We narrowed to 23,563 results for: CHI

1. pCMV-RdLight1

   Plasmid

   #125706

   Purpose

   Expresses the red genetically encoded dopamine sensor RdLight1 in mammalian cells.

   Depositor

   Lin Tian

   Article

   Patriarchi et al Nat Methods. 2020 Nov;17(11):1147-1155.

   Insert

   RdLight1
   

   Use

   Tags

   Flag tag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. lenti-25xAARE-minP-EGFP

   Plasmid

   #159666

   Purpose

   EGFP reporter plasmid containing 25 tandem repeats of the amino acid response element (AARE)

   Depositor

   Seiichi Oyadomari

   Article

   Kitakaze et al Elife. 2019 Dec 17;8. pii: 43302. doi: 1

   Insert

   25xAARE-minP-EGFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET11a-hRanGAP1

   Plasmid

   #50941

   Purpose

   expression of full length untagged human RanGAP1 in E.coli

   Depositor

   Frauke Melchior

   Article

   Werner et al Mol Cell. 2012 May 11;46(3):287-98. doi:

   Insert

   RanGTPase activating protein 1 (RANGAP1 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   May 30, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. 3xFLAG-dCas9/pMSCVhyg

   Plasmid

   #134323

   Purpose

   Expresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to purify specific genomic regions of interest.

   Depositor

   Hodaka Fujii

   Article

   Yuno et al Biol Methods Protoc. 2021 Jul 9;6(1):bpa

   Insert

   3xFLAG-dCas9
   

   Use

   CRISPR and Retroviral

   Tags

   3xFLAG tag and NLS

   Expression

   Mammalian

   Mutation

   human codon-optimized, D10A + H840A

   Promoter

   LTR

   Available since

   April 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pKD-SepRS-EFSep

   Plasmid

   #34623

   Depositor

   Dieter Söll, Jesse Rinehart

   Article

   Park et al Science. 2011 Aug 26;333(6046):1151-4.

   Inserts

   SepRS (MMP0688 Methanocaldococcuc maripaludis)
   EF-Sep (tufB E. coli)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   His67 to Arg , Glu216 to Asn, Asp217 to Gly, Phe2…

   Promoter

   Ptac and Ptac (from SepRS- operon like)

   Available since

   Feb. 2, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET28a-His-hAos1

   Plasmid

   #53135

   Purpose

   E.coli expression of full length human Aos1 (SAE1) with Nterminal His-tag

   Depositor

   Frauke Melchior

   Article

   Pichler et al Cell. 2002 Jan 11;108(1):109-20.

   Insert

   Aos1 (SAE1 Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Sept. 17, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. XNM-Luc

   Plasmid

   #35157

   Depositor

   Linda Penn

   Article

   Facchini et al Mol Cell Biol. 1997 Jan;17(1):100-14.

   Insert

   Myc promoter (MYC Human)
   

   Use

   Luciferase

   Tags

   Luciferase

   Expression

   Mammalian

   Mutation

   Contains 152 bp of the myc promoter

   Promoter

   c-myc

   Available since

   Oct. 23, 2012

   Availability

   Academic Institutions and Nonprofits only

8. pSN837

   Plasmid

   #184825

   Purpose

   Expresses human KIF5A in mammalian cells

   Depositor

   Kyoko Chiba

   Article

   Nakano et al Genes Cells. 2022 Apr 17. doi: 10.1111/g

   Insert

   KIF5A (KIF5A Human)
   

   Use

   Tags

   mScarlet

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET32a-hTGFa

   Plasmid

   #199236

   Purpose

   Bacterial production of soluble, active target proteins; Nterm thrombin and enterokinase cleavage sites.

   Depositor

   Nilson Zanchin

   Article

   Ferreira et al Sci Rep. 2022 Mar 23;12(1):5034. doi: 10

   Insert

   Transforming growth factor alpha, partial (TGFA Human)
   

   Use

   Tags

   TrxA-6xHis-S-tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   May 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCIneoMyc rat SAPAP1

    Plasmid

    #40215

    Depositor

    Yutaka Hata, Yoshimi Takai

    Article

    Takeuchi et al J Biol Chem. 1997 May 2;272(18):11943-51

    Insert

    SAPAP1 (Dlgap1 Rat)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 28, 2012

    Availability

    Academic Institutions and Nonprofits only

11. pEGFP-N1-mCherry-ApoB-TagBFP

    Plasmid

    #129383

    Purpose

    Plasmid bearing the editing target of APOBEC1 fused between the mCherry and the Tag-BFP one. In presence of APOBEC1, the CAA codon in ApoB is edited to a Stop codon (UAA), leading to loss of Tag-BFP

    Depositor

    Silvestro Conticello

    Article

    Chieca et al Methods Mol Biol. 2021;2181:69-81. doi:

    Insert

    mCherry-ApoB-Tag-BFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV promoter

    Available since

    May 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGBW-m4046828

    Plasmid

    #145742

    Purpose

    Bacterial Expression plasmid for SARS-CoV-2 surface glycoprotein (Spike protein)

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 S (surface glycoprotein) (S Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable TEV;6xHIS

    Expression

    Bacterial

    Mutation

    Escherichia coli recode 1

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. pBa.GFP-AP1/gamma1

    Plasmid

    #218801

    Purpose

    Mammalian expression of mouse Ap1 g1

    Depositor

    Marvin Bentley

    Article

    Montgomery et al Mol Biol Cell. 2024 Mar 6:mbcE23100401.

    Insert

    Ap1 g1 (Ap1g1 Mouse)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Chicken Beta actin

    Available since

    July 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. human EB1 in PET28a for protein expression

    Plasmid

    #39297

    Depositor

    Tim Mitchison, Jennifer Tirnauer

    Article

    Tirnauer et al Mol Biol Cell. 2002 Oct . 13(10):3614-26

    Insert

    EB1 (MAPRE1 Human)
    

    Use

    Tags

    6HIS

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 30, 2012

    Availability

    Academic Institutions and Nonprofits only

15. NET29A.Hs pEGFP-N2 (1181)

    Plasmid

    #62040

    Purpose

    mammalian expression of nuclear envelope transmembrane protein

    Depositor

    Eric Schirmer

    Article

    Malik et al Cell Mol Life Sci. 2010 Apr;67(8):1353-6

    Insert

    NET29A (TMEM120A Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Splice variant

    Promoter

    CMV

    Available since

    Jan. 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGBW-m4046785

    Plasmid

    #145684

    Purpose

    Bacterial Expression plasmid for SARS-CoV-2 N (nucleocapsid)

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 N (nucleocapsid) (N Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable TEV;StrepII

    Expression

    Bacterial

    Mutation

    Escherichia coli recode 1

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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17. pCMV rat SAPAP1

    Plasmid

    #47940

    Purpose

    Expresses rat SAPAP1 in mammalian cells

    Depositor

    Yutaka Hata, Yoshimi Takai

    Article

    Takeuchi et al J Biol Chem. 1997 May 2;272(18):11943-51

    Insert

    SAPAP1 (Dlgap1 Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 9, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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18. pBS-ACTB-C200-GFPfr1

    Plasmid

    #169798

    Purpose

    THe donor vector for the ACTB gene locus.

    Depositor

    Natsuko Chiba

    Article

    Yoshino et al Sci Rep. 2019 Feb 7;9(1):1644. doi: 10.1

    Insert

    Donor sequence to ACTB gene for HR
    

    Use

    Hr donor vector for human actb gene.

    Tags

    Expression

    Mutation

    Partial sequence of GFP is inserted before the st…

    Promoter

    Available since

    Nov. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pSN838

    Plasmid

    #184826

    Purpose

    Expresses human KIF5A(∆exon27) in mammalian cells

    Depositor

    Kyoko Chiba

    Article

    Nakano et al Genes Cells. 2022 Apr 17. doi: 10.1111/g

    Insert

    KIF5A (∆exon27) (KIF5A Human)
    

    Use

    Tags

    mScarlet

    Expression

    Mammalian

    Mutation

    ∆exon27 form

    Promoter

    CMV

    Available since

    May 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pBa.GFP-KIF13B

    Plasmid

    #134626

    Purpose

    Mammalian expression of mouse KIF13B 442-1843

    Depositor

    Marvin Bentley

    Article

    Yang et al Traffic. 2019 Aug 28. doi: 10.1111/tra.1

    Insert

    KIF13B (Kif13b Mouse)
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    Chicken Beta actin

    Available since

    Oct. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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21. pCMV_nLightG

    Plasmid

    #217656

    Purpose

    Expresses norepinephrine indicator nLightG under mammalian promoter

    Depositor

    Tommaso Patriarchi

    Article

    Kagiampaki et al Nat Methods. 2023 Sep;20(9):1426-1436. d

    Insert

    nLightG
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pEGFP-C1-hFGD5

    Plasmid

    #50760

    Purpose

    expression of EGFP-tagged human FGD5

    Depositor

    Naoki Mochizuki

    Article

    Ando et al J Cell Biol. 2013 Sep 16;202(6):901-16.

    Insert

    FGD5 (FGD5 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 14, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pcDNA3.2/V5 hsa-mir-150

    Plasmid

    #26310

    Depositor

    David Bartel

    Article

    Chiang et al Genes Dev. 2010 May 15. 24(10):992-1009.

    Insert

    hsa-mir-150 (MIR150 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 29, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pATLIC

    Plasmid

    #110287

    Purpose

    A backbone vector for cell surface display in E. coli by Ligation Independent Cloning (LIC)

    Depositor

    In-Geol Choi

    Article

    Ko et al Appl Environ Microbiol. 2012 May;78(9):3

    Insert

    yfaL
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    araBAD promoter

    Available since

    April 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. PKG I alpha CAT

    Plasmid

    #16392

    Depositor

    Bernard Weinstein

    Article

    Deguchi et al Mol Cancer Ther. 2002 Aug . 1(10):803-9.

    Insert

    PKG1 alpha (PRKG1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    CAT: catalytic domain, S65D

    Promoter

    Available since

    April 14, 2009

    Availability

    Academic Institutions and Nonprofits only

26. pCMV Myc rat SAPAP4

    Plasmid

    #40218

    Depositor

    Yutaka Hata, Yoshimi Takai

    Article

    Takeuchi et al J Biol Chem. 1997 May 2;272(18):11943-51

    Insert

    SAPAP4 (Dlgap4 Rat)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    N834D

    Promoter

    CMV

    Available since

    Nov. 28, 2012

    Availability

    Academic Institutions and Nonprofits only

27. p7274 MSCV-P C-FlagHA 16 ∆DLYC

    Plasmid

    #163304

    Purpose

    Expresses HPV16 E7 ∆DLYC

    Depositor

    Elizabeth White

    Article

    Hatterschide et al Proc Natl Acad Sci U S A. 2019 Apr 2;116

    Insert

    HPV16 E7 ∆DLYC
    

    Use

    Retroviral

    Tags

    FlagHA

    Expression

    Mutation

    ∆DLYC

    Promoter

    MSCV LTR

    Available since

    Jan. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pET15b/bPanK

    Plasmid

    #73619

    Purpose

    Produces Escherichia coli pantothenate kinase, with N-terminal His6 tag (H6PanK)

    Depositor

    T. J. Kappock, Suzanne Jackowski

    Article

    Calder et al J Biol Chem. 1999 Jan 22;274(4):2014-20.

    Insert

    pantothenate kinase (coaA Escherichia coli)
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    relative to MG1655 sequence, changes glutamate-5 …

    Promoter

    T7

    Available since

    Feb. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. p8103 LentiCRISPR v2 sgNT-2

    Plasmid

    #163313

    Purpose

    Expresses Cas9 and a non-targeting control guide RNA

    Depositor

    Elizabeth White

    Article

    Hatterschide et al Proc Natl Acad Sci U S A. 2019 Apr 2;116

    Insert

    sgNT-2
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    April 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. CREB3L3

    Plasmid

    #99509

    Purpose

    to express CREB3L3 in mammalian system

    Depositor

    Dong-Yan Jin

    Article

    Chin et al Nucleic Acids Res. 2005 Mar 30;33(6):185

    Insert

    CREB3L3 (Creb3l3 Mouse)
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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