We narrowed to 9,402 results for: pho;

1. pET21a Sa-iPGM-bio

   Plasmid

   #179523

   Purpose

   Expresses S. aureus iPGM containing a C-terminal biotinylation site and His tag in E. coli

   Depositor

   James Inglese

   Article

   van Neer et al ACS Chem Biol. 2022 Aug 19;17(8):2284-22

   Insert

   S. aureus iPGM bio-6His
   

   Tags

   His tag and biotinylation sequence

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Sept. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET21a Mo-iPGM-bio

   Plasmid

   #179614

   Purpose

   Expresses M. orale iPGM containing a C-terminal biotinylation site and His tag in E. coli

   Depositor

   James Inglese

   Article

   van Neer et al ACS Chem Biol. 2022 Aug 19;17(8):2284-22

   Insert

   M. orale iPGM bio-6His
   

   Tags

   His tag and biotinylation sequence

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Sept. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET21a Hp-iPGM-bio

   Plasmid

   #179615

   Purpose

   Expresses H. pylori iPGM containing a C-terminal biotinylation site and His tag in E. coli

   Depositor

   James Inglese

   Article

   van Neer et al ACS Chem Biol. 2022 Aug 19;17(8):2284-22

   Insert

   H. pylori iPGM bio-6His
   

   Tags

   His tag and biotinylation sequence

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Sept. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCSmChnERT(#99)

   Plasmid

   #184063

   Purpose

   cyclofen-inducible mCherry nuclear relocation via mammalian cell transfection or mRNA synthesis

   Depositor

   Sophie Vriz

   Article

   Sinha et al Chembiochem. 2010 Mar 22;11(5):653-63. d

   Insert

   mCherry-nls-ERT2
   

   Use

   Synthetic Biology

   Expression

   Mammalian

   Mutation

   ERT2: G400V, M543A, L544A, deleted 1-281;

   Promoter

   sCMV+SP6

   Available Since

   Aug. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pETDuet1-LanCL2-C321A/C367A-C-Ter-His

   Plasmid

   #154188

   Purpose

   To express LanCL2-C321A/C367A in bacterial cells

   Depositor

   Wilfred van der Donk

   Article

   Lai et al Cell. 2021 Apr 27. pii: S0092-8674(21)00

   Insert

   LanCL2
   

   Tags

   Hexahistidine

   Expression

   Bacterial

   Mutation

   Cys321->Ala321; Cys367->Ala367

   Available Since

   Aug. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLEX307_PDHA2S291D

   Plasmid

   #115193

   Purpose

   Lentiviral transduction and expression of PDHA2S291D into any mammalian cell

   Depositor

   Mohan Babu

   Article

   Jessulat et al Nucleic Acids Res. 2021 Jul 9. pii: 6318

   Insert

   PDHA2S291D (PDHA2 Human)
   

   Use

   Lentiviral

   Mutation

   p.S291D

   Promoter

   EF1alpha

   Available Since

   July 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLEX307_PDHA2S293D

   Plasmid

   #115194

   Purpose

   Lentiviral transduction and expression of PDHA2S293D into any mammalian cell

   Depositor

   Mohan Babu

   Article

   Jessulat et al Nucleic Acids Res. 2021 Jul 9. pii: 6318

   Insert

   PDHA2S293D (PDHA2 Human)
   

   Use

   Lentiviral

   Mutation

   p.S293D

   Promoter

   EF1alpha

   Available Since

   July 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLEX307_PDHA2S291D/S293D

   Plasmid

   #115195

   Purpose

   Lentiviral transduction and expression of PDHA2S291D/S293D into any mammalian cell

   Depositor

   Mohan Babu

   Article

   Jessulat et al Nucleic Acids Res. 2021 Jul 9. pii: 6318

   Insert

   PDHA2S291D/S293D (PDHA2 Human)
   

   Use

   Lentiviral

   Mutation

   p.S291D/S293D

   Promoter

   EF1alpha

   Available Since

   July 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. SYKA-c022

   Plasmid

   #175490

   Purpose

   Protein expression in bacterial cells. Tandem SH2 domains, M6-N269. Can be biotinylated.

   Depositor

   Opher Gileadi

   Article

   Bashore et al bioRxiv. 2023 Jul 29:2023.07.28.551026.

   Insert

   SYK (SYK Human)
   

   Tags

   AviTag and His6-TEV

   Expression

   Bacterial

   Available Since

   Jan. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCu-His6-Atg13[571–700] K683E, H685E, K686E-Ss(424)

    Plasmid

    #166855

    Purpose

    Expresses 6xHis tagged ATG13 [571–700] fragment mutated in K683E, H685E, K686E with 14 scramble amino acid sequence DIKLIDTVDLESCN under a Cu promoter in yeast

    Depositor

    Daniel Klionsky

    Article

    Gatica et al Autophagy. 2020 Jun;16(6):1007-1020. doi

    Insert

    Atg13[571–700] (ATG13 Budding Yeast)
    

    Tags

    6xHis

    Expression

    Yeast

    Mutation

    K683E, H685E, K686E

    Promoter

    pCu

    Available Since

    April 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCu-His6-Atg13[571–700] F641E, I645E-Ss(424)

    Plasmid

    #166854

    Purpose

    Expresses 6xHis tagged ATG13 [571–700] fragment mutated in F641E, I645E with 14 scramble amino acid sequence DIKLIDTVDLESCN under a Cu promoter in yeast

    Depositor

    Daniel Klionsky

    Article

    Gatica et al Autophagy. 2020 Jun;16(6):1007-1020. doi

    Insert

    Atg13[571–700] (ATG13 Budding Yeast)
    

    Tags

    6xHis

    Expression

    Yeast

    Mutation

    F641E, I645E

    Promoter

    pCu

    Available Since

    April 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCu-His6-Atg13[571–700] F641G, I645G-Ss(424)

    Plasmid

    #166853

    Purpose

    Expresses 6xHis tagged ATG13 [571–700] fragment mutated in F641G, I645G with 14 scramble amino acid sequence DIKLIDTVDLESCN under a Cu promoter in yeast

    Depositor

    Daniel Klionsky

    Article

    Gatica et al Autophagy. 2020 Jun;16(6):1007-1020. doi

    Insert

    Atg13[571–700] (ATG13 Budding Yeast)
    

    Tags

    6xHis

    Expression

    Yeast

    Mutation

    F641G, I645G

    Promoter

    pCu

    Available Since

    April 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. CPH3400 (YCp LEU2 Rpb1 A1529_G1534delInsLEVLFQGP)

    Plasmid

    #91806

    Purpose

    Yeast expression of S. cerevisiae Rpb1 with an internal PreScission protease site

    Depositor

    Chris Hill

    Article

    Sdano et al Elife. 2017 Aug 16;6. pii: 28723. doi: 1

    Insert

    RPO21 (RPO21 Budding Yeast)
    

    Expression

    Yeast

    Mutation

    Residues A1529-G1534 replaced with a PreScission …

    Promoter

    Endogenous Rpb1 promoter

    Available Since

    March 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. CPH3477 (YCp LEU2 Rpb1 P1455_E1456InsLEVLFQGP)

    Plasmid

    #91807

    Purpose

    Yeast expression of S. cerevisiae Rpb1 with an internal PreScission protease site

    Depositor

    Chris Hill

    Article

    Sdano et al Elife. 2017 Aug 16;6. pii: 28723. doi: 1

    Insert

    RPO21 (RPO21 Budding Yeast)
    

    Expression

    Yeast

    Mutation

    PreScission Protease site (LEVLFQGP) inserted bet…

    Promoter

    Endogenous Rpb1 promoter

    Available Since

    March 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. CPH3506 (pET151_Spt6 1247-1451 K1355A,K1435A)

    Plasmid

    #91818

    Purpose

    Bacterial expression of residues 1247-1451 from S. cerevisiae Spt6 with K1435A and K1355A mutations

    Depositor

    Chris Hill

    Article

    Sdano et al Elife. 2017 Aug 16;6. pii: 28723. doi: 1

    Insert

    SPT6 (SPT6 Budding Yeast)
    

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    changed lysine 1355 and lysine 1435 to alanines; …

    Promoter

    T7

    Available Since

    March 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. mWasabi-ADAP1

    Plasmid

    #163906

    Purpose

    Full length mouse ADAP1 with N terminal fluorescent tag for purification from insect cells

    Depositor

    Martin Loose

    Article

    Duellberg et al Proc Natl Acad Sci U S A. 2021 Jan 5;118

    Insert

    ADAP1 (Adap1 Mouse)
    

    Tags

    mWasabi fluorescent tag

    Expression

    Insect

    Promoter

    polyhedrin

    Available Since

    March 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. FUS_LC_charge_RtoK

    Plasmid

    #139127

    Purpose

    express FUS LC with hnRNPA2-like charge patterning where all R are changed to K

    Depositor

    Nicolas Fawzi

    Article

    Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

    Insert

    FUS (FUS Human)
    

    Expression

    Bacterial

    Mutation

    Add K and D to FUS LC so it has the same charge p…

    Available Since

    Dec. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pPICZ-His6-K2P4.1B-mCherry-StreptagII

    Plasmid

    #158744

    Purpose

    Expresses K2P4.1 isoform B with TEV protease cleavable N- and C-terminal affinity tags.

    Depositor

    Arthur Laganowsky

    Article

    Schrecke et al Nat Chem Biol. 2021 Jan;17(1):89-95. doi

    Insert

    K2P4.1-B (KCNK4 Human)
    

    Tags

    His6 and mCherry with Streptag II

    Expression

    Yeast

    Mutation

    Residues 1-290 and N-linked glycosylation sites r…

    Promoter

    AOX1

    Available Since

    Sept. 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLPC-Flag-SOCS1-Y80D

    Plasmid

    #129516

    Purpose

    Retroviral vector for the expression of human SOCS1 with a Y80D mutation and a FLAG tag in N-terminal

    Depositor

    Gerardo Ferbeyre

    Article

    Saint-Germain et al Cancer Res. 2019 Jul 1;79(13):3306-3319.

    Insert

    SOCS1 (SOCS1 Human)
    

    Use

    Retroviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Changed Tyrosine 80 to Aspartic acid

    Promoter

    CMV

    Available Since

    Sept. 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLPC-Flag-SOCS1-Y80F

    Plasmid

    #129517

    Purpose

    Retroviral vector for the expression of human SOCS1 with a Y80F mutation and a FLAG tag in N-terminal

    Depositor

    Gerardo Ferbeyre

    Article

    Saint-Germain et al Cancer Res. 2019 Jul 1;79(13):3306-3319.

    Insert

    SOCS1 (SOCS1 Human)
    

    Use

    Retroviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Changed Tyrosine 80 to Phenylalanine

    Promoter

    cmv

    Available Since

    Sept. 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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