We narrowed to 2,598 results for: ubiquitin

1. His-Precission-NEDD4L_C942S_pCDNA3.1(+)

   Plasmid

   #187729

   Purpose

   Express the catalytically inactive NEDD4L FL in mammalian cells

   Depositor

   Sandra Gabelli

   Article

   Wright et al J Biol Chem. 2024 Mar;300(3):105715. doi

   Insert

   NEDD4L FL with C874 mutated to a serine (NEDD4L Human)
   

   Use

   Tags

   His-HRV 3C site

   Expression

   Mammalian

   Mutation

   Cys874 mutated to a serine

   Promoter

   Available since

   Oct. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEGFPc3 K-RAS 4B

   Plasmid

   #236452

   Purpose

   transient overexpression of K-RAS 4B in mammalian cells

   Depositor

   Michele Pagano

   Article

   Kuchay et al Nat Struct Mol Biol. 2019 Jul;26(7):628-

   Insert

   K-RAS 4B (KRAS Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 21, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. TRIPZ-bioGEF-UBwt-PURO

   Plasmid

   #208045

   Purpose

   Enables inducible expression of low-affinity Avitag-Ubwt; to perform bioE3; selection with puromycin

   Depositor

   Rosa Barrio, James Sutherland

   Article

   Barroso-Gomila et al Nat Commun. 2023 Nov 23;14(1):7656. doi:

   Insert

   Ub (UBC Human)
   

   Use

   Lentiviral

   Tags

   Low-affinity Avitag

   Expression

   Mutation

   Promoter

   tetO/UBC

   Available since

   Nov. 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. Lenti-EFS-BirAopt-GSQ-RBXN-P2A-BLAST

   Plasmid

   #208048

   Purpose

   Enables constitutive expression of BirAopt alone; RBXN represents a EcoR1-BsiW1-Xba1-Not1 polylinker; to perform bioE3; selection with blasticidin

   Depositor

   Rosa Barrio, James Sutherland

   Article

   Barroso-Gomila et al Nat Commun. 2023 Nov 23;14(1):7656. doi:

   Insert

   BirAopt
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   BirAopt is human-codon-optimized BirA (PMID 15707…

   Promoter

   EFS

   Available since

   Nov. 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. His-MBP human SPOP (aa 28-337)

   Plasmid

   #52294

   Purpose

   bacterial expression of human SPOP (aa 28-337) fused to His-MBP

   Depositor

   Brenda Schulman

   Article

   Zhuang et al Mol Cell. 2009 Oct 9;36(1):39-50. doi: 1

   Insert

   SPOP (SPOP Human)
   

   Use

   Tags

   His-MBP and TEV

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   April 11, 2014

   Availability

   Academic Institutions and Nonprofits only

6. pcDNA3-Flag-RNF168

   Plasmid

   #133976

   Purpose

   mammalian expression vector of Flag tagged wild type RNF168

   Depositor

   Daniel Durocher

   Article

   Stewart et al Cell. 2009 Feb 6;136(3):420-34. doi: 10.

   Insert

   RNF168 (RNF168 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 4, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. Lenti-EFS-BirAopt-GSQ-RNF4wt-P2A-BLAST

   Plasmid

   #208046

   Purpose

   Enables constitutive expression of N-terminal BirAopt-fused RNF4wt; to perform bioE3; selection with blasticidin

   Depositor

   Rosa Barrio, James Sutherland

   Article

   Barroso-Gomila et al Nat Commun. 2023 Nov 23;14(1):7656. doi:

   Insert

   RNF4 (RNF4 Human)
   

   Use

   Lentiviral

   Tags

   BirAopt

   Expression

   Mutation

   Promoter

   EFS

   Available since

   Nov. 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3.1-N-Myc_535-1102 USP7

   Plasmid

   #131246

   Purpose

   Mammalian expression of the five USP7 UBL domains with an N-terminal Myc tag.

   Depositor

   Roger Woodgate

   Article

   Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d

   Insert

   Ubiquitin carboxyl-terminal hydrolase 7 (USP7 Human)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Expresses USP7 amino acids 535-1102. This truncat…

   Promoter

   Available since

   Dec. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. His Puc (aa 1-393)

   Plasmid

   #52295

   Purpose

   Bacterial expression of His tagged Drosophila Puc fragment

   Depositor

   Brenda Schulman

   Article

   Zhuang et al Mol Cell. 2009 Oct 9;36(1):39-50. doi: 1

   Insert

   Puc (puc Fly)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 24, 2018

   Availability

   Academic Institutions and Nonprofits only

10. pDONR-53BP1 1-1972 WT

    Plasmid

    #53446

    Purpose

    Recombinational donor/master vector

    Depositor

    Daniel Durocher

    Article

    Fradet-Turcotte et al Nature. 2013 Jul 4;499(7456):50-4. doi:

    Insert

    53BP1 (TP53BP1 Human)
    

    Use

    Gateway

    Tags

    Expression

    Mutation

    siRNA resistant and mutations A128V, T720A, A1347…

    Promoter

    Available since

    April 10, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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