We narrowed to 7,221 results for: ALP

1. pMA3379

   Plasmid

   #46874

   Purpose

   A lentiviral vector for the doxycycline-inducible expression of soluble guanylate cyclase1 alpha3

   Depositor

   Mikhail Alexeyev, Troy Stevens

   Article

   Ochoa et al J Biol Chem. 2012 Jul 20;287(30):25407-1

   Insert

   soluble guanylate cyclase 1alpha3 (Gucy1a3 Rat)
   

   Use

   Lentiviral

   Tags

   Myc

   Expression

   Mutation

   Promoter

   TRE-Tight

   Available since

   Dec. 11, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. PLK3 335-646-pEGFP

   Plasmid

   #23267

   Depositor

   Wei Dai

   Article

   Yang et al Cancer Res. 2008 Jun 1. 68(11):4077-85.

   Insert

   PLK3 (PLK3 Human)
   

   Use

   Tags

   pEGFP

   Expression

   Mammalian

   Mutation

   aa 335-646

   Promoter

   Available since

   Dec. 20, 2012

   Availability

   Academic Institutions and Nonprofits only

3. FUW-M2-PAK2 S20A

   Plasmid

   #31664

   Depositor

   Anne Brunet

   Article

   Banko et al Mol Cell. 2011 Dec 23;44(6):878-92. Epub

   Insert

   p21-activated protein kinase 2 S20A (PAK2 Human)
   

   Use

   Lentiviral

   Tags

   M2

   Expression

   Mammalian

   Mutation

   Changed Serine 20 to Alanine

   Promoter

   Available since

   April 12, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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4. C25-pLAC

   Plasmid

   #45283

   Purpose

   lacritin-intein fusion plasmid coding for truncated lacritin without signal peptide

   Depositor

   Gordon Laurie

   Article

   Wang et al J Cell Biol. 2006 Aug 28;174(5):689-700.

   Insert

   pLAC-C25 (LACRT Human)
   

   Use

   Protein expression vector

   Tags

   Intein

   Expression

   Bacterial

   Mutation

   contains aa20-113 of lacritin

   Promoter

   T7

   Available since

   Sept. 25, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. SEMA4-bio-His

   Plasmid

   #51827

   Purpose

   Expresses full-length Semaphorin-4D precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

   Depositor

   Gavin Wright

   Article

   Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

   Insert

   SEMA4 (SEMA4D Human)
   

   Use

   Tags

   His tag, enzymatic biotinylation sequence, and ra…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. CFP-PBD(H83,86L)

   Plasmid

   #11402

   Depositor

   Joel Swanson

   Article

   Hoppe et al Mol Biol Cell 2004 Aug;15(8):3509-19. Ep

   Insert

   p21-activated kinase 1 (PAK1 Human)
   

   Use

   Tags

   CFP

   Expression

   Mammalian

   Mutation

   Obtained with Histidine residues 83 and 86 change…

   Promoter

   Available since

   Feb. 21, 2007

   Availability

   Academic Institutions and Nonprofits only

7. pBS/U6/RNAi SadBs shRNA mouse

   Plasmid

   #67152

   Purpose

   pBS/U6 containing shRNA to mouse SADBs

   Depositor

   Maria Alvarado-Kristensson, Ana Carrera

   Article

   Alvarado-Kristensson et al Nat Cell Biol. 2009 Sep;11(9):1081-92. d

   Insert

   BRSK1 (Brsk1 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Nov. 16, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pFBD-Akt1(T308A/S473A)

   Plasmid

   #86578

   Purpose

   Expresses human full-length Akt1(T308A/S473A) phosphomutant

   Depositor

   Thomas Leonard, Ivan Yudushkin

   Article

   Ebner et al Mol Cell. 2017 Feb 2;65(3):416-431.e6. d

   Insert

   Akt1 (AKT1 Human)
   

   Use

   Tags

   10xHis

   Expression

   Insect

   Mutation

   T308A/S473A phosphomutant

   Promoter

   Available since

   April 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. p3A9cbetaTCR.20beta

   Plasmid

   #69592

   Purpose

   TCR beta chain genomic transgenic construct for creating OT-II mice

   Depositor

   Francis R. Carbone

   Article

   Barnden et al Immunol Cell Biol. 1998 Feb;76(1):34-40.

   Insert

   TCR beta VJ region from clone 1.1 (Tcrb Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

10. CD58-bio-His

    Plasmid

    #53153

    Purpose

    Expresses full-length CD58 ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

    Depositor

    Gavin Wright

    Article

    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

    Insert

    CD58 (CD58 Human)
    

    Use

    Tags

    His tag, enzymatic biotinylation sequence, and ra…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSL0721 (pDonor_L1-105)

    Plasmid

    #130645

    Purpose

    Encodes a mini-transposon derived from V. cholerae Tn6677 CAST, with CmR cargo gene and shortened left end. Total transposon size = 933 bp.

    Depositor

    Samuel H. Sternberg

    Article

    Klompe et al Nature. 2019 Jul;571(7764):219-225. doi:

    Insert

    VchCAST donor DNA (short L)
    

    Use

    CRISPR; Transposon

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSL0376 (His-MBP-StrepII-TniQ)

    Plasmid

    #130641

    Purpose

    Expresses V. cholerae CAST TniQ from a T7 promoter, with N-terminal His10-MBP-TEVsite-StrepII for purification.

    Depositor

    Samuel H. Sternberg

    Article

    Klompe et al Nature. 2019 Jul;571(7764):219-225. doi:

    Insert

    VchTniQ
    

    Use

    CRISPR; Transposon

    Tags

    His10-MBP-TEVsite-StrepII on TniQ

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pVENUS-N1-FNTA

    Plasmid

    #86859

    Purpose

    To express FNTA with a Venus tag at C terminus.

    Depositor

    Yihong Ye

    Article

    Xu et al Cell Discov. 2016 Nov 8;2:16040. eCollec

    Insert

    FNTA (FNTA Human)
    

    Use

    Tags

    Venus

    Expression

    Mammalian

    Mutation

    The sequence of the insert is isoform 2, which di…

    Promoter

    CMV

    Available since

    April 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pHR-FRB:Akt1(D274A)

    Plasmid

    #86634

    Purpose

    Expresses FRB-tagged human Akt1(D274A) kinase-inactive mutant

    Depositor

    Thomas Leonard, Ivan Yudushkin

    Article

    Ebner et al Mol Cell. 2017 Feb 2;65(3):416-431.e6. d

    Insert

    FRB-Akt1(D274A) (AKT1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSL0715 (pDonor_R1-57)

    Plasmid

    #130644

    Purpose

    Encodes a mini-transposon derived from V. cholerae Tn6677 CAST, with CmR cargo gene and shortened right end. Total transposon size = 910 bp.

    Depositor

    Samuel H. Sternberg

    Article

    Klompe et al Nature. 2019 Jul;571(7764):219-225. doi:

    Insert

    VchCAST donor DNA (short R)
    

    Use

    CRISPR; Transposon

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. MBC2-PuroIII

    Plasmid

    #153420

    Purpose

    MSCV backbone with murine TCR-alpha signal peptide and murine TCR-beta2 constant region (TRBC2) for expression of TCR genes

    Depositor

    Maki Nakayama

    Article

    Anderson et al Proc Natl Acad Sci U S A. 2021 Oct 12;11

    Insert

    murine TCR-alpha signal peptide and murine TCR-beta2 constant region (TRBC2)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pFlg167

    Plasmid

    #101019

    Purpose

    fluorescent, functional, glutamate receptor subunit

    Depositor

    Thomas Hughes

    Article

    Sheridan et al Neuroscience. 2006 Aug 25;141(2):837-49.

    Insert

    GluR 1 (Gria1 Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    fluorescent protein inserted into subunit

    Promoter

    cmv

    Available since

    Sept. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGL3-CMV-Cdc42 3'UTR

    Plasmid

    #51389

    Depositor

    Narry Kim

    Article

    Park et al Nat Struct Mol Biol. 2009 Jan;16(1):23-9

    Insert

    Cdc42 3'UTR (CDC42 Human)
    

    Use

    Luciferase

    Tags

    Luciferase

    Expression

    Mutation

    Promoter

    CMV

    Available since

    March 31, 2014

    Availability

    Academic Institutions and Nonprofits only

19. pmCherry-PH1(K14A)

    Plasmid

    #86600

    Purpose

    Expresses mCherry-tagged PH(K14A) domain of human Akt1

    Depositor

    Thomas Leonard, Ivan Yudushkin

    Article

    Ebner et al Mol Cell. 2017 Feb 2;65(3):416-431.e6. d

    Insert

    K14A mutant of Akt1 PH domain (AKT1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA-Flag-Trib1

    Plasmid

    #131156

    Purpose

    Mammalian expression of flag-tagged mouse TRIB1

    Depositor

    Karen Keeshan, Warren Pear

    Article

    Dedhia et al Blood. 2010 Aug 26;116(8):1321-8. doi: 1

    Insert

    Trib1 (Trib1 Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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