We narrowed to 4,430 results for: TIL;

1. pEF Doc2alpha D291N mutant GFP

   Plasmid

   #128821

   Purpose

   To visualize Doc2alpha D291N mutant in PC12 cells

   Depositor

   Edwin Chapman

   Article

   Courtney et al Neuron. 2018 Jun 6;98(5):977-991.e5. doi

   Insert

   Doc2alpha (Doc2a Rat)
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   D291N

   Promoter

   EF-1alpha

   Available Since

   Nov. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEF Doc2beta D303N mutant GFP

   Plasmid

   #128822

   Purpose

   To visualize Doc2beta D303N mutant in PC12 cells

   Depositor

   Edwin Chapman

   Article

   Courtney et al Neuron. 2018 Jun 6;98(5):977-991.e5. doi

   Insert

   Doc2beta (Doc2b Rat)
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   D303N

   Promoter

   EF-1alpha

   Available Since

   Nov. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMuLE ENTR CMV tdTomato R4-R3

   Plasmid

   #62151

   Purpose

   MuLE (Multiple Lentiviral Expression) Entry vector containing a CMV promoter and tdTomato module. Compatible with MultiSite Gateway cloning

   Depositor

   Ian Frew

   Article

   Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

   Insert

   tdTomato
   

   Use

   Mule gateway entry vector

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 16, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMuLE ENTR CMV Luc2 (FF-Luciferase) R4-R3

   Plasmid

   #62169

   Purpose

   MuLE (Multiple Lentiviral Expression) Entry vector containing a CMV promoter and firefly luciferase module. Compatible with MultiSite Gateway cloning

   Depositor

   Ian Frew

   Article

   Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

   Insert

   Luciferase
   

   Use

   Luciferase; Mule gateway entry vector

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMuLE ENTR CMV eGFP L1-R5

   Plasmid

   #62142

   Purpose

   MuLE (Multiple Lentiviral Expression) Entry vector containing a CMV promoter and EGFP module. Compatible with MultiSite Gateway cloning

   Depositor

   Ian Frew

   Article

   Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

   Insert

   eGFP
   

   Use

   Mule gateway entry vector

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 16, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. KZ701: pMVP (L3-L2) AID + polyA

   Plasmid

   #121791

   Purpose

   pMVP L3-L2 entry plasmid, contains AID + polyA for 3- or 4-component MultiSite Gateway Pro assembly. Allows fusion of minimal C-term Auxin Induced Degron (AID) domain (activated by auxin) to gene

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Insert

   AID + polyA
   

   Use

   Synthetic Biology; Pmvp gateway entry plasmid

   Available Since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMuLE ENTR CMV iRFP L5-L2

   Plasmid

   #62157

   Purpose

   MuLE (Multiple Lentiviral Expression) Entry vector containing a CMV promoter and iRFP module. Compatible with MultiSite Gateway cloning

   Depositor

   Ian Frew

   Article

   Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

   Insert

   iRFP
   

   Use

   Mule gateway entry vector

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 16, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. KY801: pMVP (L3-L2) P2A-eGFP + polyA

   Plasmid

   #121764

   Purpose

   pMVP L3-L2 entry plasmid, contains eGFP-P2A + polyA for 3- or 4-component MultiSite Gateway Pro assembly. Allows expression of C-term eGFP linked by P2A to gene of interest.

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Insert

   P2A-eGFP + polyA
   

   Use

   Synthetic Biology; Pmvp gateway entry plasmid

   Available Since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGEX-hXRCC1-K176R

   Plasmid

   #52275

   Purpose

   Bacterial expression of human XRCC1-K176R with N-terminal GST-tag

   Depositor

   Primo Schaer

   Article

   Weber et al PLoS One. 2014 Jul 9;9(7):e102157. doi:

   Insert

   XRCC1 (XRCC1 Human)
   

   Tags

   GST

   Expression

   Bacterial

   Mutation

   K176R, SUMO acceptor site mutant

   Promoter

   tac

   Available Since

   July 10, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMuLE ENTR CMV mCherry R4-R3

    Plasmid

    #62147

    Purpose

    MuLE (Multiple Lentiviral Expression) Entry vector containing a CMV promoter and mCherry module. Compatible with MultiSite Gateway cloning

    Depositor

    Ian Frew

    Article

    Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

    Insert

    mCherry
    

    Use

    Mule gateway entry vector

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. LA102: pMVP (L3-L2) P2A-eYFP + WPRE

    Plasmid

    #121778

    Purpose

    pMVP L3-L2 entry plasmid, contains eYFP-P2A + WPRE for 3- or 4-component MultiSite Gateway Pro assembly. Allows expression of C-term eYFP linked by P2A to gene of interest in lentivirus vectors.

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Insert

    P2A-eYFP + WPRE
    

    Use

    Synthetic Biology; Pmvp gateway entry plasmid

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pOCC177

    Plasmid

    #118886

    Purpose

    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6-MBP tag, cleavable with 3C, and C-terminal SNAP tag, cleavable with TEV

    Depositor

    Marc Bickle, PEPC MPI CBG

    Article

    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

    Insert

    NcoI-HIS6-MBP-3C-NotI-ccdB-AscI-TEV-SNAP-stop-HindIII cassette
    

    Tags

    HIS6-MBP, cleavable with 3C protease and SNAP tag…

    Expression

    Insect

    Promoter

    polH

    Available Since

    Jan. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pOCC194

    Plasmid

    #118882

    Purpose

    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with C-terminal SNAP tag, cleavable with TEV, and C-terminal HIS6 tag, cleavable with 3C

    Depositor

    Marc Bickle, PEPC MPI CBG

    Article

    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

    Insert

    NcoI-NotI-ccdB-AscI-TEV-SNAP-3C-HIS6-stop-HindIII cassette
    

    Tags

    SNAP tag, cleavable with TEV protease, HIS6, clea…

    Expression

    Insect

    Promoter

    polH

    Available Since

    Jan. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pOCC210

    Plasmid

    #118896

    Purpose

    shuttle vector for baculovirus production, using FlashBac bacmid; for rec. protein with N-terminal MBP tag, cleavable with 3C and C-terminal SNAP, cleavable with TEV and HIS6, cleavable with 3C

    Depositor

    Marc Bickle, PEPC MPI CBG

    Article

    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

    Insert

    NcoI-MBP-3C-NotI-ccdB-AscI-TEV-SNAP-3C-HIS6-stop-HindIII cassette
    

    Tags

    MBP, cleavable with 3C protease and SNAP tag, cle…

    Expression

    Insect

    Promoter

    polH

    Available Since

    Jan. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pOCC231

    Plasmid

    #118885

    Purpose

    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6-MBP tag, cleavable with 3C, and C-terminal mCitrine, cleavable with TEV

    Depositor

    Marc Bickle, PEPC MPI CBG

    Article

    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

    Insert

    NcoI-HIS6-MBP-3C-NotI-ccdB-AscI-TEV-mCitrine-stop-HindIII cassette
    

    Tags

    HIS6-MBP, cleavable with 3C protease and mCitrine…

    Expression

    Insect

    Promoter

    polH

    Available Since

    Jan. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. LA301: pMVP (L3-L2) P2A-Hygro + WPRE

    Plasmid

    #121787

    Purpose

    pMVP L3-L2 entry plasmid, contains Hygro-P2A + WPRE for 3- or 4-component MultiSite Gateway Pro assembly. Allows expression of C-term Hygro selection marker linked by P2A to gene in lentivirus vectors

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Insert

    P2A-Hygro + WPRE
    

    Use

    Synthetic Biology; Pmvp gateway entry plasmid

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. LA802: pMVP (L3-L2) AID + WPRE

    Plasmid

    #121794

    Purpose

    pMVP L3-L2 entry plasmid, contains AID + WPRE for 3- or 4-component MultiSite Gateway Pro assembly. Allows fusion of minimal C-term AID domain (activated by auxin) to gene in lentivirus vectors

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Insert

    AID + WPRE
    

    Use

    Synthetic Biology; Pmvp gateway entry plasmid

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. KO801: pMVP (L5-L4) Hygro-P2A

    Plasmid

    #121718

    Purpose

    pMVP L5-L4 entry plasmid, contains Hygro-P2A for 4-component MultiSite Gateway Pro assembly. Allows expression of N-term Hygro selection marker linked by P2A to gene of interest.

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Insert

    Hygro-P2A (open)
    

    Use

    Synthetic Biology; Pmvp gateway entry plasmid

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCSD_SpCas9MT3-NLS-3xHA-NLS-SaCas9WT-2xNLS

    Plasmid

    #107322

    Purpose

    Expresses SpCas9 (R1335K) fused to SaCas9 in mammalian cells

    Depositor

    Scot Wolfe

    Article

    Bolukbasi et al Nat Commun. 2018 Nov 19;9(1):4856. doi:

    Insert

    SpCas9-SaCas9 fusion
    

    Use

    CRISPR

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    SpCas9 (R1335K) is fused to SaCas9

    Promoter

    CMV IE93

    Available Since

    Nov. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCSD_2XNLS-SpCas9MT3-NLS-3xHA-2XNLS-NmCas9WT-2XNLS

    Plasmid

    #107325

    Purpose

    Expresses SpCas9 (R1335K) fused to NmCas9 in mammalian cells

    Depositor

    Scot Wolfe

    Article

    Bolukbasi et al Nat Commun. 2018 Nov 19;9(1):4856. doi:

    Insert

    SpCas9-NmCas9 fusion
    

    Use

    CRISPR

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    SpCas9 (R1335K) is fused to NmCas9

    Promoter

    CMV IE94

    Available Since

    Nov. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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