We narrowed to 10,458 results for: T7

1. pAG247 His-iFAST

   Plasmid

   #130808

   Purpose

   Expresses His-tagged iFAST in bacteria

   Depositor

   Arnaud Gautier

   Article

   Tebo et al Biochemistry. 2018 Oct 2;57(39):5648-565

   Insert

   iFAST
   

   Use

   Tags

   His-tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Sept. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDEST17-hTRPT1-iso1

   Plasmid

   #184239

   Purpose

   For purification of N-terminally His-tagged TRPT1 from bacteria

   Depositor

   Karla Feijs

   Article

   Weixler et al Nucleic Acids Res. 2022 Sep 9;50(16):942

   Insert

   TRPT1 (TRPT1 Human)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Oct. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET28a-UBC9-K14R-A129K

   Plasmid

   #228948

   Purpose

   express a fragment of the double mutant of the human UBC9 (generated from pET28-UBC9 through mutagenesis; point mutations: K14R and A129K)

   Depositor

   Marcin Suskiewicz

   Insert

   UBC9 (UBE2I Human)
   

   Use

   Tags

   His tag and thrombine cleavage site : MGHHHHHHSSG…

   Expression

   Bacterial

   Mutation

   changed Lysine 14 to Arginine, changed Alanine 12…

   Promoter

   T7-lac

   Available since

   May 13, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET-6xHis-(pos15)GFP

   Plasmid

   #89248

   Purpose

   [Edit] Expresses GFP with +15 theoretical charge in E.Coli

   Depositor

   David Liu

   Article

   Thompson et al Chem Biol. 2012 Jul 27;19(7):831-43. doi

   Insert

   His6-(pos15)-GFP
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   April 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET51b_HCAII

   Plasmid

   #187110

   Purpose

   Expression of HCAII in bacteria

   Depositor

   Kai Johnsson

   Article

   Wang et al Angew Chem Int Ed Engl. 2020 Dec 1;59(49

   Insert

   HCAII
   

   Use

   Tags

   Hisx10 and Strep

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   July 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. 6CC8 (MBD3)

   Plasmid

   #119959

   Purpose

   Bacterial expression for structure determination

   Depositor

   Cheryl Arrowsmith

   Insert

   MBD3 (MBD3 Human)
   

   Use

   Tags

   GSTtag, 6xHis and Thrombin

   Expression

   Bacterial

   Mutation

   MBD domain (contains residues 1-71)

   Promoter

   T7

   Available since

   Jan. 8, 2019

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. V72 ELP

   Plasmid

   #68938

   Purpose

   Elastin-like Polypeptide (VPGxG) with 72 repeats containing Valine guest residue

   Depositor

   Andrew MacKay

   Article

   Dhandhukia et al Biomacromolecules. 2013 Apr 8;14(4):976-

   Insert

   V72 ELP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Oct. 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pRSFDuet1_CylLS_CylM

   Plasmid

   #208760

   Purpose

   Expresses modified cytolysin S (mCylLS) in E. coli

   Depositor

   Wilfred van der Donk

   Article

   Luo et al Anal Chem. 2024 Jan 30;96(4):1767-1773.

   Inserts

   CylLS
   CylLM
   

   Use

   Tags

   Hisx6 Tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Nov. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pT7-L2-M3T1L

   Plasmid

   #33304

   Depositor

   Terence Dermody

   Article

   Kobayashi et al Virology. 2010 Mar 15;398(2):194-200. Ep

   Inserts

   L2 (L2 Orthoreovirus T1L)
   M3 (MoV1sM3gp1 Orthoreovirus T1L)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   T7

   Available since

   Dec. 5, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pRSETB-roGFP1-R12

    Plasmid

    #83311

    Purpose

    Inducible expression of redox sensitive GFP in bacteria

    Depositor

    S. James Remington

    Article

    Cannon et al Protein Sci. 2006 Jan;15(1):45-57. Epub

    Insert

    roGFP1-R12
    

    Use

    Tags

    6xHIS

    Expression

    Bacterial

    Mutation

    C48S/Q80R/S147C/Q204C/N149K/F223R/S202K

    Promoter

    T7

    Available since

    Oct. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

11. pT7-L3-S3T1L

    Plasmid

    #33305

    Depositor

    Terence Dermody

    Article

    Kobayashi et al Virology. 2010 Mar 15;398(2):194-200. Ep

    Inserts

    L3 (MoV1sL3gp1 )
    S3 (MoV1sS3gp1 )
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    T7

    Available since

    Dec. 14, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET23a-mUbc9

    Plasmid

    #53137

    Purpose

    E.coli expression of full length untagged mouse Ubc9 (protein sequence identical to human)

    Depositor

    Frauke Melchior

    Article

    Pichler et al Cell. 2002 Jan 11;108(1):109-20.

    Insert

    Ubc9 (Ube2i Mouse)
    

    Use

    Tags

    untagged

    Expression

    Bacterial

    Mutation

    protein sequence is identical to human

    Promoter

    T7

    Available since

    Sept. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. pT7-L1-M2T1L

    Plasmid

    #33303

    Depositor

    Terence Dermody

    Article

    Kobayashi et al Virology. 2010 Mar 15;398(2):194-200. Ep

    Inserts

    L1 (L1 Orthoreovirus T1L)
    M2 (M2 Orthoreovirus T1L)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    T7

    Available since

    Dec. 5, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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14. PAS528 (TJF059)

    Plasmid

    #65602

    Purpose

    pETDuet-1 Containing FadD-His

    Depositor

    Pamela Silver

    Article

    Ford et al PeerJ. 2015 Jun 30;3:e1040. doi: 10.7717

    Insert

    FadD
    

    Use

    Tags

    6x His

    Expression

    Bacterial

    Mutation

    Promoter

    T7 lacO

    Available since

    June 2, 2015

    Availability

    Academic Institutions and Nonprofits only

15. pET21-Streptavidin-Glutamate_Tag

    Plasmid

    #46367

    Depositor

    Mark Howarth

    Article

    Fairhead et al J Mol Biol. 2013 Sep 19. pii: S0022-2836

    Insert

    Streptavidin-Glutamate_Tag
    

    Use

    Tags

    6 glutamate tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 24, 2013

    Availability

    Academic Institutions and Nonprofits only

16. pSHS212

    Plasmid

    #87346

    Purpose

    Cysteine-free (C80S/C574S) S. pyogenes Cas9 expression plasmid

    Depositor

    Jennifer Doudna

    Article

    Sternberg et al Nature. 2015 Nov 5;527(7576):110-3. doi:

    Insert

    Cas9
    

    Use

    Tags

    His-MBP

    Expression

    Bacterial

    Mutation

    C80S/C574S

    Promoter

    T7

    Available since

    March 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pPSU80

    Plasmid

    #173834

    Purpose

    Expresses 80 kD Penn State ladder protein

    Depositor

    Song Tan

    Article

    Santilli et al Sci Rep. 2021 Aug 18;11(1):16703. doi: 1

    Insert

    STRHSTPABPACIL1bRCC1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDJSVT87

    Plasmid

    #109302

    Purpose

    Express periplasmic, outer membrane, or secreted proteins in E. coli - OmpA signal and His6 tag

    Depositor

    Daniel Slade

    Article

    Casasanta et al Methods Mol Biol. 2019;1960:123-138. doi

    Type

    Empty backbone

    Use

    Tags

    OmpA 1-27, 6His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pEXP5-NT/pLas eGFP

    Plasmid

    #193630

    Purpose

    Inducible expression of eGFP by the transcription factor complex LasR-3OC12 homoserine lactone (HSL).

    Depositor

    Dora Tang

    Article

    Gonzales et al ChemSystemsChem 2023

    Insert

    eGFP
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7 RNAP promoter

    Available since

    July 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET-6xHis-(pos9)GFP

    Plasmid

    #89247

    Purpose

    Expresses GFP with +9 theoretical charge in E.Coli

    Depositor

    David Liu

    Article

    Thompson et al Chem Biol. 2012 Jul 27;19(7):831-43. doi

    Insert

    His6-(pos9)-GFP
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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