We narrowed to 6,421 results for: nls
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eSpCas9(1.1)
Plasmid#138555PurposeExpresses human codon-optimized enhanced specificity SpCas9 and blasticidin resistance: EFS promoter-eSpCas9(1.1)-NLS-FLAG-P2A-BSDDepositorInserteSpCas9(1.1)
UseCRISPR and LentiviralTagsBSD, FLAG, and NLSExpressionMammalianMutationK848A, K1003A, R1060APromoterEFSAvailable SinceJune 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
PB-CAG-dCasRx-RBM25-IRES-BleoR
Plasmid#221002PurposeStable expression of dCasRx-RBM25 when co-transfected with piggyBac transposase. Zeocin selection. CAG promoter. Use with lentiviral gRNA delivery (Addgene #138150) to stably modulate splicing.DepositorInsertdCasRx-RBM25-IRES-BleoR
UseCRISPRTagsNLS and NLS-HAExpressionMammalianPromoterCAGAvailable SinceJune 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHS_Nova
Plasmid#239831PurposeNovaIscB mammalian expressionDepositorInsertNovaIscB
TagsNucleoplasmin NLS-3xHA and SV40 NLSExpressionMammalianMutationInsertion of REC domain from NbaCas9-1 and swap o…PromoterCMVAvailable SinceJuly 21, 2025AvailabilityAcademic Institutions and Nonprofits only -
pYPQ223 (dAsCpf1-SRDX)
Plasmid#86209PurposeDeactivated AsCpf1-SRDX repressor fusion; transcriptional repressor dCpf1 Gateway entry plasmidDepositorInsertdAsCpf1
UseGateway compatible cpf1 vectorsTagsNLS and SRDX repressor and NLSExpressionPlantMutationD908AAvailable SinceApril 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
SpCas9-HF1
Plasmid#138556PurposeExpresses human codon-optimized high-fidelity SpCas9 and blasticidin resistance: EFS promoter-SpCas9-HF1-NLS-FLAG-P2A-BSDDepositorInsertSpCas9-HF1
UseCRISPR and LentiviralTagsBSD, FLAG, and NLSExpressionMammalianMutationN497A, R661A, Q695A, Q926APromoterEFSAvailable SinceJune 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
SpCas9-NRCH-ABE8e(V106W)
Plasmid#198556PurposeExpresses human codon-optimized SpCas9-NRCH-ABE8e(V106W) and blasticidin resistance: EFS promoter-SpCas9-NRCH-ABE8e(V106W)-NLS-FLAG-P2A-BSDDepositorInsertSpCas9-NRCH-ABE8e(V106W)
UseCRISPR and LentiviralTagsBSD, FLAG, and NLSExpressionMammalianPromoterEFSAvailable SinceNov. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
SpCas9-ABE8e(V106W)
Plasmid#198547PurposeExpresses human codon-optimized SpCas9-ABE8e(V106W) and blasticidin resistance: EFS promoter-SpCas9-ABE8e(V106W)-NLS-FLAG-P2A-BSDDepositorInsertSpCas9-ABE8e(V106W)
UseCRISPR and LentiviralTagsBSD, FLAG, and NLSExpressionMammalianPromoterEFSAvailable SinceNov. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMKO shRNA Bmf
Plasmid#17236DepositorAvailable SinceJan. 18, 2008AvailabilityAcademic Institutions and Nonprofits only -
Cas9-sgRNA-A+B
Plasmid#149370PurposeExpresses SpCas9 and two sgRNA targeting the lenti-CDDR reporter at two distal sitesDepositorInsertsCas9
sgRNA-A
sgRNA-B
Puromycin resistance
UseCRISPRTags3xFLAG, Nucleoplasmin NLS, and SV40 NLSExpressionMammalianPromoterCBh, PGK, and U6Available SinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMJ922
Plasmid#78312PurposeExpression of His6-MBP-tagged Cas9-NLS-EGFP protein in E. coliDepositorInsertSpCas9
TagsHA-2xNLS-EGFP-NLS and His6-MBP-TEVExpressionBacterialMutationhuman codon-optimized gene sequencePromoterT7Available SinceJune 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
dxCas(3.7)-VPR
Plasmid#108383PurposeMammalian expression vector for xCas9 3.7 with VP64-p65-Rta (VPR) fused to its C-terminusDepositorInsertdxCas(3.7)-VPR
TagsNLSExpressionMammalianMutationA262T, R324L, S409I, E480K, E543D, M694I, E1219VAvailable SinceMarch 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
SpCas9-NG-SsAPOBEC3B
Plasmid#198536PurposeExpresses human codon-optimized SpCas9-NG-SsAPOBEC3B and blasticidin resistance: EFS promoter-SpCas9-NG-SsAPOBEC3B-NLS-FLAG-P2A-BSDDepositorInsertSpCas9-NG-SsAPOBEC3B
UseCRISPR and LentiviralTagsBSD, FLAG, and NLSExpressionMammalianPromoterEFSAvailable SinceNov. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
QQR1 variant
Plasmid#138564PurposeExpresses human codon-optimized SpCas9-QQR1 and blasticidin resistance: EFS promoter-QQR1-NLS-FLAG-P2A-BSDDepositorInsertQQR1
UseCRISPR and LentiviralTagsBSD, FLAG, and NLSExpressionMammalianMutationG1218R, N1286Q, I1331F, D1332K, R1333Q, R1335Q, T…PromoterEFSAvailable SinceJune 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDRM98 mKate2 Blast
Plasmid#174720PurposeExpresses the NLS-tagged mKate2 gene, P2A and the blasticidin resistance gene.DepositorInsertsmKate2
BSD
UseLentiviralTagsP2A linker and SV40 NLS with GGS linkerExpressionMammalianPromoterMND and MND (off mKate-P2A)Available SinceNov. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
TDP43-NTD
Plasmid#241024PurposeBacterial expression of TDP-43 NTDDepositorInsertTDP43-NTD
Tags6x HisExpressionBacterialMutationchanged N81 to stop codonAvailable SinceJuly 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
pSLQ10531
Plasmid#239267PurposeExpresses NLS-dCas13b-ABI for CRISPR-TO perturbation in the nucleus of cell linesDepositorInsertNLS-dPspCas13b-EGFP-ABI
UseCRISPR and LentiviralTagsFlag tag and V5 tagExpressionMammalianMutationH133A and H1058A in PspCas13bPromoterEF1aAvailable SinceSept. 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCMV-ABE7.9
Plasmid#102918Purposeexpresses ABE7.9 in mammalian cellsDepositorInsertABE7.9
TagsNLSExpressionMammalianMutationsee manuscriptPromoterCMVAvailable SinceNov. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pHS1742
Plasmid#239838PurposeOrufIscB-KRK mammalian expressionDepositorInsertOrufIscB-KRK
TagsNucleoplasmin NLS-3xHA and SV40 NLSExpressionMammalianMutationInsertion of REC domain from NbaCas9-1 + E137K/E4…PromoterCMVAvailable SinceJuly 21, 2025AvailabilityAcademic Institutions and Nonprofits only
