We narrowed to 8,806 results for: CAG

1. pKDsgRNA-lpoB

   Plasmid

   #89959

   Purpose

   Contains arabinose-induced lambda Red and a tet-inducible gRNA targeting lpoB.

   Depositor

   Michael Lynch

   Article

   Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

   Insert

   lpoB gRNA
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pTet

   Available since

   June 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pNDGG022

   Plasmid

   #231306

   Purpose

   Vector part for BEVA; BEVA2.0 Position 1 Level 1 BsaI cloning site with alternate CAGA 3' fusion site, without T0, AmpR

   Depositor

   Barney Geddes

   Article

   Geddes et al Can J Microbiol. 2025 Jan 1;71:1-13. doi

   Insert

   BEVA2.0 Position 1 Level 1 BsaI cloning site with alternate CAGA 3' fusion site, without T0, AmpR
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 27, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAPM-miR30-MYD88-ts2

   Plasmid

   #174281

   Purpose

   MYD88 knockdown

   Depositor

   Jeremy Luban

   Article

   Guney et al Proc Natl Acad Sci U S A. 2024 Jul 16;12

   Insert

   MYD88 shRNA (MYD88 Human)
   

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mutation

   Promoter

   SFFV

   Available since

   Aug. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRDB_162

   Plasmid

   #216092

   Purpose

   Cas9 [Sp] CRISPRi targeting CD47, positive control

   Depositor

   John Doench

   Article

   McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

   Insert

   CD47 guide
   

   Use

   CRISPR and Lentiviral; Positive controls

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRDA_832

   Plasmid

   #216085

   Purpose

   Cas9 [Sp] knockout targeting CD47, positive control

   Depositor

   John Doench

   Article

   McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

   Insert

   CD47 guide
   

   Use

   CRISPR and Lentiviral; Positive controls

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. TDP-43 mCerulean

   Plasmid

   #216227

   Purpose

   TDP-43 with a C-terminal mCerulean tag for expression in mammalian cells.

   Depositor

   Jennifer Lee

   Article

   Shadish et al Biophys Chem. 2024 Jan 23;307:107191. do

   Insert

   TDP-43 (TARDBP Human)
   

   Use

   Tags

   mCerulean

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCF821-sgCIDE-21_U6-sgRNA-EF1a-mNeonGreen

   Plasmid

   #211671

   Purpose

   U6-sgRNA-EF1a-mNeonGreen

   Depositor

   Christof Fellmann

   Article

   Tan et al Cell Rep. 2023 Nov 28;42(11):113339. doi

   Insert

   SpyCas9 and sgCIDE-21 guide RNA vector
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. 1114H

   Plasmid

   #200640

   Purpose

   An. gambiae transgenesis plasmid. Expresses gRNAs targeting B2-tubulin, ZPG, and DSXF. Crossing to Cas9 generates sterile males

   Depositor

   Omar Akbari

   Article

   Smidler et al bioRxiv [Preprint]. 2023 Jul 21:2023.07.

   Insert

   gRNA targeting DSXF, ZPG, and B2-tubulin. Actin5c-CFP and Vasa2-EYFP reporters.
   

   Use

   CRISPR

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Dec. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSC-cUnaG-LPETG

   Plasmid

   #207635

   Purpose

   A plasmid encoding photocaged SpyCatcher (pSC) fused to C-terminal UnaG fragment and LPETG sortase recognition sequence.

   Depositor

   Cole DeForest

   Article

   Ruskowitz et al Nat Chem. 2023 May;15(5):694-704. doi: 1

   Insert

   photocaged SpyCatcher-GGSGGGSG-cUnaG-LPETG
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Amber stop codon at SC's critical lysine

   Promoter

   T7

   Available since

   Oct. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMOD_B-Pho2-1/2-2-tRNA

    Plasmid

    #161761

    Purpose

    MODULE B tRNA vector with 6x gRNAs targeting the four MsPho2-1abcd and MsPho2-2abcd genes in alfalfa (pMOD_B2303 - #91068)

    Depositor

    Shaun Curtin

    Article

    Miller et al G3 (Bethesda). 2022 May 30;12(6). pii: 6

    Insert

    gRNAs
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    CmYLCV Promoter

    Available since

    May 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMOD_B-Pho2-1/2-2-Csy4

    Plasmid

    #161760

    Purpose

    MODULE B Csy4 vector with 6x gRNAs targeting the four MsPho2-1abcd and MsPho2-2abcd genes in alfalfa (pMOD_B2103 - #91061)

    Depositor

    Shaun Curtin

    Article

    Miller et al G3 (Bethesda). 2022 May 30;12(6). pii: 6

    Insert

    gRNAs
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    CmYLCV Promoter

    Available since

    May 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. Lenti-guide-puro mYwhae -2

    Plasmid

    #198498

    Purpose

    lentiviral stable expression of mYwhae gRNA 2

    Depositor

    Suzanne Pfeffer

    Article

    Dhekne et al Elife. 2023 Oct 24;12:e87098. doi: 10.75

    Insert

    mYwhae (Ywhae Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    April 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. Lenti-guide-puro mYwhae - 1

    Plasmid

    #198497

    Purpose

    lentiviral stable expression of mYwhae gRNA 1

    Depositor

    Suzanne Pfeffer

    Article

    Dhekne et al Elife. 2023 Oct 24;12:e87098. doi: 10.75

    Insert

    mYwhae (Ywhae Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    April 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pC1300_pUB10_pcoCASphi_E9t_V2_CmYLCVp_35sT_ribozyme_AtPDS3_gRNA10

    Plasmid

    #197958

    Purpose

    T-DNA binary vector to express pcoCasphi driven by UBQ10 gene promoter and a single AtPDS3 gRNA driven by CmYLCVp and flanked by ribozymes.

    Depositor

    Steven Jacobsen

    Article

    Li et al Proc Natl Acad Sci U S A. 2023 Jan 24;12

    Inserts

    pcoCasphi-2
    AtPDS3 gRNA10
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    CmYLCVp and pUBQ10

    Available since

    March 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. UAS-4x(tRNA::hiw{sgRNA})

    Plasmid

    #187884

    Purpose

    Gal4/UAS sgRNA expression targeting hiw

    Depositor

    Lukas Neukomm

    Article

    Llobet Rosell et al Elife. 2022 Dec 7;11:e80245. doi: 10.755

    Insert

    4 sgRNAs targeting hiw
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pW229-lenti-sg2-mmSerpinh1-pEF1s-NLS-mScarlet-I-P2A-BlastR

    Plasmid

    #189945

    Purpose

    Lentiviral vector to co-express a mouse Serpinh1 spsgRNA (sg2-Serpinh1) with NLS-mScarlet-I

    Depositor

    Kenneth Yamada

    Article

    Sekiguchi et al J Dent Res. 2023 Feb;102(2):197-206. doi

    Insert

    NLS-mScarlet-I-P2A-BlastR; Serpinh1 spsgRNA #2
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. rtTA-sg-tdTomato166/892

    Plasmid

    #188488

    Purpose

    gRNA plasmid with neo resistance expressing a double cutting single gRNA targeting tdTomato fluorescent protein sites 166 & 892.

    Depositor

    Johan Sosa, Indeever Madireddy

    Article

    Madireddy et al MicroPubl Biol. 2022 Jun 16;2022. doi: 1

    Insert

    tdTomato sgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    None

    Promoter

    U6

    Available since

    Aug. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pPBO.ACT004

    Plasmid

    #182714

    Purpose

    Constituitvely expressed CasRx crRNA cloning vector, extended 3' terminator region

    Depositor

    Joseph Schoeniger

    Article

    Otoupal et al Nucleic Acids Res. 2022 Aug 11. pii: 666

    Insert

    CasRx crRNA cloning backbone
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Catalytically deactivated R295A, H300A, R849A, H8…

    Promoter

    pJ23119 (TTGACAGCTAGCTCAGTCCTAGGTATAATACTAGT)

    Available since

    Aug. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. 1056J

    Plasmid

    #183136

    Purpose

    Plasmid supports expression of 2 gRNAs targeting D.suzukii sxl, and 1 gRNA targeting D.suzukii bTub,Opie-mVenus tagged, and can be integrated with pBac.

    Depositor

    Omar Akbari

    Article

    Kandul et al GEN Biotechnology (2022) 1:4, 372-385

    Insert

    U6.3-gRNAs[sxl, bTub]
    

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    June 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pBBK14 pCAS-Tyr-[gRNA: 4=XII-5] (SplitKanR, AmpR)

    Plasmid

    #178998

    Purpose

    Sp.Cas9 and gRNA yeast expression vector withXII-5 gRNA pre-cloned. Selection =SplitKanamycin resistance

    Depositor

    Vincent Martin

    Article

    Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

    Insert

    S. pyogenes Cas9
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pX459-sg-tdTomato166/892

    Plasmid

    #179919

    Purpose

    Double cutting Cas9 plasmid with puromycin resistance and a single guide RNA targeting tdTomato fluorescent protein sites 166 and 892.

    Depositor

    Johan Sosa, Indeever Madireddy

    Article

    Madireddy et al MicroPubl Biol. 2022 Jun 16;2022. doi: 1

    Insert

    tdTomato sgRNA
    

    Use

    CRISPR

    Tags

    2A-Puro and 3XFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    April 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pMKO.1-shSpc25

    Plasmid

    #160960

    Purpose

    Spc25 shRNA in pMKO.1 retroviral vector

    Depositor

    Matthew Steinhauser

    Article

    Zhang et al J Clin Invest. 2018 Nov 1;128(11):4898-4

    Insert

    Spc25 shRNA (Spc25 Mouse)
    

    Use

    RNAi and Retroviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    Dec. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pRS424-ysg(G:H)

    Plasmid

    #138257

    Purpose

    Expression of GFP-targeting sgRNAs to the left (G) and right (H) of iCas9 recognition sequence to be used in yeast dual reporter system

    Depositor

    Xiao Wang

    Article

    Standage-Beier et al CRISPR J. 2019 Aug;2:209-222. doi: 10.10

    Insert

    GFP targeting sgRNAs G and H
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    SNR52

    Available since

    March 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pUC19-hsg(G:H)

    Plasmid

    #138259

    Purpose

    Expression of humanized mCherry-targeting sgRNAs to the left (G) and right (H) of iCas9 recognition sequences to be used in Traffic Light reporter system

    Depositor

    Xiao Wang

    Article

    Standage-Beier et al CRISPR J. 2019 Aug;2:209-222. doi: 10.10

    Insert

    mCherry targeting sgRNAs G and H
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    March 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pDR-itga9_sgRNA4

    Plasmid

    #132980

    Purpose

    itga9 sgRNA targeting to "5'-TCCGCTGCCAGCCAGCCGG-3" in pDR274

    Depositor

    Nathan Lawson

    Article

    Shin et al Dev Cell. 2019 Nov 4;51(3):374-386.e5. d

    Insert

    itga9 sgRNA4 target sequence
    

    Use

    CRISPR; Sgrna generation vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pU6-sgRNA EF1Alpha-puro-T2A-BFP.PAPD4-2

    Plasmid

    #128757

    Purpose

    Expresses PAPD4 gRNA for CRISPRi

    Depositor

    David Bartel

    Article

    Kleaveland et al Cell. 2018 Jul 12;174(2):350-362.e17. do

    Insert

    PAPD4 sgRNA #1 (TENT2 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    mU6

    Available since

    Aug. 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pTBL601 AG

    Plasmid

    #126952

    Purpose

    To target a protospacer with AG at the cut site.

    Depositor

    Chang Liu

    Article

    Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

    Insert

    spacer against human genome with AG at cut site
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    human U6

    Available since

    May 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. gh104

    Plasmid

    #106774

    Purpose

    expression of gRNA targeting XXYLT1

    Depositor

    Eric-Paul Bennett

    Article

    Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

    Insert

    XXYLT1 (XXYLT1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. gh264

    Plasmid

    #106863

    Purpose

    expression of gRNA targeting ALG10B

    Depositor

    Eric-Paul Bennett

    Article

    Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

    Insert

    ALG10B (ALG10B Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pSLQ1371-sgYAP-10

    Plasmid

    #121424

    Purpose

    sgYAP-10 sequence: GTGCTGTCCCAGATGAACGTC. Lentiviral mouse U6 (mU6) promoter-driven expression vector that coexpessed Puro-T2A-mCherry from a CMV promoter.

    Depositor

    Ovijit Chaudhuri, Stanley Qi

    Article

    Lee et al Nat Commun. 2019 Apr 23;10(1):1848. doi:

    Insert

    sgYAP-10 (GTGCTGTCCCAGATGAACGTC)
    

    Use

    CRISPR and Lentiviral

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    mouse U6

    Available since

    April 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge