We narrowed to 12,677 results for: sequence

1. pFX-mCherry-EEA1

   Plasmid

   #174452

   Purpose

   Express mCherry with early endosome-targeting sequence in mammalian cells

   Depositor

   Yusuke Ohba

   Article

   Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

   Insert

   mCherry with early endosome-targeting sequence
   

   Use

   Tags

   early endosomal antigen 1 (EEA1)

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCMV-hAR-F23A

   Plasmid

   #89089

   Purpose

   mammalian expression of human androgen receptor with mutation of N-terminal FxxLF motif

   Depositor

   Elizabeth Wilson

   Article

   He et al J Biol Chem. 2000 Jul 28;275(30):22986-9

   Insert

   hAR-FxxLF mutant (AR Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   hAR-F23A

   Promoter

   CMV

   Available since

   April 11, 2017

   Availability

   Academic Institutions and Nonprofits only

3. pU6-Sp-gRNA-CCR5_DF_B584b_attB

   Plasmid

   #182148

   Purpose

   To insert attB attachment site at CCR5 in human cells via twinPE

   Depositor

   David Liu

   Article

   Anzalone et al Nat Biotechnol. 2022 May;40(5):731-740.

   Insert

   CCR5-B584b-attB pegRNA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   April 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pU6-Sp-gRNA-CCR5_DF_A509b_attB

   Plasmid

   #182147

   Purpose

   To insert attB attachment site at CCR5 in human cells via twinPE

   Depositor

   David Liu

   Article

   Anzalone et al Nat Biotechnol. 2022 May;40(5):731-740.

   Insert

   CCR5-A509b-attB pegRNA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   March 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pUC57-mitoLbNOX

   Plasmid

   #74448

   Purpose

   This plasmid contains human codon optimized sequence of mitoLbNOX which is a water-forming NADH oxidase that can be used to increase NAD+/NADH ratio in mammalian cells.

   Depositor

   Vamsi Mootha

   Article

   Titov et al Science. 2016 Apr 8;352(6282):231-5. doi

   Insert

   mitoLbNOX
   

   Use

   Tags

   Flag tag with linker and mitochondrial targeting …

   Expression

   Mutation

   Human codon optimized

   Promoter

   Available since

   June 3, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. VTN-bio-His

   Plasmid

   #53429

   Purpose

   Expresses full-length Vitronectin precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

   Depositor

   Gavin Wright

   Article

   Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

   Insert

   VTN (VTN Human)
   

   Use

   Tags

   His tag, enzymatic biotinylation sequence, and ra…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pgRNA-humanized

   Plasmid

   #44248

   Purpose

   Expression of customizable guide RNA (gRNA) under cotnrol of murine U6 promoter, also contains a CMV-puro-t2A-mCherry expression cassette for mammalian gene knockdown

   Depositor

   Stanley Qi

   Article

   Qi et al Cell. 2013 Feb 28;152(5):1173-83. doi: 1

   Insert

   guide RNA
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   mouse U6

   Available since

   April 8, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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8. pUbC-nls-ha-stdMCP-stdGFP

   Plasmid

   #98916

   Purpose

   synonymized tandem dimer MCP fused to synonymized tandem dimer GFP

   Depositor

   Robert Singer

   Article

   Wu et al Genes Dev. 2015 Apr 15;29(8):876-86. doi

   Insert

   stdMCP-stdGFP
   

   Use

   Lentiviral

   Tags

   GFP

   Expression

   Mutation

   Promoter

   UbC

   Available since

   Aug. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pFX-EGFP-Rab7

   Plasmid

   #174457

   Purpose

   Express EGFP with late endosome-targeting sequence in mammalian cells

   Depositor

   Yusuke Ohba

   Article

   Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

   Insert

   EGFP with late endosome-targeting sequence
   

   Use

   Tags

   small GTPase (Rab7)

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pFX-EGFP-Rab5

    Plasmid

    #174454

    Purpose

    Express EGFP with early endosome-targeting sequence in mammalian cells

    Depositor

    Yusuke Ohba

    Article

    Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

    Insert

    EGFP with early endosome-targeting sequence
    

    Use

    Tags

    small GTPase (Rab5)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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