas

    Article

    Vega-Rubin-de-Celis et al Biochemistry. 2010 Mar 23;49(11):2491-50

    Insert

    REDD1 (DDIT4 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    S103W

    Available Since

    Nov. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pENTR221-WNT1

    Plasmid

    #79499

    Purpose

    MultiSite Gateway entry clones, second fragment (attL5, attL2) Human WNT1 CDS

    Depositor

    Peter Jon Nelson

    Article

    Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

    Insert

    WNT1 (WNT1 Human)
    

    Use

    Multisite gateway entry clone

    Promoter

    no Promoter

    Available Since

    May 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pJK381

    Plasmid

    #71573

    Purpose

    Produces Thermoplasma acidophilum citrate synthase, Asp317>Gly mutant (TpCS-D317G)

    Depositor

    T. J. Kappock

    Article

    Kurz et al Biochemistry. 2009 Aug 25;48(33):7878-91

    Insert

    citrate synthase
    

    Expression

    Bacterial

    Mutation

    changes aspartate-317 to glycine

    Promoter

    recA

    Available Since

    April 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pJK382

    Plasmid

    #71574

    Purpose

    Produces Thermoplasma acidophilum citrate synthase, Asp317>Asn mutant (TpCS-D317N)

    Depositor

    T. J. Kappock

    Article

    Kurz et al Biochemistry. 2009 Aug 25;48(33):7878-91

    Insert

    citrate synthase
    

    Expression

    Bacterial

    Mutation

    changes aspartate-317 to asparagine; silent mutat…

    Promoter

    recA

    Available Since

    March 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pJK347

    Plasmid

    #71833

    Purpose

    Cloned region contains Acetobacter aceti 1023 hypothetical protein AZ09_02685, purE(H59D), and purK genes

    Depositor

    T. J. Kappock

    Article

    Constantine et al Biochemistry. 2006 Jul 11;45(27):8193-20

    Inserts

    hypothetical protein
    N5-carboxyaminoimidazole ribonucleotide mutase
    N5-carboxyaminoimidazole ribonucleotide synthetase
    

    Expression

    Bacterial

    Mutation

    changes histidine-59 to aspartate

    Available Since

    Jan. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pJK348

    Plasmid

    #71832

    Purpose

    Cloned region contains Acetobacter aceti 1023 hypothetical protein AZ09_02685, purE(H59N), and purK genes

    Depositor

    T. J. Kappock

    Article

    Constantine et al Biochemistry. 2006 Jul 11;45(27):8193-20

    Inserts

    hypothetical protein
    N5-carboxyaminoimidazole ribonucleotide mutase
    N5-carboxyaminoimidazole ribonucleotide synthetase
    

    Expression

    Bacterial

    Mutation

    changes histidine-59 to asparagine

    Available Since

    Jan. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pJK193

    Plasmid

    #71834

    Purpose

    Cloned region contains Acetobacter aceti 1023 rpmE-radC(and beyond) region

    Depositor

    T. J. Kappock

    Article

    Constantine et al Biochemistry. 2006 Jul 11;45(27):8193-20

    Inserts

    N5-carboxyaminoimidazole ribonucleotide mutase
    N5-carboxyaminoimidazole ribonucleotide synthetase
    

    Use

    Phage lambda cloning vector with genomic dna inse…

    Mutation

    additional N-terminal methionine encoded by this …

    Available Since

    Jan. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pFOSGE_COF4F04

    Plasmid

    #71030

    Purpose

    Gateway entry clone

    Depositor

    Alexander Stark

    Article

    Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

    Insert

    Sin3A (Sin3A Fly)
    

    Use

    Gateway entry vector

    Available Since

    Dec. 1, 2015

    Availability

    Academic Institutions and Nonprofits only

18. pFOSGE_COF1B10

    Plasmid

    #70840

    Purpose

    Gateway entry clone

    Depositor

    Alexander Stark

    Article

    Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

    Insert

    Sce (Sce Fly)
    

    Use

    Gateway entry vector

    Available Since

    Nov. 24, 2015

    Availability

    Academic Institutions and Nonprofits only

19. pFOSGE_COF2C08

    Plasmid

    #70911

    Purpose

    Gateway entry clone

    Depositor

    Alexander Stark

    Article

    Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

    Insert

    Taf5 (Taf5 Fly)
    

    Use

    Gateway entry vector

    Available Since

    Nov. 19, 2015

    Availability

    Academic Institutions and Nonprofits only

20. pFOSGE_COF2C05

    Plasmid

    #70891

    Purpose

    Gateway entry clone

    Depositor

    Alexander Stark

    Article

    Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

    Insert

    Orc2 (Orc2 Fly)
    

    Use

    Gateway entry vector

    Available Since

    Nov. 18, 2015

    Availability

    Academic Institutions and Nonprofits only